Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,608,604 C→G 5.2% I140M (ATC→ATG rimI → ribosomal‑protein‑S18‑alanine N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,608,6040CG 5.2% 708.6 / 20.5 205I140M (ATC→ATGrimIribosomal‑protein‑S18‑alanine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (98/95);  new base G (3/8);  total (101/104)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 220 total aligned reads displayed.

TGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGC  >  NC_000913/4608459‑4608752
                                                                                                                                                 |                                                                                                                                                    
tGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCcatcatc                                                                                                                                                   >  3:559268/1‑149 (MQ=255)
tGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCcatcatc                                                                                                                                                   <  4:163786/149‑1 (MQ=255)
  gAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCcatcatcat                                                                                                                                                 >  2:31054/1‑149 (MQ=255)
  gAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCcatcatcat                                                                                                                                                 >  1:1356409/1‑149 (MQ=255)
   aaaaaCGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATg                                                                                                                                                >  4:68010/1‑149 (MQ=255)
    aaaaCGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATgg                                                                                                                                               >  4:288964/1‑149 (MQ=255)
     aaaCGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGc                                                                                                                                              <  1:333248/149‑1 (MQ=255)
     aaaCGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGc                                                                                                                                              >  3:8724/1‑149 (MQ=255)
       gcgcgGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATGATGATGGCGt                                                                                                                                            <  2:1371330/148‑1 (MQ=255)
       aCGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGt                                                                                                                                            <  3:334636/149‑1 (MQ=255)
        cgcgGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATTATCATGGCGtt                                                                                                                                           <  3:1006338/149‑1 (MQ=255)
         gcggcgTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGGGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTg                                                                                                                                          >  3:168229/1‑149 (MQ=255)
          cggcgTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGc                                                                                                                                         <  2:1455683/149‑1 (MQ=255)
           ggcgTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATGATGATGGCGtcttc                                                                                                                                        <  4:837466/149‑5 (MQ=255)
              gTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAAt                                                                                                                                     <  1:1661230/149‑1 (MQ=255)
                    acacTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCCCCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTAt                                                                                                                               <  3:1128450/149‑1 (MQ=255)
                      acTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGt                                                                                                                             >  4:1124295/1‑149 (MQ=255)
                          tGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATa                                                                                                                         <  1:653875/149‑1 (MQ=255)
                              tGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAg                                                                                                                     <  4:8724/149‑1 (MQ=255)
                              tGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAg                                                                                                                     >  4:688674/1‑149 (MQ=255)
                                 aaGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTg                                                                                                                  <  1:211738/149‑1 (MQ=255)
                                   gTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGa                                                                                                                >  2:901883/1‑149 (MQ=255)
                                    tCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTCCCCCACCCCGGACGGTAGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaa                                                                                                               <  1:1438215/149‑1 (MQ=255)
                                    tCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaa                                                                                                               <  2:757532/149‑1 (MQ=255)
                                      cGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaata                                                                                                             <  4:1543739/149‑1 (MQ=255)
                                        tGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaataat                                                                                                           >  1:276541/1‑149 (MQ=255)
                                           ttaaaaCGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGaa                                                                                                        <  2:209228/146‑1 (MQ=255)
                                           ttCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATGATGATGGCGtc                                                                                                                                           >  1:1756034/1‑113 (MQ=255)
                                           ttCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGaa                                                                                                        >  1:89808/1‑149 (MQ=255)
                                            tCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGAAATTACTACCCCACCACGGCCGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAg                                                                                                       <  3:1482278/149‑1 (MQ=255)
                                             cAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGt                                                                                                      >  2:588516/1‑149 (MQ=255)
                                             cAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGt                                                                                                      <  1:1548937/149‑1 (MQ=255)
                                              aaaCGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTgg                                                                                                                 >  3:1635653/1‑137 (MQ=255)
                                               aaCGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTgg                                                                                                    >  1:846548/1‑149 (MQ=255)
                                               aaCGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTgg                                                                                                    <  4:1570814/149‑1 (MQ=255)
                                                  gCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGAc                                                                                                 >  4:540564/1‑149 (MQ=255)
                                                           aTTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTc                                                                                        <  2:459931/149‑1 (MQ=255)
                                                           aTTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTc                                                                                        <  3:266150/149‑1 (MQ=255)
                                                           aTTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTc                                                                                        <  1:704216/149‑1 (MQ=255)
                                                            ttGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCt                                                                                       >  2:850453/1‑149 (MQ=255)
                                                              gCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCttt                                                                                     <  4:318293/149‑1 (MQ=255)
                                                              gCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCttt                                                                                     <  3:68010/149‑1 (MQ=255)
                                                               cccTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAg                                                                                                                                  >  2:1744092/1‑103 (MQ=255)
                                                               cccTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAg                                                                                                                                  <  1:1744092/103‑1 (MQ=255)
                                                                 cTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGAt                                                                                  >  1:352803/1‑149 (MQ=255)
                                                                      cGAGAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGa                                                                             <  1:588516/149‑1 (MQ=255)
                                                                       gAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGaa                                                                                                        >  2:1204236/1‑121 (MQ=255)
                                                                       gAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGAt                                                                            >  2:1001869/1‑149 (MQ=255)
                                                                        aaaGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATg                                                                           <  3:540564/149‑1 (MQ=255)
                                                                           gTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGaaa                                                                        <  3:1283750/149‑1 (MQ=255)
                                                                            tttAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAAc                                                                       >  3:810118/1‑149 (MQ=255)
                                                                             ttAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaataat                                                                                                           <  2:167367/112‑1 (MQ=255)
                                                                               aGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCt                                                                    >  2:1055047/1‑149 (MQ=255)
                                                                                 gCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGt                                                                  <  1:1055047/149‑1 (MQ=255)
                                                                                  cTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGtt                                                                 <  1:755475/149‑1 (MQ=255)
                                                                                   tttAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaataat                                                                                                           >  3:1308549/1‑106 (MQ=255)
                                                                                   tttAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGttt                                                                >  2:970753/1‑149 (MQ=255)
                                                                                      aaCGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTAcc                                                             <  3:778805/149‑1 (MQ=255)
                                                                                      aaCGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTAcc                                                             >  3:230877/1‑149 (MQ=255)
                                                                                       aCGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTTCCt                                                            >  1:455145/1‑149 (MQ=255)
                                                                                       aCGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTAcc                                                             >  1:1111393/1‑148 (MQ=255)
                                                                                         gAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCttt                                                          >  4:934313/1‑149 (MQ=255)
                                                                                              gacgaTTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTc                                                     >  4:773930/1‑149 (MQ=255)
                                                                                               acgaTTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCa                                                    >  4:655189/1‑149 (MQ=255)
                                                                                                   ttCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCa                                                >  2:1239383/1‑149 (MQ=255)
                                                                                                      gccgcAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCtt                                                                                      <  4:1388806/108‑1 (MQ=255)
                                                                                                       ccgcAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCgg                                            >  1:638078/1‑149 (MQ=255)
                                                                                                       ccgcAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGACGAAACGCTGTTTACCTTTg                                                         <  2:1434571/136‑1 (MQ=255)
                                                                                                       ccgcAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGACGAAACGCTGTTTACCTTTg                                                         >  1:1434571/1‑136 (MQ=255)
                                                                                                                ctacCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCg                                   >  4:809689/1‑149 (MQ=255)
                                                                                                                 tacCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTGGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCgg                                  >  2:1114868/1‑149 (MQ=255)
                                                                                                                  acCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGa                                 >  1:1172143/1‑149 (MQ=255)
                                                                                                                   ccccACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGAt                                <  2:622274/149‑1 (MQ=255)
                                                                                                                    cccACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATg                               >  1:1002432/1‑149 (MQ=255)
                                                                                                                    cccACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATg                               >  3:1078609/1‑149 (MQ=255)
                                                                                                                     ccaccaCGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGt                              >  3:1358393/1‑149 (MQ=255)
                                                                                                                      caccacGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGtt                             >  3:423534/1‑149 (MQ=255)
                                                                                                                      caccacGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGtt                             >  4:457485/1‑149 (MQ=255)
                                                                                                                       accacGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGttt                            <  1:851327/149‑1 (MQ=255)
                                                                                                                        ccacGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTc                           <  1:604549/149‑1 (MQ=255)
                                                                                                                         cacGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCt                          >  1:1101321/1‑149 (MQ=255)
                                                                                                                         cacGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCt                          <  2:346159/149‑1 (MQ=255)
                                                                                                                           cggacggTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTg                        >  1:1218231/1‑149 (MQ=255)
                                                                                                                            ggacggTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGa                       <  4:168229/149‑1 (MQ=255)
                                                                                                                                ggTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTAc                   >  4:1033141/1‑149 (MQ=255)
                                                                                                                                  tCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGATTATTAAAGGGGAATGGGTTTTCTTTGATGCCGATGAAAAGGCGTTTACCTTTGGCTCCTTTACCGGCCCGCAGCCGGTGGTTTTTTATTACCGc                >  2:871254/1‑149 (MQ=255)
                                                                                                                                   cgcgAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGCTGGAATAATGAAGTGGGACTGGATTTTCttt                                                                                     <  4:1060055/80‑1 (MQ=255)
                                                                                                                                     cgAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGt              >  1:1591726/1‑149 (MQ=255)
                                                                                                                                     cgAAGACGCCATCATCATAGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGt              >  4:1349784/1‑149 (MQ=255)
                                                                                                                                         gACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCAcc          >  3:1223702/1‑149 (MQ=255)
                                                                                                                                         aaCGCCATGATGATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCAcc          >  1:139434/2‑149 (MQ=255)
                                                                                                                                          aCGCCATGATGATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCAcc          <  2:1108895/148‑1 (MQ=255)
                                                                                                                                            gCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCtt                         <  4:255754/131‑1 (MQ=255)
                                                                                                                                            gCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCttt       >  3:376821/1‑149 (MQ=255)
                                                                                                                                            gCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCttt       >  2:1174924/1‑149 (MQ=255)
                                                                                                                                            gCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCttt       >  2:743273/1‑149 (MQ=255)
                                                                                                                                              catcatcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTAc     >  1:31445/1‑149 (MQ=255)
                                                                                                                                              catcatcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTAc     >  4:1188421/1‑149 (MQ=255)
                                                                                                                                               atcatcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTAcc    >  4:187700/1‑149 (MQ=255)
                                                                                                                                               atcatcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTAcc    <  3:1250996/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGC  >  NC_000913/4608459‑4608752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: