BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913	3,815,859	Δ82 bp	100%		[rph]–[rph]	[rph], [rph]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3815859	3815940	82	159 [0]	[0] 159	[rph]–[rph]	[rph], [rph]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3815858	0 (0.000)	127 (1.060)	72/158	NT	100%	pseudogene (24/48 nt)	rph	ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
*	?	NC_000913	3815941 =	0 (0.000)	127 (1.060)	72/158	NT	100%	pseudogene (609/669 nt)	rph	ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH

Only 100 of 159 total aligned reads displayed.

ATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCA  >  NC_000913/3815759‑3815956

atGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGC                                                                                                    <  2:2010667‑M1/100‑1 (MQ=255)

  ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcg                                                                                                  <  1:3251820‑M1/100‑3 (MQ=255)

     ttCATGCCTTCTCTCCTGATCTTACTTTTAAAAAAAAAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcc                                                                                               <  1:2089879‑M1/100‑6 (MQ=255)

     ttCATGCCTTCGCTCTTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcc                                                                                               <  2:3756330‑M1/100‑6 (MQ=255)

     ttCATGCCTTCGCTCCTCATCTTATTTTACAAAAAAAAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcc                                                                                               <  1:3595359‑M1/100‑6 (MQ=255)

     ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcc                                                                                               >  2:1504416‑M1/1‑95 (MQ=255)

       cATGCCTTCGCTCCTCATCTTACTTTTCTACCGACAAAAAAAAGGCGACTCAGCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccc                                                                                             <  2:1774259‑M1/100‑8 (MQ=255)

       cATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccc                                                                                             >  1:451685‑M1/1‑93 (MQ=255)

        aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccct                                                                                            <  1:1402423‑M1/100‑9 (MQ=255)

          gCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAACAAGGCGACTCATCATTCGCCTTAAAAATCAATTTACCAGCGCCGCCTTCTGCcgtcccctgc                                                                                          >  1:923903‑M1/1‑90 (MQ=255)

           ccTTCGCTCCCCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgca                                                                                         >  2:487767‑M1/1‑89 (MQ=255)

            cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcac                                                                                        <  2:110975‑M1/100‑13 (MQ=255)

                gCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttca                                                                                    >  2:2411115‑M1/1‑84 (MQ=255)

                gCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttca                                                                                    >  2:2377688‑M1/1‑84 (MQ=255)

                 ctcctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaa                                                                                   <  2:2657372‑M1/100‑18 (MQ=255)

                  tcctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaat                                                                                  <  1:428503‑M1/100‑19 (MQ=255)

                   cctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatg                                                                                 <  1:359591‑M1/100‑20 (MQ=255)

                    ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatga                                                                                <  1:953264‑M1/100‑21 (MQ=255)

                     tcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgat                                                                               <  2:2958684‑M1/100‑22 (MQ=255)

                       aTCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgc                                                                             >  1:3792879‑M1/1‑77 (MQ=255)

                             cTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgt                                                                       >  1:211098‑M1/1‑71 (MQ=255)

                             cTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgt                                                                       >  1:2068433‑M1/1‑71 (MQ=255)

                               tttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtct                                                                     >  1:1654296‑M1/1‑69 (MQ=255)

                               tttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtct                                                                     >  2:3141868‑M1/1‑69 (MQ=255)

                               tttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtct                                                                     >  2:432832‑M1/1‑69 (MQ=255)

                               tatctACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgt                                                                       >  2:2584128‑M1/3‑69 (MQ=255)

                                ttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtctt                                                                    <  1:3800624‑M1/100‑33 (MQ=255)

                                  cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcg                                                                  >  2:192125‑M1/1‑66 (MQ=255)

                                  cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGACAGCACCGCCTTCTGCcgtccccggcgcttcaatgatgcgcccgtcttcg                                                                  >  1:1026876‑M1/1‑66 (MQ=255)

                                   tACAGACAGAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcgg                                                                 >  1:2383736‑M1/1‑65 (MQ=255)

                                   tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcgg                                                                 <  1:846778‑M1/100‑36 (MQ=255)

                                   tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcgg                                                                 <  1:1241610‑M1/100‑36 (MQ=255)

                                   cacAGACAAAAAAAAGGCGACTCCTCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcgg                                                                 <  1:3707890‑M1/99‑36 (MQ=255)

                                      aGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtca                                                              >  2:3706066‑M1/1‑62 (MQ=255)

                                       gACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcat                                                             >  2:2780242‑M1/1‑61 (MQ=255)

                                       gACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcat                                                             >  1:3649610‑M1/1‑61 (MQ=255)

                                        aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatc                                                            <  1:1868591‑M1/100‑41 (MQ=255)

                                         gatttttaaggcgactGATGAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatca                                                           >  2:753794‑M1/8‑59 (MQ=255)

                                         gatttttaaggcgactGATGAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatca                                                           >  2:2536528‑M1/8‑59 (MQ=255)

                                         cAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatca                                                           >  1:2994313‑M1/1‑59 (MQ=255)

                                         cAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatca                                                           <  1:1007777‑M1/100‑42 (MQ=255)

                                          aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcac                                                          >  2:3627779‑M1/1‑58 (MQ=255)

                                           tttttaaggcgaCTGATGAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcact                                                         >  2:2494398‑M1/6‑57 (MQ=255)

                                            ttttaaggcgACTGATGAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcacta                                                        >  2:1552870‑M1/5‑56 (MQ=255)

                                            aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcacta                                                        <  1:129655‑M1/100‑45 (MQ=255)

                                            aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGACGCCTTCTGCagtccactgcacttcaatgctgcgcacgtcttcggtcatcac                                                          >  1:1892909‑M1/1‑56 (MQ=255)

                                             tttaaggcGACTGATGAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactcc                                                       >  2:1677515‑M1/4‑55 (MQ=255)

                                              ttaaggCGACTGATGAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacg                                                      >  2:789145‑M1/3‑54 (MQ=255)

                                              aaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacg                                                      <  1:2648291‑M1/100‑47 (MQ=255)

                                              aaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacg                                                      >  2:45951‑M1/1‑54 (MQ=255)

                                               taagGCGACTGATGAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgt                                                     >  2:1148053‑M1/2‑53 (MQ=255)

                                               taagGCGACTGATGAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcgctcatcactacg                                                      >  2:2808277‑M1/2‑53 (MQ=255)

                                                  ggCGACTTATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttca                                                  >  2:3441879‑M1/1‑50 (MQ=255)

                                                  ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttca                                                  <  1:3089458‑M1/100‑51 (MQ=255)

                                                    cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttgaatgatgcgcccgtcttcggtcatcactacgttcatg                                                <  1:2039494‑M1/100‑53 (MQ=255)

                                                    cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatg                                                >  1:566932‑M1/1‑48 (MQ=255)

                                                      aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtc                                              >  1:1832820‑M1/1‑46 (MQ=255)

                                                       cTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcg                                             >  2:1596726‑M1/1‑45 (MQ=255)

                                                        tcatcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcgg                                            <  1:2521546‑M1/100‑57 (MQ=255)

                                                        tcatcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcgg                                            <  2:137719‑M1/100‑57 (MQ=255)

                                                        tcatcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcgg                                            <  2:2947643‑M1/100‑57 (MQ=255)

                                                         catcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggt                                           >  2:2481049‑M1/1‑43 (MQ=255)

                                                          atcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtc                                          <  1:1532500‑M1/100‑59 (MQ=255)

                                                            caGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctc                                        <  2:1166136‑M1/100‑61 (MQ=255)

                                                             aGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctct                                       >  2:2086248‑M1/1‑39 (MQ=255)

                                                              gTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctg                                      >  2:2249223‑M1/1‑38 (MQ=255)

                                                                cGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcg                                    <  2:718435‑M1/100‑65 (MQ=255)

                                                                cGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcg                                    <  1:2780228‑M1/100‑65 (MQ=255)

                                                                  ccTTAAAAATCAGTTTGCCCGCGCCGCCTTCTGCcgtcccctgcatttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggc                                  <  1:3375536‑M1/100‑67 (MQ=255)

                                                                  ccTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggc                                  <  1:3441859‑M1/100‑67 (MQ=255)

                                                                  ccTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggc                                  >  1:3361734‑M1/1‑34 (MQ=255)

                                                                   cTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggca                                 >  2:2185687‑M1/1‑33 (MQ=255)

                                                                   cTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcgaca                                 >  2:2851109‑M1/1‑33 (MQ=255)

                                                                    ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcag                                <  1:675921‑M1/100‑69 (MQ=255)

                                                                     tAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcaga                               >  2:3242972‑M1/1‑31 (MQ=255)

                                                                        aaaTCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtc                            <  1:735923‑M1/100‑73 (MQ=255)

                                                                          aTCAGTTTGCCAGCGCCGCCGTCTGCcgtcccctgcacttcaatgatgcgcccgacttcggtcatcactacgtgcatgtcggtctctgcggcagagtct                           <  1:662580‑M1/99‑74 (MQ=255)

                                                                           tCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttc                         <  2:2168785‑M1/100‑76 (MQ=255)

                                                                           tCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttc                         <  1:3706043‑M1/100‑76 (MQ=255)

                                                                            cAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttca                        <  1:756233‑M1/100‑77 (MQ=255)

                                                                            cAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttca                        <  2:621457‑M1/100‑77 (MQ=255)

                                                                              gTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaa                       >  1:1348633‑M1/1‑22 (MQ=255)

                                                                               tttGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacg                     >  2:1289951‑M1/1‑21 (MQ=255)

                                                                               tttGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacg                     >  2:3638748‑M1/1‑21 (MQ=255)

                                                                                 tGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgta                   <  1:2705667‑M1/100‑82 (MQ=255)

                                                                                     aGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattcc               <  2:776655‑M1/100‑86 (MQ=255)

                                                                                     aGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattcc               <  1:292325‑M1/100‑86 (MQ=255)

                                                                                      gcgcCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattcca              >  1:1668684‑M1/1‑14 (MQ=255)

                                                                                        gccgccTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccaga            >  2:1006236‑M1/1‑12 (MQ=255)

                                                                                          cgccTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatc          <  1:3485173‑M1/100‑91 (MQ=255)

                                                                                          cgccTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatc          <  1:1926714‑M1/100‑91 (MQ=255)

                                                                                          cgccTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatc          >  2:3502466‑M1/1‑10 (MQ=255)

                                                                                          cgccTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatc          >  2:3004233‑M1/1‑10 (MQ=255)

                                                                                           gccTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcg         <  1:2481037‑M1/100‑92 (MQ=255)

                                                                                            nctTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggttatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgc        >  2:1612217‑M1/2‑8 (MQ=255)

                                                                                             cTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgca       >  2:1529904‑M1/1‑7 (MQ=255)

                                                                                                 tGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaacc   >  2:1032541‑M1/1‑3 (MQ=255)

                                                                                                 tGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaacc   <  2:705702‑M1/100‑98 (MQ=255)

                                                                                                 tGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaacc   <  2:3090964‑M1/100‑98 (MQ=255)

                                                                                                  gCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaaccg  >  2:3374900‑M1/1‑2 (MQ=255)

ATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCA  >  NC_000913/3815759‑3815956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`