Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,306,788 T→C 15.8% intergenic (+20/+33) oppF → / ← yciU oligopeptide ABC transporter ATPase/UPF0263 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,306,7880TC15.8% 279.9 / 42.8 121intergenic (+20/+33)oppF/yciUoligopeptide ABC transporter ATPase/UPF0263 family protein
Reads supporting (aligned to +/- strand):  ref base T (64/37);  new base C (13/6);  total (77/44)
Fisher's exact test for biased strand distribution p-value = 7.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

Only 100 of 122 total aligned reads displayed.

CAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCA  >  NC_000913/1306689‑1306884
                                                                                                   |                                                                                                
cAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGt                                                                                                  >  1:418881/1‑100 (MQ=255)
    gTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTc                                                                                              <  1:2484700/100‑1 (MQ=255)
    gTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTc                                                                                              <  1:2607692/100‑1 (MQ=255)
     tGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCa                                                                                             <  2:899232/100‑1 (MQ=255)
       cgcCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGc                                                                                           <  1:945049/100‑1 (MQ=255)
        gcCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAgat                                                                                          >  1:943553/1‑98 (MQ=255)
        gcCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGcc                                                                                          >  2:1171212/1‑100 (MQ=255)
        gcCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGcc                                                                                          >  2:1116677/1‑100 (MQ=255)
         ccAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGccc                                                                                         >  1:208678/1‑100 (MQ=255)
         ccAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGccc                                                                                         >  2:63166/1‑100 (MQ=255)
         ccAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGccc                                                                                         >  1:1316221/1‑100 (MQ=255)
          cAAAACACGTCCTGTTCTGGAGGGGAGTTTGAGACACTCCGTTTCATGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCt                                                                                        <  2:637785/100‑1 (MQ=255)
          cAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCt                                                                                        <  1:1270671/100‑1 (MQ=255)
          cAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCt                                                                                        <  2:2060953/100‑1 (MQ=255)
          cAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCt                                                                                        <  1:2026964/100‑1 (MQ=255)
          cAAAACACGTCCTGTTCTGGAGAGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCt                                                                                        <  2:2013299/100‑1 (MQ=255)
           aaaaCACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCtt                                                                                       >  2:566773/1‑100 (MQ=255)
               cacGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTc                                                                                   >  1:138027/1‑100 (MQ=255)
                   tCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGc                                                                               >  2:2591454/1‑100 (MQ=255)
                       gTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTaaa                                                                           >  1:2032795/1‑100 (MQ=255)
                       gTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTCTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTaaa                                                                           <  2:1522308/100‑1 (MQ=255)
                            ggAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGa                                                                      >  1:1686878/1‑100 (MQ=255)
                             gAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGat                                                                     >  2:2704536/1‑100 (MQ=255)
                               ggggAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAACCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGatta                                                                   <  2:2452415/100‑1 (MQ=255)
                                 ggAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGattatt                                                                 <  1:712706/100‑1 (MQ=255)
                                 ggAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGattatt                                                                 <  2:83558/100‑1 (MQ=255)
                                    gTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGAttatttat                                                              <  2:1476604/100‑1 (MQ=255)
                                    gTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGAttatttat                                                              >  1:378694/1‑100 (MQ=255)
                                      ttCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTc                                                            >  2:2188534/1‑100 (MQ=255)
                                           acacTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGcc                                                       <  1:1244739/100‑1 (MQ=255)
                                             acTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAg                                                     <  2:2704981/100‑1 (MQ=255)
                                             acTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAg                                                     >  2:2622188/1‑100 (MQ=255)
                                             acTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAg                                                     >  1:445720/1‑100 (MQ=255)
                                              cTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAga                                                    >  2:1414424/1‑100 (MQ=255)
                                               tCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgat                                                   >  1:1847729/1‑100 (MQ=255)
                                               tCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgat                                                   >  2:553901/1‑100 (MQ=255)
                                                 cGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatga                                                 >  1:2591002/1‑100 (MQ=255)
                                                    ttCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGatat                                              <  2:418880/100‑1 (MQ=255)
                                                     tCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATg                                             >  1:2547210/1‑100 (MQ=255)
                                                      cTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATgg                                            >  2:1485404/1‑100 (MQ=255)
                                                      cTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATgg                                            <  1:1223854/100‑1 (MQ=255)
                                                      cTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATgg                                            >  2:2266549/1‑100 (MQ=255)
                                                       ttGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGc                                           <  2:715715/100‑1 (MQ=255)
                                                         gacTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTGGGCAGCCATTTTTCACGCAAAAAGCGATTACTTATTCCGGCCATATGatattgaaa                                         >  2:2362477/3‑95 (MQ=255)
                                                         gCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCaa                                         >  2:2361157/1‑100 (MQ=255)
                                                         gCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCaa                                         <  2:577196/100‑1 (MQ=255)
                                                         gCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCaa                                         >  1:2719050/1‑100 (MQ=255)
                                                          ccTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCaaa                                        >  1:601590/1‑100 (MQ=255)
                                                          ccTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCaaa                                        >  2:174701/1‑100 (MQ=255)
                                                           cTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAg                                       <  1:840392/100‑1 (MQ=255)
                                                             gAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGtt                                     <  1:88170/100‑1 (MQ=255)
                                                               aaGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTtg                                   >  1:1608377/1‑100 (MQ=255)
                                                                aGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTtgt                                  >  1:78387/1‑100 (MQ=255)
                                                                aGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTtgt                                  <  1:111936/100‑1 (MQ=255)
                                                                aGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTtg                                   <  1:2467878/99‑1 (MQ=255)
                                                                 gTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTtgtg                                 <  2:2234556/100‑1 (MQ=255)
                                                                 gTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTtgtg                                 <  1:2003383/100‑1 (MQ=255)
                                                                 gTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTtgtg                                 >  2:2659461/1‑100 (MQ=255)
                                                                 gTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCAAAAAGCGATTATTTATTACCGCCAGATGATCTGGCACCGttt                                    >  1:227525/1‑97 (MQ=255)
                                                                  tCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGa                                >  1:582019/1‑100 (MQ=255)
                                                                    gATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATc                              >  2:2529305/1‑100 (MQ=255)
                                                                    gATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATc                              >  2:2647826/1‑100 (MQ=255)
                                                                     aTCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCt                             >  1:790769/1‑100 (MQ=255)
                                                                     aTCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCt                             >  2:2557772/1‑100 (MQ=255)
                                                                      tCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCtt                            >  2:443361/1‑100 (MQ=255)
                                                                      tCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCtt                            >  1:2228877/1‑100 (MQ=255)
                                                                        cGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCtttt                          >  1:2212552/1‑100 (MQ=255)
                                                                        cGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCtttt                          >  1:2107439/1‑100 (MQ=255)
                                                                         gCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCttttt                         <  2:2085342/100‑1 (MQ=255)
                                                                         gCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCttttt                         <  1:2055452/100‑1 (MQ=255)
                                                                          cTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTc                        <  1:2139232/100‑1 (MQ=255)
                                                                           tttAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTcg                       >  2:2569036/1‑100 (MQ=255)
                                                                             tAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTcgcg                     >  1:302543/1‑100 (MQ=255)
                                                                              aaaaaCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCgg                    >  1:1518343/1‑100 (MQ=255)
                                                                              aaaaaCAATAAGGGCAGACAGTTGTCAGCCCTTTTTCACCCTAAAAGCGATTATTTATTCCCGCCAGATGATCTGGCAACGTTTGTGATCTTTTTCGCgg                    >  2:2345980/1‑100 (MQ=255)
                                                                               aaaaCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGc                   >  2:1381035/1‑100 (MQ=255)
                                                                               aaaaCAATAAGGGCTGACAGTTGTCAGCCCATTCTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCGTTTTCGCGGc                   >  2:1803125/1‑100 (MQ=255)
                                                                                cgtgaaaaaGGGCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCa                  >  1:1342541/8‑100 (MQ=38)
                                                                                aaaCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCa                  >  2:2047129/1‑100 (MQ=255)
                                                                                aaaCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCa                  >  1:659578/1‑100 (MQ=255)
                                                                                aaaCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCa                  >  1:2309323/1‑100 (MQ=255)
                                                                                  aCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATa                <  2:928163/100‑1 (MQ=255)
                                                                                   cAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATaa               <  1:2386155/100‑1 (MQ=255)
                                                                                    aaTAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCATATGATATGGCAAATTTTGTGATTTTTTTCGCGGCATAAc              <  2:2186920/100‑1 (MQ=255)
                                                                                     aaaaGGGCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCAt                 >  2:150879/3‑96 (MQ=255)
                                                                                     aaaaGGGCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACa             >  1:383721/3‑100 (MQ=255)
                                                                                      aaaGGGCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAg            >  1:1360768/2‑100 (MQ=255)
                                                                                       aaGGGCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGa           >  2:2106863/1‑100 (MQ=255)
                                                                                        aGGGCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGaa          >  1:649746/1‑100 (MQ=255)
                                                                                        aGGGCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGaa          >  1:274213/1‑100 (MQ=255)
                                                                                        aGGGCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGaa          >  1:2087722/1‑100 (MQ=255)
                                                                                        aGGGCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTCTGCGATCTTTTTctcagtata                >  2:215005/1‑86 (MQ=255)
                                                                                         gggCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAAt         >  1:2318542/1‑100 (MQ=255)
                                                                                          ggCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATg        >  2:2227021/1‑100 (MQ=255)
                                                                                           gCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGc       >  1:1065959/1‑100 (MQ=255)
                                                                                            cTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCg      >  2:479015/1‑100 (MQ=255)
                                                                                            cTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCg      >  1:1063626/1‑100 (MQ=255)
                                                                                                cAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATTGCAAAGTTTCTGATCTTTTTCGCGGCATAACAGAATGCTGGCa  >  2:1730798/1‑100 (MQ=255)
                                                                                                cAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCa  >  2:289780/1‑100 (MQ=255)
                                                                                                cAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCa  >  1:168233/1‑100 (MQ=255)
                                                                                                   |                                                                                                
CAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCA  >  NC_000913/1306689‑1306884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: