Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,914,888 T→G 100% Q216P (CAG→CCG)  proQ ← RNA chaperone, putative ProP translation regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,914,8880TG97.1% 467.4 / 1.6 135Q216P (CAG→CCG) proQRNA chaperone, putative ProP translation regulator
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base G (69/61);  total (72/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 136 total aligned reads displayed.

GCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTG  >  NC_000913/1914791‑1914984
                                                                                                 |                                                                                                
gcgGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCggg                                                                                                <  2:1684959/100‑1 (MQ=255)
  ggTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGggac                                                                                              >  1:3051150/1‑100 (MQ=255)
   gTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGggacg                                                                                             <  2:74215/100‑1 (MQ=255)
    tAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGggacgc                                                                                            >  2:2025986/1‑100 (MQ=255)
      aGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGggacgcgg                                                                                          <  1:2844900/100‑1 (MQ=255)
       gCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGggacgcgga                                                                                         >  2:2314830/1‑100 (MQ=255)
         cTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGggacgcggacg                                                                                       >  2:1597674/1‑100 (MQ=255)
         cTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGggacgcggacg                                                                                       <  2:750371/100‑1 (MQ=255)
          tAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGggacgcggacgc                                                                                      >  2:803658/1‑100 (MQ=255)
          tAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGggacgcggacgc                                                                                      >  2:2069475/1‑100 (MQ=255)
          tAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGggacgcggacgc                                                                                      >  2:1457159/1‑100 (MQ=255)
          tAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGggacgcggacgc                                                                                      >  2:3832525/1‑100 (MQ=255)
           aaaaaaCATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCCGCGCGCACACTCGAAGACATACCCGAATTCAGCCGGAGGCGGGCGcc                                                                                     >  1:3807159/1‑100 (MQ=255)
             aaaaCATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGt                                                                                   >  2:3212945/1‑100 (MQ=255)
              aaaCATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTc                                                                                  <  1:383190/100‑1 (MQ=255)
               aaCATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCt                                                                                 <  2:3872244/100‑1 (MQ=255)
                   tGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTgg                                                                             <  2:147009/100‑1 (MQ=255)
                   tGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTgg                                                                             >  2:319251/1‑100 (MQ=255)
                    gTTCATGCCTGGCGCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAACCAAAGACATACCCGAATTCAGCTGGACGCGgccgcc                                                                                     >  1:3880796/1‑91 (MQ=255)
                     ttCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTg                                                                           <  2:3460611/100‑1 (MQ=255)
                       cATGCCTGGCCCGGCCTCCGTTTCAGAACACCATGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGAt                                                                         <  1:3764148/100‑1 (MQ=255)
                       cATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTg                                                                           >  1:3218512/1‑98 (MQ=255)
                        aTGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGAtt                                                                        <  1:1625333/100‑1 (MQ=255)
                        aTGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGAtt                                                                        <  1:2010459/100‑1 (MQ=255)
                        aTGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGAtn                                                                        <  1:3649110/100‑2 (MQ=255)
                         tGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGAttt                                                                       >  2:1858932/1‑100 (MQ=255)
                          gcctggcctgGCCTCCGTTTCAGAACACCAGGTGTTCTGCGGGCACAATCAAAGATATACCCGACTTCAGCGGGACGCGGACGCCGTCTTTGGTGAtt                                                                        <  1:2553786/98‑1 (MQ=255)
                           ccTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTcn                                                                     <  1:249309/100‑2 (MQ=255)
                             tGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTa                                                                    <  2:3741016/99‑1 (MQ=255)
                              ggCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAAt                                                                  >  2:2573047/1‑100 (MQ=255)
                              ggCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAAt                                                                  <  1:803652/100‑1 (MQ=255)
                               gCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATa                                                                 <  2:278293/100‑1 (MQ=255)
                                cccGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATAc                                                                >  1:2949430/1‑100 (MQ=255)
                                   ggCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGt                                                             >  1:3372147/1‑100 (MQ=255)
                                    attgtgcgcgcAGAACACCTGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTg                                                            >  2:481962/11‑100 (MQ=38)
                                     ccTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTgg                                                           <  2:1785933/100‑1 (MQ=255)
                                     ccTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTgg                                                           <  2:2025216/100‑1 (MQ=255)
                                     ccTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTgg                                                           <  2:3039802/100‑1 (MQ=255)
                                       tCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGca                                                         >  2:1525628/1‑100 (MQ=255)
                                        ccGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGcat                                                        >  1:154184/1‑100 (MQ=255)
                                        ccGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGcat                                                        <  2:3185961/100‑1 (MQ=255)
                                        ccGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGCGGcat                                                        <  1:2010964/100‑1 (MQ=255)
                                         cGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGcatc                                                       >  2:1800861/1‑100 (MQ=255)
                                             tCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGcatccatc                                                   <  2:3379695/100‑1 (MQ=255)
                                             tCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGcatccatc                                                   >  2:2317912/1‑100 (MQ=255)
                                             tCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGcatccatc                                                   >  2:2449905/1‑100 (MQ=255)
                                              cAGAACACCAGGTGTTCTGCGCGCCCAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATcg                                                  >  1:2928490/1‑100 (MQ=255)
                                              cAGAACACCAGGTGTTCTGCGCGCACAATCGAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATcg                                                  >  2:3472414/1‑100 (MQ=255)
                                              cAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATcg                                                  <  2:2971001/100‑1 (MQ=255)
                                              cAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATcg                                                  >  1:3245931/1‑100 (MQ=255)
                                                gAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCAGTCTTTGGTGATTTCTAATACGGTGGCATCCATcgc                                                 >  1:1279718/1‑99 (MQ=255)
                                                 aaCACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGt                                               >  1:2515682/1‑100 (MQ=255)
                                                  acacCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGtt                                              <  2:54530/100‑1 (MQ=255)
                                                  acacCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGtt                                              <  1:3669394/100‑1 (MQ=255)
                                                     ccAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTg                                           <  1:1856180/100‑1 (MQ=255)
                                                     ccAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTg                                           >  2:216059/1‑100 (MQ=255)
                                                      cAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGa                                          >  1:2186553/1‑100 (MQ=255)
                                                      cAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGa                                          <  1:2523560/100‑1 (MQ=255)
                                                       aGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGAc                                         <  2:2992575/100‑1 (MQ=255)
                                                       aGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGAc                                         <  2:723377/100‑1 (MQ=255)
                                                            ttCTGCGCGCACAATCACAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCt                                    <  1:2401967/100‑1 (MQ=255)
                                                             tCTGCGCGCACAATCAAAGACCTACCCGAATTCAGCGGGCCGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCtt                                   >  1:607799/1‑100 (MQ=255)
                                                             tCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCtt                                   >  2:1086559/1‑100 (MQ=255)
                                                             tCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCtt                                   <  1:3253842/100‑1 (MQ=255)
                                                              cTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCttt                                  >  2:386816/1‑100 (MQ=255)
                                                               tGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTc                                 <  2:2388271/100‑1 (MQ=255)
                                                               tGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTc                                 <  2:3826733/100‑1 (MQ=255)
                                                               tGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTc                                 <  2:2955034/100‑1 (MQ=255)
                                                                gcgcgcACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCa                                <  1:1456206/100‑1 (MQ=255)
                                                                  gcgcACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCAcc                              <  2:3350944/100‑1 (MQ=255)
                                                                   cgcACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCt                             >  2:956688/1‑100 (MQ=255)
                                                                    gcACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCtt                            >  2:2792692/1‑100 (MQ=255)
                                                                      acaATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCa                          >  1:1984716/1‑100 (MQ=255)
                                                                         aTCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAggg                       >  2:3093443/1‑100 (MQ=255)
                                                                          tCAACGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGc                      <  1:3825113/100‑1 (MQ=255)
                                                                           cAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCt                     <  2:1996313/100‑1 (MQ=255)
                                                                           cAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCt                     <  1:724707/100‑1 (MQ=255)
                                                                            aaaGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCtt                    >  2:2581694/1‑100 (MQ=255)
                                                                             aaGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTg                   >  2:2507074/1‑100 (MQ=255)
                                                                             aaGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTg                   <  2:3715133/100‑1 (MQ=255)
                                                                               gACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTc                 <  1:3854637/100‑1 (MQ=255)
                                                                                aCATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTcc                >  2:2586391/1‑100 (MQ=255)
                                                                                 cATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCg               <  1:2507056/100‑1 (MQ=255)
                                                                                  aTACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGa              <  1:1032074/100‑1 (MQ=255)
                                                                                  aTACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGa              <  1:3093419/100‑1 (MQ=255)
                                                                                   tACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGAc             >  2:1903374/1‑100 (MQ=255)
                                                                                     cccGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAg           >  2:3650984/1‑100 (MQ=255)
                                                                                     cccGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAg           >  2:1060477/1‑100 (MQ=255)
                                                                                     cccGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAg           >  2:350269/1‑100 (MQ=255)
                                                                                     cccGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAg           >  2:1791283/1‑100 (MQ=255)
                                                                                     cccGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAg           <  2:338752/100‑1 (MQ=255)
                                                                                     cccGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAg           >  2:451273/1‑100 (MQ=255)
                                                                                     cccGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAg           >  2:2665661/1‑100 (MQ=255)
                                                                                     cccGAATTCAGCGAGACGCGGACGCGGTCTTTGGTGATTTCTAATACAGTGGCGTCAATCGCGTTTTGACCCGCTTTCACCTTCAGGTCTTGTCCGACAg           >  2:3863497/1‑100 (MQ=255)
                                                                                         aaTTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCag       <  1:2247714/100‑1 (MQ=255)
                                                                                          aTTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCaga      <  1:1735734/100‑1 (MQ=255)
                                                                                            tCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGc    <  2:1722845/100‑1 (MQ=255)
                                                                                            tCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGc    <  1:1249699/100‑1 (MQ=255)
                                                                                             cAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCt   <  2:2071193/100‑1 (MQ=255)
                                                                                              aGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTg  >  1:1726298/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTG  >  NC_000913/1914791‑1914984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: