Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 378,398 A→G 5.6% intergenic (‑30/+64) frmB ← / ← frmA S‑formylglutathione hydrolase/alcohol dehydrogenase class III; glutathione‑dependent formaldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913378,3980AG 5.6% 601.7 / 14.4 188intergenic (‑30/+64)frmB/frmAS‑formylglutathione hydrolase/alcohol dehydrogenase class III; glutathione‑dependent formaldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (79/98);  new base G (6/5);  total (85/103)
Fisher's exact test for biased strand distribution p-value = 5.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

TTCGCGGCTTTTGGTGGGAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATG  >  NC_000913/378250‑378545
                                                                                                                                                    |                                                                                                                                                   
ttCGCGGCTTTTGGTGGGAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGa                                                                                                                                                     >  3:908241/1‑149 (MQ=255)
     ggCTTTTGGTGGGAGATAGACGCCGACATTCATTTCACATTTCAGAGATTGGGACTAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCt                                                                                                                                                <  3:1486007/149‑1 (MQ=255)
        ttttGGTGGGAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGcc                                                                                                                                             >  2:573879/1‑149 (MQ=255)
             gtggGAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGGCAGCCGTCCggtg                                                                                                                                        <  2:1280893/149‑3 (MQ=255)
              tggGAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGga                                                                                                                                       <  1:853041/149‑1 (MQ=255)
               gggAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGgag                                                                                                                                      >  2:1718231/1‑149 (MQ=255)
               gggAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGgag                                                                                                                                      <  2:647987/149‑1 (MQ=255)
                ggAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGgaga                                                                                                                                     <  3:637338/149‑1 (MQ=255)
                   gaTAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTa                                                                                                                                  >  3:1112937/1‑149 (MQ=255)
                     tAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTAtt                                                                                                                                <  3:79757/149‑1 (MQ=255)
                            cGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAAcc                                                                                                                         <  3:1348146/149‑1 (MQ=255)
                             gACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAAccc                                                                                                                        >  1:1018676/1‑149 (MQ=255)
                                attcattTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCggg                                                                                                                                         <  2:1499127/129‑1 (MQ=255)
                                attcattTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCg                                                                                                                     >  3:466108/1‑149 (MQ=255)
                                 ttcattTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCggg                                                                                                                                         >  4:940291/1‑128 (MQ=255)
                                 ttcattTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCggg                                                                                                                                         <  3:940291/128‑1 (MQ=255)
                                 ttcattTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGc                                                                                                                    <  4:1112937/149‑1 (MQ=255)
                                    atttcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGGCAGCCGTCCgggg                                                                                                                                        >  1:29587/1‑126 (MQ=255)
                                     tttcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGGCAGCCGTCCggggga                                                                                                                                      <  1:282675/127‑3 (MQ=255)
                                     tttcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGGCAGCCGTCCggggga                                                                                                                                      >  2:282675/1‑125 (MQ=255)
                                     tttcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGa                                                                                                                >  3:549726/1‑149 (MQ=255)
                                      ttcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGaa                                                                                                               <  3:1476121/149‑1 (MQ=255)
                                       tcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGGCAGCCGTCCgggg                                                                                                                                        >  4:1450864/1‑123 (MQ=255)
                                       tcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAg                                                                                                              <  2:1458923/149‑1 (MQ=255)
                                        cacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGaa                                                                                                               >  1:1584275/1‑147 (MQ=255)
                                        cacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTCACATGTTTTTCAATGAGTTCCATAAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGaa                                                                                                               <  2:1584275/147‑1 (MQ=255)
                                            tttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGAc                                                                                                         >  1:531712/1‑149 (MQ=255)
                                             ttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGTACg                                                                                                        <  2:1018676/149‑1 (MQ=255)
                                              tcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACgg                                                                                                       >  3:1001410/1‑149 (MQ=255)
                                               caGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACggg                                                                                                      <  4:454274/149‑1 (MQ=255)
                                                    aTTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTaaa                                                                                                 <  4:1526204/149‑1 (MQ=255)
                                                       gggAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACgggg                                                                                                     >  2:1622599/1‑142 (MQ=255)
                                                           ataatGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCgg                                                                                          >  4:246423/1‑149 (MQ=255)
                                                                gCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAAt                                                                                     <  4:1181914/149‑1 (MQ=255)
                                                                  cGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCa                                                                                   >  4:369991/1‑149 (MQ=255)
                                                                  cGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCa                                                                                   <  4:1613148/149‑1 (MQ=255)
                                                                    aTACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGt                                                                                 <  1:476964/149‑1 (MQ=255)
                                                                      aCATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTaa                                                                               <  2:579200/149‑1 (MQ=255)
                                                                         tatTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGc                                                                                            <  4:502344/133‑1 (MQ=255)
                                                                          atTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTTAACCTGCGGGAAATCAGTATCGaa                                                                           <  1:1491306/149‑1 (MQ=255)
                                                                           ttttGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAAt                                                                          >  2:79944/1‑149 (MQ=255)
                                                                            tttGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAAtt                                                                         <  3:369991/149‑1 (MQ=255)
                                                                             ttGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTa                                                                        <  4:1259665/149‑1 (MQ=255)
                                                                             ttGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTa                                                                        <  3:1284783/149‑1 (MQ=255)
                                                                                 cAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGt                                                                    <  2:494727/149‑1 (MQ=255)
                                                                                  aGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTAt                                                                                                                                 >  1:1008167/1‑87 (MQ=255)
                                                                                  aGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCg                                                                                           >  1:1467820/1‑125 (MQ=255)
                                                                                   gccgccAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTc                                                                  >  3:776299/1‑149 (MQ=255)
                                                                                      gccAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGaa                                                               <  2:213134/149‑1 (MQ=255)
                                                                                      gccAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGaa                                                               <  2:1235589/149‑1 (MQ=255)
                                                                                       ccAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCATTAACGAATTACGGTTCGAAt                                                              <  2:114015/149‑1 (MQ=255)
                                                                                       ccAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCCCAGAGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAAt                                                              <  4:1439008/149‑1 (MQ=255)
                                                                                        cAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATg                                                             >  3:791155/1‑149 (MQ=255)
                                                                                         aaaGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATgg                                                            >  2:484014/1‑149 (MQ=255)
                                                                                              tGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCgg                                                                                          <  1:655899/114‑1 (MQ=255)
                                                                                                aCATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGcc                                                     >  4:1260899/1‑149 (MQ=255)
                                                                                                          cAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATc                                           <  4:783541/149‑1 (MQ=255)
                                                                                                          cAATGAGTTCCATCAGTCATTATCTCAGCGTTAGTCACCCGGACGGCTGCCGGGGGAGAGTATTAAGAACCCCCGCCTGAAGAGGACGGGGGAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTTCCTTCATGCATc                                           >  4:1317733/1‑149 (MQ=255)
                                                                                                            aTGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACgggg                                                                                                     <  2:1121985/89‑1 (MQ=255)
                                                                                                            aTGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACgggg                                                                                                     >  1:1121985/1‑89 (MQ=255)
                                                                                                             tGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAgg                                        <  1:1718231/149‑1 (MQ=255)
                                                                                                                  tCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGaa                                   <  3:1449993/149‑1 (MQ=255)
                                                                                                                  tCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGaa                                   <  3:231750/149‑1 (MQ=255)
                                                                                                                  tCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGaa                                   <  4:1398636/149‑1 (MQ=255)
                                                                                                                     aTCAGTCATTATCTCAGGGTTAGTCCCCCGGACGGCTGCGCGGGGAGAGTATTAAGAACCCGCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGc                                <  4:974889/149‑1 (MQ=255)
                                                                                                                     aTCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCg                                                                                                                     <  3:1291617/64‑1 (MQ=255)
                                                                                                                     aTCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGc                                <  2:1385975/149‑1 (MQ=255)
                                                                                                                        aGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTc                             <  1:573879/149‑1 (MQ=255)
                                                                                                                         gTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCa                            >  3:170255/1‑149 (MQ=255)
                                                                                                                           cATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCAtt                          >  1:1526984/1‑149 (MQ=255)
                                                                                                                            aTTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTa                         >  3:768881/1‑149 (MQ=255)
                                                                                                                             ttATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGaa                                                                                                                           <  1:1652558/50‑1 (MQ=255)
                                                                                                                              tATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAAt                       <  3:975146/149‑1 (MQ=255)
                                                                                                                               aTCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAAtt                      <  4:211333/149‑1 (MQ=255)
                                                                                                                                  tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCa                                                                                   >  2:608156/1‑85 (MQ=255)
                                                                                                                                  tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCa                                                                                   <  1:608156/85‑1 (MQ=255)
                                                                                                                                  tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGt                                                                    <  2:1077489/100‑1 (MQ=255)
                                                                                                                                  tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGt                                                                    >  1:1077489/1‑100 (MQ=255)
                                                                                                                                  tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGc                                >  1:193115/1‑136 (MQ=255)
                                                                                                                                  tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCa                   >  3:1456928/1‑149 (MQ=255)
                                                                                                                                  tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCa                   >  2:1654405/1‑149 (MQ=255)
                                                                                                                                  tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCa                   >  2:1548031/1‑149 (MQ=255)
                                                                                                                                   cAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCAt                  <  4:171872/149‑1 (MQ=255)
                                                                                                                                     gggTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGAttt                                                        <  3:663579/109‑1 (MQ=255)
                                                                                                                                     gggTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGAttt                                                        >  4:663579/1‑109 (MQ=255)
                                                                                                                                       gTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAAt                                                                                     >  2:343394/1‑78 (MQ=255)
                                                                                                                                       gTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAAt                                                                                     <  1:343394/78‑1 (MQ=255)
                                                                                                                                       gTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAg              <  1:67075/149‑1 (MQ=255)
                                                                                                                                            tCCCCCGGACGGCTGCCCGGGGCGTGTATTAAGAACCTCCGCCTGAAGTGGACGGGGTAAACGTGCGGGCAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGCCATTAATTTCATCCAGGCTCa         >  2:65881/1‑149 (MQ=255)
                                                                                                                                            tCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCa         >  3:1574776/1‑149 (MQ=255)
                                                                                                                                             cccccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCAt        <  1:79944/149‑1 (MQ=255)
                                                                                                                                             cccccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCAt        >  1:1308908/1‑149 (MQ=255)
                                                                                                                                             cccccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCAt        <  2:1407874/149‑1 (MQ=255)
                                                                                                                                              ccccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGaa                                   <  1:967587/121‑1 (MQ=255)
                                                                                                                                              ccccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGaa                                   >  2:967587/1‑121 (MQ=255)
                                                                                                                                              ccccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATg       <  1:1654405/149‑1 (MQ=255)
                                                                                                                                              ccccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATg       <  3:258278/149‑1 (MQ=255)
                                                                                                                                              ccccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATg       <  4:549726/149‑1 (MQ=255)
                                                                                                                                               cccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGAGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATgg      >  3:33189/1‑149 (MQ=255)
                                                                                                                                                 cGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTa    <  3:181125/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCGCGGCTTTTGGTGGGAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATG  >  NC_000913/378250‑378545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: