Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 448,966 T→C 5.1% H559R (CAC→CGC) ‡ cyoB ← cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913448,9660TC 5.1% 628.2 / 13.8 192H559R (CAC→CGC) ‡cyoBcytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base T (88/93);  new base C (3/7);  total (91/101)
Fisher's exact test for biased strand distribution p-value = 3.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

CCAGATCATGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAG  >  NC_000913/448818‑449114
                                                                                                                                                    |                                                                                                                                                    
ccAGATCATGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGGATCACGTTCGt                                                                                                                                                      <  3:978001/149‑1 (MQ=255)
ccAGATCATGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGt                                                                                                                                                      <  1:546747/149‑1 (MQ=255)
   gATCATGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGaa                                                                                                                                                   >  2:1539717/1‑149 (MQ=255)
    aTCATGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAAc                                                                                                                                                  <  4:559918/149‑1 (MQ=255)
       aTGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTg                                                                                                                                               >  1:1418379/1‑149 (MQ=255)
         ggCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCACGAACGTgat                                                                                                                                             <  4:1435649/149‑3 (MQ=255)
         ggCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCACGAACGTgat                                                                                                                                             <  3:241481/149‑3 (MQ=255)
         ggCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCACGAACGTgat                                                                                                                                             <  1:383661/149‑3 (MQ=255)
            gAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCa                                                                                                                                          >  4:364094/1‑149 (MQ=255)
            gAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCa                                                                                                                                          >  1:134156/1‑149 (MQ=255)
                  gAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACgg                                                                                                                                    >  2:1689673/1‑149 (MQ=255)
                  gAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACgg                                                                                                                                    <  3:943206/149‑1 (MQ=255)
                   aaGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGt                                                                                                                                                      <  2:676174/130‑1 (MQ=255)
                    aGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCa                                                                                                                                  >  2:888186/1‑149 (MQ=255)
                      aTGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCaaa                                                                                                                                <  2:373904/149‑1 (MQ=255)
                         gtggAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGtt                                                                                                                             <  1:746510/149‑1 (MQ=255)
                         gtggAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGtt                                                                                                                             <  3:593741/149‑1 (MQ=255)
                           ggAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTtat                                                                                                                           <  2:1744477/149‑1 (MQ=255)
                            gagaAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTtata                                                                                                                          <  3:1570310/149‑1 (MQ=255)
                            gagaAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTtata                                                                                                                          <  2:416519/149‑1 (MQ=255)
                              gaAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGa                                                                                                                        >  4:117949/1‑149 (MQ=255)
                               aaaGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGaa                                                                                                                       <  4:264128/149‑1 (MQ=255)
                                 aGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACg                                                                                                                     >  2:1008548/1‑149 (MQ=255)
                                   cTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGa                                                                                                                   <  2:1513861/149‑1 (MQ=255)
                                   cTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGa                                                                                                                   <  1:280041/149‑1 (MQ=255)
                                   cTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGa                                                                                                                   >  4:1717776/1‑149 (MQ=255)
                                   cTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGa                                                                                                                   <  1:1689673/149‑1 (MQ=255)
                                       aaTGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCg                                                                                                               <  3:655431/149‑1 (MQ=255)
                                        aTGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCgg                                                                                                              <  3:468152/149‑1 (MQ=255)
                                         tGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCgg                                                                                                              <  4:1237862/148‑1 (MQ=255)
                                         tGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCgg                                                                                                              >  3:1237862/1‑148 (MQ=255)
                                            cGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGaa                                                                                                          <  3:1395320/149‑1 (MQ=255)
                                             gATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTta                                                                                                                            <  2:392608/130‑1 (MQ=255)
                                             gATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAag                                                                                                         >  3:738124/1‑149 (MQ=255)
                                              aTACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAaga                                                                                                        <  2:1047318/149‑1 (MQ=255)
                                               tACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAagag                                                                                                       <  2:781849/149‑1 (MQ=255)
                                                  cTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAaga                                                                                                        >  2:1484192/1‑145 (MQ=255)
                                                  cTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGtt                                                                                                    >  1:1200651/1‑149 (MQ=255)
                                                  cTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGtt                                                                                                    >  3:184231/1‑149 (MQ=255)
                                                  cTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGtt                                                                                                    <  3:1635436/149‑1 (MQ=255)
                                                    gCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGATCGTGCGGCACTACGGCAAAGTTATAGAACGGCGGCGGGGAAGAGGTTGc                                                                                                  <  4:1066950/149‑1 (MQ=255)
                                                    gCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGc                                                                                                  <  2:1218477/149‑1 (MQ=255)
                                                      aCCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGccc                                                                                                >  4:580989/1‑149 (MQ=255)
                                                        cGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCAc                                                                                              >  2:193281/1‑149 (MQ=255)
                                                          cTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTc                                                                                            <  4:1131822/149‑1 (MQ=255)
                                                          cTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTc                                                                                            >  2:1620975/1‑149 (MQ=255)
                                                           tGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCaaa                                                                                                                                >  4:856645/1‑112 (MQ=255)
                                                                      atatGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGcc                                                                                >  3:1599138/1‑149 (MQ=255)
                                                                       tatGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCa                                                                               >  3:910505/1‑149 (MQ=255)
                                                                       tatGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCa                                                                               >  1:486002/1‑149 (MQ=255)
                                                                       tatGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCa                                                                               <  4:1404819/149‑1 (MQ=255)
                                                                        atGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCAc                                                                              >  4:167479/1‑149 (MQ=255)
                                                                           aaTTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCAcccc                                                                           >  1:675527/1‑149 (MQ=255)
                                                                            aTTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCa                                                                          >  4:1286/1‑149 (MQ=255)
                                                                             tttCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCAc                                                                         >  4:4519/1‑149 (MQ=255)
                                                                                 ttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGTAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGt                                                                     >  4:227981/1‑149 (MQ=255)
                                                                                             aGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGt                                                         >  2:564819/1‑149 (MQ=255)
                                                                                                     tataCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTc                                                 <  3:364094/149‑1 (MQ=255)
                                                                                                       taCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTg                                               >  4:876/1‑149 (MQ=255)
                                                                                                         cGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggt                                             <  4:1192197/149‑1 (MQ=255)
                                                                                                           cTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcg                                           >  1:927467/1‑149 (MQ=255)
                                                                                                            ttCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgc                                          <  4:1004196/149‑1 (MQ=255)
                                                                                                            ttCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgc                                          >  4:1468247/1‑149 (MQ=255)
                                                                                                             tCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcg                                         <  4:1144931/149‑1 (MQ=255)
                                                                                                               gCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggt                                       >  4:702376/1‑149 (MQ=255)
                                                                                                                  tttCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtcgc                                    >  3:302701/1‑149 (MQ=255)
                                                                                                                    tctcTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGa                                  <  4:895796/149‑1 (MQ=255)
                                                                                                                     ctctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGaa                                 <  4:302701/149‑1 (MQ=255)
                                                                                                                     ctctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGaa                                 <  2:486002/149‑1 (MQ=255)
                                                                                                                       ctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATa                               <  1:398782/149‑1 (MQ=255)
                                                                                                                        tttCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGcc                                                                                                 <  1:202800/82‑1 (MQ=255)
                                                                                                                        tttCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGcc                                                                                                 >  2:202800/1‑82 (MQ=255)
                                                                                                                        tttCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCa                                                                                          >  3:678922/1‑89 (MQ=255)
                                                                                                                        tttCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAg                              >  3:1404893/1‑149 (MQ=255)
                                                                                                                        tttCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAg                              >  4:475802/1‑149 (MQ=255)
                                                                                                                        tttCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAg                              >  2:661674/1‑149 (MQ=255)
                                                                                                                         ttCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGa                             >  3:265765/1‑149 (MQ=255)
                                                                                                                           cATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGaaa                           <  4:1563709/149‑1 (MQ=255)
                                                                                                                            aTTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGaa                                 <  2:980255/142‑1 (MQ=255)
                                                                                                                            aTTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGaaa                           <  3:1541239/148‑1 (MQ=255)
                                                                                                                            aTTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAAc                          <  1:1119684/149‑1 (MQ=255)
                                                                                                                             tttCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACg                         >  1:162647/1‑149 (MQ=255)
                                                                                                                             tttCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACg                         >  3:1037427/1‑149 (MQ=255)
                                                                                                                              ttCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGTTTCTATTCGCGACCGCGACCAGAACCGt                        <  4:150375/149‑1 (MQ=255)
                                                                                                                               tCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTa                       >  2:108723/1‑149 (MQ=255)
                                                                                                                                 ccAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACa                     >  1:386312/1‑149 (MQ=255)
                                                                                                                                  cAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCaaa                                                                                                                                >  4:664052/1‑41 (MQ=255)
                                                                                                                                    gAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCt                  <  2:1226003/149‑1 (MQ=255)
                                                                                                                                    gAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCt                  <  3:117949/149‑1 (MQ=255)
                                                                                                                                          aTCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATaa            <  2:59392/149‑1 (MQ=255)
                                                                                                                                          aTCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATaa            >  2:1296643/1‑149 (MQ=255)
                                                                                                                                           tCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAc           <  3:1497906/149‑1 (MQ=255)
                                                                                                                                           gcACGTTCACGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAc           >  1:15472/2‑149 (MQ=255)
                                                                                                                                             aCGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTAGCGAATAGAAACGTACATCTGAATAACGa         <  4:738124/149‑1 (MQ=255)
                                                                                                                                              cGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAg        <  3:1367408/149‑1 (MQ=255)
                                                                                                                                              cGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAg        <  1:1633995/149‑1 (MQ=255)
                                                                                                                                               gTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAgg       >  2:1693640/1‑149 (MQ=255)
                                                                                                                                                    tGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGa                >  4:627522/1‑135 (MQ=255)
                                                                                                                                                    tGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGa                <  3:627522/135‑1 (MQ=255)
                                                                                                                                                    tGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCagag  >  2:727088/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCAGATCATGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAG  >  NC_000913/448818‑449114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: