| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,196,120 | G→A | 100% | intergenic (‑21/+212) | thiC ← / ← rsd | phosphomethylpyrimidine synthase/stationary phase protein, binds sigma 70 RNA polymerase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,196,120 | 0 | G | A | 96.4% | 284.3 / 0.3 | 84 | intergenic (‑21/+212) | thiC/rsd | phosphomethylpyrimidine synthase/stationary phase protein, binds sigma 70 RNA polymerase subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/3); new base A (49/32); total (49/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.87e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
GCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTAT > NC_000913/4196023‑4196211 | gCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGATAAATCAAAAAAGTTAAAGa < 2:58778/100‑1 (MQ=255) cGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGTGCGTTGTTCGCGGCGGGGCACTTTTTTGGCAGACATAGCGCATTCCAAAAAGTTAAAGa > 2:1454826/1‑99 (MQ=255) gCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTgg > 1:308750/1‑100 (MQ=255) gCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTgg > 1:308761/1‑100 (MQ=255) cTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCt > 1:1798612/1‑100 (MQ=255) cTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCt > 1:1444823/1‑100 (MQ=255) cTTCCAGGGTGTCGATAAAACGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCACAAAGTTAAAGACGTGGCt > 2:637939/1‑100 (MQ=255) tCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTg < 1:1326128/100‑1 (MQ=255) tCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTg > 1:818525/1‑100 (MQ=255) tCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTg < 2:556479/100‑1 (MQ=255) tCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTg > 2:464403/1‑100 (MQ=255) gggTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAg < 1:837111/100‑1 (MQ=255) tgtTGATTAAATGTTGGGCCCGGGCAAGTGATTCGCGTCGGGACAGTTTTGTTGCAGACATAGCTAAGTCCTAAATGTTAAGGTCGTGGCTTGTCAgacg < 2:1862542/100‑1 (MQ=255) tCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAgacgac > 2:2004704/1‑100 (MQ=255) cGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGacgacg < 1:1002733/100‑1 (MQ=255) gATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACgg > 2:773671/1‑100 (MQ=255) tAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGAt < 2:1106199/100‑1 (MQ=255) aaaaTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATg > 2:1503586/1‑100 (MQ=255) aaaTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGa < 2:1705651/100‑1 (MQ=255) aaaTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGa < 1:1180407/100‑1 (MQ=255) tGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGc < 1:1762733/100‑1 (MQ=255) ttGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCaa < 2:818522/100‑1 (MQ=255) tctgCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAag < 2:408064/97‑1 (MQ=255) tGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAag > 2:796640/1‑100 (MQ=255) gggCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAaga > 2:405959/1‑100 (MQ=255) ggCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAagag < 1:553380/100‑1 (MQ=255) gCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAgaga > 2:1193121/1‑100 (MQ=255) gCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAgaga > 1:1550579/1‑100 (MQ=255) gCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAgaga < 2:719205/100‑1 (MQ=255) gCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAgaga > 1:408067/1‑100 (MQ=255) cGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGa > 2:998544/1‑100 (MQ=255) cgcgTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATcgc < 2:1435172/100‑1 (MQ=255) cgTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATcgccg < 1:1828775/100‑1 (MQ=255) cgTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATcgccg > 1:240968/1‑100 (MQ=255) cgTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATcgccg > 1:991077/1‑100 (MQ=255) ttgttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCa > 2:618131/1‑100 (MQ=255) gttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAgg > 2:550261/1‑100 (MQ=255) ttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAggg < 1:1045779/100‑1 (MQ=255) ttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAggg < 1:1644405/100‑1 (MQ=255) tCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAgggg < 1:474284/100‑1 (MQ=255) cgcgGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgc < 1:1740159/100‑1 (MQ=255) cgcgGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgc < 2:1444813/100‑1 (MQ=255) cgccgcGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgc < 1:187872/100‑1 (MQ=255) cggGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgatgc > 2:21052/1‑100 (MQ=255) cggGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgat > 2:965099/1‑98 (MQ=255) ggTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgatgcga < 1:173059/100‑1 (MQ=255) gTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgctgc > 2:1599608/1‑97 (MQ=255) aGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGc > 1:637252/1‑100 (MQ=255) gTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCa > 1:948564/1‑100 (MQ=255) gTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCa < 1:1717757/100‑1 (MQ=255) gTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCa > 1:678910/1‑100 (MQ=255) ttttGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAg < 1:1480100/100‑1 (MQ=255) tgttgCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGAtt > 2:251789/1‑100 (MQ=255) tgttgCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGAtt > 1:1633208/1‑100 (MQ=255) tgttgCAGACATAGCTCATTACAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGAtt < 1:1530305/100‑1 (MQ=255) gttgCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTa < 1:672838/100‑1 (MQ=255) gttgCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTa < 1:280445/100‑1 (MQ=255) tgCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAAc > 1:1217492/1‑100 (MQ=255) gCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAAct > 1:311216/1‑100 (MQ=255) gCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAAct < 1:901150/100‑1 (MQ=255) gCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGAAGAAGCAAGAGACGATCGCCGCAGGGGCGAAGCGATAGCAGATTAAc > 2:1453099/1‑99 (MQ=255) cAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAActc > 2:887537/1‑100 (MQ=255) cAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAActc < 1:745047/100‑1 (MQ=255) aGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAActct > 1:328319/1‑100 (MQ=255) aGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAActct < 2:540281/100‑1 (MQ=255) gACATAGCTCAGTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCtt < 1:744010/100‑1 (MQ=255) tAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTc > 2:1961486/1‑100 (MQ=255) aGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTcc > 2:469218/1‑100 (MQ=255) gCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTccc > 2:619462/1‑100 (MQ=255) cTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCt > 2:1036853/1‑100 (MQ=255) tCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCtt > 2:1547662/1‑100 (MQ=255) tCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCtt > 2:1982396/1‑100 (MQ=255) tCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCtt > 1:1836911/1‑100 (MQ=255) cATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTc > 1:700440/1‑100 (MQ=255) ttCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGc < 2:1511256/100‑1 (MQ=255) tCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCa > 1:1250978/1‑100 (MQ=255) tCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCa > 1:1238313/1‑100 (MQ=255) ccAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAg > 1:655840/1‑100 (MQ=255) ccAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAg > 2:844277/1‑100 (MQ=255) cAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAgg < 1:61638/100‑1 (MQ=255) cAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAgg > 2:744652/1‑100 (MQ=255) cAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAgg > 1:2062210/1‑100 (MQ=255) aaaGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTAt > 1:752650/1‑100 (MQ=255) aaaGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTAt < 1:229159/100‑1 (MQ=255) | GCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTAT > NC_000913/4196023‑4196211 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |