Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,950,296:1 +C 15.8% coding (227/1773 nt) aspS ← aspartyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,950,2951.C15.8% 485.9 / 97.2 139coding (228/1773 nt)aspSaspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base . (60/57);  new base C (13/9);  total (73/66)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

Only 100 of 141 total aligned reads displayed.

CGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCAC  >  NC_000913/1950196‑1950390
                                                                                                    |                                                                                               
cGGCAGAACATCTGCGCGGGTAATGATAGGCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCa                                                                                                  <  1:2294356/100‑1 (MQ=255)
   cAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGt                                                                                              >  2:2152339/1‑100 (MQ=255)
    aGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTa                                                                                             >  2:2524999/1‑100 (MQ=255)
       aCATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACg                                                                                           <  2:3122829/100‑1 (MQ=255)
          tCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGc                                                                                       <  1:622506/100‑1 (MQ=255)
           cTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGcc                                                                                      <  1:2768388/100‑1 (MQ=255)
           cTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGcc                                                                                      >  2:2859947/1‑100 (MQ=255)
            tGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGccc                                                                                     <  1:406110/100‑1 (MQ=255)
            tGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGccc                                                                                     >  2:1676055/1‑100 (MQ=255)
                cgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGa                                                                                 <  1:1572083/100‑1 (MQ=38)
                 ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAc                                                                                <  1:227702/100‑1 (MQ=38)
                 ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                  <  1:2674960/98‑1 (MQ=255)
                 ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGAc                                                                                >  2:3540029/1‑100 (MQ=255)
                 ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGAc                                                                                <  2:3644544/100‑1 (MQ=255)
                 ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGAc                                                                                <  2:2944384/100‑1 (MQ=255)
                 ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGACCGTGa                                                                                 >  1:1332621/1‑99 (MQ=255)
                  gTTGATGATTGTCAGCGCGGACGCCAGCTCTTCGATTTCGCCGGTCGCCATATCGCGGTTGATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTgcc                                                                                <  1:2602981/99‑3 (MQ=255)
                  gTTGATGATAGTCAGCGAGGACGCCCGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAcg                                                                               <  2:645759/100‑2 (MQ=37)
                  gTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAcg                                                                               <  1:983416/100‑2 (MQ=38)
                  gTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGAcc                                                                               >  2:451763/1‑100 (MQ=255)
                    tgatgaTAGTCAGCGAGGACGCCAGCCCTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCTCGTGAcgaa                                                                             <  2:836261/100‑4 (MQ=37)
                    tgatgaTAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTg                                                                             <  2:2232549/100‑1 (MQ=255)
                       tgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTTTTTACTCTCA‑CGTACGGTGCCCGTGACCTGAAt                                                                          >  1:2707293/1‑100 (MQ=255)
                       tgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAcgaaaaa                                                                          <  2:3545802/100‑7 (MQ=25)
                       tgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAAt                                                                          >  1:2151737/1‑100 (MQ=255)
                        gatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATg                                                                         <  1:2864170/100‑1 (MQ=255)
                          tAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCa                                                                       <  2:2786555/100‑1 (MQ=255)
                          tAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTGCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCa                                                                       <  1:1199549/100‑1 (MQ=255)
                            gTCAGCGAGTACGCCAGCACTTCGTTTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCTTCACCCGCA‑CGTACGGTGCCCGTGTCCTGAATGCAGa                                                                     <  1:2434712/100‑1 (MQ=255)
                            gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGCTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGa                                                                     <  2:615211/100‑1 (MQ=255)
                             tCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGaa                                                                    <  2:1671023/100‑1 (MQ=255)
                              cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                   >  2:2958988/1‑100 (MQ=255)
                              cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                   <  1:1611557/100‑1 (MQ=255)
                              cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                   >  1:424946/1‑100 (MQ=255)
                              cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                   >  1:1264691/1‑100 (MQ=255)
                              cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                   <  2:1978231/100‑1 (MQ=255)
                               aGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACt                                                                  >  2:2634753/1‑100 (MQ=255)
                               aGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACt                                                                  >  1:1469474/1‑100 (MQ=255)
                                gCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTc                                                                 >  2:89845/1‑100 (MQ=255)
                                    ggACGCCAGCACTTCGATTTCGCCGGTCGCCCTATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTa                                                             >  1:913890/1‑100 (MQ=255)
                                    ggACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTa                                                             >  2:1061371/1‑100 (MQ=255)
                                         ccAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAg                                                        <  2:3593434/100‑1 (MQ=255)
                                         ccAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAg                                                        >  2:1430446/1‑100 (MQ=255)
                                           aGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGtt                                                      >  2:939987/1‑100 (MQ=255)
                                               cTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCaga                                                  >  2:1235072/1‑100 (MQ=255)
                                               cTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCaga                                                  >  2:556717/1‑100 (MQ=255)
                                                ttCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCagag                                                 <  1:3759021/100‑1 (MQ=255)
                                                ttCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTATAAGCAGAACTCATTACGCAGCTccaa                                                  >  1:2705242/1‑96 (MQ=255)
                                                 tCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAgg                                                >  1:1929744/1‑100 (MQ=255)
                                                 tCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAgg                                                >  1:1929759/1‑100 (MQ=255)
                                                 tCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAgg                                                >  2:3799814/1‑100 (MQ=255)
                                                 tCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGGCACGCGCA‑CGTACGGTGCCCGTAACCTGAATACACAACTCACTCCGCAGTACAGAgg                                                >  1:2253582/1‑100 (MQ=255)
                                                    aTTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTAGGCAGTTCAGAGGcc                                              >  2:3811663/1‑99 (MQ=255)
                                                    aTTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCa                                             >  1:3665881/1‑100 (MQ=255)
                                                     tttCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAg                                            <  2:3062018/100‑1 (MQ=255)
                                                        cGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCtt                                         <  2:1264699/100‑1 (MQ=255)
                                                        cGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCtt                                         >  2:2810014/1‑100 (MQ=255)
                                                        cGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCtt                                         >  1:2378886/1‑100 (MQ=255)
                                                        cGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCtt                                         >  2:3574017/1‑100 (MQ=255)
                                                        cGCCGGTCGCCATATCGCGGTGAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCtt                                         <  1:3366903/100‑1 (MQ=255)
                                                         gCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCttt                                        >  2:970118/1‑100 (MQ=255)
                                                          ccGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTa                                       <  2:3418716/100‑1 (MQ=255)
                                                          ccGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTa                                       <  2:131710/100‑1 (MQ=255)
                                                          ccGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACCGTGCCCGTGACCTGAATGCATAACTCATTACCCAGCTCCGAGCCCACCttt                                        >  1:3040107/1‑99 (MQ=255)
                                                           cGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTaa                                      <  1:1110149/100‑1 (MQ=255)
                                                           cGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTaa                                      <  1:1110263/100‑1 (MQ=255)
                                                            ggTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAc                                     <  1:733470/100‑1 (MQ=255)
                                                              tCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAcgc                                   >  2:2383120/1‑100 (MQ=255)
                                                               cGCCATATCGCGGTTAATATTTTTTTCTTCTTTCCCA‑CGTACGGTGCCCGTCACCTGACTGCAGAACTCCTTACGCAGTTCAGCGGCCAGCTTTAAccc                                   >  1:1537770/1‑97 (MQ=255)
                                                                 ccATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTc                                >  2:1269036/1‑100 (MQ=255)
                                                                     atCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCa                            >  2:2197672/1‑100 (MQ=255)
                                                                      tCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCAc                           <  2:3330697/100‑1 (MQ=255)
                                                                        gcgGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGa                         >  2:851064/1‑100 (MQ=255)
                                                                         cgGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGAt                        <  2:582330/100‑1 (MQ=255)
                                                                         cgGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGAt                        >  1:2934203/1‑100 (MQ=255)
                                                                           gTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATcc                      <  2:3248375/100‑1 (MQ=255)
                                                                                tatTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATc                 >  1:1442187/1‑100 (MQ=255)
                                                                                 atTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCg                <  2:2049982/100‑1 (MQ=255)
                                                                                   nattcaggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaa              >  2:2364931/8‑100 (MQ=25)
                                                                                    tttttCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaa             <  2:2425119/100‑1 (MQ=255)
                                                                                     ttttCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaaa            >  2:26639/1‑100 (MQ=255)
                                                                                     ttttCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaaa            <  2:48165/100‑1 (MQ=255)
                                                                                      tttCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaaaa           <  2:643264/100‑1 (MQ=255)
                                                                                         ggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCTGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc        >  1:3574708/2‑100 (MQ=37)
                                                                                         ggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc        >  2:2193040/2‑100 (MQ=38)
                                                                                         ggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc        >  2:1256916/2‑100 (MQ=38)
                                                                                          gTCTCGCGCA‑CGTACGGTGCCCGTGACTTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc       <  2:3764386/100‑1 (MQ=255)
                                                                                          gTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc       >  2:3623442/1‑100 (MQ=38)
                                                                                          gTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc       >  2:2514282/1‑100 (MQ=38)
                                                                                          gTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc       >  2:951715/1‑100 (MQ=38)
                                                                                          gTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc       >  2:35932/1‑100 (MQ=38)
                                                                                          gTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc       <  2:2530234/100‑1 (MQ=255)
                                                                                           tCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCt      >  1:786311/1‑100 (MQ=38)
                                                                                            cACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTg     >  2:969892/1‑100 (MQ=255)
                                                                                             aCGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGGGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGc    <  1:2152324/100‑1 (MQ=255)
                                                                                             aCGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATGGAAAAATACCTGc    >  1:3389285/1‑100 (MQ=255)
                                                                                              cgcgCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCa   >  2:134575/1‑100 (MQ=255)
                                                                                               gcgcA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCAc  <  1:199639/100‑1 (MQ=255)
                                                                                                ngcA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTCAACGCGTCCGCACGATCCGGATCGAAAAATACCTGccc  >  1:3022361/2‑97 (MQ=255)
                                                                                                 gcA‑CGTACGGTGCCCGTGCCCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTTCAc  <  1:2591600/98‑1 (MQ=255)
                                                                                                    |                                                                                               
CGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCAC  >  NC_000913/1950196‑1950390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑