Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,982 G→C 6.2% intergenic (+28/+32) yhcG → / ← yhcH DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily/DUF386 family protein, cupin superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,9820GC 6.2% 625.1 / 24.7 177intergenic (+28/+32)yhcG/yhcHDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily/DUF386 family protein, cupin superfamily
Reads supporting (aligned to +/- strand):  ref base G (97/69);  new base C (6/5);  total (103/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

Only 100 of 183 total aligned reads displayed.

GTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGG  >  NC_000913/3368836‑3369130
                                                                                                                                                  |                                                                                                                                                    
gTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAgtg                                                                                                                                                    >  3:1284419/1‑149 (MQ=255)
gTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAgtg                                                                                                                                                    >  1:541309/1‑149 (MQ=255)
 tttGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAgtgt                                                                                                                                                   <  4:452328/149‑1 (MQ=255)
      cTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCa                                                                                                                                              <  1:599958/149‑1 (MQ=255)
      cTAACACCGGTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCa                                                                                                                                              >  4:527376/1‑149 (MQ=255)
       tAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAg                                                                                                                                             >  2:116127/1‑149 (MQ=255)
         acacCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGaa                                                                                                                                           >  3:1579720/1‑149 (MQ=255)
           acCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATc                                                                                                                                         <  1:902019/149‑1 (MQ=255)
           acCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATc                                                                                                                                         <  3:1614761/149‑1 (MQ=255)
           acCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATc                                                                                                                                         >  3:578037/1‑149 (MQ=255)
           acCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATc                                                                                                                                         <  3:987502/149‑1 (MQ=255)
                  tGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTaaaa                                                                                                                                  <  2:138534/149‑1 (MQ=255)
                  tGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTaaaa                                                                                                                                  <  4:1310965/149‑1 (MQ=255)
                  tGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTaaaa                                                                                                                                  <  2:1661604/149‑1 (MQ=255)
                   ggCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAAc                                                                                                                                 >  2:1184038/1‑149 (MQ=255)
                      aaGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGaa                                                                                                                              >  3:703006/1‑149 (MQ=255)
                      aaGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGaa                                                                                                                              <  3:17138/149‑1 (MQ=255)
                            atatAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGt                                                                                                                        >  1:1224808/1‑149 (MQ=255)
                             tataAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACCGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGtt                                                                                                                       >  1:182741/1‑149 (MQ=255)
                               taAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGtttt                                                                                                                     >  3:421003/1‑149 (MQ=255)
                                aaGATGCAACTACCTGATGAGAAGCGGCTCGCAGATGTACTCGGGCGAAAACAGGCGGTGGTAGAGGAAGCCTATAGACGCCGGTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTGTGCAGAATCTTTAAAACGAACTAAGTTTTa                                                                                                                    >  4:259416/1‑149 (MQ=255)
                                aaGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTTCAGAATCTTTAAAACGAACTAAGTTTTa                                                                                                                    >  2:270324/1‑149 (MQ=255)
                                aaGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTa                                                                                                                    >  1:1007667/1‑149 (MQ=255)
                                 aGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTaa                                                                                                                   <  4:1563915/149‑1 (MQ=255)
                                 aGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTaa                                                                                                                   <  3:1080509/149‑1 (MQ=255)
                                  gATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAg                                                                                                                  >  4:980280/1‑149 (MQ=255)
                                   aTGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCttt                                                                                                                                      <  1:932286/128‑1 (MQ=255)
                                   aTGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCttt                                                                                                                                      >  2:932286/1‑128 (MQ=255)
                                   aTGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGc                                                                                                                 <  1:1134360/149‑1 (MQ=255)
                                     gCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGATCTAAGTTTTAAGCCa                                                                                                               >  1:289617/1‑149 (MQ=255)
                                        aCTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTa                                                                                                            >  3:1226834/1‑149 (MQ=255)
                                              tgatgaGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAg                                                                                                      >  1:1291569/1‑149 (MQ=255)
                                               gatgaGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGaa                                                                                                                              >  1:1577854/1‑124 (MQ=255)
                                               gatgaGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGaa                                                                                                                              <  2:1577854/124‑1 (MQ=255)
                                                  gagaAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCCTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCtt                                                                                                  >  1:1722311/1‑149 (MQ=255)
                                                   agaAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTa                                                                                                 >  2:879647/1‑149 (MQ=255)
                                                    gaAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTaa                                                                                                >  3:497723/1‑149 (MQ=255)
                                                     acaCGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAAc                                                                                               <  4:1362820/147‑1 (MQ=255)
                                                     aaaCGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAAc                                                                                               <  4:20440/149‑1 (MQ=255)
                                                     aaaCGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAAc                                                                                               <  2:1149597/149‑1 (MQ=255)
                                                      aaCGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATTAGCCTTAAcc                                                                                              >  3:526448/1‑149 (MQ=255)
                                                       aCGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCt                                                                                             >  2:202040/1‑149 (MQ=255)
                                                          aCTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTca                                                                                          <  1:193386/149‑1 (MQ=255)
                                                            tCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcacc                                                                                        >  4:145801/1‑149 (MQ=255)
                                                            tCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcacc                                                                                        >  1:439012/1‑149 (MQ=255)
                                                            tCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACACTGTCTGCAGAAtt                                                                                                                                         <  2:1696532/100‑2 (MQ=255)
                                                              gCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcaccac                                                                                      >  4:402500/1‑149 (MQ=255)
                                                                     aaCTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTaa                                                                                                                   <  2:1450285/113‑1 (MQ=255)
                                                                     aaCTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTaa                                                                                                                   >  1:1450285/1‑113 (MQ=255)
                                                                      aCTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCtttt                                                                              <  1:1258404/149‑1 (MQ=255)
                                                                       cTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCa                                                                                                       >  2:368672/1‑123 (MQ=255)
                                                                       cTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCttttt                                                                             >  3:751145/1‑149 (MQ=255)
                                                                       cTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCttttt                                                                             <  1:1052340/149‑1 (MQ=255)
                                                                        tCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTaa                                                                                                                   >  1:1479687/1‑110 (MQ=255)
                                                                        tCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTaa                                                                                                                   <  2:1479687/110‑1 (MQ=255)
                                                                        tCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcac                                                                                         >  3:1444129/1‑136 (MQ=255)
                                                                        tCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcac                                                                                         <  4:516870/136‑1 (MQ=255)
                                                                        tCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTa                                                                            >  4:742986/1‑149 (MQ=255)
                                                                         cGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTaa                                                                           <  1:981828/149‑1 (MQ=255)
                                                                          gTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAt                                                                          >  4:1163115/1‑149 (MQ=255)
                                                                           ttCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAtc                                                                         >  1:830948/1‑149 (MQ=255)
                                                                           ttCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAtc                                                                         >  4:626442/1‑149 (MQ=255)
                                                                                  acaGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAg                                                                  >  3:606343/1‑149 (MQ=255)
                                                                                         gTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGcc                                                           <  3:894264/149‑1 (MQ=255)
                                                                                           gCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGa                                                         >  4:268276/1‑149 (MQ=255)
                                                                                             tAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACa                                                       >  1:756501/1‑149 (MQ=255)
                                                                                              agagGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACaa                                                      <  3:1185690/149‑1 (MQ=255)
                                                                                              agagGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAAACAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACaa                                                      <  3:1563480/149‑1 (MQ=255)
                                                                                                agGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACg                                                    >  3:24332/1‑149 (MQ=255)
                                                                                                  gAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTc                                                                                           >  4:1424738/1‑108 (MQ=255)
                                                                                                  gAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCACTAAATCAGCCTTAACCTTc                                                                                           <  3:1424738/108‑1 (MQ=255)
                                                                                                  gAACGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAAACTTCACCACAACCTTTTTAATCTCTCCAGACTCGCCGACAACGCa                                                  >  1:124379/1‑149 (MQ=255)
                                                                                                     ggCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATcc                                               >  2:698807/1‑149 (MQ=255)
                                                                                                       cTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGATCTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTg                                             <  2:289617/149‑1 (MQ=255)
                                                                                                       cTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACACCCCTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTg                                             <  2:501718/149‑1 (MQ=255)
                                                                                                         ataGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGt                                           >  1:1656582/1‑149 (MQ=255)
                                                                                                          taGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAGCGCATCCTGGtt                                          <  2:1229960/149‑1 (MQ=255)
                                                                                                           aGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGttt                                         >  4:838268/1‑149 (MQ=255)
                                                                                                             aCGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTcc                                                                    >  4:435957/1‑120 (MQ=255)
                                                                                                             aCGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTAt                                       >  2:984670/1‑149 (MQ=255)
                                                                                                             aCGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTAt                                       >  2:1377409/1‑149 (MQ=255)
                                                                                                               gCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGc                                                                                                     <  2:774667/85‑1 (MQ=255)
                                                                                                                       tttCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCAcc                             >  2:275993/1‑149 (MQ=255)
                                                                                                                           aGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGc                         <  3:889328/149‑1 (MQ=255)
                                                                                                                                     aaTTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCtt                                                                                            >  3:1330996/1‑72 (MQ=255)
                                                                                                                                     aaTTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCtt                                                                                            <  4:1330996/72‑1 (MQ=255)
                                                                                                                                        tCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGa            <  2:1290905/149‑1 (MQ=255)
                                                                                                                                         cTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTcc                                                                    <  2:702141/92‑1 (MQ=255)
                                                                                                                                         cTGCAGACACTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAt                                                                          >  4:540163/1‑86 (MQ=255)
                                                                                                                                          tGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAAc          >  1:844129/1‑149 (MQ=255)
                                                                                                                                          tGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAAc          >  1:1635340/1‑149 (MQ=255)
                                                                                                                                          tGCAGACACTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAAc          >  3:76512/1‑149 (MQ=255)
                                                                                                                                           gCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcacca                                                                                       >  3:341511/1‑71 (MQ=255)
                                                                                                                                             aGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACAtt       >  3:682625/1‑149 (MQ=255)
                                                                                                                                              gACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTc      >  1:39047/1‑149 (MQ=255)
                                                                                                                                               aCAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTcc     >  3:1539687/1‑149 (MQ=255)
                                                                                                                                                cAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTccc    <  1:698807/149‑1 (MQ=255)
                                                                                                                                                 aGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGt                                           >  4:1199274/1‑109 (MQ=255)
                                                                                                                                                 aGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGc                                     >  1:1159230/1‑115 (MQ=255)
                                                                                                                                                  gtgtCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCgg  >  2:621473/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGG  >  NC_000913/3368836‑3369130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: