BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,756,553	A→T	100%	K286* (AAA→TAA)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,553	0	A	T	100.0%	388.9 / ‑6.7	115	K286* (AAA→TAA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (0/1); major base T (51/62); minor base C (0/1); total (51/64)

Only 100 of 120 total aligned reads displayed.

GAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAG  >  NC_000913/1756457‑1756649

gaagaaGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCtaaa                                                                                               <  1:3245633/100‑1 (MQ=255)

gaagaaGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCtaaa                                                                                               <  2:1996500/100‑1 (MQ=255)

gaagaaGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCtaaa                                                                                               >  1:1371154/1‑100 (MQ=255)

 aagaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCtaaaa                                                                                              >  1:1346133/1‑100 (MQ=255)

  agaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAc                                                                                             <  2:3466694/100‑1 (MQ=255)

  agaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAc                                                                                             <  1:1916685/100‑1 (MQ=255)

   gaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAcc                                                                                            <  1:1240957/100‑1 (MQ=255)

   gaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAcc                                                                                            <  2:2314853/100‑1 (MQ=255)

       ttATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACg                                                                                        <  2:2220740/100‑1 (MQ=255)

        tATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGc                                                                                       <  1:843072/100‑1 (MQ=255)

         aTCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGcc                                                                                      <  2:274870/100‑1 (MQ=255)

          tCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGcc                                                                                      <  1:2247945/99‑1 (MQ=255)

           cTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCg                                                                                    <  2:1802303/100‑1 (MQ=255)

              cGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACt                                                                                 >  2:35833/1‑100 (MQ=255)

                cccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcg                                                                               <  2:2559220/100‑1 (MQ=255)

                cccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcg                                                                               <  1:2920289/100‑1 (MQ=255)

                  cAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcgcg                                                                             <  2:590756/100‑1 (MQ=255)

                   agaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCgcgc                                                                            <  1:232669/100‑1 (MQ=255)

                   agaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCgcgc                                                                            <  2:625811/100‑1 (MQ=255)

                   agaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCgcgc                                                                            >  2:124240/1‑100 (MQ=255)

                     aagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAg                                                                          <  2:3658591/100‑1 (MQ=255)

                       gatgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGaa                                                                        <  1:2159662/100‑1 (MQ=255)

                        atgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAg                                                                       >  2:1492381/1‑100 (MQ=255)

                        atgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAg                                                                       <  1:1062733/100‑1 (MQ=255)

                         tgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGc                                                                      >  2:2101950/1‑100 (MQ=255)

                         tgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGc                                                                      >  2:1640664/1‑100 (MQ=255)

                            tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                   <  2:210003/100‑1 (MQ=255)

                             gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGAACCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                                                  >  1:1497036/1‑100 (MQ=255)

                              atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                                                 >  1:633246/1‑100 (MQ=255)

                              atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                                                 >  2:1883118/1‑100 (MQ=255)

                               tCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa                                                                >  2:896802/1‑100 (MQ=255)

                               tCGAAAAATGTATCCGTGCACGTAAAGTCGGTATCACTGCGACCCCGATGCTGGATTCCATGATCTAACACACACGCCCGACTCGGGCAGAAGACGGTGa                                                                >  2:3878131/1‑100 (MQ=255)

                                cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                                                               <  1:3443373/100‑1 (MQ=255)

                                 gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg                                                              >  2:2957057/1‑100 (MQ=255)

                                 gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg                                                              <  2:589042/100‑1 (MQ=255)

                                 gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCAAGCTGCTGGATTCCATGATATCACACCCACCGCCGCCTCGCGCAGCACCCGGTAAc                                                               >  1:3008437/1‑99 (MQ=255)

                                    aaaTGTATCCGTGTACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGCCTCGCGCAGAAGCCGGTGACGTTg                                                           <  1:3122667/100‑1 (MQ=255)

                                     aaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATTTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGc                                                          <  1:2116588/100‑1 (MQ=255)

                                     aaTGTATCCGTGCACGT‑‑AGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCaa                                                        >  1:1996023/1‑100 (MQ=255)

                                           tCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGc                                                    <  1:3477591/100‑1 (MQ=255)

                                            ccGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGcc                                                   >  1:3274268/1‑100 (MQ=255)

                                             cGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCa                                                  >  2:2094502/1‑100 (MQ=255)

                                             cGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCa                                                  >  2:812540/1‑100 (MQ=255)

                                             cGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCACGACTCGCGCAGAAGCCGGGGACGTTGCAAACGcc                                                   >  1:79576/1‑99 (MQ=255)

                                              gTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCAt                                                 >  1:3679632/1‑100 (MQ=255)

                                                gCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATcc                                               >  2:2577086/1‑100 (MQ=255)

                                                 cACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCt                                              >  2:63346/1‑100 (MQ=255)

                                                  aCGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTc                                             <  2:1738829/100‑1 (MQ=255)

                                                     tAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGAc                                          <  2:791668/100‑1 (MQ=255)

                                                     tAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGAc                                          >  2:824007/1‑100 (MQ=255)

                                                     tAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGAc                                          >  2:2874316/1‑100 (MQ=255)

                                                      aaaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACg                                         <  2:43285/100‑1 (MQ=255)

                                                       aaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACgg                                        >  2:2679024/1‑100 (MQ=255)

                                                       aaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACgg                                        <  1:982786/100‑1 (MQ=255)

                                                           cGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACt                                    >  2:1620030/1‑100 (MQ=255)

                                                            gTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTg                                   <  1:539465/100‑1 (MQ=255)

                                                            gTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTg                                   >  2:1367406/1‑100 (MQ=255)

                                                             ttATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGa                                  >  1:3647929/1‑100 (MQ=255)

                                                              tATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGAc                                 >  2:1357759/1‑100 (MQ=255)

                                                               aTCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACg                                >  1:509479/1‑100 (MQ=255)

                                                                tCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGc                               <  2:828627/100‑1 (MQ=255)

                                                                tCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGc                               <  1:2885041/100‑1 (MQ=255)

                                                                tCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGc                               >  2:2854201/1‑100 (MQ=255)

                                                                  catGCGACACCGATGCTGGATTCCATGATCTAAAACCCACGCCCGAATAGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAg                             >  1:3702370/3‑100 (MQ=255)

                                                                  aCTGCGACCCAGATGCTGGATGCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAg                             <  1:3681532/100‑1 (MQ=255)

                                                                   cTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGt                            <  2:2348601/100‑1 (MQ=255)

                                                                    tGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTg                           >  1:3174701/1‑100 (MQ=255)

                                                                     gCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGa                          <  1:2088923/100‑1 (MQ=255)

                                                                      cGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGAt                         <  1:645961/100‑1 (MQ=255)

                                                                       gACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATg                        <  1:3014490/100‑1 (MQ=255)

                                                                         cccAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCt                      <  1:698737/100‑1 (MQ=255)

                                                                         cccAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCt                      >  2:2267146/1‑100 (MQ=255)

                                                                          ccAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCTTCGACGGTACTGACGCAGTGATGCTg                     <  2:2357873/100‑1 (MQ=255)

                                                                            aGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTc                   <  2:2026220/100‑1 (MQ=255)

                                                                            aGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGAAGAAGCCGATGACGTTGCAAACGACATCCTCGACGGTACTGACGCAGAGATGCTg                     >  1:3481227/1‑98 (MQ=255)

                                                                              aTGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTg                 >  2:2534564/1‑100 (MQ=255)

                                                                               tGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTgg                <  1:1515271/100‑1 (MQ=255)

                                                                               tGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTgg                >  2:2052365/1‑100 (MQ=255)

                                                                                gCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGt               <  2:633249/100‑1 (MQ=255)

                                                                                gCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGt               <  2:386046/100‑1 (MQ=255)

                                                                                 cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              <  2:1322589/100‑1 (MQ=255)

                                                                                 cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              >  1:2325002/1‑100 (MQ=255)

                                                                                 cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              <  1:3260901/100‑1 (MQ=255)

                                                                                  tGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGa             <  1:1117556/100‑1 (MQ=255)

                                                                                   ggATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTgg                <  1:797736/96‑1 (MQ=255)

                                                                                   ggATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGaa            >  1:3577272/1‑100 (MQ=255)

                                                                                    gATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAAt           <  2:2940291/100‑1 (MQ=255)

                                                                                    gATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAAt           <  1:1357751/100‑1 (MQ=255)

                                                                                    gATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAAt           >  2:3489181/1‑100 (MQ=255)

                                                                                     aTTCCATGATCTAAAACCCACGCCCGACTCGCGCAGCAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATc          <  2:2719783/100‑1 (MQ=255)

                                                                                      ttCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATcc         <  2:42551/100‑1 (MQ=255)

                                                                                       tCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGCCGTTCCAAACGCCATCCTCGACGGCACTGACGCACTGATGCTGTCTGGTTAATc          >  1:879268/1‑98 (MQ=255)

                                                                                       tCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCg        <  2:3805221/100‑1 (MQ=255)

                                                                                       tCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCg        <  1:1600335/100‑1 (MQ=255)

                                                                                         cATGATCTAAAACCCACGCCCGACTCGGGCAGAAGCAGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCa      >  1:1478125/1‑100 (MQ=255)

                                                                                         cATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCa      >  2:1066736/1‑100 (MQ=255)

                                                                                         cATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGATGACAGGTGGATcc         >  1:1899260/1‑97 (MQ=255)

                                                                                          aTGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaa     <  2:3350092/100‑1 (MQ=255)

                                                                                             aTCTAAATCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACGGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAg  <  1:1834386/100‑1 (MQ=255)

                                                                                             aTCCAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGGCGTTGCAAACGACATACTCGACGGTACTGGCGCAGTGAGGATGTCTAGTGAATCCGCAAAAg  <  1:2809397/100‑1 (MQ=255)

GAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAG  >  NC_000913/1756457‑1756649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`