BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,308,318	G→C	R110G (CGT→GGT)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,308,318	0	G	C	99.1%	385.0 / ‑3.6	107	R110G (CGT→GGT)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (61/45); total (61/46)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

Only 100 of 108 total aligned reads displayed.

CGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACGACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGT  >  NC_000913/1308223‑1308414

cgcgAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGc                                                                                              >  2:2310545/1‑100 (MQ=255)

    aGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGc                                                                                          >  2:1216222/1‑100 (MQ=255)

     tcTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCa                                                                                         <  1:765417/99‑1 (MQ=255)

     gCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCa                                                                                         >  1:2632011/1‑100 (MQ=255)

     gCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCa                                                                                         >  1:458814/1‑100 (MQ=255)

      cTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCaa                                                                                        >  1:2493009/1‑100 (MQ=255)

        ggATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCaaga                                                                                      >  2:765413/1‑98 (MQ=255)

        ggATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAg                                                                                      >  2:601236/1‑100 (MQ=255)

          aTGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCt                                                                                    <  1:2712926/100‑1 (MQ=255)

           tGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCtt                                                                                   <  1:1691944/100‑1 (MQ=255)

            gTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCtta                                                                                  >  2:1691935/1‑99 (MQ=255)

            gTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTg                                                                                  >  1:2774870/1‑100 (MQ=255)

            gTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTg                                                                                  <  1:2406716/100‑1 (MQ=255)

            gTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTg                                                                                  <  2:249151/100‑1 (MQ=255)

            gTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCAGCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTg                                                                                  >  2:1549245/1‑100 (MQ=255)

             tCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGCCCACGCTCGCAAAGCTTGa                                                                                 >  1:1291330/1‑100 (MQ=255)

             tCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGa                                                                                 >  2:1761888/1‑100 (MQ=255)

              cgcgGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGaa                                                                                >  2:112626/1‑100 (MQ=255)

              cgcgGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGaa                                                                                >  2:1530371/1‑100 (MQ=255)

               gcgGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGaat                                                                               <  2:1168535/100‑1 (MQ=255)

                cgGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGaata                                                                              <  2:2044175/100‑1 (MQ=255)

                    ttAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATgg                                                                          <  2:375226/100‑1 (MQ=255)

                     tAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGgc                                                                         >  2:1300171/1‑100 (MQ=255)

                      aaCGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATg                                                                           >  2:2724125/1‑97 (MQ=255)

                          ccTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAGGCTTGAATAATGGCGCAGc                                                                    >  1:1403645/1‑100 (MQ=255)

                          ccTGCATCACATCATCCGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGc                                                                    >  2:971316/1‑100 (MQ=255)

                            tGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGa                                                                  >  1:1023603/1‑100 (MQ=255)

                             gCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGac                                                                 >  2:2639078/1‑100 (MQ=255)

                              catcacatcaTCTGACTCGGGCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGaca                                                                >  2:1780491/1‑100 (MQ=255)

                               atcacatcaTCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGacac                                                               <  2:2631996/100‑1 (MQ=255)

                                tcacatcaTCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACt                                                              <  1:1549253/100‑1 (MQ=255)

                                  acatcaTCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGc                                                            <  2:1295426/100‑1 (MQ=255)

                                  acatcaTCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGc                                                            >  2:953338/1‑100 (MQ=255)

                                      catcTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTAtt                                                        >  2:1400210/1‑100 (MQ=255)

                                      catcTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACAATGCTAtt                                                        >  1:1086997/1‑100 (MQ=255)

                                       atcTGACTCGGTCATCAGTTGTAGCTGGTGCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTAttt                                                       <  2:2824862/100‑1 (MQ=255)

                                         cTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTttc                                                     >  1:2377073/1‑100 (MQ=255)

                                          tGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTttct                                                    >  1:747750/1‑100 (MQ=255)

                                          tGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTttct                                                    <  2:308191/100‑1 (MQ=255)

                                           gACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTttctt                                                   >  2:2771844/1‑100 (MQ=255)

                                             cTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCg                                                 <  1:2310558/100‑1 (MQ=255)

                                             cTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCg                                                 >  1:2703258/1‑100 (MQ=255)

                                              tCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCgg                                                <  1:459704/100‑1 (MQ=255)

                                               cGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGc                                               >  2:280170/1‑100 (MQ=255)

                                                ggTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCg                                              <  1:601241/100‑1 (MQ=255)

                                                   catcaGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAg                                           >  2:171656/1‑100 (MQ=255)

                                                    atcaGTTGTAGCTGATTCCCTTTGACTCCAGAGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGa                                          <  1:1780500/100‑1 (MQ=255)

                                                      caGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGata                                        <  2:2590786/100‑1 (MQ=255)

                                                        gTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATg                                      >  2:1407511/1‑100 (MQ=255)

                                                         ttGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGATCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGc                                     <  1:2574199/100‑1 (MQ=255)

                                                          tacaacTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTTGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCt                                    <  2:1809807/95‑1 (MQ=255)

                                                          tGTAGCTGATTCCCTTTGACCCCAGCGCTCCCCTGACCACGCTCGCAAAGCTTGCATACTGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCt                                    <  2:938637/100‑1 (MQ=255)

                                                          tGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCt                                    <  2:938718/100‑1 (MQ=255)

                                                           gTAGCTGATTCCCTTTGACCTCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCtt                                   >  2:1727424/1‑100 (MQ=255)

                                                              gCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTTCTATTTTCTTCGGCGAAGATATGCTTACa                                >  1:1784058/1‑100 (MQ=255)

                                                              gCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACa                                <  1:2696960/100‑1 (MQ=255)

                                                               cTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAg                               <  1:472208/100‑1 (MQ=255)

                                                                tGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAgg                              >  1:1807564/1‑100 (MQ=255)

                                                                tGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAgg                              >  2:774938/1‑100 (MQ=255)

                                                                ngATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAgg                              >  1:385069/2‑100 (MQ=255)

                                                                 gATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGc                             <  2:126293/100‑1 (MQ=255)

                                                                  aTTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCt                            >  2:359145/1‑100 (MQ=255)

                                                                   ttCCCTTTGACCCCAGCGATCCCCTGACGACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCtt                           <  2:1878734/100‑1 (MQ=255)

                                                                   ttCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCtt                           <  2:2030403/100‑1 (MQ=255)

                                                                   ttCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCtt                           <  1:112627/100‑1 (MQ=255)

                                                                   ttCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCtt                           <  1:1530379/100‑1 (MQ=255)

                                                                    tCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCttt                          <  1:1400217/100‑1 (MQ=255)

                                                                    tCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCttt                          <  2:598650/100‑1 (MQ=255)

                                                                    tCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCttt                          >  2:2803836/1‑100 (MQ=255)

                                                                    tCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCttt                          >  2:781109/1‑100 (MQ=255)

                                                                     cccTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTa                         >  2:16285/1‑100 (MQ=255)

                                                                     cccTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCCTTa                         <  1:2771861/100‑1 (MQ=255)

                                                                       cTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTATTAGGCGAAGAAATGCTTACAGGCATTAAg                       >  1:264536/1‑100 (MQ=255)

                                                                        tttGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAgg                      <  2:1851362/100‑1 (MQ=255)

                                                                        tttGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTCTCTTCGGCGAAGATATGCTTACAGGCTTTAAg                       <  1:2686247/99‑1 (MQ=255)

                                                                          tGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGtn                    <  2:385066/100‑2 (MQ=255)

                                                                          tGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGt                     >  1:540752/1‑99 (MQ=255)

                                                                          tGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTc                    <  2:1205164/100‑1 (MQ=255)

                                                                           gACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCg                   >  2:67219/1‑100 (MQ=255)

                                                                           gACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCg                   >  1:2308119/1‑100 (MQ=255)

                                                                             ccccAGCGATCCCCTGACCACGCTCTCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGt                  <  1:971322/99‑1 (MQ=255)

                                                                             ccccAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGtt                 >  2:1773652/1‑100 (MQ=255)

                                                                             ccccAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGtt                 >  2:2484945/1‑100 (MQ=255)

                                                                              cccAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTa                >  1:1480447/1‑100 (MQ=255)

                                                                              cccAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTa                <  1:1804795/100‑1 (MQ=255)

                                                                                cAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAg              <  2:2097525/100‑1 (MQ=255)

                                                                                 aGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGc             >  1:34226/1‑100 (MQ=255)

                                                                                 aGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAAGCTTTAAGGTCGTTAAGc             >  1:1673811/1‑100 (MQ=255)

                                                                                  gCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGcc            >  2:2051962/1‑100 (MQ=255)

                                                                                  gCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGcc            >  2:625044/1‑100 (MQ=255)

                                                                                   cGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCa           >  1:2566332/1‑100 (MQ=255)

                                                                                      tCCCGTGACCACGCTCGCAAAGCGTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGTGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCAttt        <  2:2752762/100‑1 (MQ=255)

                                                                                      tCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCAttt        <  2:1023597/100‑1 (MQ=255)

                                                                                      tCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCAttt        >  2:874450/1‑100 (MQ=255)

                                                                                       ccccTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCAtttt       <  2:2774853/100‑1 (MQ=255)

                                                                                        cccTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTg      <  2:2492995/100‑1 (MQ=255)

                                                                                         ccTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGc     >  1:2550216/1‑100 (MQ=255)

                                                                                         ccTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGc     >  2:342815/1‑100 (MQ=255)

                                                                                           tGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCgg   >  2:145808/1‑100 (MQ=255)

                                                                                            gaccacGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGt  >  2:419830/1‑100 (MQ=255)

CGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACGACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGT  >  NC_000913/1308223‑1308414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`