Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,2680TC25.6% 56.3 / 16.9 46intergenic (+474/+168)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base T (15/17);  new base C (8/3);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAA  >  NC_000913/4295999‑4296540
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                    
tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGTGTTTATGCCGCATCCGACATCAACGCCTGATGTGACGTTTAACGCGCCTTATCAGGACTACGCAAGACAGAGCAATAGCCTGCTTTAGCGTGACTTTGTAGGTCGGATAAGGCGTTTACGACGCa                                                                                                                                                                                                                                                                                >  2:511737/1‑276 (MQ=11)
     tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTCTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGGGATTTTGTAGGTCGTATAAGGCGTTTACGCCGCCTCCg                                                                                                                                                                                                                                                                            >  1:452903/1‑275 (MQ=255)
                 aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACTGCGCAATAGCTTGATTTAGCGTGATTTTGTAGTCTGTATAAGACGTTTACGCCCCAGCCGACCACAATGcc                                                                                                                                                                                                                                                                 >  1:356139/1‑274 (MQ=11)
                                    gCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGTAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACACCAATCCCTGATGCGACCCTTGCcgcg                                                                                                                                                                                                                                              >  3:275099/1‑274 (MQ=255)
                                     cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGCATAAGGCGTTTCTGCCGCATCCGACATCAACGCCTGATGCGACGATTAACGCGTCTTATCAGGCCTACGCCAGACAGCCACATAGCCTGATTTAGCGTGATTCTGTAGGTCGGACAAGGCGTTTACGCCGCATCCGACAACACTGCCTGATGCTACGCttgccgcccc                                                                                                                                                                                                                                            >  4:429222/1‑267 (MQ=11)
                                        ttACGCCGCATCCGACAGCCACGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTCAATCGACGATTAACGCGTCTTATCCGGCCTCCGCCAGCCAGCTACATAGCCTGATTTAGCGTGATTTTCTAGGTCTGATAACTCGTTTACGCCGTCGCCGATCTCCATGCCTGTTGCGCCGCTTACCGCCTCCTa                                                                                                                                                                                                                                         >  3:164040/1‑275 (MQ=11)
                                        ttACCCCGCATACGACATCAACGCTGTATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                                                                                                                                        <  2:469607/276‑1 (MQ=255)
                                         tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                                                                                                                                         >  1:36628/1‑274 (MQ=17)
                                         tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                                                                                                                                        >  2:59927/1‑275 (MQ=17)
                                              cgcATCCTACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAGCAGGCCTACGCCAGACAGGGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                                                                   <  2:375146/275‑1 (MQ=9)
                                              cgcATCCGACCTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                                                                                                                                  <  2:517935/276‑1 (MQ=9)
                                                                                                                                                          tacgCCGCATACGACGTCAACGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACGCGAGACAGCGCAATAGCCTGATGTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAGGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                      <  3:393893/271‑1 (MQ=11)
                                                                                                                                                                                           gcttgccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                   <  2:249279/270‑1 (MQ=255)
                                                                                                                                                                                           gctgggcgggTCTTATCAGGCCTATGCCGGAAAGCGCAAGAGCCTGATTTAGCGTGAATTTGTAGGTCGGATAAGGCGTTTATGCCGAATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                   <  2:519304/267‑1 (MQ=11)
                                                                                                                                                                                           gcaggcggcgTTTTAGCAGGCGTCCGCAAGGCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCAGCAGCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCGACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                   <  4:258337/269‑1 (MQ=11)
                                                                                                                                                                                                       gtATCAGGCCTAGTGCAGACAGCGCAATATCCTGCTTTTGCGTGATTTTGTAGGTAGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                        <  2:374900/274‑1 (MQ=14)
                                                                                                                                                                                                        tATCAGGCCTACGCCAGACAGCGCAATTGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                        <  1:417474/274‑1 (MQ=255)
                                                                                                                                                                                                        tATCAGGCATACGCCAGACAGCGCGATAGCCTGATTTAGCGTGATTTTGTAGGTCGAATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGGCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                        <  1:426634/274‑1 (MQ=255)
                                                                                                                                                                                                          tCAGACCTACGCCAGAAGACGTAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAAGCCGCATCCGACATCCACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGGCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACGcc                                                                        <  1:255961/272‑1 (MQ=14)
                                                                                                                                                                                                              gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCTTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGTGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCGGCCATCAATGCCTGCTCCGACGCTGTCCGCGTCTTATCCCGCCTATCTTAACCGTTAGATAATTTTCCACACTCTTCACAcctgctacc                                                                 >  4:526582/1‑268 (MQ=11)
                                                                                                                                                                                                                 tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                              <  2:462330/275‑1 (MQ=255)
                                                                                                                                                                                                                 gacgcgaaggagagcAATGGCCGGAGTGAGGGTGATTTTTTGAGTCTGCTAAGGCGTTTATGCCGGACCCGACATCAACGCCTGATGCGACGCTTAGCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                              <  2:259804/262‑1 (MQ=14)
                                                                                                                                                                                                                  acaacAGAAAGCAGAATAGCCTGATTTAGCGTGATATTGTAGGTAGCATAAGGCGTGAAGGCCGCATCAGAAATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                              <  3:479156/270‑1 (MQ=14)
                                                                                                                                                                                                                            gcgcAATAGCCTGATTTAGCGTGTTTTTGTAGGTCGGATAAGGCGTTTATGCCGCCTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCAt                                                     <  2:147126/273‑1 (MQ=255)
                                                                                                                                                                                                                             cgcAATCGCCTGATTTAGCGTGATTTTGTAGGTCCGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATTGGACGCGTACCCAGTCTTCCCAGGCCTATGCTAGCCAGCGCAAAAGCTTGCTTTACCGTGATGTTGTAGATCGCATAGGGCGTTTA‑‑CGCGTCATCCAAAATAGATGACTACTGTGTCGCTTGCCGCGTCTGATCAGCCCTATCTTACCTGTAGGTCACTTTTTGAAACTCTTCACACCCGTTTCCAAACCCTTCCATAcc                                                  >  3:329333/1‑274 (MQ=9)
                                                                                                                                                                                                                             cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACCTCAATGCCTGATGCGACCCTTGCCGCGTCTTATAAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTAACACCCGTTCACAACCCCTTccctcca                                                  >  3:306044/1‑270 (MQ=14)
                                                                                                                                                                                                                             cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCTGTATCCAACCCGTCCCTaaa                                                  >  3:543635/1‑273 (MQ=255)
                                                                                                                                                                                                                             cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACAGCAATGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTATCTTCACCGTTGGCTAATTTTTCAAACTCTTCCCACCAGGTACCAAACCCTTCCATACa                                                  >  2:593275/1‑275 (MQ=14)
                                                                                                                                                                                                                             cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTCATTTTGCAGGTCGGAGAAGGCGTTTACGC‑CG‑CATCCGACACAAACGCCTGCGACGCATCATGCCGCGTCTTAACAGGCCTATCTTAACCGTTGGTTAATTTTTCCAACTCCTCCACCCCGGTACCAACCCCTTCCAtcc                                                   >  3:629488/1‑272 (MQ=14)
                                                                                                                                                                                                                               cAATCGCCTGATTAAGCGGGATTTTGTAGGGCGGATAAGGCGAGAATGCCGCAACCGACATCAACTCCGGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                               <  2:181244/276‑1 (MQ=17)
                                                                                                                                                                                                                                aaTAGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                               <  1:398098/275‑1 (MQ=255)
                                                                                                                                                                                                                                     ccTGATGTAGCGTGATTTTGTAGTTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGCCGCTTAACGCGTCTTATCAGGCCTAAGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                          <  1:187107/275‑1 (MQ=255)
                                                                                                                                                                                                                                       tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTc                                                                                      >  4:595126/1‑229 (MQ=255)
                                                                                                                                                                                                                                       tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAACAGGCGTTTACGC‑CG‑CATCCGCCATCAATGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTCATTTTTCAACCCCTTCACTCCCGGTATCAAACCCTTCCATACACCCCAGGtc                                         >  3:203316/1‑273 (MQ=14)
                                                                                                                                                                                                                                        gATTTATGGTGATTTTGTAGGTCGGCTAATGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                          <  1:429968/272‑1 (MQ=255)
                                                                                                                                                                                                                                          tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                   <  2:595126/229‑1 (MQ=255)
                                                                                                                                                                                                                                            tAGCGTGAGTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGAAATCAACGCCGGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                                     <  1:389120/273‑1 (MQ=255)
                                                                                                                                                                                                                                                tggATTTGGTAGGCCGGATAAGGCGTTTATGCCGCAACAGAAATCAGCGCCGGTTGCGCCGTTTACCGGGTCTTATCAGGCCCACGCCAGACAGCGCAATGGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCa                              <  4:169887/274‑1 (MQ=18)
                                                                                                                                                                                                                                                   aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg                             >  1:109807/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                 aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCCCCTTTTGCTTGt               >  3:76408/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                          ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATTGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGAGCTTACGC‑CG‑CATCCGACATCAATTCCTGATGCGACGCTTGCCGCGTCCTATCAGGCCTATCTTAACAGTTGGTTAATTTTTCACATTCTTCTACCCTGGTACAAAACACTTCCATAATGCATAGGTTCCACCGTTTCATGCCCTTTTCCTTGTTCTtctcca      >  3:272465/1‑272 (MQ=14)
                                                                                                                                                                                                                                                                           tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTATCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGTCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACACGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGCGCATCGCCTTTTGCTTGTTCTTCTCGAt     >  1:587540/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                           tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACTCGGTATCAACCCCTTCCATCCAGCTCAGGTTCAACCAGAGCACCGCCTTTTCCTTCTTCTTCTCGAt     >  3:327053/1‑274 (MQ=18)
                                                                                                                                                                                                                                                                           tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGAt     >  3:243742/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                           tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAATCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGTTTCAACCAGTCCAGCGCCTTTTGCTTGTTCTTCTCGATa    >  3:667872/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                           tATGCCGAATCCGAAATCAACGCCTGATGCGACGCTTAACGCGGCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACCCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATa    <  2:250433/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                             tGCCACGTCCAACATCAACGCCTGATGCGACGGTTACCGCTTCTTTTCCTGCCTCCGCCAGCACGCGCAATAGCCTTCTTTTGCGTGATTTTTTCTGTCTGCTCAGGCGTTTACGC‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATaaa  <  2:487689/275‑1 (MQ=21)
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                    
TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAA  >  NC_000913/4295999‑4296540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑