Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,153,219 G→A 7.1% intergenic (+39/‑81) fabF → / → pabC 3‑oxoacyl‑[acyl‑carrier‑protein] synthase II/4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,153,2190GA 7.1% 757.5 / 31.0 239intergenic (+39/‑81)fabF/pabC3‑oxoacyl‑[acyl‑carrier‑protein] synthase II/4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
Reads supporting (aligned to +/- strand):  ref base G (112/109);  new base A (9/8);  total (121/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 252 total aligned reads displayed.

TGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGA  >  NC_000913/1153071‑1153367
                                                                                                                                                    |                                                                                                                                                    
    tttCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCtt                                                                                                                                                  >  1:458451/1‑149 (MQ=255)
    tttCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGGGGCACTAATGGTTCTTTGATCTTTAAAAAGAGCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCtt                                                                                                                                                  >  4:497489/1‑149 (MQ=255)
     ttCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGCCCGCAAGCGGACCttt                                                                                                                                                 <  2:1161157/149‑1 (MQ=255)
      tCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCtttt                                                                                                                                                <  4:213218/149‑1 (MQ=255)
       cGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCttttt                                                                                                                                               >  1:90900/1‑149 (MQ=255)
       cGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCttttt                                                                                                                                               <  3:205167/149‑1 (MQ=255)
           ccGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCtt                                                                                                                                           >  3:840385/1‑149 (MQ=255)
            cGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTa                                                                                                                                          <  3:1576660/149‑1 (MQ=255)
                 gAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCttt                                                                                                                                     <  4:112967/149‑1 (MQ=255)
                     cgcgTCAGGGTAGCGGAATGGAATACACACTGTCTAACTCCTTCGGCTTCCGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTAtt                                                                                                                                 <  2:1432427/149‑1 (MQ=255)
                         tCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGa                                                                                                                             <  4:898225/149‑1 (MQ=255)
                         tCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGa                                                                                                                             <  4:1443817/149‑1 (MQ=255)
                                 gCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTcc                                                                                                                     <  4:1054276/149‑1 (MQ=255)
                                      atggaatACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGt                                                                                                                <  3:1684875/149‑1 (MQ=255)
                                          aatACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAAc                                                                                                            >  1:1515926/1‑149 (MQ=255)
                                                 tctGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACAtgctgc                                                                                                     <  2:45140/149‑1 (MQ=255)
                                                 tctGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACAtgctgc                                                                                                     <  1:129737/149‑1 (MQ=255)
                                                      gtAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGtt                                                                                                                       <  2:1035586/126‑1 (MQ=255)
                                                      gtAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCacac                                                                                                <  2:671622/149‑1 (MQ=255)
                                                             cttcggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGtt                                                                                                                       <  1:1216879/119‑1 (MQ=255)
                                                             cttcggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGtt                                                                                                                       >  2:1216879/1‑119 (MQ=255)
                                                                 ggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATtctc                                                                                     <  3:685902/149‑1 (MQ=255)
                                                                 ggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATtctc                                                                                     >  1:1284346/1‑149 (MQ=255)
                                                                 ggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATtctc                                                                                     <  1:1351808/149‑1 (MQ=255)
                                                                  gcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTctct                                                                                    >  4:592741/1‑149 (MQ=255)
                                                                   cttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTg                                                                                   <  1:502574/149‑1 (MQ=255)
                                                                    ttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATtct                                                                                      <  4:1597382/145‑1 (MQ=255)
                                                                    ttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATtct                                                                                      >  3:1597382/1‑145 (MQ=255)
                                                                    ttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGa                                                                                  <  1:1098025/149‑1 (MQ=255)
                                                                      cggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATa                                                                                <  1:10117/149‑1 (MQ=255)
                                                                        gTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTTATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAg                                                                              <  2:679508/149‑1 (MQ=255)
                                                                        gTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAg                                                                              >  3:615908/1‑149 (MQ=255)
                                                                             aCTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGcc                                                                         <  3:1406468/149‑1 (MQ=255)
                                                                              cTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCg                                                                        >  1:1749120/1‑149 (MQ=255)
                                                                               tAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACt                                                                                                                           <  4:756883/97‑1 (MQ=255)
                                                                                   ggTTCTTTGGTCTTGAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGAGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATg                                                                   >  2:1065509/1‑149 (MQ=255)
                                                                                    gTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGt                                                                  >  1:1626233/1‑149 (MQ=255)
                                                                                    caacttagATCTTTTTAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGt                                                                  >  2:1687178/8‑149 (MQ=255)
                                                                                     ttCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGtt                                                                 >  2:75235/1‑149 (MQ=255)
                                                                                     ttCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGtt                                                                 >  1:816930/1‑149 (MQ=255)
                                                                                       cTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGAt                                                                                 >  2:1456267/1‑131 (MQ=255)
                                                                                       cTTAGATCTTTTTAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCt                                                               >  3:713674/1‑149 (MQ=255)
                                                                                        tttGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCtt                                                              >  3:560571/1‑149 (MQ=255)
                                                                                        tttGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCtt                                                              <  4:1556592/149‑1 (MQ=255)
                                                                                        tttGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCtt                                                              >  1:38661/1‑149 (MQ=255)
                                                                                        tttGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCtt                                                              <  4:669446/149‑1 (MQ=255)
                                                                                        ttagATCTTTTTAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCtt                                                              >  2:1717599/4‑149 (MQ=255)
                                                                                         ttGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCtta                                                             <  1:170942/149‑1 (MQ=255)
                                                                                          tGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCttaa                                                            <  2:1515926/149‑1 (MQ=255)
                                                                                             tCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTTATAAGGAGCCGGTATGTTCttaatta                                                         >  1:109036/1‑149 (MQ=255)
                                                                                               tttAAAAAGATCTAAGTTGTCATTTTCCACCCTTATACAAGGTTCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTGTGATAAGGAGCCGGTATGTCCTTACTTAAc                                                       <  1:963565/149‑1 (MQ=255)
                                                                                               tttAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCtt                                                              >  2:1266909/1‑142 (MQ=255)
                                                                                                ttAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACg                                                      <  3:1174877/149‑1 (MQ=255)
                                                                                                ttAAAAAGATCTAAGTTGTCATTGTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACg                                                      <  2:1368264/149‑1 (MQ=255)
                                                                                                 tAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACCATTCTCTGATCAGGAGCCGGTATGTTCTTAATTAACgg                                                     <  2:109036/149‑1 (MQ=255)
                                                                                                 tAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACgg                                                     <  4:1083468/149‑1 (MQ=255)
                                                                                                 tAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACgg                                                     <  1:1167575/149‑1 (MQ=255)
                                                                                                  aaaaaGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGt                                                    <  3:990742/149‑1 (MQ=255)
                                                                                                   aaaaGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTc                                                   >  1:125287/1‑149 (MQ=255)
                                                                                                    aaaGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCa                                                  >  1:1343764/1‑149 (MQ=255)
                                                                                                         tCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGc                                             >  1:318927/1‑149 (MQ=255)
                                                                                                          cTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGCCTTGTTCCGTAGTTAACATGCTGCCACACTAACAAGTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACTGTCATAAGCa                                            <  3:1032965/149‑1 (MQ=255)
                                                                                                          cTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCa                                            <  1:1717599/149‑1 (MQ=255)
                                                                                                          cTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCa                                            <  1:681623/149‑1 (MQ=255)
                                                                                                           tAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAg                                           >  3:1036320/1‑149 (MQ=255)
                                                                                                           tAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAg                                           <  3:30261/149‑1 (MQ=255)
                                                                                                             aGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTc                                                   >  4:1195315/1‑139 (MQ=255)
                                                                                                             aGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTc                                                   <  3:1195315/139‑1 (MQ=255)
                                                                                                             aGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACGTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGTTAAGGAGTCGGTATGTTCTTAATTAACGGTCATAAGCAGGa                                         >  4:661420/1‑149 (MQ=255)
                                                                                                              attGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTCTCCTTTATTCCGACTTGTTCGGCAGTGAACATGCTCTCACAGTACCAATTTTCTTCTAAGGAGCCGGTCTGTTCTTAATTATCGTTGATAAGCAGGaa                                        >  4:1512866/2‑149 (MQ=255)
                                                                                                               ttGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTAt                                                                    >  3:1076408/1‑120 (MQ=255)
                                                                                                                  tCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGc                                    >  3:773612/1‑149 (MQ=255)
                                                                                                                    aTTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTg                                  >  3:1138902/1‑149 (MQ=255)
                                                                                                                    aTTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTg                                  <  2:1654485/149‑1 (MQ=255)
                                                                                                                     ttttCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTgg                                 >  2:1165080/1‑149 (MQ=255)
                                                                                                                     ttttCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTgg                                 >  4:310458/1‑149 (MQ=255)
                                                                                                                        tCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAg                              >  4:803035/1‑149 (MQ=255)
                                                                                                                          cACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTa                            >  2:102366/1‑149 (MQ=255)
                                                                                                                           aCCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTgg                                 <  3:416441/143‑1 (MQ=255)
                                                                                                                           aCCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTaa                           >  3:373943/1‑149 (MQ=255)
                                                                                                                           aCCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTaa                           >  3:270139/1‑149 (MQ=255)
                                                                                                                             ccTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGc                         >  2:1615624/1‑149 (MQ=255)
                                                                                                                             ccTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGc                         >  1:140469/1‑149 (MQ=255)
                                                                                                                                gaaaaaaGGCCCGCAAGCGGACCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGAt                      >  1:1441931/4‑149 (MQ=255)
                                                                                                                                 ataAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATc                     >  2:386456/1‑149 (MQ=255)
                                                                                                                                 ataAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATc                     <  3:929055/149‑1 (MQ=255)
                                                                                                                                 ataAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATc                     <  4:615908/149‑1 (MQ=255)
                                                                                                                                  tagaaGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATGCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCg                    <  4:1170218/146‑1 (MQ=255)
                                                                                                                                  taAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCg                    >  2:356300/1‑149 (MQ=255)
                                                                                                                                  taAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCg                    <  1:769058/149‑1 (MQ=255)
                                                                                                                                   aaaaGGCCCGCAAGCGGACCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCgg                   >  1:1261277/1‑149 (MQ=255)
                                                                                                                                    aaaGGCCCGCAAGCGGACCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGt                                                                  <  4:1685799/101‑1 (MQ=255)
                                                                                                                                      aGGCCCGCAAGCGGACCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCa                >  4:410595/1‑149 (MQ=255)
                                                                                                                                           cGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATc                                      <  2:878298/122‑1 (MQ=255)
                                                                                                                                           cGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCa           <  2:1343764/149‑1 (MQ=255)
                                                                                                                                              ttGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGtt        <  1:103553/149‑1 (MQ=255)
                                                                                                                                                gCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTg      <  1:231270/149‑1 (MQ=255)
                                                                                                                                                  gggCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGt    <  2:1665626/149‑1 (MQ=255)
                                                                                                                                                  gggCCTCTTTTCTTAGCTTTTATTCCGACTTGTTCCCTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATAGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGt    <  3:1525940/149‑1 (MQ=255)
                                                                                                                                                   ggCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTg   >  4:382141/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGA  >  NC_000913/1153071‑1153367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: