Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,774,222 A→G 5.1% intergenic (+28/‑202) mtlA → / → mtlD mannitol‑specific PTS enzyme: IIA, IIB and IIC components/mannitol‑1‑phosphate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,774,2220AG 5.1% 4647.0 / 142.7 1415intergenic (+28/‑202)mtlA/mtlDmannitol‑specific PTS enzyme: IIA, IIB and IIC components/mannitol‑1‑phosphate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base A (736/603);  new base G (48/25);  total (786/629)
Fisher's exact test for biased strand distribution p-value = 8.98e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 1452 total aligned reads displayed.

CGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTGGGTGAGG  >  NC_000913/3774074‑3774370
                                                                                                                                                    |                                                                                                                                                    
 gAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAg                                                                                                                                                     <  2:155920/149‑1 (MQ=255)
 gAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAg                                                                                                                                                     <  1:1695282/149‑1 (MQ=255)
 gAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAg                                                                                                                                                     >  2:2325214/1‑149 (MQ=255)
   gCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                                                   >  1:55722/1‑149 (MQ=255)
    cacaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAACCCCACCTTCTCCATGTGGAGAGGGTg                                                                                                                                                  <  2:1325246/149‑1 (MQ=255)
     acaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                                                 <  3:1119090/149‑1 (MQ=255)
       aTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgggg                                                                                                                                               >  3:735104/1‑149 (MQ=255)
        ttCAGGTTATCACCAGCCTGACTAATGCACTGGATGATGAGTCTGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTTCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCAGCCTCTCCACATGGAGAAGGTGGGGt                                                                                                                                              >  4:1863529/1‑149 (MQ=255)
         tCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCTTCTCCATGTGGAGAGGGTGGGAtt                                                                                                                                             <  3:89311/149‑1 (MQ=255)
          cAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTa                                                                                                                                            >  2:1661441/1‑149 (MQ=255)
            ggTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTGAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAAt                                                                                                                                          <  3:1415019/149‑1 (MQ=255)
             gTTATCACCCGCCCTACCAATCCACTGGCTGATGAGTGCGACATCGAGCGTATGGCACACACCACCAGCGTAGATGCAGTGCTGGAATTCATGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGATCATTTGGGGTGAAtt                                                                                                                                         >  2:24765/1‑149 (MQ=255)
             gTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAAtt                                                                                                                                         >  3:1053501/1‑149 (MQ=255)
               tATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGc                                                                                                                                       <  2:1029310/149‑1 (MQ=255)
                aTCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGcc                                                                                                                                      <  1:2248341/149‑1 (MQ=255)
                   acgaGCCTGACCGATGCACTGAATGATGAGTCCGTCATAGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGATGGGGTTAATTGCCTGa                                                                                                                                   >  2:569736/4‑149 (MQ=255)
                   aCCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGa                                                                                                                                   >  1:792613/1‑149 (MQ=255)
                    ccAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                                                   <  3:977637/132‑1 (MQ=255)
                       gCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATgcg                                                                                                                               <  1:2329895/149‑1 (MQ=255)
                         cTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCt                                                                                                                             >  3:577697/1‑149 (MQ=255)
                              cAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCt                                                                                                                        >  2:2250984/1‑149 (MQ=255)
                                    aCTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGc                                                                                                                                       >  3:2352313/1‑128 (MQ=255)
                                    aCTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAg                                                                                                                  >  1:1973052/1‑149 (MQ=255)
                                    aCTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAg                                                                                                                  <  2:1973052/149‑1 (MQ=255)
                                     cTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGTTTATCAgg                                                                                                                 >  3:1332735/1‑149 (MQ=255)
                                     cTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAgg                                                                                                                 <  1:1343347/149‑1 (MQ=255)
                                      tGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGc                                                                                                                <  4:680201/149‑1 (MQ=255)
                                       ggATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGcc                                                                                                               >  2:218624/1‑149 (MQ=255)
                                        gatgatGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAg                                                                                                                                                     >  4:879006/1‑110 (MQ=255)
                                        gatgatGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATgcgc                                                                                                                              <  2:688221/133‑1 (MQ=255)
                                           gatgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGc                                                                                                                                       >  2:1236479/1‑121 (MQ=255)
                                           gatgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACa                                                                                                           <  3:1987351/149‑1 (MQ=255)
                                             tgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAgg                                                                                                         >  4:2254175/1‑149 (MQ=255)
                                               aGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGAt                                                                                                       <  1:2042782/149‑1 (MQ=255)
                                                gTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATg                                                                                                      >  4:315574/1‑149 (MQ=255)
                                                    gTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTggg                                                                                                                                                <  4:1037383/103‑1 (MQ=255)
                                                       aTCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATcaca                                                                                               >  3:1242739/1‑149 (MQ=255)
                                                        tCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACaa                                                                                              <  1:1904613/149‑1 (MQ=255)
                                                          gAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAAtt                                                                                            >  1:1859695/1‑149 (MQ=255)
                                                          gAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAAtt                                                                                            >  4:558929/1‑149 (MQ=255)
                                                             cGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATttgt                                                                                         <  4:19853/149‑1 (MQ=255)
                                                              gTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATttgtt                                                                                        <  4:764768/149‑1 (MQ=255)
                                                               tCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATttgttg                                                                                       <  3:2274078/149‑1 (MQ=255)
                                                                 tGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGaa                                                                                     >  2:1893773/1‑149 (MQ=255)
                                                                   gCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAAtt                                                                                   >  1:2517838/1‑149 (MQ=255)
                                                                   gCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAAtt                                                                                   <  1:634885/149‑1 (MQ=255)
                                                                     acacacCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATGCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCAGCACAATTTGATGAATTTg                                                                                 >  3:2122386/1‑149 (MQ=255)
                                                                     acacacCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                                                   >  2:387323/1‑83 (MQ=255)
                                                                     acacacCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGACGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTg                                                                                 >  3:1520247/1‑149 (MQ=255)
                                                                      cacacCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGc                                                                                <  4:2347627/149‑1 (MQ=255)
                                                                           caccaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGtt                                                                           >  1:1242424/1‑149 (MQ=255)
                                                                             ccaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCt                                                                         >  3:2222356/1‑149 (MQ=255)
                                                                              caGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCtt                                                                        >  1:948922/1‑149 (MQ=255)
                                                                              caGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCtt                                                                        <  3:1638125/149‑1 (MQ=255)
                                                                                gCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGt                                                                      <  2:1778099/149‑1 (MQ=255)
                                                                                     gATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGcc                                                                 >  1:1111951/1‑149 (MQ=255)
                                                                                      aTGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTAt                                                                                                                     >  1:2399273/1‑96 (MQ=255)
                                                                                            tGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGc                                                                                                     <  2:514869/106‑1 (MQ=255)
                                                                                             gCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGACGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAgg                                                         >  4:1626737/1‑149 (MQ=255)
                                                                                                ggAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGgcgc                                                      <  4:1767693/149‑1 (MQ=255)
                                                                                                     tgctgGCAGGTCGTAAGTAATCAAATCCCACCCTCTCCACATGGAGAAGGTggg                                                                                                                                                <  4:1523400/54‑1 (MQ=255)
                                                                                                              gTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGAt                                                                                                                                  <  2:2389100/59‑1 (MQ=255)
                                                                                                              gTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCg                                        <  3:2030137/149‑1 (MQ=255)
                                                                                                                 gtaagtaaTCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGgcg                                     >  1:215271/1‑149 (MQ=255)
                                                                                                                  taagtaaTCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATttgtt                                                                                        >  3:336909/1‑97 (MQ=255)
                                                                                                                     gtaaTCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGc                                 >  2:903181/1‑149 (MQ=255)
                                                                                                                     gtaaTCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGc                                 >  1:142784/1‑149 (MQ=255)
                                                                                                                       aatccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCtt                                                                        <  4:1342543/108‑1 (MQ=255)
                                                                                                                         tccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCAt                                           >  4:1422284/1‑135 (MQ=255)
                                                                                                                         tccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCct                             >  4:473122/1‑149 (MQ=255)
                                                                                                                         tccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCct                             >  4:2076515/1‑149 (MQ=255)
                                                                                                                              tccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTctcct                        <  2:146034/149‑1 (MQ=255)
                                                                                                                               cccACCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTctcctc                       >  3:1188393/1‑149 (MQ=37)
                                                                                                                                ccACCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTc                                                                          >  1:1766173/1‑97 (MQ=37)
                                                                                                                                ccACCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGcc                                <  3:2460532/139‑1 (MQ=25)
                                                                                                                                ccACCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGcc                                >  4:2460532/1‑139 (MQ=37)
                                                                                                                                 cACCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAg                                                          <  3:427234/112‑1 (MQ=37)
                                                                                                                                 cACCTTCTCCATGTGGAGAGGGTGGGATTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCAc                     >  2:983581/1‑149 (MQ=25)
                                                                                                                                 cACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCAc                     >  1:7199/1‑149 (MQ=255)
                                                                                                                                   cccTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGG                   >  3:667138‑M1/1‑149 (MQ=255)
                                                                                                                                     ctctCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAG                 >  4:2194987‑M1/1‑149 (MQ=255)
                                                                                                                                     ctctCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAG                 >  3:1893176‑M1/1‑149 (MQ=255)
                                                                                                                                      tctcCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGA                <  2:798991‑M1/149‑1 (MQ=255)
                                                                                                                                       ctcCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAG               <  4:1239860‑M1/149‑1 (MQ=255)
                                                                                                                                        tcCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGG              <  2:1144380‑M1/149‑1 (MQ=255)
                                                                                                                                         ccACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGG             >  1:900699‑M1/1‑149 (MQ=255)
                                                                                                                                           acaTGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGtt           >  1:1556442/1‑149 (MQ=255)
                                                                                                                                           acaTGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGtt           >  1:1002339/1‑149 (MQ=255)
                                                                                                                                             aTGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTg         <  4:1124091/149‑1 (MQ=255)
                                                                                                                                             aTGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTg         <  2:310657/149‑1 (MQ=255)
                                                                                                                                              tGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTgg        <  3:809924/149‑1 (MQ=255)
                                                                                                                                              tGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTgg        >  1:1650206/1‑149 (MQ=255)
                                                                                                                                              tGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTgg        >  2:2063345/1‑149 (MQ=255)
                                                                                                                                              tGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTgg        <  1:1184638/149‑1 (MQ=255)
                                                                                                                                              tGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTgg        <  3:1750240/149‑1 (MQ=255)
                                                                                                                                              tGGAGAAGGTGGGGTTAATCGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTgg        <  4:1692029/149‑1 (MQ=255)
                                                                                                                                               ggAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTggg       <  3:1834416/149‑1 (MQ=255)
                                                                                                                                                 agaAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTGGGTg     >  4:378756/1‑149 (MQ=255)
                                                                                                                                                   aaGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTGGGTGAg   <  1:323846/149‑1 (MQ=255)
                                                                                                                                                   aaGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTGGGTGAg   <  1:466719/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTGGGTGAGG  >  NC_000913/3774074‑3774370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: