Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 475,971 C→G 9.4% intergenic (+20/+11) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913475,9710CG 9.4% 708.0 / 50.7 211intergenic (+20/+11)ybaY/ybaZouter membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
Reads supporting (aligned to +/- strand):  ref base C (98/93);  new base G (8/12);  total (106/105)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 219 total aligned reads displayed.

ATTGGTGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACCC  >  NC_000913/475825‑476118
                                                                                                                                                  |                                                                                                                                                   
aTTGGTGCCGGTACAGCAAACCGCCGTGTCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGccc                                                                                                                                                   >  1:1030193/1‑149 (MQ=255)
aTTGGTGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATAATAAGCGACGTTAACCATCTCCGccc                                                                                                                                                   >  1:1607696/1‑149 (MQ=255)
     tGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGgaga                                                                                                                                              <  2:1193527/149‑1 (MQ=255)
       ccGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAgg                                                                                                                                            <  4:635642/149‑1 (MQ=255)
       ccGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAgg                                                                                                                                            <  2:299510/149‑1 (MQ=255)
        cGGTAGAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAggg                                                                                                                                           <  3:247933/149‑1 (MQ=255)
        cGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAggg                                                                                                                                           <  2:654346/149‑1 (MQ=255)
         ggTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGt                                                                                                                                          >  3:100081/1‑149 (MQ=255)
         ggTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGt                                                                                                                                          <  3:1109079/149‑1 (MQ=255)
           tACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTta                                                                                                                                        <  4:1209957/149‑1 (MQ=255)
            aCAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtag                                                                                                                                       <  2:1676886/149‑1 (MQ=255)
                cAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTagtagt                                                                                                                                   >  2:620111/1‑149 (MQ=255)
                  aaCCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGtt                                                                                                                                         <  1:946721/141‑1 (MQ=255)
                  aaCCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTc                                                                                                                                 >  2:960877/1‑149 (MQ=255)
                  aaCCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTc                                                                                                                                 <  1:620111/149‑1 (MQ=255)
                       ccgTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCg                                                                                                                            <  3:1391595/149‑1 (MQ=255)
                             cGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACg                                                                                                                      <  2:1303049/149‑1 (MQ=255)
                             cGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACg                                                                                                                      >  4:1600332/1‑149 (MQ=255)
                              ggTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGc                                                                                                                     <  2:886674/149‑1 (MQ=255)
                                ttCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTg                                                                                                                   <  3:125237/149‑1 (MQ=255)
                                     gCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGt                                                                                                              >  4:949607/1‑149 (MQ=255)
                                       cAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAGGGTtaacgt                                                                                                                                    >  4:1481859/1‑121 (MQ=255)
                                       cAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAGGGTtaacgt                                                                                                                                    <  3:1481859/125‑5 (MQ=255)
                                       cAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCg                                                                                                            >  4:489398/1‑149 (MQ=255)
                                           ggTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGAttt                                                                                                        <  4:827671/149‑1 (MQ=255)
                                             tGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGc                                                                                                      <  4:1174337/149‑1 (MQ=255)
                                              ggCGCAACGACTACCGTACCTTCGACTTCACCAACTCCGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGcc                                                                                                     <  4:872308/149‑1 (MQ=255)
                                                 gcAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTTCTACGCAATATTAAGCGACGTTAACCCTCGCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGGCCGc                                                                                                  >  2:212748/1‑149 (MQ=255)
                                                 gcAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGc                                                                                                  >  1:86163/1‑149 (MQ=255)
                                                     cGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTcc                                                                                              <  1:212748/149‑1 (MQ=255)
                                                        cTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGa                                                                                           <  4:1681481/149‑1 (MQ=255)
                                                           ccGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGAt                                                                                                          >  3:1550133/1‑131 (MQ=255)
                                                           ccGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGAt                                                                                                          <  4:1550133/131‑1 (MQ=255)
                                                             gTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAggg                                                                                                                                           <  1:644510/96‑1 (MQ=255)
                                                                  ttCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGc                                                                                                  >  2:422036/1‑132 (MQ=255)
                                                                  ttCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATcaca                                                                                 >  3:1294186/1‑149 (MQ=255)
                                                                  ttCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATcaca                                                                                 >  3:851086/1‑149 (MQ=255)
                                                                  ttCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACCCTGCAAGTCGATTTGCCCGCTTCCCGATACCATcaca                                                                                 >  4:684485/1‑149 (MQ=255)
                                                                   tCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAgg                                                                                                                                            <  3:744712/89‑1 (MQ=255)
                                                                        ttCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCt                                                                           >  4:35331/1‑149 (MQ=255)
                                                                        ttCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCt                                                                           >  2:792880/1‑149 (MQ=255)
                                                                           aCCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGcc                                                                        >  2:150816/1‑149 (MQ=255)
                                                                              aaCTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGt                                                                     >  2:1658167/1‑149 (MQ=255)
                                                                               aCTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATACTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATc                                                                                    >  3:356124/1‑133 (MQ=255)
                                                                                 tCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAAt                                                                  >  4:972917/1‑149 (MQ=255)
                                                                                 tCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAAt                                                                  >  1:400679/1‑149 (MQ=255)
                                                                                 tCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAAt                                                                  <  2:1030193/149‑1 (MQ=255)
                                                                                  cAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATg                                                                 <  4:1358539/149‑1 (MQ=255)
                                                                                   aGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGc                                                                <  4:724495/149‑1 (MQ=255)
                                                                                             gTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGa                                                      <  2:839009/149‑1 (MQ=255)
                                                                                             gTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGa                                                      <  1:1118332/149‑1 (MQ=255)
                                                                                             gTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGa                                                      <  1:1231446/149‑1 (MQ=255)
                                                                                             gTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGa                                                      <  1:1272576/149‑1 (MQ=255)
                                                                                             gTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTCTCGCTGa                                                      >  1:1630405/1‑149 (MQ=255)
                                                                                             gTCCTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGTGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGTTTTGCCCGCGTCCCGATACCATCACACCTGCTACCAGTAAGGCCTGTCGCTGa                                                      <  4:223388/149‑1 (MQ=255)
                                                                                              tCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGTGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGATGAc                                                     >  4:1077563/1‑149 (MQ=255)
                                                                                                ttCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGc                                                   >  4:1587160/1‑149 (MQ=255)
                                                                                                    gCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTa                                               >  2:603189/1‑149 (MQ=255)
                                                                                                    gCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTa                                               >  2:1091625/1‑149 (MQ=255)
                                                                                                     cAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGc                                                         >  3:1317934/1‑138 (MQ=255)
                                                                                                     cAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGc                                                         <  4:1317934/138‑1 (MQ=255)
                                                                                                      aGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATa                                                                                         <  2:1714835/105‑1 (MQ=255)
                                                                                                           cAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccgg                                        <  1:1699973/149‑1 (MQ=255)
                                                                                                               tCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccggtccg                                    >  4:299844/1‑149 (MQ=255)
                                                                                                                  tACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTcc                                                                                              <  4:1436045/88‑1 (MQ=255)
                                                                                                                     gCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGc                              >  1:1115295/1‑149 (MQ=255)
                                                                                                                      cAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCg                             >  3:170718/1‑149 (MQ=255)
                                                                                                                       aaTATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGAttt                                                                                                        >  4:572588/1‑73 (MQ=255)
                                                                                                                       aaTATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGAttt                                                                                                        <  3:572588/73‑1 (MQ=255)
                                                                                                                          atTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTAAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAAt                         >  4:1631190/1‑149 (MQ=255)
                                                                                                                             aaGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATtgt                      >  4:677897/1‑149 (MQ=255)
                                                                                                                               gCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGc                    >  1:1744063/1‑149 (MQ=255)
                                                                                                                                 gACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCg                  >  3:64956/1‑149 (MQ=255)
                                                                                                                                 gACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTACAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCg                  >  1:1053649/1‑149 (MQ=255)
                                                                                                                                   cGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTg                <  1:1459473/149‑1 (MQ=255)
                                                                                                                                    gTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTgg               <  1:1562863/149‑1 (MQ=255)
                                                                                                                                    gTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTCTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTgg               <  2:1630405/149‑1 (MQ=255)
                                                                                                                                     ttAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGc              >  3:1414159/1‑149 (MQ=255)
                                                                                                                                     ctAACCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGc              >  1:298620/2‑149 (MQ=255)
                                                                                                                                      tAACCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCg             >  3:890572/1‑149 (MQ=255)
                                                                                                                                       aaCCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGAGGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGa            >  3:174895/1‑149 (MQ=255)
                                                                                                                                       aaCCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGa            >  1:1359904/1‑149 (MQ=255)
                                                                                                                                        aCCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAAtt                        <  2:1400089/136‑1 (MQ=255)
                                                                                                                                        aCCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccggtc                                      >  1:47214/1‑122 (MQ=255)
                                                                                                                                        aCCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGAt           >  3:318449/1‑149 (MQ=255)
                                                                                                                                         cccTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGAt                                                                                          <  3:1629761/69‑1 (MQ=255)
                                                                                                                                         cccTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCt                                                  <  4:363784/109‑1 (MQ=255)
                                                                                                                                         cccTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGAtt          >  3:1088239/1‑149 (MQ=255)
                                                                                                                                         cccTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGAtt          >  1:771829/1‑149 (MQ=255)
                                                                                                                                          ccTCTCCGGGCGGAGAGGGGTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATTCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTa         >  3:460137/1‑149 (MQ=255)
                                                                                                                                          ccTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTa         >  3:373341/1‑149 (MQ=255)
                                                                                                                                          ccTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTa         >  2:1361133/1‑149 (MQ=255)
                                                                                                                                           ctctCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTaa        <  2:485597/149‑1 (MQ=255)
                                                                                                                                           ctctCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTaa        >  4:5533/1‑149 (MQ=255)
                                                                                                                                           ctctCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTaa        <  1:603189/149‑1 (MQ=255)
                                                                                                                                            tctcCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAac       <  3:972917/149‑1 (MQ=255)
                                                                                                                                              tcCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACATCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAacca     <  4:851086/149‑1 (MQ=255)
                                                                                                                                               ccGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTa                                <  2:451022/121‑1 (MQ=255)
                                                                                                                                               ccGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTa                                >  1:451022/1‑121 (MQ=255)
                                                                                                                                               ccGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAaccac    >  4:1697837/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ATTGGTGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACCC  >  NC_000913/475825‑476118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: