Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,963,416 G→T 5.4% L272M (CTG→ATG) ‡ gpp ← guanosine pentaphosphatase/exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,963,4160GT 5.4% 848.6 / 24.2 240L272M (CTG→ATG) ‡gppguanosine pentaphosphatase/exopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base G (104/123);  new base T (4/9);  total (108/132)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

Only 100 of 242 total aligned reads displayed.

ATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATC  >  NC_000913/3963268‑3963564
                                                                                                                                                    |                                                                                                                                                    
      cggcgTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGc                                                                                                                                                <  3:1198998/149‑1 (MQ=255)
      cggcgTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGc                                                                                                                                                <  1:716289/149‑1 (MQ=255)
       ggcgTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGcc                                                                                                                                               >  1:1838614/1‑149 (MQ=255)
        gcgTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCa                                                                                                                                              <  1:2125350/149‑1 (MQ=255)
         cgTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCCTGATCGCCat                                                                                                                                             <  1:1617729/149‑2 (MQ=255)
           ttGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCCTGATCGCCattt                                                                                                                                           <  4:1116528/149‑4 (MQ=255)
              aaTATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCCTGATCGCCattttta                                                                                                                                        <  4:36331/149‑7 (MQ=255)
                tatTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTc                                                                                                                                      <  4:909305/149‑1 (MQ=255)
                 atTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCg                                                                                                                                     >  1:1712776/1‑149 (MQ=255)
                  ttACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCgg                                                                                                                                    <  1:2301148/149‑1 (MQ=255)
                    aCGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGa                                                                                                                                  <  3:2396387/149‑1 (MQ=255)
                       cAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAaca                                                                                                                               <  3:1970029/149‑1 (MQ=255)
                       cAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAaca                                                                                                                               <  3:2445070/149‑1 (MQ=255)
                           gTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTaa                                                                                                                           <  2:1446065/149‑1 (MQ=255)
                            tACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAAc                                                                                                                          <  2:436501/149‑1 (MQ=255)
                            tACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAAc                                                                                                                          <  2:544870/149‑1 (MQ=255)
                            tACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCAGGAACACTAAc                                                                                                                          <  2:747680/149‑1 (MQ=255)
                                    cGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTc                                                                                                                  <  3:595489/149‑1 (MQ=255)
                                     gAATATCCTGCTCGACGGCCAGATGCAACATGTCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTcc                                                                                                                 <  3:556927/149‑1 (MQ=255)
                                     gAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTcc                                                                                                                 >  2:827885/1‑149 (MQ=255)
                                        tatCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGc                                                                                                              >  1:829958/1‑149 (MQ=255)
                                        tatCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGc                                                                                                              <  3:658571/149‑1 (MQ=255)
                                        tatCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGc                                                                                                              <  4:2417756/149‑1 (MQ=255)
                                          tCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGt                                                                                                            <  4:2458403/149‑1 (MQ=255)
                                           ccTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTc                                                                                                           >  3:332349/1‑149 (MQ=255)
                                                tCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCggg                                                                                                                                   <  2:1657506/120‑1 (MQ=255)
                                                tCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGccc                                                                                                      >  1:635748/1‑149 (MQ=255)
                                                  gACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGt                                                                                                    <  4:414984/149‑1 (MQ=255)
                                                   aCGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCTCTCGGGAACACCAACGCACGTTCCAGCGTCAGCCCGTc                                                                                                   <  3:1540986/149‑1 (MQ=255)
                                                       ccAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGGCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTACCAGCGTCAGCCCGTCAAtc                                                                                               <  2:377193/149‑1 (MQ=255)
                                                       ccAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCCTGATCGCCa                                                                                                                                              >  4:1928746/1‑102 (MQ=255)
                                                       ccAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCCTGATCGCCa                                                                                                                                              <  3:1928746/102‑1 (MQ=255)
                                                       ccAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAAtc                                                                                               <  3:1110666/149‑1 (MQ=255)
                                                         aGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAAtctc                                                                                             <  4:1539536/149‑1 (MQ=255)
                                                           aTGCAACATGCCGTAGACCAGGTCTTCACGCAGCGGACCGCCCGACAGGGTCATACTCTGAATATTCAGTTCGGTAATAATGGCGATCAGGATCGCCAGACCACACGGGAACACTAACGCAGGTTCCAGCGCCAGCCCGTCAATCTACa                                                                                           <  4:1093/149‑1 (MQ=255)
                                                           aTGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCa                                                                                           <  1:486839/149‑1 (MQ=255)
                                                           aTGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCa                                                                                           <  1:2379563/149‑1 (MQ=255)
                                                            tGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAg                                                                                          >  2:2019147/1‑149 (MQ=255)
                                                               aaCATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATc                                                                                                                                                  <  2:2147603/90‑1 (MQ=255)
                                                               aaCATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTtc                                                                                       >  1:253843/1‑149 (MQ=255)
                                                                   tGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCCCGTTCCAGCGTCAGCCCGTCAATCTCCAGTtcctcc                                                                                   <  4:621509/149‑1 (MQ=255)
                                                                      cGTAGACCAGGCTTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTtcc                                                                                      >  4:2348096/1‑143 (MQ=255)
                                                                      cGTAGACCAGGCTTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGATCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTtcc                                                                                      <  3:2348096/143‑1 (MQ=255)
                                                                      cGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCGGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGc                                                                                >  2:1140426/1‑149 (MQ=255)
                                                                       gTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGcc                                                                               <  1:445520/149‑1 (MQ=255)
                                                                             cAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGc                                                                         >  4:1315422/1‑149 (MQ=255)
                                                                             cAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGc                                                                         >  2:1581294/1‑149 (MQ=255)
                                                                                 ccTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatg                                                                     >  1:1282894/1‑149 (MQ=255)
                                                                                   ttCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatgaa                                                                   <  4:1600456/149‑1 (MQ=255)
                                                                                   ttCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatgaa                                                                   >  3:1768919/1‑149 (MQ=255)
                                                                                      aCGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATgg                                                                <  2:253843/149‑1 (MQ=255)
                                                                                      aCGCAGCGCACCGCCCGCCAGGGCCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATgg                                                                >  2:1009041/1‑149 (MQ=255)
                                                                                       cGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGc                                                               >  1:2114259/1‑149 (MQ=255)
                                                                                           gcgcACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGt                                                                                                    <  3:1268666/108‑1 (MQ=255)
                                                                                           gcgcACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGc                                                           >  3:812395/1‑149 (MQ=255)
                                                                                                ccgcccgcCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGttt                                                      <  2:1515064/149‑1 (MQ=255)
                                                                                                   cccgccAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCaa                                                   <  4:2277876/149‑1 (MQ=255)
                                                                                                     cgccAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAAtt                                                 <  1:581042/149‑1 (MQ=255)
                                                                                                     cgccAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAAtt                                                 >  1:1674981/1‑149 (MQ=255)
                                                                                                       ccAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCGAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTCCAATtgc                                               <  4:2104538/149‑1 (MQ=255)
                                                                                                       ccAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTTGCTGTTTCAATtgc                                               <  3:187824/149‑1 (MQ=255)
                                                                                                       ccAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATtgc                                               >  2:2320645/1‑149 (MQ=255)
                                                                                                            gccATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCaa                                          <  1:1009041/147‑1 (MQ=255)
                                                                                                                tACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACttt                                      <  2:2240253/149‑1 (MQ=255)
                                                                                                                   acTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTcc                                   <  4:1344117/149‑1 (MQ=255)
                                                                                                                   acTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTcc                                   >  3:725915/1‑149 (MQ=255)
                                                                                                                    cTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCt                                                          <  2:565469/125‑1 (MQ=255)
                                                                                                                    cTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCa                                  >  4:813031/1‑149 (MQ=255)
                                                                                                                    cTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCa                                  >  3:460532/1‑149 (MQ=255)
                                                                                                                    cTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCa                                  >  3:1096309/1‑149 (MQ=255)
                                                                                                                    cTGAATATTCAGTTCGGTAAAAATGGCGATCAGGACCGCAAAACCACTGGGAACCCCTAACGAACTTTCCACCGTAAGCCCGTAAACCCCCAGTTCCTCCACCCGCCCCCAATGAATGCCCCGCTGTTCCAATGGTTCCACCTTTTccg                                  >  4:32824/1‑148 (MQ=255)
                                                                                                                     tGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAAtc                                                                                               >  2:2267127/1‑87 (MQ=255)
                                                                                                                     tGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTtcctcc                                                                                   <  4:1008425/99‑1 (MQ=255)
                                                                                                                     tGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatgaa                                                                   >  1:2369773/1‑115 (MQ=255)
                                                                                                                     tGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAg                                 <  2:651405/149‑1 (MQ=255)
                                                                                                                     tGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAg                                 <  4:1047722/149‑1 (MQ=255)
                                                                                                                      gAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACttt                                      >  1:1166698/1‑143 (MQ=255)
                                                                                                                      gAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAgg                                <  1:2061365/149‑1 (MQ=255)
                                                                                                                       aaTATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAggg                               >  4:587485/1‑149 (MQ=255)
                                                                                                                       aaTATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAggg                               >  3:1503504/1‑149 (MQ=255)
                                                                                                                                ttCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGtt                      <  4:1204861/149‑1 (MQ=255)
                                                                                                                                 tCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTc                     <  1:1506772/149‑1 (MQ=255)
                                                                                                                                 tCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTc                     <  1:1462468/149‑1 (MQ=255)
                                                                                                                                  cGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTca                    <  1:79526/149‑1 (MQ=255)
                                                                                                                                    gTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTcatc                  >  1:156617/1‑149 (MQ=255)
                                                                                                                                     tAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTcatcc                 <  1:1269554/149‑1 (MQ=255)
                                                                                                                                     tAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTATTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTcatcc                 <  4:1435342/149‑1 (MQ=255)
                                                                                                                                        aaaTGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTcatccatc              <  2:2377571/149‑1 (MQ=255)
                                                                                                                                          ctGGCGATCCTGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAggg                               >  3:935762/2‑130 (MQ=255)
                                                                                                                                          aTGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTc                     >  3:1376365/1‑140 (MQ=255)
                                                                                                                                           tGGCGATCCTGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGc                                                               >  3:1321268/1‑97 (MQ=255)
                                                                                                                                           tGGCGATCCTGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATcccc           >  3:2263686/1‑149 (MQ=255)
                                                                                                                                           tGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATcccc           <  4:1983021/149‑1 (MQ=255)
                                                                                                                                           tGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATcccc           >  3:1698300/1‑149 (MQ=255)
                                                                                                                                            ggCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATGCCCt          <  2:1278187/149‑1 (MQ=255)
                                                                                                                                              cGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCtgt        <  1:2246042/149‑1 (MQ=255)
                                                                                                                                              cGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCtgt        <  3:286528/149‑1 (MQ=255)
                                                                                                                                               gATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGt                       >  1:190442/1‑133 (MQ=255)
                                                                                                                                                aTCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTc                     <  4:1394026/134‑1 (MQ=255)
                                                                                                                                                aTCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTc                     >  3:1394026/1‑134 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATC  >  NC_000913/3963268‑3963564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: