BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,005	T→G	D73A (GAT→GCT)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,005	0	T	G	98.6%	484.2 / NA	142	D73A (GAT→GCT)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (64/76); total (65/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

AGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCT  >  NC_000913/4116738‑4117266

 caaaTTCACCACGACGTGCACGCTCGCGAGGGCCTTGCACATGGTCGGAGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCATACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTg                                                                                                                                                                                                                                                               <  3:329254/273‑1 (MQ=255)

  cAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACtta                                                                                                                                                                                                                                                              >  4:236838/1‑273 (MQ=255)

  cAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGa                                                                                                                                                                                                                                                              >  1:405808/1‑275 (MQ=255)

          cacGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCt                                                                                                                                                                                                                                                      <  3:349633/275‑1 (MQ=255)

            cgacgTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCtt                                                                                                                                                                                                                                                     >  1:639702/1‑274 (MQ=255)

               cgTGCACGCTCGCGAGAGCCTTCCACATGTTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCATTCACCAGACCGGTATTGCTGCGTACATAATTTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACTATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGCCAATAGTGGTTTCACGATTGTTCGTTATAACGATAGCTGCAATTCGAGCGGTACGGATTTCGTCTCTCGc                                                                                                                                                                                                                                                 >  3:259938/1‑275 (MQ=255)

               cgTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGTTAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCg                                                                                                                                                                                                                                                  >  1:605446/1‑274 (MQ=255)

                gtcaaCGCCCGCGTGAGCCTTCCCAAGGGTCGATGATCAACTTCACCTTGGTGCCAGAAAATTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTCAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCa                                                                                                                                                                                                                                               <  4:8007/272‑1 (MQ=255)

                 tGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGGGCGGAACTGatactgtttcttttac                                                                                                                                                                                                                                               >  1:623843/1‑262 (MQ=255)

                 tGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAg                                                                                                                                                                                                                                              >  2:259987/1‑276 (MQ=255)

                 tGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGGGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCa                                                                                                                                                                                                                                               <  3:33790/275‑1 (MQ=255)

                    aCGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCAc                                                                                                                                                                                                                                           >  2:531572/1‑276 (MQ=255)

                          gcgGGAGCCTGCCACATGGTCGAGGATCCACTTCACTGTGGTGCCAGAAAATTACGGGTCAATCACCAGACCGGTAATGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTa                                                                                                                                                                                                                                      <  3:92732/275‑1 (MQ=255)

                           cgAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACCATGGTTCGTAATACCGATAGCTGCCATTTGATGGGAACTGATAGTGGGTTTTGGCAGCAATTCTaac                                                                                                                                                                                                                                    >  4:352619/1‑274 (MQ=255)

                                 ccTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGt                                                                                                                                                                                                                               <  2:38126/275‑1 (MQ=255)

                                  cTTCCACAGGGTCGAGGATCCACTTCACTGTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTg                                                                                                                                                                                                                              <  2:225462/275‑1 (MQ=255)

                                  cTTCCACAGGGTCGAGGAACCACTTCACGTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGt                                                                                                                                                                                                                               <  3:313358/274‑1 (MQ=255)

                                    tCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTACCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGTTAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCATCCCTTCTACCCGCGTgg                                                                                                                                                                                                                             >  4:225462/1‑274 (MQ=255)

                                                       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTTGGTGGCCCCGAtt                                                                                                                                                                                                         >  1:241953/1‑275 (MQ=255)

                                                       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGAtt                                                                                                                                                                                                         >  1:348219/1‑275 (MQ=255)

                                                       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACAAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTATGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTAACGCTGGTTAGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTAGCCAGCCATTCTACCACCTTGGAGCTTTTGGTTGCCAGGAtt                                                                                                                                                                                                         >  3:530697/1‑275 (MQ=255)

                                                           ttctgtGGGGCCAAAAAAGTCAGGGTAAAAC‑CTAGACCTGTATTGCTGCGGAGATAATCTTCTAAACCGTCAAGTTTTAAATGCTCGCAGCTTTCTGCGGGACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTGCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                                                                                                                                                                   <  4:493086/271‑1 (MQ=255)

                                                           cacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCCGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCGCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCACTTTGAGCGGAACTGCTATCGGTTTTCGCTAGCACTTCTACCAGCGTGGAGTTTTGCTTGGCGCACATTccct                                                                                                                                                                                                     >  3:15702/1‑274 (MQ=255)

                                                             cttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                                                                                                                                                                   <  2:236838/275‑1 (MQ=255)

                                                                 ggggCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCT‑‑GGATATAATCTTCTAAAACGTCACGTTTTAAATTCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGGCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                              <  1:10186/271‑1 (MQ=255)

                                                                 ggTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg                                                                                                                                                                                                <  1:51251/274‑1 (MQ=255)

                                                                  gTGCCAGAAAAGTAGGGGTCAATAACCAGACCGGTATTGCTACGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                              <  3:304605/275‑1 (MQ=255)

                                                                                 gggTCAATCACCAGACCGGTATTGGTGCGGATATAATTTTCTAAACCGTGACGTTTTAAATGCTCGCGGATTGTTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAcc                                                                                                                                                                               <  3:66713/275‑1 (MQ=255)

                                                                                 gggTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGGCACTTTTTAAATGCGCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGCTAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTGGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAcc                                                                                                                                                                               <  2:580678/275‑1 (MQ=255)

                                                                                   gTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTGGGGTGGCCCAGATTTCCATTGGGTCGGTTTTTACCCAAccgg                                                                                                                                                                             >  1:25316/1‑273 (MQ=255)

                                                                                       aTCAGCAGACCGGGATTGCTGCGGAAATAATCTACTAAACCGTCACGTTTTGAATGCTCGCAGATTTCTGTGGTACGACGGCACGGCCAGACAATGGAGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAAGAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtt                                                                                                                                                                          <  2:580776/274‑1 (MQ=255)

                                                                                       aTCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtt                                                                                                                                                                          <  2:123158/274‑1 (MQ=255)

                                                                                            cAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGCCAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGTCAATAGTGGTTTCACGCTGGTTCGTAATACTGATAGCTGCTATTTGTGCGGAACTGATATCGGATTTct                                                                                                                                                                                                                                                  >  4:21465/1‑196 (MQ=255)

                                                                                               aCCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGCTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAg                                                                                                                                                                  >  3:229509/1‑274 (MQ=255)

                                                                                                  ggTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGGCCAGATTTCCAGTGGGTCGTGTTCTACCCAACCTGGTTTTGTGTAGAtt                                                                                                                                                               >  2:69036/1‑274 (MQ=255)

                                                                                                    tATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTg                                                                                                                                                             >  1:447739/1‑274 (MQ=255)

                                                                                                                    acattcgAAAGCGACAGGTTCTAAATGCGCGGCGATGTCTGCTGTGCGCTGTGACGGCGCAACAAGGGCGTCATAGATAGGCTTGTCGGTTTGTTTTTCCCAGTGAATAGTGGTTTAAGGATGGTTCGTGATAACGATAGCTGCAATTTGAGCGGAACTGATATTGGCTTTCGCGAGAACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                           <  4:504850/273‑1 (MQ=255)

                                                                                                                    aTCTTCTAAACCCTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                           <  2:352619/276‑1 (MQ=255)

                                                                                                                      cttctAAACCGTAACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                         <  4:61549/276‑1 (MQ=255)

                                                                                                                       ttctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                         <  4:259987/275‑1 (MQ=255)

                                                                                                                       ttatAAAACGTCACGTTTTAAATGCGCGCAGATTTCTGCGGTACGACGGAACTGCCAGACAATGTCTTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                         <  1:15702/272‑1 (MQ=255)

                                                                                                                        tctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTTCgccccg                                                                                                                                       >  4:298556/1‑271 (MQ=255)

                                                                                                                        tctAAACCGTCACGTTTTAAATGATCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                         <  1:479042/274‑1 (MQ=255)

                                                                                                                         ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTTGCCCTGCgccccg                                                                                                                                       >  1:349559/1‑270 (MQ=255)

                                                                                                                         ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGATTTCGCCAGCACTTCTACCAGCGTGGGGGTTTGGGTGGCCCAGATTTCCATTGGGTCGTGGTTCACCCACACGTGTTTTGGGTAGATTGTCCCAAATTCGGGCTgtcccacg                                                                                                                                       >  3:158196/1‑268 (MQ=255)

                                                                                                                            aaCCTTTACGTTTTAAATGGTCGCAGATTTTTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                                                                                                   <  2:298556/276‑1 (MQ=255)

                                                                                                                             aCCGTCACTGTTTAAATGCTCGCAGATTTCGGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                    <  1:340402/274‑1 (MQ=255)

                                                                                                                             aCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                    <  1:105313/274‑1 (MQ=255)

                                                                                                                              ccGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                                                                                                   <  1:226774/274‑1 (MQ=255)

                                                                                                                                 tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTg                                                                                                                                                                                                  >  3:572612/1‑208 (MQ=255)

                                                                                                                                 tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTTTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACTCTATTGa                                                                                                                                >  3:121932/1‑274 (MQ=255)

                                                                                                                                 tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACTGGGTTTTGGGTGGCTTTGCTCAAATTCGCGCTGCGACACGCtttggat                                                                                                                               >  2:159223/1‑269 (MQ=255)

                                                                                                                                 tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCACAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTACAATTCGCGCTGAGACACGCTACTGata                                                                                                                              >  4:568636/1‑276 (MQ=255)

                                                                                                                                   ccGTTTTAACTTCTCGCATATTTTGTCGGGACGACGGCCCTGCCAGATCATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGAAAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGata                                                                                                                              <  4:536850/273‑1 (MQ=255)

                                                                                                                                   aCGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGat                                                                                                                               >  3:489719/1‑273 (MQ=255)

                                                                                                                                    cGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGa                                                                                                                                <  2:568636/271‑1 (MQ=255)

                                                                                                                                         tAAATGCTCGCAGATTTCTGCGGTACGACGTAACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATc                                                                                                                      <  2:389527/276‑1 (MQ=255)

                                                                                                                                             ttcTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCTTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                  <  2:442621/274‑1 (MQ=255)

                                                                                                                                             tGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                  <  2:435103/276‑1 (MQ=255)

                                                                                                                                             tGCTCGAAGATTTCTCGGGTACGCCGGCACTTCCAGCCAATGGCGTTAGAGGTAGGCTTGCCCGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                  <  4:582964/276‑1 (MQ=255)

                                                                                                                                             tGCTCCGAGATTTCTTGGGTACGGCGGCACTGCCAGACAATGGCGGTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTGTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                  <  4:32786/276‑1 (MQ=255)

                                                                                                                                              gCTCGCAGATTTTTGCGGTACGACGGCACTGCCCGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATAGCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCAAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                  <  1:638320/275‑1 (MQ=255)

                                                                                                                                              gCTCGCAGATTTCGGCGTGAAGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTTCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                  <  1:481367/275‑1 (MQ=255)

                                                                                                                                                 cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGTTTTGCTCAAATTCGCGCTGCGCTACGCTACTGAAATTTGCGTCGTGa                                                                                                                  >  1:271464/1‑272 (MQ=255)

                                                                                                                                                 cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGCTTTGATCAAATTTGCGCTCCGACTCGCTAATCATTTTGGCTTAGTGATcc                                                                                                               >  1:144748/1‑275 (MQ=255)

                                                                                                                                                 cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCCAATTCGCGCTGCGACACGCTAATGACATTGGCATCGTGATCCa                                                                                                              >  2:405652/1‑276 (MQ=255)

                                                                                                                                                            gCGGTTCGAGTGCACTCCCAGACAATGGCTTTTTGGGTTGGCTTGCATGTTTCTTTTTCCCAGGTAATAGTGCTTTCACGCTGTTTCGTATTACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCGTAGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcg                                                                                                   <  4:87384/276‑1 (MQ=255)

                                                                                                                                                                   tacgGCACTGCCAGAAAATGGCGTTATGGTTAGGTTTTCCGGTTTCTTTTTCCCAGACAATAGTGTTTTCACGCTGGTTCGTAATACCGATAGCGGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAg                                                                                             <  4:563007/274‑1 (MQ=255)

                                                                                                                                                                         aCTGCCAGGCAGTGGCGTTATAGATAGGCTTTCCGGTTTCTTTTTCCCAGACAATAGTGGTTGCACGCTGGTTCTTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                                      <  2:12937/276‑1 (MQ=255)

                                                                                                                                                                                  ccattgtgTTATATGTAGGCGTGACGGTTCCTGTTTCCCAGACCATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAAGTTGAGCCGAACTGATATCGGCTTTCGCCAGCACTGCTACCAGCGTGGCGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTgg                                                                              <  4:130511/268‑1 (MQ=255)

                                                                                                                                                                                  cAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACTACCGCGCGGGTGCTGGGGGTGCCCTgg                                                                              >  2:563007/1‑275 (MQ=255)

                                                                                                                                                                                     tGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTTGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGa                                                                          >  2:370681/1‑276 (MQ=255)

                                                                                                                                                                                     tGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGa                                                                          >  2:437419/1‑276 (MQ=255)

                                                                                                                                                                                           tatggttagGCTTTCCGGTTTCTTTTTCCCCGACAATAGTGGTTTCACGCTTGTTCTTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTATGCAGCACTTCTACCAGCGTGGAGCTTTGTGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCaa                                                                    <  4:251599/270‑1 (MQ=255)

                                                                                                                                                                                           tataGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCaa                                                                    >  2:435494/1‑276 (MQ=255)

                                                                                                                                                                                            ataggtagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCaa                                                                    <  3:605445/275‑1 (MQ=255)

                                                                                                                                                                                                      ttGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAttt                                                          <  4:437419/275‑1 (MQ=255)

                                                                                                                                                                                                       tGCCGGCTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCCCTGCGACCCGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCTAGCGTAACGATATAtttt                                                         >  1:409875/1‑275 (MQ=255)

                                                                                                                                                                                                        gCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAttttt                                                        >  1:231292/1‑275 (MQ=255)

                                                                                                                                                                                                           ggTTTCTTTTTCCCAAACAATAGTGGTTTCACGCTGGTGCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGata                                                               <  3:409875/265‑1 (MQ=255)

                                                                                                                                                                                                              ttCTTTTTCCCAGACAATAGTGGTTTCACTCTGGATCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGGGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGt                                                 >  2:451553/1‑276 (MQ=255)

                                                                                                                                                                                                               tCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAg                                                  <  1:229509/274‑1 (MQ=255)

                                                                                                                                                                                                                cTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTc                                                <  1:110848/275‑1 (MQ=255)

                                                                                                                                                                                                                    ttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATg                                             <  3:405808/274‑1 (MQ=255)

                                                                                                                                                                                                                             aaTAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTTATATCTGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCa                                                                                                                        <  3:289554/190‑1 (MQ=255)

                                                                                                                                                                                                                              tgaGTGGTGTCACGCGGGTTCGTAATACCTATAGCTGCAATTTGAGCGGAACTGATACCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTccc                                 <  2:173112/274‑1 (MQ=255)

                                                                                                                                                                                                                               tAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGatat                                                                                                                                                                                                                                                           <  3:142300/57‑1 (MQ=255)

                                                                                                                                                                                                                                ggtggttttcggttGGGTCGAAATACGAATAGCTGCAGGTTGGGGGGAACGGGTTGCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCATATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCGCAAATTCGCGCTGCTACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCg                                <  3:690924/262‑1 (MQ=255)

                                                                                                                                                                                                                                gggtgTTTCGCGCTGTGTCGTAATACCGATGGCTGCAATTTGAGCGGAACTGATTTCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                               <  4:154156/272‑1 (MQ=255)

                                                                                                                                                                                                                                 gttgTTTCAGCCTGGTTTGTAATACCGATTGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTTCCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                               <  3:25316/272‑1 (MQ=255)

                                                                                                                                                                                                                                 gTGATTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                               <  1:530697/275‑1 (MQ=255)

                                                                                                                                                                                                                                   tgTTTCACGCTGGTTCTGAATACCGATAGCTGCAATTTGGGCGGAACTGATGTCGGCTGTCTCCAGCACTTCTACCAGCGTGGAGCTTTGGTTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATGTGCTCAAATTCGCGCTTCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                             <  3:271464/274‑1 (MQ=255)

                                                                                                                                                                                                                                   ggTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCATGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                             <  1:259938/275‑1 (MQ=255)

                                                                                                                                                                                                                                     tttCTCGCTGTTTCGTAATACCGATAGCTACAATTTGCGCGGAACTGATATCTGCTTTCGCCAGCACTTCTACCCGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCa                          <  4:435494/276‑1 (MQ=255)

                                                                                                                                                                                                                                     tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCTACACGCTAATGATATTGGCATCGTGATCCATTACGCCCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCATTCATGTTTAATTGTCCCGTAGt                            >  1:609027/1‑274 (MQ=255)

                                                                                                                                                                                                                                         aCGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTACCCTGGTCGAGCGCAACGTTATATTTTTTTGCTTTCATGTCTAATTTTTCCggtgtctcgtt                      >  4:434239/1‑266 (MQ=255)

                                                                                                                                                                                                                                                       tactgactgCTGCAATTTGAGGGGAACTTTTTTCGGCTTTCGCCGTGACTTCTACCAGCGTGGGGCTTTGGGTGGCCCTGATTTCCATTGGGTCGTGTTCTACCCAGCCTGGTTTTGGGTAGATTTGCTCAAATTAGCGCTGCGACATGCTAATGATTTTGGCATCGTGATCCAGTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCttt         <  4:370681/267‑1 (MQ=255)

                                                                                                                                                                                                                                                          cGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTTAGTCATGTTTAATTGTCCCGTAGTCATATTAATGCGAAGCTTTTTGt     >  2:24290/1‑276 (MQ=255)

                                                                                                                                                                                                                                                           gATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGt     >  3:174192/1‑275 (MQ=255)

                                                                                                                                                                                                                                                            aTAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGCGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTTTCCCGTAGTCCTATTACAGCGAAGCTTTTTGTTc   >  2:5215/1‑276 (MQ=255)

AGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCT  >  NC_000913/4116738‑4117266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`