Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,376,819 A→T 6.7% intergenic (+120/‑56) ecnB → / → sugE entericidin B membrane lipoprotein/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,376,8190AT 6.7% 751.9 / 33.6 224intergenic (+120/‑56)ecnB/sugEentericidin B membrane lipoprotein/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (103/105);  new base T (9/6);  total (113/111)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.00e-01

Only 100 of 236 total aligned reads displayed.

CTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGAC  >  NC_000913/4376671‑4376963
                                                                                                                                                    |                                                                                                                                                
cTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGATAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGga                                                                                                                                                  <  1:1576353/149‑1 (MQ=255)
    cgcAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGgacgac                                                                                                                                              <  3:682374/149‑1 (MQ=255)
       agcaACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGgacgac                                                                                                                                              <  1:1458682/146‑1 (MQ=255)
          aaCGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCaa                                                                                                                                        >  3:1509999/1‑149 (MQ=255)
           aCGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGGCGACCTGCaaa                                                                                                                                       >  1:1588840/1‑149 (MQ=255)
             gAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACg                                                                                                                                     >  4:1486598/1‑149 (MQ=255)
                  cgcAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCctct                                                                                                                                <  3:1585718/149‑1 (MQ=255)
                   gcagcaATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCtt                                                                                                                               <  2:1619304/149‑1 (MQ=255)
                     agcaataagcaataACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCtttt                                                                                                                             <  2:1098580/149‑1 (MQ=255)
                     agcaataagcaataACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCtttt                                                                                                                             <  4:221980/149‑1 (MQ=255)
                        aataagcaataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCAc                                                                                                                          >  3:502610/1‑149 (MQ=255)
                         ataagcaataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCAcc                                                                                                                         >  2:849796/1‑149 (MQ=255)
                         ataagcaataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCAcc                                                                                                                         >  1:992901/1‑149 (MQ=255)
                               aataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGc                                                                                                                                          >  2:1492941/1‑126 (MQ=255)
                               aataaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGAc                                                                                                                   >  3:31470/1‑149 (MQ=255)
                                  aaCGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGc                                                                                                                                    >  4:1265107/1‑129 (MQ=255)
                                     ggTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGcccc                                                                                                             >  2:512985/1‑149 (MQ=255)
                                      gTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGga                                                                                                                                                  >  1:1166871/1‑111 (MQ=255)
                                          gACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATtc                                                                                                        >  1:1050003/1‑149 (MQ=255)
                                          gACAGCTGTGTCGTGCCGTATGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATtc                                                                                                        >  3:1425947/1‑149 (MQ=255)
                                            cAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGga                                                                                                                                                  <  2:93776/105‑1 (MQ=255)
                                              gCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCg                                                                                                    <  4:1229102/149‑1 (MQ=255)
                                              gCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCg                                                                                                    <  2:1216173/149‑1 (MQ=255)
                                              gCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCg                                                                                                    <  1:514205/149‑1 (MQ=255)
                                                tgtgTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGa                                                                                                  <  2:898422/149‑1 (MQ=255)
                                                      gTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGa                                                                                            <  2:1594693/149‑1 (MQ=255)
                                                       tGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGgacgac                                                                                                                                              >  4:302400/1‑98 (MQ=255)
                                                       tGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCg                                                                                                    <  2:1076199/140‑1 (MQ=255)
                                                       tGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGc                                                                                                <  3:675325/144‑1 (MQ=255)
                                                       tGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGc                                                                                                >  4:675325/1‑144 (MQ=255)
                                                       tGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGat                                                                                           <  2:633711/149‑1 (MQ=255)
                                                           gtttgattTTTCTGCGATAGTCACAAGGTTATTAGTTAAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTGGCAGGACGACCGGCAAACGCCTCTTTTCACTGGGGACGGCCCCAATTCTCGGAAGCCTGATATGt                                                                                       >  4:450164/1‑149 (MQ=255)
                                                            tttgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTc                                                                                      >  1:131801/1‑149 (MQ=255)
                                                              tgtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCt                                                                                    <  1:1110091/149‑1 (MQ=255)
                                                               gtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTg                                                                                   <  3:1486598/149‑1 (MQ=255)
                                                               gtttTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTg                                                                                   >  1:848806/1‑149 (MQ=255)
                                                                ttttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTgg                                                                                  >  3:1369935/1‑149 (MQ=255)
                                                                 tttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGa                                                                                 <  1:203332/149‑1 (MQ=255)
                                                                 tttttCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGa                                                                                 <  4:522758/149‑1 (MQ=255)
                                                                      cTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATc                                                                            >  4:1526270/1‑149 (MQ=255)
                                                                           aTAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCTTGGATTATCTTAGt                                                                       >  3:659000/1‑149 (MQ=255)
                                                                           aTAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGt                                                                       <  4:1369935/149‑1 (MQ=255)
                                                                           aTAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGt                                                                       <  3:466467/149‑1 (MQ=255)
                                                                                 acaAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGa                                                                                                                    >  1:775182/1‑98 (MQ=255)
                                                                                 acaAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTg                                                                                   >  1:651757/1‑131 (MQ=255)
                                                                                  caAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCggg                                                                                                                      >  3:746947/1‑95 (MQ=255)
                                                                                  caAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCggg                                                                                                                      <  4:746947/95‑1 (MQ=255)
                                                                                  caAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCt                                                                <  2:474311/149‑1 (MQ=255)
                                                                                    aaGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTgg                                                              <  3:1066611/149‑1 (MQ=255)
                                                                                          aTAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTctgct                                                        <  1:608514/149‑1 (MQ=255)
                                                                                            aGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTgg                                                      <  4:236854/149‑1 (MQ=255)
                                                                                             gTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTg                                                                                             <  1:621469/109‑1 (MQ=255)
                                                                                             gTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGa                                                     >  4:1579654/1‑149 (MQ=255)
                                                                                             gTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGa                                                     >  2:1125630/1‑149 (MQ=255)
                                                                                              ttGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGaa                                                    >  1:283816/1‑149 (MQ=255)
                                                                                              ttGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGaa                                                    >  2:259806/1‑149 (MQ=255)
                                                                                              ttGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGaa                                                    >  2:329822/1‑149 (MQ=255)
                                                                                                gacaTTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGATGt                                                  <  3:1148070/146‑1 (MQ=255)
                                                                                                gAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGt                                                  >  2:244354/1‑149 (MQ=255)
                                                                                                 aaaTTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTg                                                 <  1:1070528/149‑1 (MQ=255)
                                                                                                 aaaTTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACAGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTg                                                 <  2:57689/149‑1 (MQ=255)
                                                                                                   aTTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGt                                               >  2:996840/1‑149 (MQ=255)
                                                                                                   aTTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGt                                               >  1:1269924/1‑149 (MQ=255)
                                                                                                     tCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTAt                                             <  4:616281/149‑1 (MQ=255)
                                                                                                       cccTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATgg                                           <  3:1089208/149‑1 (MQ=255)
                                                                                                       cccTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATgg                                           >  3:965660/1‑149 (MQ=255)
                                                                                                       cccTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATgg                                           >  1:961223/1‑149 (MQ=255)
                                                                                                       cccTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATgg                                           <  2:1269924/149‑1 (MQ=255)
                                                                                                            ccACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGt                                      <  1:476806/149‑1 (MQ=255)
                                                                                                                cTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGc                                  <  3:1031109/149‑1 (MQ=255)
                                                                                                                cTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGc                                  <  3:628292/149‑1 (MQ=255)
                                                                                                                 tGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGcc                                 >  1:499016/1‑149 (MQ=255)
                                                                                                                     aaaaTATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGaa                             >  3:333305/1‑149 (MQ=255)
                                                                                                                          atCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAAtata                        >  1:1696822/1‑149 (MQ=255)
                                                                                                                           tCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATAc                       >  2:1388038/1‑149 (MQ=255)
                                                                                                                           tCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATAc                       >  2:1516876/1‑149 (MQ=255)
                                                                                                                              gTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCa                    >  3:1298537/1‑149 (MQ=255)
                                                                                                                               ttCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCAc                   <  4:1298537/149‑1 (MQ=255)
                                                                                                                                  aaCCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGc                >  1:686807/1‑149 (MQ=255)
                                                                                                                                   aCCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCt               <  2:132874/149‑1 (MQ=255)
                                                                                                                                    ccATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCtt              >  4:1496947/1‑149 (MQ=255)
                                                                                                                                     cATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCttt             >  2:1165316/1‑149 (MQ=255)
                                                                                                                                      aTCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTa            <  2:68615/149‑1 (MQ=255)
                                                                                                                                      aTCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTa            >  1:1445920/1‑149 (MQ=255)
                                                                                                                                      aTCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTa            <  3:267023/149‑1 (MQ=255)
                                                                                                                                      aTCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTa            >  3:1700863/1‑149 (MQ=255)
                                                                                                                                       tCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAg           >  4:1649728/1‑149 (MQ=255)
                                                                                                                                        cAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGa                                                                                 <  3:1264881/78‑1 (MQ=255)
                                                                                                                                           gtttGCAGGTCGTCCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGt                                                  <  2:818708/105‑1 (MQ=255)
                                                                                                                                           gtttGCAGGTCGTCCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGt       >  2:704974/2‑149 (MQ=255)
                                                                                                                                           cTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGt       <  3:1200792/149‑1 (MQ=255)
                                                                                                                                            tttGCAGGTCGTCCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTg                                                 <  1:1541799/106‑1 (MQ=255)
                                                                                                                                            tttGCAGGACGAGCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGtt      >  4:1083601/1‑149 (MQ=255)
                                                                                                                                            tttGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGtt      <  3:614602/149‑1 (MQ=255)
                                                                                                                                            tttGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGtt      <  4:1389127/149‑1 (MQ=255)
                                                                                                                                            tttGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGtt      <  2:1370648/149‑1 (MQ=255)
                                                                                                                                            tttGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGtt      >  2:775401/1‑149 (MQ=255)
                                                                                                                                             ttGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCACCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGttt     <  2:1445920/149‑1 (MQ=255)
                                                                                                                                              tGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCa                                                                                                            >  4:1161297/1‑45 (MQ=255)
                                                                                                                                                 aGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTc         >  3:1559583/1‑141 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGCCACCTGGCAAAATATCCGTTCAACCATCAGCTTTGCAGGACGACCTGCAAACGCCTCTTTTCACCGGGGACGGCCCCAATTCTCCGGAGCCTGATATGTCCTGGATTATCTTAGTTATTGCTGGTCTGCTGGAAGTGGTATGGGCCGTTGGCCTGAAATATACCCACGGCTTTAGTCGTTTGAC  >  NC_000913/4376671‑4376963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: