Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	756,180	G→T	G92W (GGG→TGG)	sdhA →	succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	756,180	0	G	T	94.1%	222.5 / 1.9	68	G92W (GGG→TGG)	sdhA	succinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (36/28);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCG  >  NC_000913/756085‑756275                                                                                                |                                                                                                cccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGcc                                                                                             >  2:1380822/1‑100 (MQ=255)  ccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCg                                                                                            <  1:274834/100‑1 (MQ=255)  ccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCg                                                                                            >  1:645636/1‑100 (MQ=255)  ccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCg                                                                                            >  1:552801/1‑100 (MQ=255)   cATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCgg                                                                                           >  2:1559464/1‑100 (MQ=255)     tGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGaa                                                                                         >  2:70469/1‑100 (MQ=255)     tGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGaa                                                                                         >  2:1584335/1‑100 (MQ=255)         gATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATGGAATATATGTGTAAAACCTGGCCGGAAg                                                                                        >  2:1502735/1‑97 (MQ=255)           tAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGAtt                                                                                   >  2:37070/1‑100 (MQ=255)             aCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCt                                                                                 >  1:901253/1‑100 (MQ=255)              cTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTg                                                                                >  2:1415200/1‑100 (MQ=255)                gggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGa                                                                              <  1:51996/100‑1 (MQ=255)                gggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGa                                                                              <  2:1449151/100‑1 (MQ=255)                 ggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGaa                                                                             <  1:403889/100‑1 (MQ=255)                  gAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAAc                                                                            >  1:732965/1‑100 (MQ=255)                     tGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCg                                                                         <  2:1046484/100‑1 (MQ=255)                      ggCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGa                                                                        >  2:1588078/1‑100 (MQ=255)                       gCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGaa                                                                       <  2:999956/100‑1 (MQ=255)                        cATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAac                                                                      >  2:1001431/1‑100 (MQ=255)                        cATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAac                                                                      <  2:650201/100‑1 (MQ=255)                          tatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAacac                                                                    <  2:1442684/100‑1 (MQ=255)                          tatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAacac                                                                    <  2:637758/100‑1 (MQ=255)                           atGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAAcaca                                                                   >  2:1107375/1‑100 (MQ=255)                           atGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAAcaca                                                                   >  1:1361043/1‑100 (MQ=255)                            tGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACAt                                                                  >  2:1497508/1‑100 (MQ=255)                            tGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACAt                                                                  >  2:1522051/1‑100 (MQ=255)                               aCGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATggg                                                               <  2:1008429/100‑1 (MQ=255)                                 gACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGcc                                                             >  2:668016/1‑100 (MQ=255)                                  acacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCt                                                            >  1:615351/1‑100 (MQ=255)                                   cacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTg                                                           >  2:438186/1‑100 (MQ=255)                                     ccGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGcc                                                         >  1:393338/1‑100 (MQ=255)                                         gAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTtc                                                     <  2:672515/100‑1 (MQ=255)                                            aGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcg                                                  <  2:552805/100‑1 (MQ=255)                                            aGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcg                                                  <  1:801362/100‑1 (MQ=255)                                             gggTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcgc                                                 >  1:1057736/1‑100 (MQ=255)                                              ggTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcgcg                                                <  1:402491/100‑1 (MQ=255)                                               gTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGt                                               >  1:139401/1‑100 (MQ=255)                                               gTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGt                                               >  1:404417/1‑100 (MQ=255)                                                tCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtc                                              >  2:497582/1‑100 (MQ=255)                                                  ggACTATATCGGTGACCAGGACGCGATTGCATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGATCTCGAACACATGGGCCTGCCGTTCTCGCGtctc                                            <  1:958469/100‑1 (MQ=255)                                                  ggACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtctc                                            >  1:1514732/1‑100 (MQ=255)                                                     cTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgat                                         <  1:1395380/100‑1 (MQ=255)                                                         atCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgatgat                                      <  1:1415186/99‑1 (MQ=255)                                                             gTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCg                                 <  2:1007713/100‑1 (MQ=255)                                                               gACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGta                               <  1:1192930/100‑1 (MQ=255)                                                               gACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGta                               <  1:1330551/100‑1 (MQ=255)                                                                 ccAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatc                             >  1:1062045/1‑100 (MQ=255)                                                                  cAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatct                            >  1:1159146/1‑100 (MQ=255)                                                                    ggACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTAGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatcta                           <  1:539457/99‑1 (MQ=255)                                                                     gACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctatc                         <  2:811202/100‑1 (MQ=255)                                                                     gACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGATCGtatctatc                         <  1:895248/100‑1 (MQ=255)                                                                     gACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctatc                         <  1:1588063/100‑1 (MQ=255)                                                                      aCGCGATTGATTATATGTGTAAGACCGGGCCGGTAGTGATTCTGTAACTAAAACCCATGTGTCTGCCGTTCTCGCGTCTCGATGATGGTCGtatcta                           <  1:601566/97‑1 (MQ=255)                                                                      aCGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCa                        <  1:454317/100‑1 (MQ=255)                                                                       cgcgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCaa                       <  1:1001421/100‑1 (MQ=255)                                                                       cgcgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCaa                       <  2:108254/100‑1 (MQ=255)                                                                         cgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACg                     >  2:21960/1‑100 (MQ=255)                                                                         cgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACg                     <  2:740877/100‑1 (MQ=255)                                                                              gAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGt                >  2:366360/1‑100 (MQ=255)                                                                                 tatatGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTg             >  1:374026/1‑100 (MQ=255)                                                                                      gtgtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGt        >  1:619850/1‑100 (MQ=255)                                                                                       tgtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTc       <  2:901260/100‑1 (MQ=255)                                                                                        gtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCa      >  2:622097/1‑100 (MQ=255)                                                                                         tAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAg     >  1:1498147/1‑100 (MQ=255)                                                                                         tAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAg     >  1:1591848/1‑100 (MQ=255)                                                                                         tAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAg     >  1:951404/1‑100 (MQ=255)                                                                                          aaaaCCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGt    >  2:952256/1‑100 (MQ=255)                                                                                            aaCCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCg  >  1:564103/1‑100 (MQ=255)                                                                                                |                                                                                                CCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCG  >  NC_000913/756085‑756275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑