Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,153,208 T→C 7.8% intergenic (+28/‑92) fabF → / → pabC 3‑oxoacyl‑[acyl‑carrier‑protein] synthase II/4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,153,2080TC 7.8% 744.2 / 36.6 242intergenic (+28/‑92)fabF/pabC3‑oxoacyl‑[acyl‑carrier‑protein] synthase II/4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
Reads supporting (aligned to +/- strand):  ref base T (116/107);  new base C (10/9);  total (126/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

AGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCA  >  NC_000913/1153060‑1153353
                                                                                                                                                    |                                                                                                                                                 
aGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGt                                                                                                                                                   <  2:409196/149‑1 (MQ=255)
  gTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGtac                                                                                                                                                 >  1:1638092/1‑147 (MQ=255)
       gATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGcaa                                                                                                                                             >  3:1240051/1‑146 (MQ=255)
           tGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCggg                                                                                                                                        >  4:1627500/1‑149 (MQ=255)
             gATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGcc                                                                                                                                      >  1:1387849/1‑149 (MQ=255)
               tttCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGGGGCACTAATGGTTCTTTGATCTTTAAAAAGAGCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCtt                                                                                                                                    >  4:497489/1‑149 (MQ=255)
                  cGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCttttt                                                                                                                                 >  1:90900/1‑149 (MQ=255)
                  cGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCttttt                                                                                                                                 <  3:205167/149‑1 (MQ=255)
                   gTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCtttttt                                                                                                                                >  4:239359/1‑149 (MQ=255)
                   gTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGCCCGCAAGCGGACCttttat                                                                                                                                <  3:835159/149‑3 (MQ=255)
                       cGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTa                                                                                                                            >  1:728559/1‑149 (MQ=255)
                        gtaggAAGAGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAg                                                                                                                           >  4:1290292/5‑149 (MQ=255)
                        gCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAg                                                                                                                           >  2:1418714/1‑149 (MQ=255)
                            gAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTc                                                                                                                               <  2:11633/141‑1 (MQ=255)
                            gAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCttt                                                                                                                       <  4:112967/149‑1 (MQ=255)
                                            gCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTTAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTcc                                                                                                       <  4:1496123/149‑1 (MQ=255)
                                            gCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTcc                                                                                                       <  4:1054276/149‑1 (MQ=255)
                                                  tggaatACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTg                                                                                                 >  3:105511/1‑149 (MQ=255)
                                                     aatACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAAc                                                                                              <  3:379484/149‑1 (MQ=255)
                                                     aatACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGCCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAAc                                                                                              <  3:760678/149‑1 (MQ=255)
                                                        acacTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTGCCGTAGTGAACAtg                                                                                           <  2:675646/149‑1 (MQ=255)
                                                          acTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACAtgct                                                                                         >  3:443956/1‑149 (MQ=255)
                                                            tctGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACAtgctgc                                                                                       <  2:45140/149‑1 (MQ=255)
                                                            tctGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGCCCGCAAGCgga                                                                                                                                        >  3:1526380/1‑99 (MQ=255)
                                                                 gtAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGtt                                                                                                         <  2:1035586/126‑1 (MQ=255)
                                                                 gtAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGtt                                                                                                         >  1:1035586/1‑126 (MQ=255)
                                                                 gtAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCacac                                                                                  <  1:560165/149‑1 (MQ=255)
                                                                  tAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTc                                                                                                                                                  >  2:779332/1‑84 (MQ=255)
                                                                    aCTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTaa                                                                               >  2:231270/1‑149 (MQ=255)
                                                                        cttcggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAAt                                                                           >  3:1034580/1‑149 (MQ=255)
                                                                        cttcggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAAt                                                                           >  1:1544700/1‑149 (MQ=255)
                                                                         ttcggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAAtt                                                                          >  2:1685594/1‑149 (MQ=255)
                                                                            ggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATtctc                                                                       >  1:1284346/1‑149 (MQ=255)
                                                                             gcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTctct                                                                      >  4:592741/1‑149 (MQ=255)
                                                                              cttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTg                                                                     <  1:502574/149‑1 (MQ=255)
                                                                               ttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATtct                                                                        >  3:1597382/1‑145 (MQ=255)
                                                                                 cggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATa                                                                  <  1:10117/149‑1 (MQ=255)
                                                                                  ggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATaa                                                                 <  3:310636/149‑1 (MQ=255)
                                                                                  ggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATaa                                                                 <  2:1090050/149‑1 (MQ=255)
                                                                                  ggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCGCTGATaa                                                                 <  2:364339/149‑1 (MQ=255)
                                                                                        aCTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGcc                                                           >  3:286212/1‑149 (MQ=255)
                                                                                        aCTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGcc                                                           <  3:1406468/149‑1 (MQ=255)
                                                                                        aCTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGcc                                                           >  3:267929/1‑149 (MQ=255)
                                                                                        aCTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGcc                                                           <  2:1092817/149‑1 (MQ=255)
                                                                                         cTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCg                                                          >  1:1749120/1‑149 (MQ=255)
                                                                                             tGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCAGGCCGTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTAt                                                      <  3:1627500/149‑1 (MQ=255)
                                                                                                ttCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGtt                                                   >  1:816930/1‑149 (MQ=255)
                                                                                                ttCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGtt                                                   >  2:75235/1‑149 (MQ=255)
                                                                                                  cTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGAt                                                                   <  1:1456267/131‑1 (MQ=255)
                                                                                                  cTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGAt                                                                   >  2:1456267/1‑131 (MQ=255)
                                                                                                  cTTAGATCTTTTTAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCt                                                 >  3:713674/1‑149 (MQ=255)
                                                                                                   tttGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCtt                                                >  1:38661/1‑149 (MQ=255)
                                                                                                   tttGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCtt                                                <  4:669446/149‑1 (MQ=255)
                                                                                                   tttGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCtt                                                >  3:560571/1‑149 (MQ=255)
                                                                                                   ttagATCTTTTTAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCtt                                                >  2:1717599/4‑149 (MQ=255)
                                                                                                    ttGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCtta                                               <  1:170942/149‑1 (MQ=255)
                                                                                                     tGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCttaa                                              <  2:1515926/149‑1 (MQ=255)
                                                                                                        tCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTTATAAGGAGCCGGTATGTTCttaatta                                           >  1:109036/1‑149 (MQ=255)
                                                                                                        tCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCttaatta                                           >  3:1083468/1‑149 (MQ=255)
                                                                                                         cTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCttaattaa                                          >  3:90946/1‑149 (MQ=255)
                                                                                                          tttAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCtt                                                <  1:1266909/142‑1 (MQ=255)
                                                                                                          tttAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAAc                                         >  2:552518/1‑149 (MQ=255)
                                                                                                          tttAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAAc                                         <  1:1418714/149‑1 (MQ=255)
                                                                                                          tttAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACg                                        >  1:336812/1‑149 (MQ=255)
                                                                                                           ttAAAAAGATCTAAGTTGTCATTGTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACg                                        <  2:1368264/149‑1 (MQ=255)
                                                                                                            tAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACgg                                       <  1:1167575/149‑1 (MQ=255)
                                                                                                            tAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACgg                                       <  4:1083468/149‑1 (MQ=255)
                                                                                                             aaaaaGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGt                                      <  3:990742/149‑1 (MQ=255)
                                                                                                              aaaaGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTc                                     >  1:125287/1‑149 (MQ=255)
                                                                                                                  gATCTAAGTTGGCATTTTCCACCCTTCGAAAAGGTCCGCTTGCGGGCCTTTTTACTGAGCCTGTATTCCGACCTGTTCCGTAGTGAACATGCGGCCACACTAACAACTCTCCGATAAGGAGCCGGTATGTTCTTAATTAACGGGCATaa                                 <  4:1719811/149‑1 (MQ=255)
                                                                                                                   aTCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAg                                <  3:630869/149‑1 (MQ=255)
                                                                                                                     cTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCa                              <  1:1717599/149‑1 (MQ=255)
                                                                                                                      tAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAg                             <  1:1337497/149‑1 (MQ=255)
                                                                                                                        aGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTc                                     >  4:1195315/1‑139 (MQ=255)
                                                                                                                        aGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACGTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGTTAAGGAGTCGGTATGTTCTTAATTAACGGTCATAAGCAGGa                           >  4:661420/1‑149 (MQ=255)
                                                                                                                          ttGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAAt                         >  1:1345177/1‑149 (MQ=255)
                                                                                                                            gTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCg                       >  4:1547698/1‑149 (MQ=255)
                                                                                                                              cATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCt                     >  2:1244140/1‑149 (MQ=255)
                                                                                                                               aTTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTg                    >  4:887775/1‑149 (MQ=255)
                                                                                                                               aTTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTg                    <  2:1654485/149‑1 (MQ=255)
                                                                                                                                ttttCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTgg                   <  3:1194134/149‑1 (MQ=255)
                                                                                                                                ttttCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTgg                   >  1:969489/1‑149 (MQ=255)
                                                                                                                                ttttCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTgg                   <  1:262145/149‑1 (MQ=255)
                                                                                                                                ttttCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTgg                   >  4:310458/1‑149 (MQ=255)
                                                                                                                                   tCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAg                >  4:803035/1‑149 (MQ=255)
                                                                                                                                     cACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTa              >  2:102366/1‑149 (MQ=255)
                                                                                                                                      aCCCTTATAAAAGGTCCGCTTGCGGTACTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTACTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTaa             <  3:679773/149‑1 (MQ=255)
                                                                                                                                      aCCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTaa             <  2:1170170/149‑1 (MQ=255)
                                                                                                                                       cccTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAg            >  2:1269134/1‑149 (MQ=255)
                                                                                                                                        ccTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAAc                                                                                              <  3:1560530/66‑1 (MQ=255)
                                                                                                                                        ccTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAAc                                                                                              >  4:1560530/1‑66 (MQ=255)
                                                                                                                                        ccTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGc           >  2:1615624/1‑149 (MQ=255)
                                                                                                                                            ataAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATc       <  4:615908/149‑1 (MQ=255)
                                                                                                                                             taAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCg      <  1:769058/149‑1 (MQ=255)
                                                                                                                                              aaaaGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAg                                <  3:1143547/122‑1 (MQ=255)
                                                                                                                                              aaaaGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAg                                >  4:1143547/1‑122 (MQ=255)
                                                                                                                                              aaaaGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCgg     >  1:1098468/1‑149 (MQ=255)
                                                                                                                                               aaaGGTCCGCTTGCGGGCCTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGc   >  1:122774/1‑149 (MQ=255)
                                                                                                                                                 aGGCCCGCAAGCGGACCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCa  >  4:410595/1‑149 (MQ=255)
                                                                                                                                                   gTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACg                                        <  1:432257/109‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCA  >  NC_000913/1153060‑1153353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: