Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 603,395 C→G 6.0% intergenic (+60/+21) pheP → / ← ybdG phenylalanine transporter/mechanosensitive channel protein, miniconductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913603,3950CG 6.0% 1211.9 / 51.4 346intergenic (+60/+21)pheP/ybdGphenylalanine transporter/mechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base C (169/155);  new base G (10/11);  total (180/166)
Fisher's exact test for biased strand distribution p-value = 8.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

Only 100 of 347 total aligned reads displayed.

CGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCATA  >  NC_000913/603247‑603541
                                                                                                                                                    |                                                                                                                                                  
   tggatgATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAGCCGCTCTCATCCATTCGAt                                                                                                                                                 <  2:2114764/149‑1 (MQ=255)
   tggatgATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGAt                                                                                                                                                 <  3:1520110/149‑1 (MQ=255)
     gatgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATga                                                                                                                                               <  2:1331824/149‑1 (MQ=255)
          tatGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCGAATGGATGAGAGCg                                                                                                                                          <  3:1158377/149‑1 (MQ=255)
           atGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCgg                                                                                                                                         <  2:888628/149‑1 (MQ=255)
            tGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGt                                                                                                                                        >  3:1459815/1‑149 (MQ=255)
            tGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGt                                                                                                                                        >  2:783660/1‑149 (MQ=255)
               gcTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCGAATGGATGAGAGCGGtttc                                                                                                                                     <  1:2484098/149‑2 (MQ=255)
                cTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGttttt                                                                                                                                    >  3:783816/1‑149 (MQ=255)
                cTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGttttt                                                                                                                                    <  4:2050113/149‑1 (MQ=255)
                cTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGttttt                                                                                                                                    <  4:512414/149‑1 (MQ=255)
                cTTGTCAGCGAGCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGttttt                                                                                                                                    >  3:1951085/1‑149 (MQ=255)
                 ttGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGtttttt                                                                                                                                   <  4:1186696/149‑1 (MQ=255)
                 ttGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGtttttt                                                                                                                                   >  4:1733805/1‑149 (MQ=255)
                 ttGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGtttttt                                                                                                                                   >  2:1785165/1‑149 (MQ=255)
                    tCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGGTTTTTTaa                                                                                                                                >  2:1177902/1‑149 (MQ=255)
                    tCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTCTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAATCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTaa                                                                                                                                <  1:146687/149‑1 (MQ=255)
                       gCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTa                                                                                                                             <  4:763322/149‑1 (MQ=255)
                       gCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTa                                                                                                                             >  4:426530/1‑149 (MQ=255)
                        cGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTAc                                                                                                                            <  2:1057993/149‑1 (MQ=255)
                                 tgcCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGCCAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAAt                                                                                                                   <  2:366225/149‑1 (MQ=255)
                                 tgcCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAAt                                                                                                                   <  1:1982573/149‑1 (MQ=255)
                                 tgcCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAAt                                                                                                                   <  4:234199/149‑1 (MQ=255)
                                      gtgtGGATTGTATTCCTGCTTATGGCATTTAAAACGCTGCGCCGGAAATAAGGCATTCAAGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGAGGAGAGCGGTTTTTTTAGTTACTGCTTAAATGCAcc                                                                                                              >  2:43838/1‑149 (MQ=255)
                                        gtgGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCg                                                                                                            >  3:2284935/1‑149 (MQ=255)
                                           gATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCa                                                                                                         >  4:906849/1‑149 (MQ=255)
                                                tATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGAt                                                                                                                                                 >  4:1393358/1‑104 (MQ=255)
                                                tATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGtttttt                                                                                                                                   >  3:1671843/1‑118 (MQ=255)
                                                 aTTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATgaga                                                                                                                                             <  3:42952/107‑1 (MQ=255)
                                                  ttCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCg                                                                                                  >  4:551071/1‑149 (MQ=255)
                                                     cTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAat                                                                                               >  3:669381/1‑149 (MQ=255)
                                                      tGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCGAATGGATGAGAGCgg                                                                                                                                         <  1:1207196/106‑1 (MQ=39)
                                                      tGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGtttttt                                                                                                                                   <  1:2298599/112‑1 (MQ=255)
                                                      tGTTTATGGCATTTAAAACACTGCGTCGGAAATAAGACATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGATTTTTTTAATTACTGCTTAAATGCACCACCCAGAGAGCGAata                                                                                              <  4:730383/149‑1 (MQ=255)
                                                       gTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAatat                                                                                             <  1:61208/149‑1 (MQ=255)
                                                        tttATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATc                                                                                            >  3:628705/1‑149 (MQ=255)
                                                           aTGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCAtt                                                                                         <  3:2175442/149‑1 (MQ=255)
                                                           aTGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCAtt                                                                                         <  3:1348209/149‑1 (MQ=255)
                                                                 tttAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGt                                                                                   >  4:845529/1‑149 (MQ=255)
                                                                      aaCGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCg                                                                              >  2:553175/1‑149 (MQ=255)
                                                                       aCGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGa                                                                             <  3:551071/149‑1 (MQ=255)
                                                                          cTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACtg                                                                          <  1:177726/149‑1 (MQ=255)
                                                                               tCGGAAATAAGGCATTCACGCTCCATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCGAATGGATGAGAGc                                                                                                                                           >  1:1529163/1‑79 (MQ=37)
                                                                               tCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGaa                                                                     <  3:1299791/149‑1 (MQ=255)
                                                                               tCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGaa                                                                     >  4:2448135/1‑149 (MQ=255)
                                                                               tCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGaa                                                                     <  1:1731585/149‑1 (MQ=255)
                                                                                 ggAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGTTTGGCGACTGATGAAGt                                                                   >  2:270455/1‑149 (MQ=255)
                                                                                   aaaTAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCg                                                                 >  1:1006976/1‑149 (MQ=255)
                                                                                     aTAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCa                                                               <  1:1588810/149‑1 (MQ=255)
                                                                                          gCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCa                                                               >  3:1876447/1‑144 (MQ=255)
                                                                                            aTTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCaaa                                                        >  4:35348/1‑149 (MQ=255)
                                                                                                aCGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGaa                                                                     >  1:1414419/1‑132 (MQ=255)
                                                                                                aCGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAActct                                                    >  3:222351/1‑149 (MQ=255)
                                                                                                  gCTACCTCCGCCAAAACGTTGTCAACCATCCGAATCGGCTCTAAACCATTCGATGATAGCGGTTTTTATAATTACTGCTTAAATGCACCCGCCAGAGAGCAAATCTCCTTGCCGGTTGGTGAATGATGAAGTCGCAGACCAATCTCTTc                                                  <  2:2359034/149‑1 (MQ=255)
                                                                                                      cATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACa                                              >  2:1869815/1‑149 (MQ=255)
                                                                                                       aTCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACaa                                             >  3:176609/1‑149 (MQ=255)
                                                                                                         ccGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATc                                           >  3:475173/1‑149 (MQ=255)
                                                                                                          cGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATc                                           >  2:743382/1‑148 (MQ=255)
                                                                                                          cGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCg                                          >  2:1525368/1‑149 (MQ=255)
                                                                                                           gACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGc                                         >  3:862345/1‑149 (MQ=255)
                                                                                                           gACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATACACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGc                                         <  1:590430/149‑1 (MQ=255)
                                                                                                              aaaaCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCaaa                                      <  2:1267019/149‑1 (MQ=255)
                                                                                                               aaaCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGtt                                                                                  <  3:761949/104‑1 (MQ=255)
                                                                                                                aaCGATTTCAACCATCCGAAAGCGCTCTCATCCATTCGTTTAGTGCGGTTTTTTTAATTACTGCTTAGATGCACCCGCCAGAGAGCGAATGCTATTGACGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTACGACAATCGCAAata                                    >  2:2288647/1‑149 (MQ=255)
                                                                                                                aaCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAata                                    >  3:949805/1‑149 (MQ=255)
                                                                                                                aaCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAata                                    >  1:996584/1‑149 (MQ=255)
                                                                                                                   gATGTCAAACATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATAtgt                                 <  2:2056128/149‑1 (MQ=255)
                                                                                                                     tGTCAACCATCCGAAACCGCTCTCATCCATGCGAGGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGa                               <  1:442460/149‑1 (MQ=255)
                                                                                                                      gTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGAt                              <  2:1698186/149‑1 (MQ=255)
                                                                                                                      gTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGAt                              <  1:472648/149‑1 (MQ=255)
                                                                                                                       tCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACtgat                                                                        >  1:2110975/1‑106 (MQ=255)
                                                                                                                       tCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATc                             >  4:2261710/1‑149 (MQ=255)
                                                                                                                        cAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCg                            <  3:2418491/149‑1 (MQ=255)
                                                                                                                         aaCCATCCGAAACCGCTCTCATCCATTCGTTGAGAGCGGTTTTTTTAATTACTGCTTAAATGc                                                                                                                 >  3:2018490/1‑63 (MQ=255)
                                                                                                                         aaCCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGc                                                                                                                 <  4:2018490/63‑1 (MQ=255)
                                                                                                                         aaCCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGa                           >  1:1128782/1‑149 (MQ=255)
                                                                                                                          aCCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGaa                          <  3:101338/149‑1 (MQ=255)
                                                                                                                           ccATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATc                                                                                            >  3:2475647/1‑82 (MQ=255)
                                                                                                                           ccATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAat                         <  4:222351/149‑1 (MQ=255)
                                                                                                                             aTCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAatat                       <  1:268933/149‑1 (MQ=255)
                                                                                                                               ccGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAatatat                     <  2:972912/149‑1 (MQ=255)
                                                                                                                               aaaaaaCCGCTCTCATCGAATGGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAatatat                     >  2:1479817/4‑149 (MQ=255)
                                                                                                                                 gAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAat                         >  1:907771/1‑143 (MQ=255)
                                                                                                                                 gAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCa                   >  4:1671501/1‑149 (MQ=255)
                                                                                                                                 gAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCa                   <  1:1920189/149‑1 (MQ=255)
                                                                                                                                 gAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCa                   >  4:1328098/1‑149 (MQ=255)
                                                                                                                                       gCTCTCATCGAATGGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGa             >  2:1839091/1‑149 (MQ=255)
                                                                                                                                        ctctCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGaa            >  1:828784/1‑149 (MQ=255)
                                                                                                                                         tctcATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATc                             >  1:367496/1‑131 (MQ=255)
                                                                                                                                         tctcATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAAt           >  4:1071580/1‑149 (MQ=255)
                                                                                                                                         tctcATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAAt           <  4:1827650/149‑1 (MQ=255)
                                                                                                                                           tcATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGc         >  1:714724/1‑149 (MQ=255)
                                                                                                                                             aTCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTTGACAATCGCAAATATGTGATCGAATATATCAGc                 <  3:1571946/139‑1 (MQ=255)
                                                                                                                                             aTCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCtt       <  4:687323/149‑1 (MQ=255)
                                                                                                                                             aTCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCAGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATGAGc                 >  4:1571946/1‑139 (MQ=255)
                                                                                                                                               ccATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTc     <  1:2366318/149‑1 (MQ=255)
                                                                                                                                               ccATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTc     <  4:628705/149‑1 (MQ=255)
                                                                                                                                               ccATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATACACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTc     >  4:2141472/1‑149 (MQ=255)
                                                                                                                                               ccATTCAATTAGAGCGGTTTTTTTAAGGACTGATTAAATGCACTAGCCAACGAGCGAATATCAATGACGGCTGGCAACTGATGAAGTCGCAGAACAAGCTCTTCGACAATCGCAAATAAGTGATCGAACATATAAGCCTGAATACTTTc     <  2:2463627/149‑1 (MQ=255)
                                                                                                                                                  ttCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCata  >  1:1835524/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCATA  >  NC_000913/603247‑603541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: