Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,950,302 Δ1 bp 15.1% coding (221/1773 nt) aspS ← aspartyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,950,3010G.15.1% 432.0 / 79.0 146coding (222/1773 nt)aspSaspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (61/61);  new base . (14/8);  total (77/69)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01

Only 100 of 148 total aligned reads displayed.

ACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTC  >  NC_000913/1950203‑1950399
                                                                                                   |                                                                                                  
aCATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACg                                                                                                    <  2:3122829/100‑1 (MQ=255)
   tCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGc                                                                                                <  1:622506/100‑1 (MQ=255)
    cTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTgac                                                                                               >  1:233748/1‑98 (MQ=255)
    cTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGcc                                                                                               <  1:2768388/100‑1 (MQ=255)
    cTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGcc                                                                                               >  2:2859947/1‑100 (MQ=255)
     tGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGccc                                                                                              <  1:406110/100‑1 (MQ=255)
         cgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACGTGCGCGTGa                                                                                          <  1:1572083/100‑1 (MQ=38)
         cgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGa                                                                                          >  2:3257869/1‑100 (MQ=255)
          ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACGTGCGCGTGAc                                                                                         <  1:227702/100‑1 (MQ=38)
          ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGAc                                                                                         <  2:3644544/100‑1 (MQ=255)
          ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGAc                                                                                         <  2:2944384/100‑1 (MQ=255)
          ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGAc                                                                                         >  2:3540029/1‑100 (MQ=255)
          ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGACCGTGa                                                                                          >  1:1332621/1‑99 (MQ=255)
          ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGCGCCCGTGAc                                                                                         >  2:3540019/1‑100 (MQ=255)
           gTTGATGATTGTCAGCGCGGACGCCAGCTCTTCGATTTCGCCGGTCGCCATATCGCGGTTGATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTgcc                                                                                         <  1:2602981/99‑3 (MQ=255)
           gTTGATGATAGTCAGCGAGGACGCCCGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACGTGCGCGTGAcg                                                                                        <  2:645759/100‑2 (MQ=37)
           gTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACGTGCGCGTGAcg                                                                                        <  2:532251/100‑2 (MQ=38)
           gTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACGTGCGCGTGAcg                                                                                        <  1:983416/100‑2 (MQ=38)
           gTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGAcc                                                                                        >  2:451763/1‑100 (MQ=255)
                tgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTTTTTACTCTCACGTACGGTGCCCGTGACCTGAAt                                                                                   >  1:2707293/1‑100 (MQ=255)
                 gatAGTCAGCGAGGACGCCAGCACTTCGATTTGGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACGTGCGCGTGAcgaaaaaa                                                                                  <  2:1425216/100‑8 (MQ=25)
                 gatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATg                                                                                  <  1:2604877/100‑1 (MQ=255)
                 gatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATg                                                                                  <  1:2864170/100‑1 (MQ=255)
                  atAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGc                                                                                 <  2:2756945/100‑1 (MQ=255)
                   tAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCa                                                                                >  1:1558000/1‑100 (MQ=255)
                   tAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTGCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCa                                                                                <  1:1199549/100‑1 (MQ=255)
                    aGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAGTATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAg                                                                               <  1:3235820/100‑1 (MQ=255)
                     gTCAGCGAGTACGCCAGCACTTCGTTTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCTTCACCCGCACGTACGGTGCCCGTGTCCTGAATGCAGa                                                                              <  1:2434712/100‑1 (MQ=255)
                     gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGCTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGa                                                                              <  2:615211/100‑1 (MQ=255)
                      tCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGaa                                                                             <  2:1671023/100‑1 (MQ=255)
                       cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                            <  2:1978231/100‑1 (MQ=255)
                       cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                            <  2:632961/100‑1 (MQ=255)
                       cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                            >  1:424946/1‑100 (MQ=255)
                       cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                            >  2:2958988/1‑100 (MQ=255)
                       cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                            >  1:1264691/1‑100 (MQ=255)
                        aGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACt                                                                           >  1:1469474/1‑100 (MQ=255)
                         gCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTc                                                                          >  2:89845/1‑100 (MQ=255)
                             ggACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTa                                                                      <  1:502598/100‑1 (MQ=255)
                             ggACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTa                                                                      >  2:1061371/1‑100 (MQ=255)
                                  ccAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAg                                                                 >  2:1430446/1‑100 (MQ=255)
                                  ccAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAg                                                                 <  2:3593434/100‑1 (MQ=255)
                                  ccAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACCGTGCCCGTGACCTGAATGCAGAACTCATTACGc                                                                   >  2:3609284/1‑98 (MQ=255)
                                    aGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGtt                                                               >  2:939987/1‑100 (MQ=255)
                                     gCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTc                                                              <  2:3576159/100‑1 (MQ=255)
                                        cTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCaga                                                           >  2:556717/1‑100 (MQ=255)
                                        cTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCaga                                                           >  2:1850503/1‑100 (MQ=255)
                                        cTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCaga                                                           >  2:1235072/1‑100 (MQ=255)
                                         ttCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCagag                                                          <  2:2475196/100‑1 (MQ=255)
                                         ttCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCagag                                                          <  1:3759021/100‑1 (MQ=255)
                                         ttCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTATAAGCAGAACTCATTACGCAGCTccaa                                                           >  1:2705242/1‑96 (MQ=255)
                                          tCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAgg                                                         >  2:3799814/1‑100 (MQ=255)
                                          tCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAgg                                                         <  1:2002599/100‑1 (MQ=255)
                                          tCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAgg                                                         >  1:1929744/1‑100 (MQ=255)
                                              tttCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAg                                                     <  2:3062018/100‑1 (MQ=255)
                                               ttCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGc                                                    <  1:1850494/100‑1 (MQ=255)
                                                 cGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCtt                                                  >  1:3567412/1‑100 (MQ=255)
                                                 cGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCtt                                                  <  2:1264699/100‑1 (MQ=255)
                                                 cGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCtt                                                  >  2:2810014/1‑100 (MQ=255)
                                                 cGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCtt                                                  >  2:3574017/1‑100 (MQ=255)
                                                 cGCCGGTCGCCATATCGCGGTGAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCtt                                                  <  1:3366903/100‑1 (MQ=255)
                                                 cGCCGGTCGCCATATCACGGTTAATATTTTTTTCGTCACGCGCCCGTACGGTGCCGGTAACCTCAATAGAGAAATCATTACGCAGTAAAGAGGCCAGCt                                                   >  1:799948/1‑99 (MQ=255)
                                                  gCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCttt                                                 >  2:970118/1‑100 (MQ=255)
                                                   ccGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTa                                                <  2:131710/100‑1 (MQ=255)
                                                   ccGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTa                                                <  2:3418716/100‑1 (MQ=255)
                                                    cGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTaa                                               <  1:1110263/100‑1 (MQ=255)
                                                    cGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTaa                                               <  1:1110149/100‑1 (MQ=255)
                                                      gTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGATGCCAGCTTTaatg                                             <  2:2612090/100‑3 (MQ=255)
                                                        cGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAcgcg                                           <  2:2145732/100‑1 (MQ=255)
                                                           cATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTcc                                        >  2:70474/1‑100 (MQ=255)
                                                              atCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCa                                     >  2:2197672/1‑100 (MQ=255)
                                                                 gcgGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGa                                  >  2:851064/1‑100 (MQ=255)
                                                                  cgGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGAt                                 >  1:2934203/1‑100 (MQ=255)
                                                                    gTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATcc                               <  2:3248375/100‑1 (MQ=255)
                                                                         tatTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATc                          >  2:2226926/1‑100 (MQ=255)
                                                                         tatTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATc                          >  1:1442187/1‑100 (MQ=255)
                                                                          atTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCg                         <  2:2049982/100‑1 (MQ=255)
                                                                            nattcaggTCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaa                       >  2:2364931/8‑100 (MQ=25)
                                                                            cattcaggTCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaa                       >  1:2456197/8‑100 (MQ=25)
                                                                             tttttCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaa                      <  2:2425119/100‑1 (MQ=255)
                                                                              ttttCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaaa                     >  2:26639/1‑100 (MQ=255)
                                                                                 tCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATa                  <  2:2368909/100‑1 (MQ=255)
                                                                                  ggTCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCTGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc                 >  1:3574708/2‑100 (MQ=37)
                                                                                  ggTCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc                 >  2:1256916/2‑100 (MQ=38)
                                                                                   gTCTCGCGCACGTACGGTGCCCGTGACTTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                <  2:3764386/100‑1 (MQ=255)
                                                                                   gTCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                >  2:951715/1‑100 (MQ=38)
                                                                                   gTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                <  2:2530234/100‑1 (MQ=255)
                                                                                    tCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCt               >  1:786311/1‑100 (MQ=38)
                                                                                     cACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTg              >  2:128053/1‑100 (MQ=255)
                                                                                     cACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTg              >  2:969892/1‑100 (MQ=255)
                                                                                      aCGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGGGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGc             <  1:2152324/100‑1 (MQ=255)
                                                                                      aCGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGc             <  2:3836131/100‑1 (MQ=255)
                                                                                       cgcgCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGATATAACGAGTCCGCACGAGCCGGATCGAACAATAACTGCa            >  1:2184359/1‑100 (MQ=255)
                                                                                        gcgcACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCAc           <  1:199639/100‑1 (MQ=255)
                                                                                         ngcACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTCAACGCGTCCGCACGATCCGGATCGAAAAATACCTGccc           >  1:3022361/2‑97 (MQ=255)
                                                                                              cGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATAc      <  2:3696849/100‑1 (MQ=255)
                                                                                              cGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATAc      <  2:1632150/100‑1 (MQ=255)
                                                                                                tACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCt    >  1:3033599/1‑100 (MQ=255)
                                                                                                tACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCt    >  2:1365983/1‑100 (MQ=255)
                                                                                                tACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGGCCAGAGGCCAGCTTTAACCCGGCCGCACGACGCGGATCGAAAAATACCTGCCCGATACCt    >  1:3812803/1‑100 (MQ=255)
                                                                                                  cGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTc  >  1:985585/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
ACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTC  >  NC_000913/1950203‑1950399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: