Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,196,120	G→A	intergenic (‑21/+212)	thiC ← / ← rsd	phosphomethylpyrimidine synthase/stationary phase protein, binds sigma 70 RNA polymerase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,196,120	0	G	A	100.0%	327.4 / NA	92	intergenic (‑21/+212)	thiC/rsd	phosphomethylpyrimidine synthase/stationary phase protein, binds sigma 70 RNA polymerase subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/50);  total (42/50)

CCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAG  >  NC_000913/4196029‑4196214                                                                                            |                                                                                               ccTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGc                                                                                        >  1:1865929/1‑100 (MQ=255)   ttCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCtt                                                                                      >  1:68557/1‑100 (MQ=255)     ccAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGt                                                                                    >  2:1049999/1‑100 (MQ=255)      gatcttgtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTc                                                                                   <  2:1591435/95‑1 (MQ=255)      cAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTc                                                                                   <  1:1216816/100‑1 (MQ=255)      cAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTc                                                                                   >  1:1761970/1‑100 (MQ=255)       aGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAATTAAAGAAGTGGCTTGTCa                                                                                  >  1:1291890/1‑100 (MQ=255)        gggTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAg                                                                                 <  2:1707878/100‑1 (MQ=255)        gggTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAg                                                                                 <  1:1948217/100‑1 (MQ=255)        gggTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAg                                                                                 <  2:2406150/100‑1 (MQ=255)         tgtgtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCACGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAga                                                                                >  1:1990216/2‑100 (MQ=255)         ggTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAga                                                                                <  1:2580613/100‑1 (MQ=255)         ggTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAga                                                                                <  2:597267/100‑1 (MQ=255)          gtgtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAgac                                                                               <  2:1921165/100‑1 (MQ=255)          gtgtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAgac                                                                               <  1:2755/100‑1 (MQ=255)           tgtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAgatc                                                                              >  1:1591443/1‑98 (MQ=255)           tgtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAgacg                                                                              <  2:1324816/100‑1 (MQ=255)            gtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAgacga                                                                             >  1:1587562/1‑100 (MQ=255)            gtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAgacga                                                                             >  1:356200/1‑100 (MQ=255)               gATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACgg                                                                          >  2:1496757/1‑100 (MQ=255)                aTAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGa                                                                         <  2:569033/100‑1 (MQ=255)                aTAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGa                                                                         <  1:1341385/100‑1 (MQ=255)                 tAAAATGTTGGGCCCCGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTTGCTTGTCAGACGACgg                                                                          <  2:705213/98‑1 (MQ=255)                  aaaaTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATg                                                                       >  2:2152596/1‑100 (MQ=255)                   aaaTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATg                                                                       <  2:830299/99‑1 (MQ=255)                   aaaTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGa                                                                      >  1:1407173/1‑100 (MQ=255)                   aaaTGTTGGGCCCGGGAGCGATGTTAGCGGCGGAACAGTTTTGTTGCAAACATAGCACATTCCAAAAAGTTAAAGACGTGGCTTTTGAGAAGACGGAtaa                                                                      >  2:1392421/1‑98 (MQ=255)                    aaTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGaa                                                                     <  2:1528144/100‑1 (MQ=255)                    aaTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGaa                                                                     >  1:1115990/1‑100 (MQ=255)                     aTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAg                                                                    <  2:2232494/100‑1 (MQ=255)                     aTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAg                                                                    <  2:1556947/100‑1 (MQ=255)                       gTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCa                                                                  <  1:1315974/100‑1 (MQ=255)                          gggCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCaa                                                                 >  1:2742770/1‑98 (MQ=255)                          gggCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAaga                                                               >  2:324603/1‑100 (MQ=255)                          gggCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAaga                                                               <  2:2287039/100‑1 (MQ=255)                          gggCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAaga                                                               >  1:2119769/1‑100 (MQ=255)                           ggCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAagag                                                              >  1:2619466/1‑100 (MQ=255)                           ggCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAaga                                                               >  1:2129624/1‑99 (MQ=255)                                gggCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGa                                                          >  1:1397727/1‑99 (MQ=255)                                 ggCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAag                                                                <  2:2633669/92‑1 (MQ=255)                                  gcgcGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGATGATcg                                                       <  2:506882/100‑1 (MQ=255)                                  gcgcGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATcg                                                       <  1:1594503/100‑1 (MQ=255)                                       ttgttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCa                                                  >  1:498903/1‑100 (MQ=255)                                         gttCGCGGTGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAgg                                                <  1:999630/100‑1 (MQ=255)                                         gttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCa                                                  >  1:2498706/1‑98 (MQ=255)                                         gttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAgg                                                >  2:648821/1‑100 (MQ=255)                                         gttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAgg                                                <  1:98976/100‑1 (MQ=255)                                           tCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAgggg                                              <  2:1939013/100‑1 (MQ=255)                                            cgcgGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgc                                             >  2:936403/1‑100 (MQ=255)                                            cgcgGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgc                                             <  1:1806369/100‑1 (MQ=255)                                              cggcggGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcga                                           >  2:554776/1‑100 (MQ=255)                                               ggcggGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgat                                          <  2:1761961/100‑1 (MQ=255)                                                   ggTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgatgcga                                      >  2:2116063/1‑100 (MQ=255)                                                   ggTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgatgcg                                       <  2:1835659/99‑1 (MQ=255)                                                    gTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgatgcgat                                     <  2:1995773/100‑1 (MQ=255)                                                     tCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATa                                    <  2:2516324/100‑1 (MQ=255)                                                      cAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAg                                   >  1:1458784/1‑100 (MQ=255)                                                        gTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCa                                 <  1:2654971/100‑1 (MQ=255)                                                          tttGTTGCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGa                               <  1:2551422/100‑1 (MQ=255)                                                           ttgttgCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGAt                              <  2:2619451/100‑1 (MQ=255)                                                           ttgttgCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGAt                              <  1:554780/100‑1 (MQ=255)                                                             gttgCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTa                            <  1:291881/100‑1 (MQ=255)                                                             gttgCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTa                            <  2:1865920/100‑1 (MQ=255)                                                             gttgCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTa                            <  1:544555/100‑1 (MQ=255)                                                               tgCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAAc                          <  2:498900/100‑1 (MQ=255)                                                               tgCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAAc                          >  2:274596/1‑100 (MQ=255)                                                                gCAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAAct                         >  1:853548/1‑100 (MQ=255)                                                                 cAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAActc                        >  2:1414441/1‑100 (MQ=255)                                                                 cAGACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAActc                        <  2:1291884/100‑1 (MQ=255)                                                                   gACATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCtt                      <  2:2168104/100‑1 (MQ=255)                                                                     cATAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGt                    <  1:1437997/100‑1 (MQ=255)                                                                       tAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTc                  >  1:1343807/1‑100 (MQ=255)                                                                       tAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTc                  <  2:68556/100‑1 (MQ=255)                                                                       tAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTc                  <  2:1070313/100‑1 (MQ=255)                                                                       tAGCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTc                  >  2:333898/1‑100 (MQ=255)                                                                         gCTCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCTCAGGGGCGATGCGATAGCAGATTAACTCTTGTTcc                 >  1:677222/1‑99 (MQ=255)                                                                           tCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCtt              <  2:1587554/100‑1 (MQ=255)                                                                           tCATTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCtt              <  2:356197/100‑1 (MQ=255)                                                                             aTTCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCtt              <  2:2032861/98‑1 (MQ=255)                                                                              ttCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGc           <  1:2152606/100‑1 (MQ=255)                                                                               tCCAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCa          <  1:1050005/100‑1 (MQ=255)                                                                                ccAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAg         >  1:159265/1‑100 (MQ=255)                                                                                ccAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAg         >  1:1463290/1‑100 (MQ=255)                                                                                ccAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAg         <  2:2119759/100‑1 (MQ=255)                                                                                ccAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAAATCTTAttaca                >  1:1935874/1‑90 (MQ=255)                                                                                 cAAAAAGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCAATAGCAGATTAACTCTTGTTCACTTCGCAgg        >  1:300102/1‑100 (MQ=255)                                                                                  aaaaaGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAgct       <  1:1913911/100‑3 (MQ=255)                                                                                  aaaaaGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGt       >  1:2569345/1‑100 (MQ=255)                                                                                  aaaaaGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGt       >  1:325189/1‑100 (MQ=255)                                                                                  aaaaaGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGt       >  2:583179/1‑100 (MQ=255)                                                                                   aaaaGTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTa      >  1:1703696/1‑100 (MQ=255)                                                                                       gTTAAAGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAg  >  1:167437/1‑100 (MQ=255)                                                                                            |                                                                                               CCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAG  >  NC_000913/4196029‑4196214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑