| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,955,062 | A→T | D314E (GAT→GAA) | recB ← | exonuclease V (RecBCD complex), beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,955,062 | 0 | A | T | 98.3% | 207.0 / NA | 60 | D314E (GAT→GAA) | recB | exonuclease V (RecBCD complex), beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (33/26); minor base C (0/1); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACGGCGGCGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGG > NC_000913/2954965‑2955157 | cACGGCGGCGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTgttc < 1:1038114/100‑1 (MQ=255) ggcggcGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTGgttcgat < 2:840341/100‑1 (MQ=255) gcgTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGcc < 2:142665/100‑1 (MQ=255) gcgTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGcc > 1:1303565/1‑100 (MQ=255) cgTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCt > 1:355089/1‑100 (MQ=255) ttCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAAc > 1:352755/1‑100 (MQ=255) ttCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAAc < 2:1174322/100‑1 (MQ=255) cgcgCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTg > 1:1425094/1‑100 (MQ=255) cgcgCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTg > 2:249/1‑100 (MQ=255) cgcTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGa > 2:413443/1‑100 (MQ=255) aCTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTc > 2:1383847/1‑100 (MQ=255) cTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTcg > 2:315711/1‑100 (MQ=255) tCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCgggg > 1:1329379/1‑100 (MQ=255) cgcgGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGTAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGt < 1:984886/100‑1 (MQ=255) cgcgGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGt > 2:509377/1‑100 (MQ=255) cgGATCTCAGCCAATGCGCGGGTGATCGCCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGAGGGGttt < 1:521508/100‑1 (MQ=255) ggATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTc < 2:497206/100‑1 (MQ=255) ggATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTc > 2:844394/1‑100 (MQ=255) gATCTCAGCCAATGCTCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTcc < 2:1578084/100‑1 (MQ=255) gATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTcc > 2:1047154/1‑100 (MQ=255) gATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTcc < 1:1383833/100‑1 (MQ=255) aTCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTccc > 2:1063021/1‑100 (MQ=255) tctcAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTcccc < 2:607466/100‑1 (MQ=255) tcAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTcccccc > 2:540479/1‑100 (MQ=255) cAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTccccccc > 1:221116/1‑100 (MQ=255) cAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTccccccc > 1:1154775/1‑100 (MQ=255) cAGCCAATGCGCGGGTGATCACCAGACCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTccccccc > 1:266133/1‑100 (MQ=255) aGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCg > 2:1147230/1‑100 (MQ=255) aGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCg < 2:1550957/100‑1 (MQ=255) cAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGAGGGGTTTCCCCCCCGGcc < 1:179685/100‑1 (MQ=255) tGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAATCAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTc < 2:222265/100‑1 (MQ=255) gcgcGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACCGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCg > 1:391184/1‑100 (MQ=255) cgcgGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGt > 1:180438/1‑100 (MQ=255) gcgGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTg > 2:482734/1‑100 (MQ=255) gggTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCg < 2:1372856/100‑1 (MQ=255) gggTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCg < 1:1145361/100‑1 (MQ=255) ggTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGa > 2:1223311/1‑100 (MQ=255) gTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGAt > 2:1456177/1‑100 (MQ=255) cACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGAtcttct > 1:669221/1‑100 (MQ=255) aCCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTa < 1:482730/100‑1 (MQ=255) aCCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTa < 2:409299/100‑1 (MQ=255) cAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAg < 2:727749/100‑1 (MQ=255) aTCGCGGATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGaaa > 2:49901/1‑100 (MQ=255) gATCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTgg > 2:1197410/1‑100 (MQ=255) aTCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTggg < 2:1010667/100‑1 (MQ=255) aTCGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTggg < 1:413439/100‑1 (MQ=255) cGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGgag > 1:1044034/1‑100 (MQ=255) cGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGgag < 1:844387/100‑1 (MQ=255) cGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGgag > 1:1543197/1‑100 (MQ=255) cGACAATGGTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGgag > 1:1238324/1‑100 (MQ=255) ggTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAAttttt < 2:1425108/100‑1 (MQ=255) ggTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAAttttt < 2:526644/100‑1 (MQ=255) gTTCTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTc < 2:894690/100‑1 (MQ=255) ttCTGCAAGCAGTTGCTCGATCGCCTCAAACAGTGGATGTAGCGGGGGTTCCCCCCCGGCCTTTGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTcc < 1:152867/100‑1 (MQ=255) tccTGCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTcc > 2:1082578/3‑100 (MQ=255) gCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCg < 2:1303579/100‑1 (MQ=255) gCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCg > 1:996391/1‑100 (MQ=255) gCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCg < 1:1545143/100‑1 (MQ=255) gCAAGCAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCg < 1:1063010/100‑1 (MQ=255) cAGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTcc < 2:1212974/100‑1 (MQ=255) aGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGCATTTTTCCCGCGACTCCg > 1:1300197/1‑100 (MQ=255) aGTTGTTCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCg > 2:499035/1‑100 (MQ=255) gttgttCGATCGCCTCAAACAGTGGAGGTCGCGGGGTTTCCCCCCCGCCCTTCGTGCGATCTTCGAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCgg < 1:1448006/100‑1 (MQ=255) | CACGGCGGCGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGG > NC_000913/2954965‑2955157 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |