Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,886,757 T→C 5.0% intergenic (+35/‑71) yidC → / → mnmE membrane protein insertase/tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,886,7570TC 5.0% 656.9 / 14.7 199intergenic (+35/‑71)yidC/mnmEmembrane protein insertase/tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase
Reads supporting (aligned to +/- strand):  ref base T (102/87);  new base C (6/4);  total (108/91)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

Only 100 of 205 total aligned reads displayed.

GTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTCCGGC  >  NC_000913/3886610‑3886905
                                                                                                                                                   |                                                                                                                                                    
      tGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGAc                                                                                                                                                  <  1:1132768/146‑1 (MQ=255)
      tGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGAc                                                                                                                                                  >  2:1132768/1‑146 (MQ=255)
      tGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGc                                                                                                                                               >  4:1708470/1‑149 (MQ=255)
        cTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAACTGACCGccc                                                                                                                                             <  2:1171257/149‑2 (MQ=255)
         tGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtt                                                                                                                                            >  4:271454/1‑149 (MQ=255)
           tACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAACATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                          >  1:306926/1‑149 (MQ=255)
           tACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                          <  2:16148/149‑1 (MQ=255)
           tACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                          <  1:261997/149‑1 (MQ=255)
              tataTCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttc                                                                                                                                       >  2:135733/1‑149 (MQ=255)
               atatCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttct                                                                                                                                      <  2:306926/149‑1 (MQ=255)
                tatCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttctt                                                                                                                                     <  3:1551874/149‑1 (MQ=255)
                tatCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttctt                                                                                                                                     <  1:542795/149‑1 (MQ=255)
                   cGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttcttttc                                                                                                                                  <  1:518829/149‑1 (MQ=255)
                    gTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCg                                                                                                                                 >  3:300567/1‑149 (MQ=255)
                    gTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCg                                                                                                                                 >  3:1719241/1‑149 (MQ=255)
                      cAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTa                                                                                                                               <  2:1104526/149‑1 (MQ=255)
                         cAAACTGGTAGCCATTATTCTGCAGCAGTTGATTTACCGTGGTCTGCATAAACGTGGCCTGCATAGCCGCGAGAAGAAAAACGACTTATCCGGTGAGTTTCCGCTAAAATAAGGGCGGTGAGTTGACCGCCTTCTTTCTTTTCGTAgtc                                                                                                                            <  2:527299/149‑3 (MQ=255)
                          aaCCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGc                                                                                                                           >  4:476461/1‑149 (MQ=255)
                             cTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGa                                                                                                                        <  3:607992/149‑1 (MQ=255)
                             cTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGa                                                                                                                        <  3:578408/149‑1 (MQ=255)
                             cTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGa                                                                                                                        <  2:993046/149‑1 (MQ=255)
                             cTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGa                                                                                                                        <  3:1047540/149‑1 (MQ=255)
                             cTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGAATCGGTGAGCTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGa                                                                                                                        >  2:288740/1‑149 (MQ=255)
                              tGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGtt                                                                                                                                                     <  4:609730/119‑1 (MQ=255)
                              tGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGAt                                                                                                                       <  3:1544594/149‑1 (MQ=255)
                                gTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATaa                                                                                                                     <  3:946427/149‑1 (MQ=255)
                                   aCCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCa                                                                                                                  >  4:1086760/1‑149 (MQ=255)
                                     cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCAcc                                                                                                                >  3:260586/1‑149 (MQ=255)
                                      tttattCAGCAGCAGCTGCTTTACCGTGGTCTGTAAAGACGAGGCCTACATAGCCGGGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACcg                                                                                                               >  2:1545389/2‑149 (MQ=255)
                                      attattCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACcg                                                                                                               >  4:389117/1‑149 (MQ=255)
                                      attattCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACcg                                                                                                               >  1:439912/1‑149 (MQ=255)
                                       ttattCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACcgc                                                                                                              <  4:300567/149‑1 (MQ=255)
                                       ttattCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACcgc                                                                                                              <  1:565760/149‑1 (MQ=255)
                                          ttCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCa                                                                                                           >  3:1143771/1‑149 (MQ=255)
                                          ttCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGGGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCa                                                                                                           >  2:347927/1‑149 (MQ=255)
                                             agcagcagcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATcc                                                                                                        >  2:1552819/1‑149 (MQ=255)
                                              gcagcagcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCg                                                                                                       >  3:829874/1‑149 (MQ=255)
                                                   agcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCcaca                                                                                                  <  2:516695/149‑1 (MQ=255)
                                                    gcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCcacac                                                                                                 >  2:205373/1‑149 (MQ=255)
                                                       gATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCCTCCGCCACACaaa                                                                                              <  4:1233580/149‑1 (MQ=255)
                                                       gATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACaaa                                                                                              <  4:935056/149‑1 (MQ=255)
                                                           tACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCaa                                                                                          <  3:476461/149‑1 (MQ=255)
                                                            aCCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAAc                                                                                         >  4:1454382/1‑149 (MQ=255)
                                                            aCCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAAc                                                                                         <  4:471238/149‑1 (MQ=255)
                                                             ccGTGGTCTGGACAAACGTGGCCTACATAGCCGCGAGAAGAAAATATCCTGATTCGGTGAGTTTTCGCTAAAATAAGTGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACa                                                                                        <  2:212891/149‑1 (MQ=255)
                                                             ccGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACa                                                                                        <  1:248066/149‑1 (MQ=255)
                                                             ccGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACa                                                                                        <  1:872190/149‑1 (MQ=255)
                                                              cGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCg                                                                                                                                                <  2:258585/92‑1 (MQ=255)
                                                               gTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAgg                                                                                      >  4:1399894/1‑149 (MQ=255)
                                                                 ggTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCCCACAAAGCAACAGGaa                                                                                    <  4:1026595/149‑1 (MQ=255)
                                                                 ggTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGaa                                                                                    <  4:884030/149‑1 (MQ=255)
                                                                 ggTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGaa                                                                                    <  2:708416/149‑1 (MQ=255)
                                                                    cTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcat                                                                                 <  1:1552665/149‑1 (MQ=255)
                                                                     tGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGc                                                                                                      >  1:642532/1‑127 (MQ=255)
                                                                     tGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGc                                                                                                      <  2:642532/127‑1 (MQ=255)
                                                                       gAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGc                                                                                                                           <  1:279020/104‑1 (MQ=255)
                                                                       gAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGc                                                                                                                           >  2:279020/1‑104 (MQ=255)
                                                                       gAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatcat                                                                              <  2:130060/149‑1 (MQ=255)
                                                                       gAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatcat                                                                              >  3:891501/1‑149 (MQ=255)
                                                                       gAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatcat                                                                              >  4:801853/1‑149 (MQ=255)
                                                                        aaaaaCGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATg                                                                             >  3:1641741/1‑149 (MQ=255)
                                                                         aaaaCGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGa                                                                            <  4:1565082/149‑1 (MQ=255)
                                                                          aaaCGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAg                                                                           >  1:943284/1‑149 (MQ=255)
                                                                                 gCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAAt                                                                    <  1:1352045/149‑1 (MQ=255)
                                                                                   cTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGa                                                                  >  3:678751/1‑149 (MQ=255)
                                                                                       aTAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACt                                                              >  2:949740/1‑149 (MQ=255)
                                                                                         aGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTAt                                                            >  4:1546798/1‑149 (MQ=255)
                                                                                         aGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTAt                                                            >  4:1344860/1‑149 (MQ=255)
                                                                                         aGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTAt                                                            >  3:789051/1‑149 (MQ=255)
                                                                                          gCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCaa                                                                                          <  4:413727/118‑1 (MQ=255)
                                                                                          gCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATc                                                           <  3:997131/149‑1 (MQ=255)
                                                                                          gCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATc                                                           >  1:1041232/1‑149 (MQ=255)
                                                                                          gCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATc                                                           >  2:1752912/1‑149 (MQ=255)
                                                                                               gagaAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGc                                                      >  1:1378795/1‑149 (MQ=255)
                                                                                                  aagaaAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGaca                                                                <  3:1328577/136‑1 (MQ=255)
                                                                                                  aagaaAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCa                                                   >  3:514462/1‑149 (MQ=255)
                                                                                                     aaaaaaTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACaaa                                                                                              <  2:675431/103‑1 (MQ=255)
                                                                                                     aaaaaaTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACaaa                                                                                              >  1:675431/1‑103 (MQ=255)
                                                                                                     aaaaaaTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGc                                                >  2:885727/1‑149 (MQ=255)
                                                                                                      aaaaaTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGcc                                               <  3:1454382/149‑1 (MQ=255)
                                                                                                      aaaaaTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAACTGACCGCCcttattt                                                                                                                                       >  2:929145/1‑60 (MQ=25)
                                                                                                      aaaaaTCCTCATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAACTGACCGCCcttattt                                                                                                                                       <  1:929145/61‑2 (MQ=21)
                                                                                                       aaaaTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATaa                                                                     <  1:131105/126‑1 (MQ=255)
                                                                                                              tGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTa                                                        >  4:660314/1‑132 (MQ=255)
                                                                                                                    ggTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACg                                 >  2:1678082/1‑149 (MQ=255)
                                                                                                                     gTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGt                                <  2:1411052/149‑1 (MQ=255)
                                                                                                                              cGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGtt                       <  4:514462/149‑1 (MQ=255)
                                                                                                                              cGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGtt                       <  4:1641741/149‑1 (MQ=255)
                                                                                                                                cTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTgg                     >  1:1064626/1‑149 (MQ=255)
                                                                                                                                    aaTAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATc                 <  1:1608990/149‑1 (MQ=255)
                                                                                                                                      aaaaggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGt                                <  3:6511/128‑1 (MQ=255)
                                                                                                                                        aaggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTgc             >  2:1338131/3‑149 (MQ=255)
                                                                                                                                        aGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTgc             >  1:1239844/1‑149 (MQ=255)
                                                                                                                                         gggCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTgcg            >  4:21088/1‑149 (MQ=255)
                                                                                                                                         gggCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCATTg                      >  4:1093791/1‑139 (MQ=255)
                                                                                                                                         gcgCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATAGTAGCCCACGCCACGCCTCCCGGACGTGGCGGCGTTGGCATCCTgcg            >  1:1722916/3‑149 (MQ=255)
                                                                                                                                            cGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCAt         >  3:1230394/1‑149 (MQ=255)
                                                                                                                                                cAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTcc     <  3:38810/149‑1 (MQ=255)
                                                                                                                                                   ttGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTCCGGc  <  4:1535300/149‑1 (MQ=255)
                                                                                                                                                   ttGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTCCGGc  >  3:812506/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTCCGGC  >  NC_000913/3886610‑3886905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: