Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,553	A→T	K286* (AAA→TAA)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,553	0	A	T	97.0%	233.7 / ‑1.1	67	K286* (AAA→TAA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (32/33);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

AGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAG  >  NC_000913/1756459‑1756649                                                                                               |                                                                                                 agaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAc                                                                                             <  1:828785/100‑1 (MQ=255)    agTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCa                                                                                          >  2:208217/1‑100 (MQ=255)      ttATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCAcn                                                                                        <  2:439081/100‑2 (MQ=255)      ttATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACg                                                                                        <  2:1443231/100‑1 (MQ=255)      ttATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACg                                                                                        <  1:188723/100‑1 (MQ=255)            tCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACCTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGAc                                                                                  <  2:1046527/100‑1 (MQ=255)                 cAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcgcg                                                                             <  1:56437/100‑1 (MQ=255)                 cAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcgcg                                                                             <  1:1381179/100‑1 (MQ=255)                 cAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTcgcg                                                                             <  1:289501/100‑1 (MQ=255)                   gaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCa                                                                           <  2:609080/100‑1 (MQ=255)                   gaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGAAAAAAAAAAAAAAAACCCACGCCCGACTCGCGCa                                                                           <  1:115432/100‑1 (MQ=255)                     agaTGATGATCGAAAAATGTATCCGTTCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGa                                                                         <  1:460644/100‑1 (MQ=255)                        tgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGc                                                                      >  2:634682/1‑100 (MQ=255)                        ngatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGc                                                                      >  1:1231456/2‑100 (MQ=255)                           tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                   >  2:308122/1‑100 (MQ=255)                           tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                                   >  2:458291/1‑100 (MQ=255)                            gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                                                  >  2:1552192/1‑100 (MQ=255)                             atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                                                 >  1:514294/1‑100 (MQ=255)                             atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                                                 <  2:1130167/100‑1 (MQ=255)                             atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                                                 <  2:1130217/100‑1 (MQ=255)                              atcgaaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa                                                                >  1:218375/5‑100 (MQ=255)                               cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                                                               >  1:629485/1‑100 (MQ=255)                                gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAcn                                                              <  2:1231770/100‑2 (MQ=255)                                   aagtGTATCCGTGAACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                                           <  1:207277/97‑1 (MQ=255)                                   aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                                           <  2:507993/100‑1 (MQ=255)                                     gatcctCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCa                                                         <  2:313003/95‑1 (MQ=255)                                      tGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCaa                                                        <  2:58589/100‑1 (MQ=255)                                         aTCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACg                                                     >  1:1132247/1‑100 (MQ=255)                                          tCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCaagatc                                                    >  1:313000/1‑96 (MQ=255)                                           ccGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGTATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTTACGTTGCAAACGcc                                                   <  1:1261026/100‑1 (MQ=255)                                           ccGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGcc                                                   >  2:631799/1‑100 (MQ=255)                                            cGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCa                                                  >  1:373126/1‑100 (MQ=255)                                              tGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCCTc                                                >  1:1470931/1‑100 (MQ=255)                                                  cGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCg                                            >  2:1501898/1‑100 (MQ=255)                                                   gTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGa                                           >  2:550665/1‑100 (MQ=255)                                                    tAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGAc                                          <  2:498157/100‑1 (MQ=255)                                                     aaaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACg                                         >  1:761695/1‑100 (MQ=255)                                                     aaaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACg                                         >  1:582040/1‑100 (MQ=255)                                                      aaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACgg                                        <  2:896658/100‑1 (MQ=255)                                                            ttATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGa                                  >  1:565099/1‑100 (MQ=255)                                                            ttATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGa                                  <  1:1064594/100‑1 (MQ=255)                                                              aTCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACg                                <  2:148450/100‑1 (MQ=255)                                                              aTCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACg                                >  1:1503747/1‑100 (MQ=255)                                                              aTCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACg                                >  2:735101/1‑100 (MQ=255)                                                               tCACTGCGACCCAGATTCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGc                               >  1:1275041/1‑100 (MQ=255)                                                               tCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGc                               >  1:431326/1‑100 (MQ=255)                                                                cACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAGGCGGGTGACATTGCAAACGCCATCCTCGACGGTACTGACGCa                              >  1:1122245/1‑100 (MQ=255)                                                                  cTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCACACGCCATCCTCGACGGTACTGACGCAg                             >  2:1058093/1‑99 (MQ=255)                                                                  cTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAg                             >  1:1139056/1‑99 (MQ=255)                                                                  cTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGt                            <  2:917502/100‑1 (MQ=255)                                                                     cGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGAt                         <  1:1454240/100‑1 (MQ=255)                                                                       aCCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGc                       <  2:436296/100‑1 (MQ=255)                                                                       aCCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGc                       <  2:84189/100‑1 (MQ=255)                                                                           aGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTc                   <  2:629578/100‑1 (MQ=255)                                                                              tGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTgg                >  2:525897/1‑100 (MQ=255)                                                                              tGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTgg                >  2:998312/1‑100 (MQ=255)                                                                                cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCTTCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              <  1:735447/100‑1 (MQ=255)                                                                                cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              <  2:1010452/100‑1 (MQ=255)                                                                                cTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg              <  1:1574806/100‑1 (MQ=255)                                                                                  ggATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTTACGTTGCAAACGCCATCCTCGACTGTACTGACGCAGTGATGCTGCCTGTTGaa            >  1:797199/1‑100 (MQ=255)                                                                                  ggATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGaa            >  2:1512999/1‑100 (MQ=255)                                                                                      tCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCg        <  2:678367/100‑1 (MQ=255)                                                                                       ccATGATCTAAAGCCCGCGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGc       <  2:1209054/100‑1 (MQ=255)                                                                                        cATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCa      >  2:359529/1‑100 (MQ=255)                                                                                        cATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCa      >  2:375601/1‑100 (MQ=255)                                                                                           gATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaaaa   <  2:77616/100‑1 (MQ=255)                                                                                            aTCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAg  >  2:413640/1‑100 (MQ=255)                                                                                               |                                                                                                 AGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAG  >  NC_000913/1756459‑1756649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑