Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,499,365	A→T	18.6%	Q143L (CAG→CTG)	rimL →	ribosomal‑protein‑L7/L12‑serine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,499,365	0	A	T	18.6%	197.8 / 42.0	87	Q143L (CAG→CTG)	rimL	ribosomal‑protein‑L7/L12‑serine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (36/34);  new base T (8/8);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.33e-01

TTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTAT  >  NC_000913/1499267‑1499464                                                                                                   |                                                                                                    ttCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAg                                                                                                    <  1:2119303/100‑1 (MQ=255)     cAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCg                                                                                                >  2:621137/1‑100 (MQ=255)         cgcTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCttt                                                                                            >  1:1130007/1‑100 (MQ=255)            tGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcg                                                                                         <  2:2049840/100‑1 (MQ=255)            tGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcg                                                                                         <  2:407407/100‑1 (MQ=255)            tGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcg                                                                                         <  2:2343016/100‑1 (MQ=255)            tGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcg                                                                                         <  1:2288318/100‑1 (MQ=255)            tGCAGGCAGTGATTCATCATTATGCCCAGTCTGGTTAACTTATACTCTTCGTGATCAAATGTCGGGTGGACAATCCTCAAAGCAACCAGGTCGCTTTgcg                                                                                         <  2:260742/100‑1 (MQ=255)             gtaGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTgcgc                                                                                        <  2:1670221/98‑1 (MQ=255)             gcatgcatTGATTCATCATTACGCCAAGTCTGGTGAACTTAGACGATTCGTGATCAAATGTCGGGTGGACAAGCCGCAAAGCAACCAGGTCGCTTTgcgc                                                                                        <  2:270896/100‑1 (MQ=255)             gCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATGAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcgg                                                                                        <  1:1194982/100‑2 (MQ=38)             gCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcgg                                                                                        <  1:1488981/100‑2 (MQ=39)             gCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcgg                                                                                        <  1:2467571/100‑2 (MQ=39)               aGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcggat                                                                                      <  1:1366121/100‑4 (MQ=38)                  cATTGATTCATCATTACGCCCCGTCTGGTGAACTTACCCGCTTCGTTATCAAATGTCGGGTGGACAATCCGCGAAGCAACCAGGTCGTTTTGCGCAATgg                                                                                   <  2:2157464/100‑1 (MQ=255)                     tGATTCATCATTACGCCCAGTCTGGTGAAGTTAGACGCGTCGTGATCAAATGTCGGGTTCACAATCCGCAAATCAACCCGGTCGCTTTGCGCAATGGttt                                                                                <  2:2621602/100‑1 (MQ=255)                      gATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGtttt                                                                               >  2:2566932/1‑100 (MQ=255)                       aTTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTa                                                                              >  1:1126020/1‑100 (MQ=255)                        ttCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTAt                                                                             >  2:877159/1‑100 (MQ=255)                         tcatcaTTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATc                                                                            <  1:2494224/100‑1 (MQ=255)                             catTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTg                                                                        <  1:621139/100‑1 (MQ=255)                              atTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGa                                                                       >  1:1609071/1‑100 (MQ=255)                                 aCGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAgg                                                                    <  2:2431936/100‑1 (MQ=255)                                 aCGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAgg                                                                    >  2:1151801/1‑100 (MQ=255)                                  cGCCCAGTCTGGTGAACTTANACGCTTNGTGAGCANATGTCGGGTGGACNATCCGCAAAGCAACCAGGTCGCTTTGCGNAATNGTTTTATCCTTGAAGGt                                                                   >  2:1781089/1‑100 (MQ=255)                                  cGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGt                                                                   >  2:254135/1‑100 (MQ=255)                                     ccAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGc                                                                >  1:1846295/1‑100 (MQ=255)                                      cAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGcc                                                               <  1:2138890/100‑1 (MQ=255)                                        gTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTg                                                             <  1:437553/100‑1 (MQ=255)                                         tCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGa                                                            <  2:1047735/100‑1 (MQ=255)                                            ggTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAAc                                                         >  2:2588836/1‑100 (MQ=255)                                            ggTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAAc                                                         >  1:1448231/1‑100 (MQ=255)                                            ggTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAAc                                                         >  2:717400/1‑100 (MQ=255)                                            ggTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAAc                                                         >  2:298031/1‑100 (MQ=255)                                                 aCTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATGCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAgg                                                      >  2:1473492/1‑98 (MQ=255)                                                 aCTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCt                                                    <  2:1221069/100‑1 (MQ=255)                                                  cTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTg                                                   >  2:569719/1‑100 (MQ=255)                                                  cTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATACGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTg                                                   >  1:1636343/1‑100 (MQ=255)                                                    tAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAg                                                 >  1:365713/1‑100 (MQ=255)                                                    tAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAg                                                 >  1:1207670/1‑100 (MQ=255)                                                      gACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGtt                                               >  1:1174812/1‑100 (MQ=255)                                                      gACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGtt                                               >  1:825223/1‑100 (MQ=255)                                                        cGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTcc                                             <  1:572184/100‑1 (MQ=255)                                                          cTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTg                                           >  1:1125610/1‑100 (MQ=255)                                                          cTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTg                                           <  2:272337/100‑1 (MQ=255)                                                          cTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTg                                           <  2:2635437/100‑1 (MQ=255)                                                          cTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTg                                           <  1:2439142/100‑1 (MQ=255)                                                           ttCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCTCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGa                                          <  2:1990990/100‑1 (MQ=255)                                                            tCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGaa                                         <  2:914287/100‑1 (MQ=255)                                                            tCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGaa                                         <  1:873724/100‑1 (MQ=255)                                                            tCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGaa                                         <  1:229842/100‑1 (MQ=255)                                                             cGTGATCAAATGTCGTGTGTACAATCCGAAAAGCAACCAGGTCGCTTTTCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAat                                        <  2:2267113/100‑1 (MQ=255)                                                             cGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAat                                        <  1:1639146/100‑1 (MQ=255)                                                              gTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatg                                       <  2:1058659/100‑1 (MQ=255)                                                               tGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatga                                      >  2:2466689/1‑100 (MQ=255)                                                                 aTCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatgatg                                    >  2:185529/1‑100 (MQ=255)                                                                 aTCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatgatg                                    >  1:1628620/1‑100 (MQ=255)                                                                        gTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatga                             >  1:2106637/1‑100 (MQ=255)                                                                         tCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgat                            <  1:2466705/100‑1 (MQ=255)                                                                          cGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCTCAATGGTGTTATCCTGGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgatg                           <  1:911428/100‑1 (MQ=255)                                                                          cGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgat                            <  2:1397933/99‑1 (MQ=255)                                                                             gTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgatgatg                        <  2:121844/100‑1 (MQ=255)                                                                                 ccattgcGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGaa                    >  1:1593355/7‑100 (MQ=38)                                                                                 aCAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGaa                    >  1:840748/1‑100 (MQ=255)                                                                                 aCAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGaa                    >  2:5577/1‑100 (MQ=255)                                                                                 aCAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGaa                    >  1:456368/1‑100 (MQ=255)                                                                                  cAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAAc                   >  1:221782/1‑100 (MQ=255)                                                                                  cAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAAc                   >  1:615054/1‑100 (MQ=255)                                                                                   attgagCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATTATGTGCACt                  >  2:1118345/6‑100 (MQ=38)                                                                                   aaTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACt                  >  2:1581777/1‑100 (MQ=255)                                                                                   aaTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACt                  <  1:2175474/100‑1 (MQ=255)                                                                                   aaTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTCTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACt                  <  2:835624/100‑1 (MQ=255)                                                                                    aTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACtt                 <  1:1675489/100‑1 (MQ=255)                                                                                      gcGCAAAGCGACCTGGTTGCTTTGTGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtat               >  2:1373396/2‑100 (MQ=255)                                                                                      gcGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtat               >  1:1776451/2‑100 (MQ=39)                                                                                       cGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata              >  1:464109/1‑100 (MQ=255)                                                                                       cGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata              >  1:2087197/1‑100 (MQ=255)                                                                                       cGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata              <  1:1266294/100‑1 (MQ=255)                                                                                       cGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata              <  1:298031/100‑1 (MQ=255)                                                                                        gCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAc             >  1:1345630/1‑100 (MQ=255)                                                                                        gCAAAGCAACCAGGTCGCGTTGCGCAATGGTGTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata              <  2:1554565/99‑1 (MQ=255)                                                                                         cAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcg            >  1:2693361/1‑100 (MQ=255)                                                                                           aaGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcgcg          >  1:239817/1‑100 (MQ=255)                                                                                              cAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGt       >  2:1905149/1‑100 (MQ=255)                                                                                              cAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGt       >  1:1577976/1‑100 (MQ=255)                                                                                                aCCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtat     <  1:934165/100‑1 (MQ=255)                                                                                                aCCAGGTAGCTATGCGCAATGATTTTATCCTTGAAAGTTGCCTGAAACAGACTGAGTTCCTGCATCATGGCTATGATGATGTGACCGTATAtgcgag        >  2:1441401/1‑95 (MQ=255)                                                                                                   aGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtattat  <  2:416933/100‑1 (MQ=255)                                                                                                   aGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtattat  <  2:2116990/100‑1 (MQ=255)                                                                                                   |                                                                                                    TTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTAT  >  NC_000913/1499267‑1499464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑