Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,117,122 C→T R34H (CGC→CAC)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,117,1220CT96.6% 488.0 / NA 149R34H (CGC→CAC) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (67/77);  minor base G (1/4);  total (68/81)
Fisher's exact test for biased strand distribution p-value = 3.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

AATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAG  >  NC_000913/4116853‑4117391
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                             
aaTCGTCTAAACCGTAAGGTTTTAATGGCTCGAAGATTTCTGCGGTACGACGGCACGGCCAGACAATGGCTTTATAGATAGTCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCg                                                                                                                                                                                                                                                                         <  2:219398/276‑1 (MQ=255)
    ttttaagacGTCAAGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTCTAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGaca                                                                                                                                                                                                                                                                      <  4:532781/267‑1 (MQ=255)
    tttaaaaCCGTCACGTTTTAAATGTGCGCAGTTTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGGTAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGaca                                                                                                                                                                                                                                                                      <  4:347283/271‑1 (MQ=255)
     tctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCCGCCCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGaca                                                                                                                                                                                                                                                                      >  1:243777/1‑274 (MQ=255)
     tctAAACCGTCACGTTTTAAATGCTCGCAGATTTCGGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGCCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGaca                                                                                                                                                                                                                                                                      <  1:384615/274‑1 (MQ=255)
      ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATAGCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGCTTTCCATTGGTTCGTGTTCTACCAACCCTGCTTTTGGGTAGTTTTGCCTAATTTCGTCCTGCCACCCg                                                                                                                                                                                                                                                                    >  4:401276/1‑275 (MQ=255)
      ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCt                                                                                                                                                                                                                                                                  >  4:384166/1‑276 (MQ=255)
         aaCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTTCTCGAATTCGTGCTGTGaccct                                                                                                                                                                                                                                                                    >  4:666341/1‑269 (MQ=255)
         aaCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTa                                                                                                                                                                                                                                                                 <  2:276449/275‑1 (MQ=255)
          aCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAAt                                                                                                                                                                                                                                                               <  4:593366/276‑1 (MQ=255)
            ctgcACGTTTTAAATGCTCCCAGATTTCTGCGGTACGACGGCACTTCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTa                                                                                                                                                                                                                                                                 <  2:666341/269‑1 (MQ=255)
              tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTTCCAGCGTGGAGCTTTGGGTGGCCCAGTTTTCCATTGGGTCGTTTTCTAACCAACTTGGTTTTGGGTAGATTTTCTCAACTTCGTGCTTCGACACGCTAATGa                                                                                                                                                                                                                                                             >  3:513880/1‑274 (MQ=255)
              tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACTAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAACTGTTTTTGGGTGGATTTGCTAAAATTCGTTTTGCGACACGCAAATgtt                                                                                                                                                                                                                                                            >  4:703409/1‑273 (MQ=255)
              tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGtgctgc                                                                                                                                                                                                                                                                          >  4:552232/1‑261 (MQ=255)
              tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGata                                                                                                                                                                                                                                                           <  4:281958/276‑1 (MQ=255)
                aCGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCTGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGTTTTGCTCAAATTCGTTCTGCGACACGCTAATGata                                                                                                                                                                                                                                                           >  2:180870/1‑274 (MQ=255)
                 cGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGac                                                                                                                                                                                                                                                                       <  2:552232/261‑1 (MQ=255)
                    attAAATGGTCGAAGATTTCTGCGGTACGGCGCCCCTGCCATGCAATGGCGTTTTAGATTGGCTATCCGGTTTCTTTTTCCCAGACATTAGGGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCa                                                                                                                                                                                                                                                     <  2:422271/275‑1 (MQ=255)
                         aTGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                                                                                               <  2:384166/276‑1 (MQ=255)
                              cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCACCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCTGGTTCTACCCAACTGGGTTTTTGGTTGGTTTGCTCCAATTCGTGCTGCGAAACGCTAATGCTATGTGCATCGTGa                                                                                                                                                                                                                                               >  1:409358/1‑272 (MQ=255)
                                     ttCTGGGATACGACGGCACTGCCAGGCAATGTCGTTATAGATAGGCTTGCCGGTTGCTTTTTCCCGGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGa                                                                                                                                                                                                                                     <  1:61360/275‑1 (MQ=255)
                                           ggTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgc                                                                                                                                                                                                                               <  1:503055/275‑1 (MQ=255)
                                                gacgGCAATGCCAGACAATGTGGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGAAAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAg                                                                                                                                                                                                                          <  3:585743/275‑1 (MQ=255)
                                                       cTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGTACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTTGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATTTTGGCATCGTGATCCATTTCGACCCCCCGGGAGCtggttg                                                                                                                                                                                                                   >  1:48952/1‑273 (MQ=255)
                                                       cTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTGTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                                                                                                                                                                   <  1:135387/275‑1 (MQ=255)
                                                            aGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGccc                                                                                                                                                                                                              <  3:536017/275‑1 (MQ=255)
                                                                  tGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTTGATTTGCTTAAATTCGGGTTGCGACACGCTAATGATATTGGCATCGTGTTCCTTTACGACCGCGCGGGAGCTGTTGGTGCCCttgccgt                                                                                                                                                                                                       >  2:416295/1‑270 (MQ=255)
                                                                        tataGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCa                                                                                                                                                                                                  <  2:564607/275‑1 (MQ=255)
                                                                         atagatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCa                                                                                                                                                                                                  <  1:533929/274‑1 (MQ=255)
                                                                         atagatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCa                                                                                                                                                                                                  <  3:507397/274‑1 (MQ=255)
                                                                          tagatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACCCTAATGTTTTTGGCATGGTGATCAATAACGACCGCGGGGGAGCTGGTGGTTCCCTGGCGGAGGGCAAc                                                                                                                                                                                                >  4:594084/1‑275 (MQ=255)
                                                                               agGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGGTTTGCTCAATTGCGTGCTGCGCCACGCTCATGATATTGGCTTGTTGTTCCATTACGAACGCCCGGGCGCTGTTGGGGCCTTGTCCTCGCGCAATGatac                                                                                                                                                                                           >  4:634752/1‑274 (MQ=255)
                                                                                 gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGTTCGTTTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTACTGATATTGGCATCGTGATCCATTACGACCGCGCGGTAGCTGGTGGTTCCCTGGTCGAGCGCCACGCTATAtt                                                                                                                                                                                        >  4:731008/1‑276 (MQ=255)
                                                                                   ttGCCGGTTTCTTTTTCCCAGACAATGGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGAAATTTGATCGGAACGGATATCGGCTTTCGCCCGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAttt                                                                                                                                                                                       <  2:738245/275‑1 (MQ=255)
                                                                                    tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGCTTTGCTCAAATTCGTGCTGCGACACGCTACTGATATTGGCATCGTGATCCTTTACGACCGCCCGGGAGCTGGTGGTGCCTTGGGTGAGCGCAACGGTTAtttttt                                                                                                                                                                                    >  4:559462/1‑276 (MQ=255)
                                                                                    tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGATGCGACACGCTCATGCTATTGGCATCGTGCTCCATTACGACTGGGCGGGAGCTGGTGGTGCCCTGGTCGAGCGACACGGTATAtttt                                                                                                                                                                                      >  3:651965/1‑275 (MQ=255)
                                                                                    tGCCGGTTTCTTTTTCCCAGACAATAGTGGTATCACGCTGGTTGGTAATACCGATAGCTGCAATTAGATCGGAACTGATATCGGCTTTAGCCAGCACTTCTACCAGCGTGGAGGTTTGGGTGGCACAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACTAGCTAATGAGATTGGCATCGTGATCCATTACGCCCGCGCGGGGGCTGGTGTTGCACTGTTCGTGAGCACCGATATAttttt                                                                                                                                                                                     >  4:619408/1‑276 (MQ=255)
                                                                                           ttCTTTTTCCCAGTCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGt                                                                                                                                                                              <  2:358242/276‑1 (MQ=255)
                                                                                           ttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATCTATTTTTTTTTAg                                                                                                                                                                               >  2:604958/1‑275 (MQ=255)
                                                                                           ttCTTGTTCCCAGCCAATGGTGGTTTCACGCTGGTTCGTAATGCCGATACCTGCAAGTTGATCGGAACTGGTATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATGTCCATTGGGTAGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAg                                                                                                                                                                               <  2:619408/275‑1 (MQ=255)
                                                                                            tttttttCCCCGACAATTGTGGTTTACCCATTGGTCGGAAAACCGAGAGCTTCAATTTGTTCGGTACTGCTATCTGCTTTCGCCAGCACTTCTACCAGCGTGGAGAGTTGGGGGGCCCAGATTTCCATTGGGTCGGGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAGTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGt                                                                                                                                                                              <  3:177268/273‑1 (MQ=255)
                                                                                                       gACAATAGTGGTTTCACTCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAGCTGAGATCGGCTTTCGCCAGCACTTCTACCATCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGGGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAAtt                                                                                                                                                                   <  2:496857/275‑1 (MQ=255)
                                                                                                            tAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCg                                                                                                                                                             <  2:83893/276‑1 (MQ=255)
                                                                                                            tAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGGGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTGAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGGTCGGGTGTCTCCCTGGTCTAGGGCAACGATATCTTTTTTTTTAGTCTTTTTTCAtttaccag                                                                                                                                                             >  4:4183/1‑270 (MQ=255)
                                                                                                             aGTGGTTTCACGCTGGGTCGTAATACCGAGAGCTGCAATTTGAGCGGAACTGATATCGGCCTTCGCCAGCACTTCTGCCGGCGTGGGGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCg                                                                                                                                                             <  3:48951/275‑1 (MQ=255)
                                                                                                                ggTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                                                                                                                                          <  1:516895/275‑1 (MQ=255)
                                                                                                                ggTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGAGATTGGCATCGTGGTCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                                                                                                                                          <  1:394471/275‑1 (MQ=255)
                                                                                                                    tCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATc                                                                                                                                                                                                                                                   >  1:253994/1‑182 (MQ=255)
                                                                                                                    tCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCACCGATGTATTTTTTTTCAGTCATGTTTAATTGTTCCGTTGTCatg                                                                                                                                                     >  4:555974/1‑275 (MQ=255)
                                                                                                                        gCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                                                                                               <  3:253993/182‑1 (MQ=255)
                                                                                                                               cGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttttttt                                                                                                                                                                                  >  2:95210/1‑236 (MQ=255)
                                                                                                                                           aGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTg                                                                                                                                                                                                                                                        >  4:718421/1‑154 (MQ=255)
                                                                                                                                             atGCAATTTGATCGCGACTGTTATCGGCTTTTGCCTGCACTTCTACCGTCTTGGGGCTTTGGGTGGCCCAGATTTCCATTGGGTCGGGTTCTCCCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGa                                                                                                                             <  2:325141/274‑1 (MQ=255)
                                                                                                                                              ttcAATTTGATCTGAGCTTATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGGGCTTTGGGGGGTCCTGATTTCCGTTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGTTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAg                                                                                                                           <  2:703409/274‑1 (MQ=255)
                                                                                                                                                cAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCTCAGATTTCCATTGGTTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACCCGCTAATGATATTGGCATCGTGATCCATTACTTCCGTGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAGTTGTCCCGTTGTCATATTCCAGCGAAGCTTTTTTTTCTGAAgg                                                                                                                          >  1:593711/1‑275 (MQ=255)
                                                                                                                                                            aaCTGATTTCGGCTTTCGCCAGCACTTCTACCAGCGTCGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTcc                                                                                                             <  2:592321/276‑1 (MQ=255)
                                                                                                                                                            aaCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTcc                                                                                                             <  2:594084/276‑1 (MQ=255)
                                                                                                                                                                 atatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATTTTTAATTGTCCCGTATTCATTTTACAGCGAAGCTTTTTGTTCTGAAGGAGTTCTGtttcttctt                                                                                                          >  1:471466/1‑268 (MQ=255)
                                                                                                                                                                  tatCGGCTTTCGCCTGCACTTCTACCGGCTGGGAGCTTGTGGTGGCCCAGATTTCCATTAGGTCGTGTTCTACCCAATCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCAGTACGACCGCGCGGGAGCTAGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTttc                                                                                                        <  1:672542/275‑1 (MQ=255)
                                                                                                                                                                     cGGGTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGtt                                                                                                                                 <  3:471465/247‑1 (MQ=255)
                                                                                                                                                                      ggCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGGGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGTGAAGCTTTTTCTTCTGAAGGAGTTGTGGTTTCCTTttcttc                                                                                                     >  1:154275/1‑274 (MQ=255)
                                                                                                                                                                      ggCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTc                                                                                                                                                                                 >  1:308744/1‑198 (MQ=255)
                                                                                                                                                                      ggCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCATTCATGTTTAATTGTCCCGTCGTCATTTTTCTGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCGTCca                                                                                                   >  2:112470/1‑276 (MQ=255)
                                                                                                                                                                      ggCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACCGCGCAGCTTTTTGTTCTGACGGAGTTGTGGTTTCCTTTTCTTcc                                                                                                    >  2:445515/1‑275 (MQ=255)
                                                                                                                                                                       gCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCAGCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCcac                                                                                                  <  2:204189/276‑1 (MQ=255)
                                                                                                                                                                          ttCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                                                                                               <  1:534937/132‑1 (MQ=255)
                                                                                                                                                                           tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCCTGTTTAATTGTCCCGTAGTCATATTAAAGCGATGCTGTTTTTTTTTATGTAGTTGGTGTTTCCTTttgttgcgacaca                                                                                              >  4:297078/1‑268 (MQ=255)
                                                                                                                                                                           tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTccc                                                                                                                                                              >  3:191342/1‑212 (MQ=255)
                                                                                                                                                                                  cACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGCTTTGCTCAAATTCGTGCTGCTACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGACCTGGTGGTTCCCTGGTCTAGCTCCACGATATATTTTTTTTGAGTCCTGTTTCATTGTCCCGTAGTCCTTTTAAACGTACGCTTTTTGTTTTGACAGAGTTTTGGTTTCCTTTTCCTCCAAATCatatatat                                                                                       >  4:540826/1‑276 (MQ=255)
                                                                                                                                                                                       cTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgc                                                                                                                                                                                                                                 >  4:302877/1‑133 (MQ=255)
                                                                                                                                                                                          ccAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgcg                                                                                                                                                                                                                              <  2:302877/133‑1 (MQ=255)
                                                                                                                                                                                               gTGGAGCTTTGGGTGGCCCAGATTTCCCTTGGGTCGTGTTCTACCCCACCTGGTTTTGGGTAGATTTGCCCAAATTCGTGCTGTGACACGTTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCGTGGTCGAGCGCAACGATATATTTTTTTTAAGTCATGTTTAATTTTCCCGTAGTCATATTCAATCGAAGCTTTTTGTTCTGATGGATTTGTGGTTTCTTTTCCTTCCCCAACACAGata                                                                                        >  3:396943/1‑262 (MQ=255)
                                                                                                                                                                                                        tGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCGTGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAAt                                                                 <  2:297676/276‑1 (MQ=255)
                                                                                                                                                                                                        tGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATgg                                                                         >  1:413816/1‑268 (MQ=255)
                                                                                                                                                                                                        tGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAAt                                                                 <  2:520539/276‑1 (MQ=255)
                                                                                                                                                                                                         ggttGGCCCGTATGTCCGTTGGGTCGTGTTCTCCCCAACCTGGTTTTGGGTAGATTTGACAAAATGCGTGCGCCGACACGCTAATGATATTGGCATCGTGATCCAGTACGAGCGCGCGGGGGCTGGTGGTGCCCTGGTCGCGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAAt                                                                 <  3:139380/272‑1 (MQ=255)
                                                                                                                                                                                                           gTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                                                                                            <  2:641884/182‑1 (MQ=255)
                                                                                                                                                                                                                      ttCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTcc                                                                                                                                                               >  4:354047/1‑168 (MQ=255)
                                                                                                                                                                                                                      ttCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATGGTcc                                                                                                                                                               >  4:220732/1‑168 (MQ=255)
                                                                                                                                                                                                                         cATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                                                                                            <  2:220732/168‑1 (MQ=255)
                                                                                                                                                                                                                         cATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCa                                                 >  2:709810/1‑275 (MQ=255)
                                                                                                                                                                                                                            tGGGTAGTGTGCTACCCGACCTGGTTTTGGGTAGATGTGCTCAAATTCGTGCTGCAACAAGCGAATGATATTGGAATCGTGTTCCAGTACGACCGCTCGGGTGCTGGTGGTGCACTGGTCGAGAGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTCGTCATATTACAGCGAAGCTTGTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAAt                                              <  4:189791/275‑1 (MQ=255)
                                                                                                                                                                                                                              ggTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATCTATTTTTTTTCAGTCATGTTTAATTGCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGACGGAGTTGTGGTTTCCTTTTCTTCCACAACACAATTATCCCAAGGCAAATGGCTACCAATCAGTTTCCGGTAGGCAAATGCa                                           >  4:149175/1‑275 (MQ=255)
                                                                                                                                                                                                                              ggTCGGGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGTGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGc                                            <  3:543918/275‑1 (MQ=255)
                                                                                                                                                                                                                                        tACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTcc                                                                                                                                                               >  2:445643/1‑150 (MQ=255)
                                                                                                                                                                                                                                          cccAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCAACTACAACcg                                 >  3:437449/1‑274 (MQ=255)
                                                                                                                                                                                                                                           ccAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                                                                                            <  4:445643/150‑1 (MQ=255)
                                                                                                                                                                                                                                              aCCTTGTTTGGGGTATATTTGCTCAAATTCGGGTGGCGACACGCTAGAGATTTTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGTTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACATCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGcc                             <  3:409357/274‑1 (MQ=255)
                                                                                                                                                                                                                                              aCCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGc                                                                                    <  1:565838/218‑1 (MQ=255)
                                                                                                                                                                                                                                               ccTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTa                                                                                                                                                  >  3:577022/1‑156 (MQ=255)
                                                                                                                                                                                                                                                  ggggtttggTAGATTTGCTCCAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGa                        <  3:681821/268‑1 (MQ=255)
                                                                                                                                                                                                                                                  ggTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAg                                                                                                                                          >  1:287718/1‑161 (MQ=255)
                                                                                                                                                                                                                                                   gTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGc                                                                                                                                              <  1:577023/156‑1 (MQ=255)
                                                                                                                                                                                                                                                    ttttGGGTAGGTTTGCTCAAATTGGGGCTGCGACACGCTAATGTTATTGGCATCGGGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATa                      <  3:428730/275‑1 (MQ=255)
                                                                                                                                                                                                                                                      ttGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCttt                                                                                                                                      <  3:287717/161‑1 (MQ=255)
                                                                                                                                                                                                                                                         ggTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGGTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTGCCTTTTCGTCCACAAGCCAGATATAGAAAGGCAAAGGGCGAGCAATAAGTGTGCGGTTGGCAAATGCGACTACAACCTCACCAACAAAAgggaca                >  4:177131/1‑273 (MQ=255)
                                                                                                                                                                                                                                                            aGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAc                                                                                                                                                                                                                                    <  4:274987/61‑1 (MQ=255)
                                                                                                                                                                                                                                                              aTTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGCGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGACGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCCCAAGGCAAATGGCGACGCCTTAGCTTCTGGTATGCAAATGCACCTGCAATCCCCCCCATGACAgggacccaaa            >  4:249990/1‑268 (MQ=255)
                                                                                                                                                                                                                                                                 tGCTCAAATTCGTGCGGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCg         <  2:554729/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                    tCAAATTGGTGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCATGGTCGAGCGCAAAGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCAc     <  2:559462/276‑1 (MQ=255)
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                             
AATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAG  >  NC_000913/4116853‑4117391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: