Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 285,882 T→G 5.0% I163S (ATC→AGC)  yagG → CP4‑6 prophage; putative sugar transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913285,8820TG 5.0% 645.0 / 22.3 186I163S (ATC→AGC) yagGCP4‑6 prophage; putative sugar transporter
Reads supporting (aligned to +/- strand):  ref base T (85/91);  new base G (5/5);  total (90/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01

Only 100 of 191 total aligned reads displayed.

TACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCG  >  NC_000913/285734‑286030
                                                                                                                                                    |                                                                                                                                                    
tACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCAt                                                                                                                                                      >  1:247457/1‑149 (MQ=255)
       tcCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTg                                                                                                                                               >  2:1541966/1‑149 (MQ=255)
       tcCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCATCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCACTCGCTATCAGCGGCATCGCGCTg                                                                                                                                               >  1:828694/1‑149 (MQ=255)
              ccTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTgg                                                                                                                                        >  3:1278786/1‑149 (MQ=255)
              ccTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTgg                                                                                                                                        >  1:482055/1‑149 (MQ=255)
              ccTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCATGCACTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTgg                                                                                                                                        >  3:1626045/1‑149 (MQ=255)
                tGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTg                                                                                                                                      >  3:688402/1‑149 (MQ=255)
                tGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTg                                                                                                                                      <  4:1671028/149‑1 (MQ=255)
                gaagaagcagacgtagagcagcaccacgccgctcagccccagcacgcacatggcgccgaagtagcccacctgctcgtcccctttgccgatgatgctcaccaGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTg                                                                                                                                      >  4:1322160‑M1/102‑149 (MQ=255)
                 ggTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGa                                                                                                                                     <  3:176638/149‑1 (MQ=255)
                  gTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAg                                                                                                                                    >  4:1259221/1‑149 (MQ=255)
                   tCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCAt                                                                                                                                                      >  2:1733983/1‑130 (MQ=255)
                   tCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCAt                                                                                                                                                      <  1:1733983/130‑1 (MQ=255)
                     tACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCGGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGcat                                                                                                                                 >  3:1239864/1‑149 (MQ=255)
                      gcagacgtagagcagcaccacgccgctcagccccagcacgcacatggcgccgaagtagcccacctgctcgtcccctttgccgatgatgctcaccaGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTg                                                                                                                                         <  2:93022‑M1/45‑1 (MQ=255)
                      gcagacgtagagcagcaccacgccgctcagccccagcacgcacatggcgccgaagtagcccacctgctcgtcccctttgccgatgatgctcaccaGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTg                                                                                                                                         >  1:93022‑M1/96‑140 (MQ=255)
                      acacCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGcatc                                                                                                                                >  3:945428/1‑149 (MQ=255)
                          cTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGa                                                                                                                                     >  3:1116390/1‑140 (MQ=255)
                          cTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCg                                                                                                                            <  4:1228857/149‑1 (MQ=255)
                           ttCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCgg                                                                                                                           >  3:1565806/1‑149 (MQ=255)
                              gTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCaa                                                                                                                        >  3:1387269/1‑149 (MQ=255)
                               ttAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCaaa                                                                                                                       >  2:1646411/1‑149 (MQ=255)
                               ttAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCaaa                                                                                                                       >  4:436555/1‑149 (MQ=255)
                               ttAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCAGCGCGATGCCGCt                                                                                                                                          >  3:216907/1‑130 (MQ=255)
                               ttAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCAGCGCGATGCCGCt                                                                                                                                          <  4:216907/130‑1 (MQ=255)
                                tAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAg                                                                                                                      <  3:817606/149‑1 (MQ=255)
                                tAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAg                                                                                                                      <  1:110099/149‑1 (MQ=255)
                                 aaCGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAgg                                                                                                                     >  1:1345778/1‑149 (MQ=255)
                                  aCGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGACTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAggg                                                                                                                    <  3:429864/149‑1 (MQ=255)
                                   cGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAgggg                                                                                                                   <  1:1183960/149‑1 (MQ=255)
                                    gTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGa                                                                                                                  <  4:106763/149‑1 (MQ=255)
                                    gTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGa                                                                                                                  >  2:224062/1‑149 (MQ=255)
                                     tGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCGCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGTAAAGGGGAc                                                                                                                 <  2:807010/149‑1 (MQ=255)
                                       ccGTACTGCGCCATGCCGGGCGTCATCCCCGCCGACCCGAAAGAGCATCACACCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGa                                                                                                               <  2:658204/149‑1 (MQ=255)
                                       ccGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGa                                                                                                               <  3:898068/149‑1 (MQ=255)
                                          tACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCa                                                                                                            >  3:1395936/1‑149 (MQ=255)
                                              gcgcCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTg                                                                                                        <  3:1158777/149‑1 (MQ=255)
                                              gcgcCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTg                                                                                                        <  2:1345778/149‑1 (MQ=255)
                                              gcgcCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTg                                                                                                        <  3:26559/149‑1 (MQ=255)
                                               cgcCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTgg                                                                                                       <  4:1714853/149‑1 (MQ=255)
                                                 ccATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGc                                                                                                     <  4:1367349/149‑1 (MQ=255)
                                                  cATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCgg                                                                                                                           <  2:1225199/126‑1 (MQ=255)
                                                            gTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGa                                                                                                                  <  2:1716525/125‑1 (MQ=255)
                                                            gTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGa                                                                                                                  >  1:1716525/1‑125 (MQ=255)
                                                             tcatcaCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGcc                                                                                         >  4:1285000/1‑149 (MQ=255)
                                                                          cccGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTAc                                                                                                  >  2:1699200/1‑127 (MQ=255)
                                                                          cccGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTAc                                                                                                  <  1:1699200/127‑1 (MQ=255)
                                                                          cccGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTgg                                                                            <  4:591114/149‑1 (MQ=255)
                                                                            cGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTgggg                                                                          <  1:671214/149‑1 (MQ=255)
                                                                            cGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTgggg                                                                          >  1:1752811/1‑149 (MQ=255)
                                                                             gAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGc                                                                         >  2:837259/1‑149 (MQ=255)
                                                                                       cACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCgt                                                               <  1:300641/149‑1 (MQ=255)
                                                                                        aCGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGTGTGCTGGGGCTGAGCGGCgtg                                                              <  4:360203/149‑1 (MQ=255)
                                                                                          gCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCgtggt                                                            >  1:591231/1‑149 (MQ=255)
                                                                                           cccTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCgtggtg                                                           >  4:1534577/1‑149 (MQ=255)
                                                                                              tGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATcgc                                                                                                                                                   >  2:1493297/1‑58 (MQ=255)
                                                                                              tGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATcgc                                                                                                                                                   <  1:1493297/58‑1 (MQ=255)
                                                                                              tGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGtgctg                                                        <  4:705397/149‑1 (MQ=255)
                                                                                               gCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGtgctgc                                                       >  3:1310148/1‑149 (MQ=255)
                                                                                                cAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTgctgct                                                      <  2:263174/149‑1 (MQ=255)
                                                                                                 aGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGctc                                                     <  1:1234855/149‑1 (MQ=255)
                                                                                                   tCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTa                                                   <  3:375660/149‑1 (MQ=255)
                                                                                                   tCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTa                                                   >  3:512557/1‑149 (MQ=255)
                                                                                                     cTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACg                                                 >  3:135664/1‑149 (MQ=255)
                                                                                                     cTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACg                                                 >  2:434950/1‑149 (MQ=255)
                                                                                                     cTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACg                                                 >  4:327271/1‑149 (MQ=255)
                                                                                                      tGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGt                                                >  4:1650600/1‑149 (MQ=255)
                                                                                                      tGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGt                                                >  2:1136722/1‑149 (MQ=255)
                                                                                                           cttcttCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGct                                           >  2:406724/1‑149 (MQ=255)
                                                                                                            ttcttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGctt                                          <  2:1163446/149‑1 (MQ=255)
                                                                                                              cttcCTGGCGGCGGAGGGCTGGCTCGGGAGCAGCGGCATCGCGCTGCCGCTGGTGCGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGGTGAGCGGCGTGGTGCTGCTCTACGACTGcttct                                        <  2:76659/149‑1 (MQ=255)
                                                                                                               ttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGcttctt                                       <  1:434614/149‑1 (MQ=255)
                                                                                                                tcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGa                                                                      <  1:1513779/117‑1 (MQ=255)
                                                                                                                 ccTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCa                                     <  1:1478691/149‑1 (MQ=255)
                                                                                                                  cTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCAc                                    <  4:524519/149‑1 (MQ=255)
                                                                                                                  cTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCAc                                    >  4:1348742/1‑149 (MQ=255)
                                                                                                                    ggcggcggcGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGa                                  <  4:1371450/149‑1 (MQ=255)
                                                                                                                    ggcggcggcGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGa                                  <  4:682455/149‑1 (MQ=255)
                                                                                                                         CGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCaaa                             >  4:502334‑M1/1‑149 (MQ=255)
                                                                                                                             gggCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTAc                                                                                                  >  3:640608/1‑76 (MQ=255)
                                                                                                                             gggCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTAc                                                                                                  <  4:640608/76‑1 (MQ=255)
                                                                                                                               gctcgctcGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCaaa                             >  4:1145493/1‑143 (MQ=255)
                                                                                                                               gctcgctcGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAgcgc                       <  1:1040591/149‑1 (MQ=255)
                                                                                                                               gctcgctcGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAgcgc                       <  3:1419711/149‑1 (MQ=255)
                                                                                                                                  cgctcgctATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTac                    <  2:482055/149‑1 (MQ=255)
                                                                                                                                    ctcgctATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTTCTGCTCTACGTCTGCTTCTTCACGACCAAAGAgcgc                       <  3:531961/144‑1 (MQ=255)
                                                                                                                                     tcgctATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACAcc                 >  1:1040858/1‑149 (MQ=255)
                                                                                                                                        tcaccAGCGGCAGCGCGATGCCGCTGATGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCttt              >  1:277145/5‑149 (MQ=255)
                                                                                                                                        tcaccAGCGGCAGCGCGATGCCGCTGATGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCttt              >  1:717544/5‑149 (MQ=255)
                                                                                                                                        ctATCAGCGGCATCGCGCTGCCGCTCCTGCGCATCACCGGCAAAGGGGACGAGCAGGTGCGCTGCTTCGTCGCCATGTGCATGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCCTCTTCACGACCAAAGAGCGCTACACCttt              <  4:1445187/149‑1 (MQ=255)
                                                                                                                                          aTCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGa            <  4:399830/149‑1 (MQ=255)
                                                                                                                                           ccAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGc                                                                         >  1:364709/2‑87 (MQ=39)
                                                                                                                                           ccAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGc                                                                         <  2:364709/86‑1 (MQ=255)
                                                                                                                                             aGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGt         >  2:1720147/1‑149 (MQ=255)
                                                                                                                                                 gCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAg     >  3:472973/1‑149 (MQ=255)
                                                                                                                                                   aTCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGcc   >  4:1312870/1‑149 (MQ=255)
                                                                                                                                                   aTCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGcc   >  2:806218/1‑149 (MQ=255)
                                                                                                                                                   aTCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGcc   >  4:1618975/1‑149 (MQ=255)
                                                                                                                                                    tCGCGCTGCCTCTGGTGAGCATCATCGGCAAAGGGGACAGGCAGGTGGGCTACTTCGGCGGCATGTGCTTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTGCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCg  >  4:203354/1‑149 (MQ=255)
                                                                                                                                                    tCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCg  <  2:1470747/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCG  >  NC_000913/285734‑286030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: