BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,400,396	G→A	E37K (GAG→AAG)	hfq →	global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,400,396	0	G	A	100.0%	501.8 / NA	139	E37K (GAG→AAG)	hfq	global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (57/82); total (57/82)

Only 100 of 139 total aligned reads displayed.

CAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTT  >  NC_000913/4400300‑4400491

cAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGt                                                                                              <  2:2348196/100‑1 (MQ=255)

cAATCTTTACAAGCTCCGTTCCTGAAAGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAg                                                                                               <  1:699784/99‑1 (MQ=255)

 aaTCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTc                                                                                             >  1:1682633/1‑100 (MQ=255)

 aaTCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTc                                                                                             <  2:1562057/100‑1 (MQ=255)

  aTCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCt                                                                                            <  1:1502449/100‑1 (MQ=255)

    cTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCttt                                                                                          <  2:2668024/100‑1 (MQ=255)

     tttACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCtttt                                                                                         >  1:2751371/1‑100 (MQ=255)

     tttACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCtttt                                                                                         <  1:1612162/100‑1 (MQ=255)

     tttACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCtttt                                                                                         <  2:421926/100‑1 (MQ=255)

      ttACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTg                                                                                        <  2:1669206/100‑1 (MQ=255)

      ttACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTg                                                                                        >  2:330843/1‑100 (MQ=255)

      ttACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTg                                                                                        <  2:470029/100‑1 (MQ=255)

      ttACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTg                                                                                        <  2:285106/100‑1 (MQ=255)

      ttACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTg                                                                                        <  1:861502/100‑1 (MQ=255)

       tACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGa                                                                                       <  2:1111212/100‑1 (MQ=255)

         cAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATc                                                                                     >  1:490620/1‑100 (MQ=255)

             ctCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGtt                                                                                 >  1:2361703/2‑100 (MQ=255)

                 gTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTg                                                                             <  2:2208693/100‑1 (MQ=255)

                    ccTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATc                                                                          >  2:311262/1‑100 (MQ=255)

                    ccTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATc                                                                          >  1:510267/1‑100 (MQ=255)

                     cTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATcc                                                                         >  1:2375779/1‑100 (MQ=255)

                       gAACGCATTGCGTCGGGAACGTGTTGCAGTTTCTATTTATGTGGTGAATGGTATTAAGCTGCAAGGGCACATCAAGTCTTTTGATCAGTTCGTGAt                                                                           <  2:143038/96‑1 (MQ=255)

                       gAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTg                                                                       <  2:2375766/100‑1 (MQ=255)

                         aCGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGAAAATCAAGTCTTTTGATCAGTTCGTGATCCTGtt                                                                     >  2:2429920/1‑100 (MQ=255)

                          cGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTg                                                                    >  2:1057646/1‑100 (MQ=255)

                           gCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGa                                                                   <  1:1127654/100‑1 (MQ=255)

                              tccgatctggAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGaaaa                                                                <  2:1540276/92‑1 (MQ=255)

                              cTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGaaaa                                                                >  1:868334/1‑100 (MQ=255)

                              cTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGaaaa                                                                <  1:96086/100‑1 (MQ=255)

                               tGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGaaaaa                                                               <  1:2199963/100‑1 (MQ=255)

                                gCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAac                                                              <  1:763276/100‑1 (MQ=255)

                                 cGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAaca                                                             <  1:636578/100‑1 (MQ=255)

                                  gTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAacac                                                            <  1:2257667/100‑1 (MQ=255)

                                    cGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACgg                                                          <  1:1007566/100‑1 (MQ=255)

                                     gggAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGt                                                         >  1:2681233/1‑100 (MQ=255)

                                     gggAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGt                                                         <  1:2295820/100‑1 (MQ=255)

                                      ggAACGTGTTCCATTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTc                                                        >  1:1914707/1‑100 (MQ=255)

                                      ggAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGaaaaagatcgga                                                        >  1:1540284/1‑93 (MQ=255)

                                      ggAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGt                                                         >  2:2310366/1‑99 (MQ=255)

                                        aaCGTGTTCTAGTTTCTTTTTATTTGGTGATTGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAg                                                      <  1:1614832/100‑1 (MQ=255)

                                          cGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGcc                                                    <  2:1920287/100‑1 (MQ=255)

                                           gtgtTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCa                                                   <  2:460828/100‑1 (MQ=255)

                                            tgtTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAg                                                  <  1:1878852/100‑1 (MQ=255)

                                              ttCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGAt                                                <  2:2776046/100‑1 (MQ=255)

                                               tCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATg                                               >  1:2096/1‑100 (MQ=255)

                                                ccAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATgg                                              <  1:1876869/100‑1 (MQ=255)

                                                ccAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATgg                                              >  2:1226648/1‑100 (MQ=255)

                                                 cAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGt                                             >  1:509187/1‑100 (MQ=255)

                                                 cAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGt                                             <  1:97131/100‑1 (MQ=255)

                                                  aGTTTCTATTTATTTGGTGAATGGTATTATGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGtt                                            <  2:1703909/100‑1 (MQ=255)

                                                  aGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGtt                                            <  1:784361/100‑1 (MQ=255)

                                                  aGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGtt                                            <  1:338334/100‑1 (MQ=255)

                                                   gTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGttt                                           <  1:1450581/100‑1 (MQ=255)

                                                   gTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGttt                                           <  1:334918/100‑1 (MQ=255)

                                                    tttCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTa                                          <  2:1456916/100‑1 (MQ=255)

                                                      tCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACa                                        <  1:1080531/100‑1 (MQ=255)

                                                       cTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACaa                                       >  2:152834/1‑100 (MQ=255)

                                                        tatttattTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAg                                      >  2:2169826/1‑100 (MQ=255)

                                                        tatttattTGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAg                                      <  2:849800/100‑1 (MQ=255)

                                                         atttatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGc                                     >  2:920318/1‑100 (MQ=255)

                                                         atttatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGc                                     >  2:1386132/1‑100 (MQ=255)

                                                         atttatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGc                                     <  1:1826140/100‑1 (MQ=255)

                                                         atttatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGc                                     <  1:311264/100‑1 (MQ=255)

                                                         atttatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGc                                     <  1:644652/100‑1 (MQ=255)

                                                          tttatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCa                                    <  1:1029438/100‑1 (MQ=255)

                                                            tatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcg                                  <  1:1550556/100‑1 (MQ=255)

                                                            tatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcg                                  <  2:1200963/100‑1 (MQ=255)

                                                              tttGGTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcgcg                                <  1:1391850/100‑1 (MQ=255)

                                                                tGGGGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAt                              >  2:126994/1‑100 (MQ=255)

                                                                  gTGAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAACACACGGTCAGCCAGATGGTTTACAAGCACGCGAttt                            >  2:1398518/1‑100 (MQ=255)

                                                                    gAATGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCt                          <  2:1446491/100‑1 (MQ=255)

                                                                      aTGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTAc                        >  2:2450990/1‑100 (MQ=255)

                                                                       tGGTATTAAGCTGCTAGGGCAAATCAAGTCTTTTGATCAGTTGGTGATCCTGTTGAAAAAGACGGTCAGGCAGATGGTTTACAAGCACGCGATTTCTAc                        <  1:2627241/99‑1 (MQ=255)

                                                                       tGGTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACt                       <  1:2681723/100‑1 (MQ=255)

                                                                        ggTATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtg                      <  2:596943/100‑1 (MQ=255)

                                                                          tATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtgtt                    >  2:1384949/1‑100 (MQ=255)

                                                                          tATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtgtt                    >  1:288492/1‑100 (MQ=255)

                                                                          tATTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtgtt                    >  2:1497712/1‑100 (MQ=255)

                                                                           aTTAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTATACtgttg                   >  1:1256320/1‑100 (MQ=255)

                                                                            ttAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtgttgt                  >  2:2089130/1‑100 (MQ=255)

                                                                            ttAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtgttgt                  >  2:2152911/1‑100 (MQ=255)

                                                                             tAAGCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTc                 >  2:1034246/1‑100 (MQ=255)

                                                                                gCTGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCg              <  1:2381413/100‑1 (MQ=255)

                                                                                  tGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGtc            <  2:337037/100‑1 (MQ=255)

                                                                                  tGCAAGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGtc            >  2:1277436/1‑100 (MQ=255)

                                                                                     aaGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCg         <  2:2303459/100‑1 (MQ=255)

                                                                                      aGGGCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGc        >  2:2804613/1‑100 (MQ=255)

                                                                                       gggCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGcc       <  1:408461/100‑1 (MQ=255)

                                                                                       gggCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGcc       >  1:1141115/1‑100 (MQ=255)

                                                                                        ggCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGccc      >  1:2167134/1‑100 (MQ=255)

                                                                                        ggCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGccc      >  2:1716132/1‑100 (MQ=255)

                                                                                        ggCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGccc      >  1:1596001/1‑100 (MQ=255)

                                                                                        ggCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGccc      >  1:1586359/1‑100 (MQ=255)

                                                                                        ggCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGccc      <  2:135908/100‑1 (MQ=255)

                                                                                        ggCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGccc      <  2:1145237/100‑1 (MQ=255)

                                                                                         gCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCg     <  2:2536055/100‑1 (MQ=255)

                                                                                         gCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCg     <  1:2594006/100‑1 (MQ=255)

                                                                                         gCAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCg     >  2:1841859/1‑100 (MQ=255)

                                                                                          cAAATCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCgg    >  1:1797078/1‑100 (MQ=255)

                                                                                            aaTCAAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGtt  <  2:1528986/100‑1 (MQ=255)

CAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTT  >  NC_000913/4400300‑4400491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`