Predicted mutation
evidence seq id position mutation freq annotation gene description
JC NC_000913 2,536,378 Δ25 bp 55.1% [pdxK] [pdxK]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2536377127 (0.470)153 (0.580) 107/252 NT 55.1% intergenic (+34/+9) crr/pdxK glucose‑specific enzyme IIA component of PTS/pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase
?NC_000913 2536403 = 125 (0.470)coding (835/852 nt) pdxK pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase

Only 100 of 297 total aligned reads displayed.

TCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCT  >  NC_000913/2536255‑2536551
                                                                                                                                                    |                                                                                                                                                    
tctcCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAgcgg                                                                                                                                                      >  2:74896/1‑149 (MQ=255)
  tcCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAgcggcg                                                                                                                                                    >  1:2049488/1‑149 (MQ=255)
   tctaccctgactccggttgttatctccaacatggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcggcgg                                                                                                                                                   >  2:618998‑M2/146‑149 (MQ=255)
       ccctgactccggttgttatctccaacatggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCaaa                                                                                                                                               >  1:1077762‑M2/142‑149 (MQ=255)
        cctgactccggttgttatctccaacatggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCaaaa                                                                                                                                              >  4:420535‑M2/141‑149 (MQ=255)
          tgactccggttgttatctccaacatggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATc                                                                                                                                            <  3:2369548‑M2/11‑1 (MQ=255)
          ggACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATc                                                                                                                                            <  4:509529/149‑1 (MQ=255)
           gactccggttgttatctccaacatggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCa                                                                                                                                           >  3:103155‑M2/138‑149 (MQ=255)
            actccggttgttatctccaacatggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCaa                                                                                                                                          <  3:2261625‑M2/13‑1 (MQ=255)
            aCGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCaa                                                                                                                                          <  4:724947/149‑1 (MQ=255)
            aCGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCaa                                                                                                                                          <  2:373308/149‑1 (MQ=255)
              gAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAAtt                                                                                                                                        >  4:89108/1‑149 (MQ=255)
                cggttgttatctccaacatggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCaaaa                                                                                                                                              >  3:2396068‑M2/133‑141 (MQ=255)
                aaTCAAAGAACTGATCAAACTGTCGGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAATATCAATTCa                                                                                                                                      >  3:2095600/1‑149 (MQ=255)
                 aTCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCAt                                                                                                                                     >  1:1600642/1‑149 (MQ=255)
                  gttgttatctccaacatggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATc                                                                                                                                    <  2:2089333‑M2/19‑1 (MQ=255)
                   ttgttatctccaacatggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCg                                                                                                                                   <  1:1848817‑M2/20‑1 (MQ=255)
                   ttgttatctccaacatggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCg                                                                                                                                   >  2:1989994‑M2/130‑149 (MQ=255)
                      ttatctccaacatggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGctc                                                                                                                                >  3:2325872‑M2/127‑149 (MQ=255)
                         aCTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCa                                                                                                                             <  1:640139/149‑1 (MQ=255)
                           tccaacatggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCAtg                                                                                                                           >  1:1434010‑M2/122‑149 (MQ=255)
                           tGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCAtg                                                                                                                           <  1:237947/149‑1 (MQ=255)
                             aTCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCAtgct                                                                                                                         >  1:897813/1‑149 (MQ=255)
                             aTCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCAtgct                                                                                                                         >  2:84006/1‑149 (MQ=255)
                                 aaCTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCAtgc                                                                                                                          <  3:1015665/144‑1 (MQ=255)
                                 aaCTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGctgctg                                                                                                                     <  3:1904553/149‑1 (MQ=255)
                                  tggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTgg                                                                                                                    <  2:1661606‑M2/35‑1 (MQ=255)
                                    tGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGgt                                                                                                                  <  3:722589/149‑1 (MQ=255)
                                    gacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGgt                                                                                                                  >  4:2040918‑M2/113‑149 (MQ=255)
                                     acgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGgtg                                                                                                                 >  4:1816939‑M2/112‑149 (MQ=255)
                                         ggTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAgc                                                                                                             <  1:820448/149‑1 (MQ=255)
                                         aatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAgc                                                                                                             >  3:1726288‑M2/108‑149 (MQ=255)
                                         aatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAgc                                                                                                             <  3:443655‑M2/42‑1 (MQ=255)
                                          atcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAgcg                                                                                                            >  4:793119‑M2/107‑149 (MQ=255)
                                           tcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAgcgc                                                                                                           <  3:1262947‑M2/44‑1 (MQ=255)
                                             gCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCAt                                                                                                         <  4:271889/149‑1 (MQ=255)
                                             aaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCAt                                                                                                         >  2:2242380‑M2/104‑149 (MQ=255)
                                             aaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCAt                                                                                                         >  3:1132256‑M2/104‑149 (MQ=255)
                                              aagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCa                                                                                                                                      <  3:748741‑M2/17‑1 (MQ=255)
                                              aagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCa                                                                                                                                      >  4:748741‑M2/103‑119 (MQ=255)
                                                    tgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTcc                                                                                                  <  3:1207421‑M2/53‑1 (MQ=255)
                                                     gatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCa                                                                                                 <  3:573656‑M2/54‑1 (MQ=255)
                                                     gTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCa                                                                                                 <  4:2459289/149‑1 (MQ=255)
                                                      atcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAg                                                                                                >  1:196582‑M2/95‑149 (MQ=255)
                                                      atcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccataggcgGCGGCAAAATCAATTCATCGCTATCATGCTGCTGGGTGTAGCGCATCACTTCCAg                                                                                                >  1:514148‑M2/95‑149 (MQ=255)
                                                             aaCCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCGTGCTGCTGGGTGTAGCGCATCACTTCCAGTAcgc                                                                                           >  1:1013948/1‑147 (MQ=255)
                                                             aaCCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCGTGCTGCTGGGTGTAGCGCATCACTTCCAGTAcgc                                                                                           <  2:1013948/147‑1 (MQ=255)
                                                               ccccGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCaa                                                                                       <  3:804391/149‑1 (MQ=255)
                                                               ccccGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCaa                                                                                       <  4:2337420/149‑1 (MQ=255)
                                                                            ccgtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGc                                                                          >  2:1128663‑M2/73‑149 (MQ=255)
                                                                             aTCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTa                                                                                                               <  2:1863957/111‑1 (MQ=255)
                                                                              tCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCAc                                                                        <  3:2199360/149‑1 (MQ=255)
                                                                              gtgggtgaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCAc                                                                        <  4:17716‑M2/79‑1 (MQ=255)
                                                                                aagaagTAATTCTTGCCACAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGct                                                                                                                                 >  2:120222/1‑90 (MQ=255)
                                                                                aagaagTAATTCTTGCCACAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGct                                                                                                                                 <  1:120222/90‑1 (MQ=255)
                                                                                    gaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAAt                                                                                                                                         <  2:2301445‑M2/14‑1 (MQ=255)
                                                                                    gaaaccccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAAt                                                                                                                                         >  1:2301445‑M2/65‑78 (MQ=255)
                                                                                     gTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCaa                                                                                                                                          >  3:260287/1‑76 (MQ=255)
                                                                                     gTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGTTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCg                                                                 >  2:954613/1‑149 (MQ=255)
                                                                                     gTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCg                                                                 <  3:1084624/149‑1 (MQ=255)
                                                                                      tAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCgg                                                                <  3:854219/149‑1 (MQ=255)
                                                                                       aaTTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGt                                                               >  2:1581643/1‑149 (MQ=255)
                                                                                        aTTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGtt                                                              >  4:1740721/1‑149 (MQ=255)
                                                                                         ttCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTgg                                                                                                                    <  2:1323061/94‑1 (MQ=255)
                                                                                          ccggttatccgcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCAt                                                                                                                                     >  3:1550488‑M2/59‑76 (MQ=255)
                                                                                           cTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAAc                                                           <  1:1906460/149‑1 (MQ=255)
                                                                                                 ggcgcgAAAAATGGTGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTACCGCCTTc                                                     <  4:1634996/144‑1 (MQ=255)
                                                                                                 gCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTc                                                     >  4:1079837/1‑149 (MQ=255)
                                                                                                    gcatcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCt                                                  <  3:1787371‑M2/101‑1 (MQ=255)
                                                                                                     catcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCtt                                                 <  2:196582‑M2/102‑1 (MQ=255)
                                                                                                     catcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCtt                                                 <  2:2477174‑M2/102‑1 (MQ=255)
                                                                                                      gAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCttt                                                <  4:2153456/149‑1 (MQ=255)
                                                                                                       tcaagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTc                                               <  1:2524026‑M2/104‑1 (MQ=255)
                                                                                                       aaaaaTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTc                                               >  1:1261443/1‑149 (MQ=255)
                                                                                                        caagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCa                                              <  4:701567‑M2/105‑1 (MQ=255)
                                                                                                         aagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCAc                                                                        <  4:1841547‑M2/79‑1 (MQ=255)
                                                                                                         aagaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCAc                                                                        >  3:1841547‑M2/44‑122 (MQ=255)
                                                                                                          agaagtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCgg                                                                >  4:608535‑M2/43‑129 (MQ=255)
                                                                                                              gtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGc                                        <  1:2187012‑M2/111‑1 (MQ=255)
                                                                                                              gtaattcttgccgcagtgaaaaatggcgcccatcggcgGCGGAAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCTCTTTCAGCAAGc                                        >  4:1097996‑M2/39‑149 (MQ=255)
                                                                                                                 attcttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCAc                                     >  1:1324779‑M2/36‑149 (MQ=255)
                                                                                                                     ttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTATCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCGGCAAGCCACtgat                                 <  2:514148‑M2/118‑1 (MQ=255)
                                                                                                                     ttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAAc                                                           <  4:1351004‑M2/92‑1 (MQ=255)
                                                                                                                     ttgccgcagtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAAc                                                           >  3:1351004‑M2/32‑123 (MQ=255)
                                                                                                                         cgcCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTcc                                                                                                  >  4:2297234/1‑80 (MQ=255)
                                                                                                                            cATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGAtgagctga                          <  4:1508389/149‑1 (MQ=255)
                                                                                                                            agtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGAtgagctna                          <  3:67117‑M2/125‑1 (MQ=255)
                                                                                                                             gtgaaaaatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGAtgagctgag                         >  1:94010‑M2/24‑149 (MQ=255)
                                                                                                                                tttttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGcac                      <  4:1611538/149‑1 (MQ=255)
                                                                                                                                   aatggcgcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCGGCAAGCCACTGATGAGCTGAGCACaaa                   >  3:400690‑M2/18‑149 (MQ=255)
                                                                                                                                    tATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACaaaa                  <  1:1364199/149‑1 (MQ=255)
                                                                                                                                       gCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAg               <  2:1428592/149‑1 (MQ=255)
                                                                                                                                         ttCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTcc                                                    >  3:1616438/1‑110 (MQ=255)
                                                                                                                                         ttCCGCCAGCGGCGGCAAAATCAATTCATCGCTCCCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGt             <  1:74896/149‑1 (MQ=255)
                                                                                                                                         gcccatcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGt             >  1:451424‑M2/12‑149 (MQ=255)
                                                                                                                                            catcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGc          >  1:1146681‑M2/9‑149 (MQ=255)
                                                                                                                                              tcggcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCa        <  4:239336‑M2/143‑1 (MQ=255)
                                                                                                                                                 gcgGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTc     >  2:683355‑M2/4‑149 (MQ=255)
                                                                                                                                                    gcggcAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCt  <  4:331157/149‑1 (MQ=255)
                                                                                                                                                    gcggcAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCt  <  4:1001792/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCT  >  NC_000913/2536255‑2536551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.