Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,499,369	C→T	17.9%	V144V (GTC→GTT)	rimL →	ribosomal‑protein‑L7/L12‑serine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,499,369	0	C	T	17.9%	201.1 / 33.9	90	V144V (GTC→GTT)	rimL	ribosomal‑protein‑L7/L12‑serine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (37/36);  new base T (8/8);  total (46/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.74e-01

CAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGAT  >  NC_000913/1499271‑1499468                                                                                                   |                                                                                                    cAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCg                                                                                                    >  2:621137/1‑100 (MQ=255)     cgcTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCttt                                                                                                >  1:1130007/1‑100 (MQ=255)        tGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcg                                                                                             <  1:2288318/100‑1 (MQ=255)        tGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcg                                                                                             <  2:407407/100‑1 (MQ=255)        tGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcg                                                                                             <  2:2343016/100‑1 (MQ=255)        tGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcg                                                                                             <  2:2049840/100‑1 (MQ=255)        tGCAGGCAGTGATTCATCATTATGCCCAGTCTGGTTAACTTATACTCTTCGTGATCAAATGTCGGGTGGACAATCCTCAAAGCAACCAGGTCGCTTTgcg                                                                                             <  2:260742/100‑1 (MQ=255)         gtaGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTgcgc                                                                                            <  2:1670221/98‑1 (MQ=255)         gcatgcatTGATTCATCATTACGCCAAGTCTGGTGAACTTAGACGATTCGTGATCAAATGTCGGGTGGACAAGCCGCAAAGCAACCAGGTCGCTTTgcgc                                                                                            <  2:270896/100‑1 (MQ=255)         gCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATGAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcgg                                                                                            <  1:1194982/100‑2 (MQ=38)         gCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcgg                                                                                            <  1:2467571/100‑2 (MQ=39)         gCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcgg                                                                                            <  1:1488981/100‑2 (MQ=39)           aGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcggat                                                                                          <  1:1366121/100‑4 (MQ=38)              cATTGATTCATCATTACGCCCCGTCTGGTGAACTTACCCGCTTCGTTATCAAATGTCGGGTGGACAATCCGCGAAGCAACCAGGTCGTTTTGCGCAATgg                                                                                       <  2:2157464/100‑1 (MQ=255)                 tGATTCATCATTACGCCCAGTCTGGTGAAGTTAGACGCGTCGTGATCAAATGTCGGGTTCACAATCCGCAAATCAACCCGGTCGCTTTGCGCAATGGttt                                                                                    <  2:2621602/100‑1 (MQ=255)                  gATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGtttt                                                                                   >  2:2566932/1‑100 (MQ=255)                   aTTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTa                                                                                  >  1:1126020/1‑100 (MQ=255)                    ttCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTAt                                                                                 >  2:877159/1‑100 (MQ=255)                     tcatcaTTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATc                                                                                <  1:2494224/100‑1 (MQ=255)                         catTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTg                                                                            <  1:621139/100‑1 (MQ=255)                          atTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGa                                                                           >  1:1609071/1‑100 (MQ=255)                             aCGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAgg                                                                        <  2:2431936/100‑1 (MQ=255)                             aCGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAgg                                                                        >  2:1151801/1‑100 (MQ=255)                              cGCCCAGTCTGGTGAACTTANACGCTTNGTGAGCANATGTCGGGTGGACNATCCGCAAAGCAACCAGGTCGCTTTGCGNAATNGTTTTATCCTTGAAGGt                                                                       >  2:1781089/1‑100 (MQ=255)                              cGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGt                                                                       >  2:254135/1‑100 (MQ=255)                                 ccAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGc                                                                    >  1:1846295/1‑100 (MQ=255)                                  cAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGcc                                                                   <  1:2138890/100‑1 (MQ=255)                                    gTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTg                                                                 <  1:437553/100‑1 (MQ=255)                                     tCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGa                                                                <  2:1047735/100‑1 (MQ=255)                                        ggTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAAc                                                             >  2:298031/1‑100 (MQ=255)                                        ggTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAAc                                                             >  2:717400/1‑100 (MQ=255)                                        ggTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAAc                                                             >  1:1448231/1‑100 (MQ=255)                                        ggTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAAc                                                             >  2:2588836/1‑100 (MQ=255)                                             aCTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATGCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAgg                                                          >  2:1473492/1‑98 (MQ=255)                                             aCTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCt                                                        <  2:1221069/100‑1 (MQ=255)                                              cTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTg                                                       >  2:569719/1‑100 (MQ=255)                                              cTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATACGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTg                                                       >  1:1636343/1‑100 (MQ=255)                                                tAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAg                                                     >  1:365713/1‑100 (MQ=255)                                                tAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAg                                                     >  1:1207670/1‑100 (MQ=255)                                                  gACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGtt                                                   >  1:825223/1‑100 (MQ=255)                                                  gACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGtt                                                   >  1:1174812/1‑100 (MQ=255)                                                    cGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTcc                                                 <  1:572184/100‑1 (MQ=255)                                                      cTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTg                                               >  1:1125610/1‑100 (MQ=255)                                                      cTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTg                                               <  1:2439142/100‑1 (MQ=255)                                                      cTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTg                                               <  2:272337/100‑1 (MQ=255)                                                      cTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTg                                               <  2:2635437/100‑1 (MQ=255)                                                       ttCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCTCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGa                                              <  2:1990990/100‑1 (MQ=255)                                                        tCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGaa                                             <  2:914287/100‑1 (MQ=255)                                                        tCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGaa                                             <  1:229842/100‑1 (MQ=255)                                                        tCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGaa                                             <  1:873724/100‑1 (MQ=255)                                                         cGTGATCAAATGTCGTGTGTACAATCCGAAAAGCAACCAGGTCGCTTTTCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAat                                            <  2:2267113/100‑1 (MQ=255)                                                         cGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAat                                            <  1:1639146/100‑1 (MQ=255)                                                          gTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatg                                           <  2:1058659/100‑1 (MQ=255)                                                           tGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatga                                          >  2:2466689/1‑100 (MQ=255)                                                             aTCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatgatg                                        >  1:1628620/1‑100 (MQ=255)                                                             aTCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatgatg                                        >  2:185529/1‑100 (MQ=255)                                                                    gTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatga                                 >  1:2106637/1‑100 (MQ=255)                                                                     tCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgat                                <  1:2466705/100‑1 (MQ=255)                                                                      cGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCTCAATGGTGTTATCCTGGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgatg                               <  1:911428/100‑1 (MQ=255)                                                                      cGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgat                                <  2:1397933/99‑1 (MQ=255)                                                                         gTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgatgatg                            <  2:121844/100‑1 (MQ=255)                                                                             ccattgcGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGaa                        >  1:1593355/7‑100 (MQ=38)                                                                             aCAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGaa                        >  2:5577/1‑100 (MQ=255)                                                                             aCAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGaa                        >  1:840748/1‑100 (MQ=255)                                                                             aCAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGaa                        >  1:456368/1‑100 (MQ=255)                                                                              cAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAAc                       >  1:221782/1‑100 (MQ=255)                                                                              cAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAAc                       >  1:615054/1‑100 (MQ=255)                                                                               attgagCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATTATGTGCACt                      >  2:1118345/6‑100 (MQ=38)                                                                               aaTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACt                      >  2:1581777/1‑100 (MQ=255)                                                                               aaTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACt                      <  1:2175474/100‑1 (MQ=255)                                                                               aaTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTCTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACt                      <  2:835624/100‑1 (MQ=255)                                                                                aTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACtt                     <  1:1675489/100‑1 (MQ=255)                                                                                  gcGCAAAGCGACCTGGTTGCTTTGTGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtat                   >  2:1373396/2‑100 (MQ=255)                                                                                  gcGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtat                   >  1:1776451/2‑100 (MQ=39)                                                                                   cGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata                  >  1:464109/1‑100 (MQ=255)                                                                                   cGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata                  >  1:2087197/1‑100 (MQ=255)                                                                                   cGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata                  <  1:1266294/100‑1 (MQ=255)                                                                                   cGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata                  <  1:298031/100‑1 (MQ=255)                                                                                    gCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAc                 >  1:1345630/1‑100 (MQ=255)                                                                                    gCAAAGCAACCAGGTCGCGTTGCGCAATGGTGTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata                  <  2:1554565/99‑1 (MQ=255)                                                                                     cAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcg                >  1:2693361/1‑100 (MQ=255)                                                                                       aaGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcgcg              >  1:239817/1‑100 (MQ=255)                                                                                          cAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGt           >  2:1905149/1‑100 (MQ=255)                                                                                          cAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGt           >  1:1577976/1‑100 (MQ=255)                                                                                            aCCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtat         <  1:934165/100‑1 (MQ=255)                                                                                            aCCAGGTAGCTATGCGCAATGATTTTATCCTTGAAAGTTGCCTGAAACAGACTGAGTTCCTGCATCATGGCTATGATGATGTGACCGTATAtgcgag            >  2:1441401/1‑95 (MQ=255)                                                                                               aGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtattat      <  2:2116990/100‑1 (MQ=255)                                                                                               aGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtattat      <  2:416933/100‑1 (MQ=255)                                                                                                ggTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATc     >  1:1557886/1‑100 (MQ=255)                                                                                                 gTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCg    >  1:1832722/1‑100 (MQ=255)                                                                                                   cGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGAt  <  1:2305254/100‑1 (MQ=255)                                                                                                   cGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGAt  <  2:2168126/100‑1 (MQ=255)                                                                                                   cGCTTTGCGCAAGGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATTATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGAt  <  2:151147/100‑1 (MQ=255)                                                                                                   |                                                                                                    CAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGAT  >  NC_000913/1499271‑1499468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑