Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,950,296:1 +C 15.3% coding (227/1773 nt) aspS ← aspartyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,950,2951.C15.3% 502.8 / 94.6 144coding (228/1773 nt)aspSaspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base . (54/68);  new base C (9/13);  total (63/81)
Fisher's exact test for biased strand distribution p-value = 8.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

Only 100 of 147 total aligned reads displayed.

GGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATA  >  NC_000913/1950197‑1950394
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ggCAGAACATCCGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑c                                                                                                    <  2:2193346/100‑1 (MQ=255)
  cAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGt                                                                                                  >  2:3740856/1‑100 (MQ=255)
         tCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGc                                                                                           >  1:2156356/1‑100 (MQ=255)
           tGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGccc                                                                                         >  2:3668676/1‑100 (MQ=255)
            gcgcGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCg                                                                                        >  2:3331506/1‑100 (MQ=255)
            gcgcGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCg                                                                                        <  2:38662/100‑1 (MQ=255)
            gcgcGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCg                                                                                        <  2:38619/100‑1 (MQ=255)
              gcgGTTGATGATAGTCCGCGAGGGCGCCAGAACTTCGATTTCGCCGGTCGCCCTATCGCGGTTAACATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCg                                                                                        <  1:3018778/98‑1 (MQ=255)
              gcgGTTGATGATAGTCAGCGAGGACGCCAGCAGTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                      <  2:3743580/100‑1 (MQ=255)
              gcgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                      >  2:1444977/1‑100 (MQ=255)
              gcgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                      <  1:815167/100‑1 (MQ=255)
               cgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGa                                                                                     <  2:1891247/100‑1 (MQ=38)
               cgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGa                                                                                     <  1:1845594/100‑1 (MQ=255)
               cgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGa                                                                                     <  2:2356171/100‑1 (MQ=255)
                ggTTGATGATAGTCAGCGAGGACGCGAGCTCTTCGATTTGGCCGGTCGCCATATCGCGGTTACTATTTTGGTCGTCACGGGCACCGTACgtgcgagtgac                                                                                     <  1:1653246/100‑11 (MQ=255)
                ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAc                                                                                    <  2:3343654/100‑1 (MQ=38)
                ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAc                                                                                    <  2:3045737/100‑1 (MQ=38)
                ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAc                                                                                    <  1:3563502/100‑1 (MQ=38)
                ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGAc                                                                                    >  2:553780/1‑100 (MQ=255)
                ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGAc                                                                                    <  1:2815395/100‑1 (MQ=255)
                 gTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGAcc                                                                                   >  1:2182763/1‑100 (MQ=255)
                  ttGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAcga                                                                                  <  2:101669/100‑3 (MQ=37)
                   tgatgcTAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTg                                                                                 <  1:712976/100‑1 (MQ=255)
                   tgatgaTAGTCAGCGAGGACGCCAGCTCTTCTATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTg                                                                                 <  1:189879/100‑1 (MQ=255)
                     atgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAcgaaaa                                                                               <  2:1025372/100‑6 (MQ=25)
                     aggatAGTCAGCGGGGACGCCAGCACTTCGAATTCGCCGGTCACCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGaa                                                                               >  2:2571215/3‑100 (MQ=255)
                      tgatAGTCGGCGAGGGCGCCAGCACTTCGATTTCGGCGGTCGCCATATCGCGGTTAAGATTTGTTTAGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAAt                                                                              <  1:908795/100‑1 (MQ=255)
                       gatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAcgaaaaaa                                                                             <  2:2963636/100‑8 (MQ=25)
                       gatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATg                                                                             <  2:2375739/100‑1 (MQ=255)
                        atAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGc                                                                            >  1:1936426/1‑100 (MQ=255)
                        atAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGc                                                                            <  1:2970790/100‑1 (MQ=255)
                         tcttCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCa                                                                           <  2:2187008/97‑1 (MQ=255)
                           gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGa                                                                         <  1:508183/100‑1 (MQ=255)
                            tCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGaa                                                                        >  1:306608/1‑100 (MQ=255)
                             cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                       <  2:1213758/100‑1 (MQ=255)
                              aGCGAGGCCGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTCAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACt                                                                      <  2:2274328/100‑1 (MQ=255)
                              aGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACt                                                                      >  2:2736556/1‑100 (MQ=255)
                                cGAGGACGCGAGCACTACGATTTAGCCGGTCGCCATATCGCGGTTACTAGTTTTTTCGTCACGCACG‑CGTACGGTGCCCGTGACTTGAAt                                                                              <  1:2429950/90‑1 (MQ=255)
                                cGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCa                                                                    <  2:1165447/100‑1 (MQ=255)
                                 gAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCAt                                                                   >  2:3574931/1‑100 (MQ=255)
                                 gAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGACACGCGCA‑CGTACGGTGCCCGTGACCTGGATGCAGAACTctt                                                                   <  2:2574566/100‑3 (MQ=255)
                                  aGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCAtt                                                                  <  1:553774/100‑1 (MQ=255)
                                   ggACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTa                                                                 >  2:3526701/1‑100 (MQ=255)
                                     aCGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACg                                                               >  1:3251039/1‑100 (MQ=255)
                                        ccAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAg                                                            <  2:902932/100‑1 (MQ=255)
                                        ccAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAg                                                            >  1:2198939/1‑100 (MQ=255)
                                           gCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTc                                                         <  1:1372410/100‑1 (MQ=255)
                                              cttcgccgtCGCCCGTCGCCATACTCCGGTTACTATTTTTTTCGTCACGCGGC‑CGTACGGTGCCCGTGACCTCAATGCAGAACTCATTACGCAGTCCaca                                                      <  1:2276549/92‑3 (MQ=255)
                                              cTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTTTTTTTTTTTA‑CATACGGTGCCCGTGACCTGAATGCACAACTCATTACGCAGTTCa                                                        >  1:1731022/1‑98 (MQ=255)
                                              cTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCaga                                                      >  1:424428/1‑100 (MQ=255)
                                               ttCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCagag                                                     <  1:678617/100‑1 (MQ=255)
                                                tCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAgg                                                    <  2:2667489/100‑1 (MQ=255)
                                                tCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAgg                                                    >  2:1699947/1‑100 (MQ=255)
                                                tCGATTTCACCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAgg                                                    <  2:690196/100‑1 (MQ=255)
                                                 cGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGc                                                   >  2:1813498/1‑100 (MQ=255)
                                                  caTTCCGCCTGTCGCAATATCGCGCTTCACAGTTCCTTCTCCCCGCGCC‑TTTACAGTTCCCGTGAACTGAATTCAGAACTCATTACGCAGTTCagacg                                                    <  1:3485492/97‑3 (MQ=255)
                                                   aTTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCa                                                 >  2:3356495/1‑100 (MQ=255)
                                                   aTTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCa                                                 >  2:3650442/1‑100 (MQ=255)
                                                       cGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCtt                                             <  2:2075490/100‑1 (MQ=255)
                                                        gCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCttt                                            <  1:2858998/100‑1 (MQ=255)
                                                        gCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCttt                                            >  2:2533345/1‑100 (MQ=255)
                                                           ggTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAc                                         <  2:1342093/100‑1 (MQ=255)
                                                           ggTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCAGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTaa                                          <  1:608497/99‑1 (MQ=255)
                                                                 cATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTcc                                   >  2:3574164/1‑100 (MQ=255)
                                                                 cATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTcc                                   >  1:1895510/1‑100 (MQ=255)
                                                                    atCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGTTCTGAATGCATAACTCATTACGCAGTTCGGAGGCCAGCTTTAACGCGTCCGCa                                >  1:2681821/1‑100 (MQ=255)
                                                                     tCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCAc                               >  1:3720289/1‑100 (MQ=255)
                                                                     tCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCAc                               <  1:743001/100‑1 (MQ=255)
                                                                      cgcgGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACGCATTAAGCAGTTCACAGGCCAGCTTTAACGCGTCAGCACg                              >  1:2881542/1‑100 (MQ=255)
                                                                      cgaagatAATATATTTTTCGTAACGCGCC‑CGTACGGTGAAAGTGACCTGAATGAAAAACTCATTAAGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACg                              >  1:1880154/7‑100 (MQ=255)
                                                                         ggTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATc                           >  2:2050190/1‑100 (MQ=255)
                                                                           ttAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCg                         <  2:535987/100‑1 (MQ=255)
                                                                             aaTATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCgg                        >  2:1788662/1‑99 (MQ=255)
                                                                             aaTATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCgg                        >  1:3019122/1‑99 (MQ=255)
                                                                              atatTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGAt                      <  1:3572309/100‑1 (MQ=255)
                                                                              atatTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGAt                      >  2:2887612/1‑100 (MQ=255)
                                                                               tatTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATc                     <  1:1170290/100‑1 (MQ=255)
                                                                                atTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCg                    <  1:1598952/100‑1 (MQ=255)
                                                                                atTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCg                    >  1:947271/1‑100 (MQ=255)
                                                                                atTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCg                    <  1:3655769/100‑1 (MQ=255)
                                                                                atTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCg                    <  2:1855482/100‑1 (MQ=255)
                                                                                 ttgttttCGTCACGCGCA‑CGTACGGTGCCCGGGACCTGGTTGCAGAACGCAGTACGCAGCTCAGAGGCCAGCTTTAACGCGTCCGCACGAGCCGGAt                      <  1:1037275/94‑1 (MQ=255)
                                                                                   tttttCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGACTGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaa                 <  1:2398428/100‑1 (MQ=255)
                                                                                      caggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATAGAAAAAt              >  1:926400/4‑100 (MQ=37)
                                                                                      caggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGAATCGAAAAAt              >  2:285470/4‑100 (MQ=37)
                                                                                       tggTCACGCGCA‑CGTACGGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAGGCGTCCGCACGATCCGGTGCGAAGAAt              >  1:1642009/3‑99 (MQ=255)
                                                                                        ggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc            >  2:2520156/2‑100 (MQ=38)
                                                                                        ggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc            >  1:3538167/2‑100 (MQ=38)
                                                                                        ggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc            >  2:1249346/2‑100 (MQ=38)
                                                                                         gTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc           >  2:1557798/1‑100 (MQ=38)
                                                                                         gTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc           >  2:2017469/1‑100 (MQ=255)
                                                                                         gTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc           >  2:2332594/1‑100 (MQ=255)
                                                                                         gTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc           <  2:941600/100‑1 (MQ=255)
                                                                                           cACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTg         >  1:1029999/1‑100 (MQ=255)
                                                                                           cACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTg         <  1:35119/100‑1 (MQ=255)
                                                                                            aCGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGc        >  2:1536967/1‑100 (MQ=255)
                                                                                            aCGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGc        <  2:2266387/100‑1 (MQ=255)
                                                                                             cgcgCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCa       <  2:3428513/100‑1 (MQ=255)
                                                                                                 cA‑CCTACGGTGCCCTTCACCTGTATGCAGAACTCATTACGCATTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCa       <  1:3814431/96‑1 (MQ=255)
                                                                                                  a‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATa  <  2:937536/100‑1 (MQ=255)
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GGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATA  >  NC_000913/1950197‑1950394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑