Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,838,222 A→T 5.4% intergenic (‑117/+32) hydN ← / ← ascG formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/asc operon transcriptional repressor; prpBC operon repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,838,2220AT 5.4% 827.4 / 28.1 241intergenic (‑117/+32)hydN/ascGformate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/asc operon transcriptional repressor; prpBC operon repressor
Reads supporting (aligned to +/- strand):  ref base A (122/106);  new base T (6/7);  total (128/113)
Fisher's exact test for biased strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01

Only 100 of 246 total aligned reads displayed.

TCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAAT  >  NC_000913/2838078‑2838370
                                                                                                                                                |                                                                                                                                                    
tCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACt                                                                                                                                                  >  3:385017/1‑149 (MQ=255)
 cgcgTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTa                                                                                                                                                 <  1:843687/149‑1 (MQ=255)
  gcgTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAg                                                                                                                                                <  4:1286998/149‑1 (MQ=255)
     tCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGa                                                                                                                                             >  3:83378/1‑149 (MQ=255)
        gCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGa                                                                                                                                          <  3:151223/149‑1 (MQ=255)
        gCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGa                                                                                                                                          <  1:1205652/149‑1 (MQ=255)
         cAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGAt                                                                                                                                         <  2:2417738/149‑1 (MQ=255)
         cAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGAt                                                                                                                                         <  2:2220078/149‑1 (MQ=255)
         cAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGAt                                                                                                                                         <  1:1852923/149‑1 (MQ=255)
          aaTGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGa                                                                                                                                             >  1:2281862/1‑144 (MQ=255)
           atgatgAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAg                                                                                                                                                <  2:1116643/140‑1 (MQ=255)
           atgatgAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAg                                                                                                                                                >  1:1116643/1‑140 (MQ=255)
           atgatgAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGa                                                                                                                                       >  3:1216011/1‑149 (MQ=255)
              atgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACAt                                                                                                                                    >  1:1665789/1‑149 (MQ=255)
               tgaAACGATTCATCAAATTCTCCAGCAATAACAGTTAATGCGCCGATACATTCACAAATCTTGCCAGTTTTTACTTTACTGTTATTTAAGGAAGTTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGATGACAtc                                                                                                                                   <  4:261401/149‑2 (MQ=255)
                gaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACAtgt                                                                                                                                  <  3:347304/149‑1 (MQ=255)
                   aCGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtgat                                                                                                                               <  2:421508/149‑1 (MQ=255)
                     ggTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtgatga                                                                                                                             <  2:652329/149‑1 (MQ=255)
                     ggTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtgatga                                                                                                                             >  2:52711/1‑149 (MQ=255)
                      gTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGAc                                                                                                                            >  1:1131739/1‑149 (MQ=255)
                       ttCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACa                                                                                                                           >  1:1038415/1‑149 (MQ=255)
                           tcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtgatga                                                                                                                             <  3:1070262/143‑1 (MQ=255)
                           tcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtgatga                                                                                                                             >  4:1070262/1‑143 (MQ=255)
                           tcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGt                                                                                                                       <  1:621033/149‑1 (MQ=255)
                            caAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGtt                                                                                                                      <  3:2426651/149‑1 (MQ=255)
                            caAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGtt                                                                                                                      <  2:1437933/149‑1 (MQ=255)
                               aTTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGt                                                                                                                                               <  1:1220712/121‑1 (MQ=255)
                                   tcCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATcgcg                                                                                                               >  3:1702635/1‑149 (MQ=255)
                                   tcCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATcgcg                                                                                                               >  3:1328761/1‑149 (MQ=255)
                                     cAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGaa                                                                                                             >  3:1375822/1‑149 (MQ=255)
                                      aGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAg                                                                                                            <  4:1898200/149‑1 (MQ=255)
                                       gCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATc                                                                                                                  <  2:2127639/142‑1 (MQ=255)
                                        cAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGa                                                                                                          <  3:1011230/149‑1 (MQ=255)
                                         aaTGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGGCGATGa                                                                                                                                       >  3:664710/1‑119 (MQ=255)
                                         aaTGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGGCGATGa                                                                                                                                       <  4:664710/119‑1 (MQ=255)
                                            gACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCaa                                                                                                      <  2:2337272/149‑1 (MQ=255)
                                             aCAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAAGAGCAAt                                                                                                     <  2:2324067/149‑1 (MQ=255)
                                               aGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtga                                                                                                   <  3:2257363/149‑1 (MQ=255)
                                               aGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtga                                                                                                   <  2:2172044/149‑1 (MQ=255)
                                                gTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgag                                                                                                  <  3:404183/149‑1 (MQ=255)
                                                gTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgag                                                                                                  <  3:1847998/149‑1 (MQ=255)
                                                 ttAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtga                                                                                                   >  2:2068560/1‑147 (MQ=255)
                                                   aaTGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgagtga                                                                                               <  1:987555/149‑1 (MQ=255)
                                                      gcgcCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTg                                                                                                                                              <  3:511982/99‑1 (MQ=255)
                                                            aTACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgag                                                                                                  >  3:271269/1‑137 (MQ=255)
                                                             tACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGcggcgg                                                                                     >  3:970914/1‑149 (MQ=255)
                                                              aCATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGa                                                                                    >  1:1263900/1‑149 (MQ=255)
                                                               cATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGAt                                                                                   <  4:1954533/149‑1 (MQ=255)
                                                                aTTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCATTGAGTGAGTCGCGGCGGATc                                                                                  >  2:986572/1‑149 (MQ=255)
                                                                aTTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATc                                                                                  >  2:811659/1‑149 (MQ=255)
                                                                 ttCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAAt                                                                                                                         <  4:2318978/109‑1 (MQ=255)
                                                                 ttCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCa                                                                                 >  3:76815/1‑149 (MQ=255)
                                                                    acaAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGtt                                                                              >  3:61232/1‑149 (MQ=255)
                                                                     caAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAAt                                                                                                                         <  1:1761953/105‑1 (MQ=255)
                                                                     caAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTGTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGttt                                                                             >  4:2024339/1‑149 (MQ=255)
                                                                       aaTCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTc                                                                           >  3:1996705/1‑149 (MQ=255)
                                                                              ccAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGaa                                                                    <  4:1943747/149‑1 (MQ=255)
                                                                              ccAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGaa                                                                    <  1:482234/149‑1 (MQ=255)
                                                                              ccAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGaa                                                                    >  1:246919/1‑149 (MQ=255)
                                                                                aGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAgg                                                                  >  1:2129927/1‑149 (MQ=255)
                                                                                  tttttAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGtt                                                                >  1:565055/1‑149 (MQ=255)
                                                                                     ttAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTc                                                             <  1:1810623/149‑1 (MQ=255)
                                                                                        aTTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcggc                                                          >  4:2094835/1‑149 (MQ=255)
                                                                                              tGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTgag                                                    >  1:585251/1‑149 (MQ=255)
                                                                                              tGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTgag                                                    >  1:2018206/1‑149 (MQ=255)
                                                                                                ttATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGaa                                                  >  4:83039/1‑149 (MQ=255)
                                                                                                ttATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGaa                                                  >  2:324811/1‑149 (MQ=255)
                                                                                                   tttAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATc                                               >  4:388809/1‑149 (MQ=255)
                                                                                                     tAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAACTccc                                             <  2:496643/149‑1 (MQ=255)
                                                                                                      aaGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGt                                                                               >  2:480740/1‑114 (MQ=255)
                                                                                                       aGGAAATTAATTTCTGTAATGCATGAAAAACGATGTCATCGACACTa                                                                                                                                                 <  4:159688/47‑1 (MQ=255)
                                                                                                       aGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTa                                                                                                                                                 >  3:159688/1‑47 (MQ=255)
                                                                                                                attTGTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGc                                  >  4:2257246/1‑149 (MQ=255)
                                                                                                                attTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGa                                    >  4:493179/1‑147 (MQ=255)
                                                                                                                  ttCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTCTCGGCGGTGAGAAATCCCCGCCATCGAGCAt                                <  2:1774058/149‑1 (MQ=255)
                                                                                                                   tCTGTAATGCAGGAAAAACGAGGTGAAGGACACGCGTCACCATGACCTGTTATCGCACTGCTTAACGCGAAGCCGCAACGAGTGAGTCGCGGCGGATCAGCATTCCCCCGACGGCTTTCGCCGGTGAGAACTCCCCGCCATGGAGCATa                               <  2:2510123/149‑1 (MQ=255)
                                                                                                                    cTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATaa                              <  1:324811/149‑1 (MQ=255)
                                                                                                                     tGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGATGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATaaa                             >  1:539377/1‑149 (MQ=255)
                                                                                                                       tAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATaaaaa                           >  2:1963523/1‑149 (MQ=255)
                                                                                                                         aTGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTc                                                                           <  1:2098537/99‑1 (MQ=255)
                                                                                                                           gCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAg                       >  4:592610/1‑149 (MQ=255)
                                                                                                                             aGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGcc                     >  1:1877123/1‑149 (MQ=255)
                                                                                                                             aGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGGTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGcc                     >  2:1707487/1‑149 (MQ=255)
                                                                                                                               gAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGt                   >  3:2235913/1‑149 (MQ=255)
                                                                                                                                aaaaaCGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTc                  <  4:1362038/149‑1 (MQ=255)
                                                                                                                                aaaaaCGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTc                  <  1:672110/149‑1 (MQ=255)
                                                                                                                                aaaaaCGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTc                  <  1:2397476/149‑1 (MQ=255)
                                                                                                                                  aaaCGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCa                >  1:1423981/1‑149 (MQ=255)
                                                                                                                                    aCGATTTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGGTCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCaat              >  4:357737/1‑149 (MQ=255)
                                                                                                                                    aCGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAg                       <  4:2433162/140‑1 (MQ=255)
                                                                                                                                       aTGTCATCGTCACTAGTGTCGGTGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCaataat           >  2:781093/1‑149 (MQ=255)
                                                                                                                                        tctCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAAtt          <  3:2024339/147‑1 (MQ=255)
                                                                                                                                           cATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTcc       <  1:1522016/149‑1 (MQ=255)
                                                                                                                                            aTCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCt      >  2:120410/1‑149 (MQ=255)
                                                                                                                                            aTCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTcg                                                                                           >  2:1261472/1‑64 (MQ=255)
                                                                                                                                            aTCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGtt                                                                <  2:565799/91‑1 (MQ=255)
                                                                                                                                               gACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgagt                                                                                                 <  3:2350663/55‑1 (MQ=255)
                                                                                                                                               gACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGaa   <  2:1462349/149‑1 (MQ=255)
                                                                                                                                               gACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGaa   >  4:1473238/1‑149 (MQ=255)
                                                                                                                                                acacTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAat  >  3:1488351/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAAT  >  NC_000913/2838078‑2838370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: