Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 378,405 C→T 5.9% intergenic (‑37/+57) frmB ← / ← frmA S‑formylglutathione hydrolase/alcohol dehydrogenase class III; glutathione‑dependent formaldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913378,4050CT 5.9% 592.1 / 15.5 186intergenic (‑37/+57)frmB/frmAS‑formylglutathione hydrolase/alcohol dehydrogenase class III; glutathione‑dependent formaldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (79/96);  new base T (6/5);  total (85/101)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 187 total aligned reads displayed.

TTTTGGTGGGAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATGCGTGAC  >  NC_000913/378258‑378551
                                                                                                                                                   |                                                                                                                                                  
     gtggGAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGGCAGCCGTCCggtg                                                                                                                                              <  2:1280893/149‑3 (MQ=255)
      tggGAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGga                                                                                                                                             <  1:853041/149‑1 (MQ=255)
        ggAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGgaga                                                                                                                                           <  3:637338/149‑1 (MQ=255)
         gagaTAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGagag                                                                                                                                          >  2:642265/1‑149 (MQ=255)
         gagaTAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGagag                                                                                                                                          <  1:416118/149‑1 (MQ=255)
           gaTAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTa                                                                                                                                        >  3:1112937/1‑149 (MQ=255)
             tAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTAtt                                                                                                                                      <  3:79757/149‑1 (MQ=255)
                     gACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAAccc                                                                                                                              >  1:1018676/1‑149 (MQ=255)
                        attcattTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCg                                                                                                                           >  3:466108/1‑149 (MQ=255)
                        attcattTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCg                                                                                                                           >  3:1297626/1‑149 (MQ=255)
                         ttcattTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCggg                                                                                                                                               <  3:940291/128‑1 (MQ=255)
                         ttcattTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCggg                                                                                                                                               >  4:940291/1‑128 (MQ=255)
                          tcattTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGcc                                                                                                                         <  3:1064523/149‑1 (MQ=255)
                            atttcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGGCAGCCGTCCgggg                                                                                                                                              >  1:29587/1‑126 (MQ=255)
                            atttcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGGCAGCCGTCCgggg                                                                                                                                              <  2:29587/126‑1 (MQ=255)
                             tttcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGGCAGCCGTCCggggga                                                                                                                                            <  1:282675/127‑3 (MQ=255)
                             tttcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGa                                                                                                                      >  3:549726/1‑149 (MQ=255)
                               tcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGGCAGCCGTCCgggg                                                                                                                                              <  3:1450864/123‑1 (MQ=255)
                               tcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGGCAGCCGTCCgggg                                                                                                                                              >  4:1450864/1‑123 (MQ=255)
                                       caGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACggg                                                                                                            <  3:1438527/149‑1 (MQ=255)
                                       caGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACggg                                                                                                            <  4:454274/149‑1 (MQ=255)
                                        ggTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACgggg                                                                                                           <  2:599284/148‑1 (MQ=255)
                                            aTTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTaaa                                                                                                       <  4:1526204/149‑1 (MQ=255)
                                             ttGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAAc                                                                                                      <  2:69558/149‑1 (MQ=255)
                                               gggAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACgggg                                                                                                           <  1:1622599/142‑1 (MQ=255)
                                               gggAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACgggg                                                                                                           >  2:1622599/1‑142 (MQ=255)
                                                 gAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGc                                                                                                  <  2:1398461/149‑1 (MQ=255)
                                                          cGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCa                                                                                         <  4:1613148/149‑1 (MQ=255)
                                                            aTACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGt                                                                                       >  2:476964/1‑149 (MQ=255)
                                                            aTACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGt                                                                                       >  2:1557471/1‑149 (MQ=255)
                                                              aCATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTaa                                                                                     <  2:579200/149‑1 (MQ=255)
                                                                atatTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACg                                                                                   >  2:1445097/1‑149 (MQ=255)
                                                                 tatTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGc                                                                                                  <  4:502344/133‑1 (MQ=255)
                                                                  atTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTTAACCTGCGGGAAATCAGTATCGaa                                                                                 <  1:1491306/149‑1 (MQ=255)
                                                                    tttGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAAtt                                                                               <  3:369991/149‑1 (MQ=255)
                                                                     ttGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTa                                                                              <  3:1284783/149‑1 (MQ=255)
                                                                         cAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGt                                                                          >  2:857849/1‑149 (MQ=255)
                                                                          aGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTAt                                                                                                                                       <  2:1008167/87‑1 (MQ=255)
                                                                          aGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTAt                                                                                                                                       >  1:1008167/1‑87 (MQ=255)
                                                                          aGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCg                                                                                                 >  1:1467820/1‑125 (MQ=255)
                                                                          aGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCg                                                                                                 <  2:1467820/125‑1 (MQ=255)
                                                                           gccgccAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTc                                                                        >  3:776299/1‑149 (MQ=255)
                                                                              gccAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGaa                                                                     <  2:1235589/149‑1 (MQ=255)
                                                                               ccAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCATTAACGAATTACGGTTCGAAt                                                                    <  2:114015/149‑1 (MQ=255)
                                                                               ccAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCCCAGAGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAAt                                                                    <  4:1439008/149‑1 (MQ=255)
                                                                                cAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATg                                                                   >  3:791155/1‑149 (MQ=255)
                                                                                   aGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGAt                                                                <  2:1310053/149‑1 (MQ=255)
                                                                                      tGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCgg                                                                                                >  2:655899/1‑114 (MQ=255)
                                                                                      tGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCgg                                                                                                <  1:655899/114‑1 (MQ=255)
                                                                                       gACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGc                                                            <  4:1236952/149‑1 (MQ=255)
                                                                                        aCATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGcc                                                           >  2:1052297/1‑149 (MQ=255)
                                                                                        aCATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGcc                                                           >  4:1260899/1‑149 (MQ=255)
                                                                                          aTGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCtt                                                         >  4:449102/1‑149 (MQ=255)
                                                                                            gTTTTTCAATGAGATCCATCAGTCATTAGCTCAGGGTTAAGCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCttta                                                       <  1:1527025/149‑3 (MQ=255)
                                                                                             tttttCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGTAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCAt                                                      <  2:541069/149‑1 (MQ=255)
                                                                                             tttttCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCAt                                                      >  1:541069/1‑149 (MQ=255)
                                                                                                  cAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATc                                                 <  4:783541/149‑1 (MQ=255)
                                                                                                  cAATGAGTTCCATCAGTCATTATCTCAGCGTTAGTCACCCGGACGGCTGCCGGGGGAGAGTATTAAGAACCCCCGCCTGAAGAGGACGGGGGAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTTCCTTCATGCATc                                                 >  4:1317733/1‑149 (MQ=255)
                                                                                                    aTGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACgggg                                                                                                           >  1:1121985/1‑89 (MQ=255)
                                                                                                    aTGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAg                                               >  4:258278/1‑149 (MQ=255)
                                                                                                    aTGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAg                                               >  3:1467581/1‑149 (MQ=255)
                                                                                                     tGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAgg                                              <  1:1718231/149‑1 (MQ=255)
                                                                                                          tCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGaa                                         <  3:690697/149‑1 (MQ=255)
                                                                                                          tCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGaa                                         >  2:111056/1‑149 (MQ=255)
                                                                                                          tCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGaa                                         <  4:1398636/149‑1 (MQ=255)
                                                                                                            cATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACggg                                                                                                            <  3:1586951/80‑1 (MQ=255)
                                                                                                             aTCAGTCATTATCTCAGGGTTAGTCCCCCGGACGGCTGCGCGGGGAGAGTATTAAGAACCCGCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGc                                      <  4:974889/149‑1 (MQ=255)
                                                                                                                 gTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCa                                  <  2:994677/149‑1 (MQ=255)
                                                                                                                 gTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCa                                  >  3:170255/1‑149 (MQ=255)
                                                                                                                 gTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGATAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCa                                  >  4:1128909/1‑149 (MQ=255)
                                                                                                                  tCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCAt                                 >  2:1546203/1‑149 (MQ=255)
                                                                                                                  tCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCAt                                 >  2:171201/1‑149 (MQ=255)
                                                                                                                   cATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCAtt                                >  1:1526984/1‑149 (MQ=255)
                                                                                                                      tATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAAt                             <  3:975146/149‑1 (MQ=255)
                                                                                                                       aTCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAAtt                            <  4:1041213/149‑1 (MQ=255)
                                                                                                                       aTCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAAtt                            <  4:211333/149‑1 (MQ=255)
                                                                                                                          tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCa                                                                                         <  1:608156/85‑1 (MQ=255)
                                                                                                                          tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGt                                                                          >  1:1077489/1‑100 (MQ=255)
                                                                                                                          tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGc                                      <  2:193115/136‑1 (MQ=255)
                                                                                                                          tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGc                                      >  1:193115/1‑136 (MQ=255)
                                                                                                                          tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCa                         >  2:1654405/1‑149 (MQ=255)
                                                                                                                           cAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCAt                        <  4:171872/149‑1 (MQ=255)
                                                                                                                             gggTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGAttt                                                              >  4:663579/1‑109 (MQ=255)
                                                                                                                               gTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAAt                                                                                           <  1:343394/78‑1 (MQ=255)
                                                                                                                               gTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAg                    <  1:67075/149‑1 (MQ=255)
                                                                                                                                     tccccGGGCAGCCGTCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTACTTTCATCCAGGCTCAt              >  3:66076/2‑149 (MQ=255)
                                                                                                                                     cccccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCAt              <  1:79944/149‑1 (MQ=255)
                                                                                                                                     cccccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCAt              >  1:1308908/1‑149 (MQ=255)
                                                                                                                                      ccccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATg             <  1:1654405/149‑1 (MQ=255)
                                                                                                                                      ccccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATg             <  4:549726/149‑1 (MQ=255)
                                                                                                                                      ccccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATg             <  3:258278/149‑1 (MQ=255)
                                                                                                                                      ccccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATg             >  3:15260/1‑149 (MQ=255)
                                                                                                                                       cccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGtt                                                                         >  2:487674/1‑88 (MQ=255)
                                                                                                                                       cccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGtt                                                                         <  1:487674/88‑1 (MQ=255)
                                                                                                                                       cccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGAGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATgg            >  3:33189/1‑149 (MQ=255)
                                                                                                                                        ccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGt           >  2:216352/1‑149 (MQ=255)
                                                                                                                                         cGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTa          <  3:181125/149‑1 (MQ=255)
                                                                                                                                           gACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATg        >  1:906137/1‑149 (MQ=255)
                                                                                                                                              ggCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATGCGt     <  4:1456434/149‑1 (MQ=255)
                                                                                                                                                 tGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATGCGTGAc  >  4:1590784/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTTGGTGGGAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATGCGTGAC  >  NC_000913/378258‑378551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: