Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,113,544 G→A 5.9% intergenic (+138/‑35) opgH → / → yceK OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist/outer membrane integrity lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,113,5440GA 5.9% 1063.2 / 34.9 323intergenic (+138/‑35)opgH/yceKOPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist/outer membrane integrity lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (151/152);  new base A (6/13);  total (157/166)
Fisher's exact test for biased strand distribution p-value = 1.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.99e-01

Only 100 of 323 total aligned reads displayed.

TTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCCCGGTGTGC  >  NC_000913/1113397‑1113690
                                                                                                                                                   |                                                                                                                                                  
ttCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGt                                                                                                                                                   >  4:2083504/1‑149 (MQ=255)
ttCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGt                                                                                                                                                   >  4:1855704/1‑149 (MQ=255)
    cAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATg                                                                                                                                               >  2:1642411/1‑149 (MQ=255)
     aaTAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGc                                                                                                                                              >  1:1009937/1‑149 (MQ=255)
      aTAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTTCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGcc                                                                                                                                             <  4:347777/149‑1 (MQ=255)
       tAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCg                                                                                                                                            >  3:189388/1‑149 (MQ=255)
        aaaaaCGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCgg                                                                                                                                           >  3:2361588/1‑149 (MQ=255)
          caaCGTAGTTGCCAGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGTTa                                                                                                                                         >  4:1605618/2‑149 (MQ=255)
          aagcGTAGTTGACTGATGCGGTACGCTTATCAGGCCTACATCGTTTCTGCAATTTATTGATTTTGCAACACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAACAATAACGAGCCAATACAAATACCGGCATAACGCCGGTa                                                                                                                                         <  4:1725090/146‑1 (MQ=21)
          aaaCGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTa                                                                                                                                         <  2:1961602/149‑1 (MQ=255)
          aaaCGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGCATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTa                                                                                                                                         <  2:1129869/149‑1 (MQ=255)
           aaCGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAt                                                                                                                                        >  1:2040472/1‑149 (MQ=255)
             cGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAttt                                                                                                                                      <  4:906273/149‑1 (MQ=255)
                  ttGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTa                                                                                                                                 <  3:1972328/149‑1 (MQ=255)
                       tGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGaaa                                                                                                                            >  2:1912452/1‑149 (MQ=255)
                       tGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGaaa                                                                                                                            <  3:263095/149‑1 (MQ=255)
                          tGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAagag                                                                                                                         <  3:83625/149‑1 (MQ=255)
                          tGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAagag                                                                                                                         <  4:1094926/149‑1 (MQ=255)
                          tGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAagag                                                                                                                         <  4:845172/149‑1 (MQ=255)
                           gcgcTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAgg                                                                                                                        >  3:2120727/1‑149 (MQ=255)
                             gcTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAttttt                                                                                                                                    >  2:34502/1‑135 (MQ=255)
                             gcTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAttttt                                                                                                                                    <  1:34502/135‑1 (MQ=255)
                             gcTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTa                                                                                                                                 <  2:2285553/138‑1 (MQ=255)
                             gcTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTa                                                                                                                                 >  1:2285553/1‑138 (MQ=255)
                                   cTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAAt                                                                                                                <  3:620214/149‑1 (MQ=255)
                                    ttATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAaga                                                                                                                          <  4:1947449/138‑1 (MQ=255)
                                    ttATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATg                                                                                                               >  2:1594032/1‑149 (MQ=255)
                                    ttATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATg                                                                                                               <  1:2116900/149‑1 (MQ=255)
                                    ttATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATg                                                                                                               >  2:1203268/1‑149 (MQ=255)
                                    ttATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATg                                                                                                               >  3:65919/1‑149 (MQ=255)
                                     tATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGc                                                                                                              <  3:1783615/149‑1 (MQ=255)
                                     tATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGc                                                                                                              <  2:2342985/149‑1 (MQ=255)
                                       tCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGt                                                                                                            <  1:2188462/149‑1 (MQ=255)
                                        cAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGtt                                                                                                           <  2:945405/149‑1 (MQ=255)
                                          ggCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTa                                                                                                         >  1:889549/1‑149 (MQ=255)
                                          ggCCTACATCGGTCCTGCAATTTATTGATTTTGCAAGACTTGGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATAAAATGCGTTTa                                                                                                         >  4:2423023/1‑149 (MQ=255)
                                           gCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTaa                                                                                                        <  1:1634218/149‑1 (MQ=255)
                                           gCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTATGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTaa                                                                                                        <  4:1774212/149‑1 (MQ=255)
                                            ccTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAAt                                                                                                       >  3:106068/1‑149 (MQ=255)
                                             cTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAAtt                                                                                                      >  4:1319223/1‑149 (MQ=255)
                                               aCATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATtgt                                                                                                    <  2:1100477/149‑1 (MQ=255)
                                                 aTCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtgg                                                                                                  >  4:2171638/1‑149 (MQ=255)
                                                    gTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTaa                                                                                                        <  1:248370/140‑1 (MQ=255)
                                                         tGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcatc                                                                                          >  3:1569285/1‑149 (MQ=255)
                                                           cAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcatcat                                                                                        >  1:138744/1‑149 (MQ=255)
                                                            aaTTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATg                                                                                       <  2:829873/149‑1 (MQ=255)
                                                              tttATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGt                                                                                     <  4:617533/149‑1 (MQ=255)
                                                               ttATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTg                                                                                    <  4:1162828/149‑1 (MQ=255)
                                                                tATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGa                                                                                   <  3:1627067/149‑1 (MQ=255)
                                                                  ttGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtggtg                                                                                                >  4:1071545/1‑134 (MQ=255)
                                                                    gATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGt                                                                                                            >  4:1498786/1‑120 (MQ=255)
                                                                    gATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCtt                                                                               <  3:359027/149‑1 (MQ=255)
                                                                    gATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCtt                                                                               >  2:2031278/1‑149 (MQ=255)
                                                                     aTTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTg                                                                              >  3:1510787/1‑149 (MQ=255)
                                                                      ttttGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGc                                                                             >  2:2447790/1‑149 (MQ=255)
                                                                      ttttGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGc                                                                             >  3:2205087/1‑149 (MQ=255)
                                                                      ttttGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGc                                                                             <  3:1319223/149‑1 (MQ=255)
                                                                       tttGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCt                                                                            <  1:663680/149‑1 (MQ=255)
                                                                       tttGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCt                                                                            <  2:2381734/149‑1 (MQ=255)
                                                                           cAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGc                                                                             >  2:1736468/1‑144 (MQ=255)
                                                                           cAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGt                                                                        <  4:454263/149‑1 (MQ=255)
                                                                            aaGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTg                                                                       >  1:588983/1‑149 (MQ=255)
                                                                                 tttGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCtgt                                                                  >  4:2245/1‑149 (MQ=255)
                                                                                 tttGTGGGTCGGATAAGGCGTTCACGCCGCATCCAGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCtgt                                                                  <  1:1088981/149‑1 (MQ=255)
                                                                                  ttGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCtgtg                                                                 <  1:1695168/149‑1 (MQ=255)
                                                                                  ttGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCtgtg                                                                 <  2:2046523/149‑1 (MQ=255)
                                                                                      gggTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATg                                                                                       >  2:1042283/1‑123 (MQ=255)
                                                                                       ggTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgcagc                                                            <  2:736792/149‑1 (MQ=255)
                                                                                           ggATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCatta                                                        >  1:22179/1‑149 (MQ=255)
                                                                                            gATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGa                                                                                                                              >  2:2503376/1‑78 (MQ=255)
                                                                                             aTAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCattatt                                                      >  4:81510/1‑149 (MQ=255)
                                                                                                 ggCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTc                                                  >  4:725130/1‑149 (MQ=255)
                                                                                                 ggCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTc                                                  <  1:1942209/149‑1 (MQ=255)
                                                                                                   cGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGc                                                <  2:182709/149‑1 (MQ=255)
                                                                                                            cgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCt                                                                    <  1:2393948/120‑1 (MQ=255)
                                                                                                             gcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCgg                                      <  2:1345241/149‑1 (MQ=255)
                                                                                                              cATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAttttttt                                                                                                                                  <  1:1554092/56‑1 (MQ=255)
                                                                                                                    gCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggg                               <  3:801226/149‑1 (MQ=255)
                                                                                                                    gCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggg                               >  2:87949/1‑149 (MQ=255)
                                                                                                                    gCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggg                               <  2:1501761/149‑1 (MQ=255)
                                                                                                                      aaaaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggggc                             <  2:22179/149‑1 (MQ=255)
                                                                                                                       aaaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTCACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggggca                            <  1:787152/149‑1 (MQ=255)
                                                                                                                        aaaCAACGAGCCAATAAAAATACCGGCGTGATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCAt                           >  3:1401472/1‑149 (MQ=255)
                                                                                                                           caacGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGc                        <  3:465234/149‑1 (MQ=255)
                                                                                                                           caacGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGc                        <  1:943548/149‑1 (MQ=255)
                                                                                                                            aacGAGCCAATAAAAATTCCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCa                       >  3:1375288/1‑149 (MQ=255)
                                                                                                                            aacGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCa                       >  1:115040/1‑149 (MQ=255)
                                                                                                                             acGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCaa                      <  3:754077/149‑1 (MQ=255)
                                                                                                                                 gCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCaa                  >  1:2000326/1‑149 (MQ=255)
                                                                                                                                 gCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCaa                  >  3:2056708/1‑149 (MQ=255)
                                                                                                                                 gCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGCGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCaa                  >  1:1785407/1‑149 (MQ=255)
                                                                                                                                   gtaaaaaaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggg                               >  1:840835/5‑134 (MQ=39)
                                                                                                                                    aaTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAatat               >  3:2254410/1‑149 (MQ=255)
                                                                                                                                     aTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAAtatt              >  3:589127/1‑149 (MQ=255)
                                                                                                                                     aTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAAtatt              >  1:1246755/1‑149 (MQ=255)
                                                                                                                                     aTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAAtatt              >  3:2015276/1‑149 (MQ=255)
                                                                                                                                         aaaTACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATcc          >  2:1456524/1‑149 (MQ=255)
                                                                                                                                         aaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCtt                                                                               <  2:1673802/80‑1 (MQ=38)
                                                                                                                                         aaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggggc                             >  1:298813/1‑130 (MQ=255)
                                                                                                                                             aCCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCCCGgt      <  3:2423023/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCCCGGTGTGC  >  NC_000913/1113397‑1113690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: