BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913	1,978,503	Δ776 bp	100%		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1978503–1979270	1979278	9–776	173 [0]	[1] 172	insB1–insA	insB1, insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1978502	0 (0.000)	148 (1.200)	77/162	NT	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
*	?	NC_000913	1979279 =	0 (0.000)	148 (1.200)	77/162	NT	100%	intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

Only 100 of 172 total aligned reads displayed.

GAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATG  >  NC_000913/1979181‑1979377

gtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgta                                                                                                   <  2:3367885‑M2/2‑1 (MQ=255)

 tactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgtat                                                                                                  >  1:3783219‑M2/98‑100 (MQ=255)

     cgtcactacacgcacatacaacggaggggggctgcgatttttaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAg                                                                                               >  1:1712770‑M2/94‑99 (MQ=255)

     cgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGt                                                                                              >  2:361370‑M2/94‑100 (MQ=255)

      gtcactacacgcacatacaacggaggggggctgagattttcaataatgcgtgatgcagatcacacaaaacgctcaattacttaacataaatgTATAAGTc                                                                                             >  1:2298871‑M2/93‑100 (MQ=255)

        cactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCAt                                                                                           <  1:1002680‑M2/10‑1 (MQ=255)

         actacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATa                                                                                          >  2:2855473‑M2/90‑100 (MQ=255)

         actacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATa                                                                                          <  1:3149343‑M2/11‑1 (MQ=255)

           tacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaattTATAAGTCataat                                                                                        <  2:2567463‑M2/13‑3 (MQ=255)

             cacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttt                                                                                      <  2:1894805‑M2/15‑1 (MQ=255)

               cgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttg                                                                                    <  2:3683798‑M2/17‑1 (MQ=255)

               cgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttg                                                                                    <  1:3095763‑M2/17‑1 (MQ=255)

               cgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttg                                                                                    <  2:1492887‑M2/17‑1 (MQ=255)

                 cacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgtt                                                                                  <  1:1981199‑M2/19‑1 (MQ=255)

                   catacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgtttt                                                                                <  1:1778219‑M2/21‑1 (MQ=255)

                   catacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgtttt                                                                                >  2:2430029‑M2/80‑100 (MQ=255)

                    atacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgttttg                                                                               <  2:1146768‑M2/22‑1 (MQ=255)

                    atacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgttttg                                                                               <  2:446981‑M2/22‑1 (MQ=255)

                     tacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                              >  2:2388355‑M2/78‑100 (MQ=255)

                      acaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                             <  2:3576029‑M2/24‑1 (MQ=255)

                      acaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                             <  1:2407350‑M2/24‑1 (MQ=255)

                      acaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                             <  1:2092136‑M2/24‑1 (MQ=255)

                      acaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                             <  2:3447509‑M2/24‑1 (MQ=255)

                      acaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                             <  1:289692‑M2/24‑1 (MQ=255)

                      acaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                             <  1:2695519‑M2/24‑1 (MQ=255)

                       caacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgt                                                                            >  1:801651‑M2/76‑100 (MQ=255)

                       caacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgt                                                                            >  2:558816‑M2/76‑100 (MQ=255)

                       caacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACGTTTGTTTTGGgt                                                                            <  2:2511828‑M2/25‑1 (MQ=255)

                        aacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgtg                                                                           >  2:3776876‑M2/75‑100 (MQ=255)

                             aggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTAttt                                                                      <  2:2954532‑M2/31‑1 (MQ=255)

                              gggtggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTc                                                                     <  1:541453‑M2/32‑1 (MQ=255)

                              ggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTc                                                                     <  1:2855459‑M2/32‑1 (MQ=255)

                              ggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTc                                                                     <  1:2125623‑M2/32‑1 (MQ=255)

                              ggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTATATTTc                                                                     <  2:2145957‑M2/32‑1 (MQ=255)

                                ggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCaa                                                                   <  1:1620483‑M2/34‑1 (MQ=255)

                                  ggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCaa                                                                   <  1:1075514‑M2/34‑1 (MQ=255)

                                  ggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATc                                                                 <  2:1113914‑M2/36‑1 (MQ=255)

                                   gctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCaa                                                                   <  1:1180048‑M2/34‑1 (MQ=255)

                                     tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGt                                                              >  2:104914‑M2/62‑100 (MQ=255)

                                     tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGt                                                              >  2:2649642‑M2/62‑100 (MQ=255)

                                     tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTATTGGGTGTATTTCAATCTGt                                                              >  1:940250‑M2/62‑100 (MQ=255)

                                       cgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTa                                                            <  1:2941462‑M2/41‑1 (MQ=255)

                                       cgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTa                                                            >  1:1166895‑M2/60‑100 (MQ=255)

                                       cgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTa                                                            >  1:1241905‑M2/60‑100 (MQ=255)

                                       cgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTa                                                            >  1:619228‑M2/60‑100 (MQ=255)

                                       atcttttcaataatgcatgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTa                                                            <  1:2235066‑M2/41‑1 (MQ=255)

                                        gattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTaa                                                           <  1:2520391‑M2/42‑1 (MQ=255)

                                        gattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTaa                                                           >  2:287352‑M2/59‑100 (MQ=255)

                                           tttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTgttaagat                                                        >  2:2235076‑M2/56‑97 (MQ=255)

                                            ttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAg                                                       >  2:1623680‑M2/55‑100 (MQ=255)

                                            ttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAg                                                       <  2:3213893‑M2/46‑1 (MQ=255)

                                            ttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAg                                                       <  1:971771‑M2/46‑1 (MQ=255)

                                             tcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGt                                                      >  2:2491811‑M2/54‑100 (MQ=255)

                                              caataatgcgtgatgcagatcccacaaaacactcaattacttaacataaatgTATAAGTCATCATTTTGTTTTGGGTGTATTTTAATTTGTTAAAAAGt                                                      >  1:1017495‑M2/53‑99 (MQ=255)

                                               aataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGttt                                                    >  2:2667658‑M2/52‑100 (MQ=255)

                                                  aatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTGAATCTGTTAAAAAGTTTTTc                                                 <  2:3590544‑M2/52‑1 (MQ=255)

                                                  aatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTc                                                 <  2:971280‑M2/52‑1 (MQ=255)

                                                   atgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcg                                                <  2:695849‑M2/53‑1 (MQ=255)

                                                   atgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcg                                                >  2:2402057‑M2/48‑100 (MQ=255)

                                                   atgcgagatgcagatcacataaaacactcatttacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcg                                                <  1:1728598‑M2/53‑1 (MQ=255)

                                                    tgcgtgatgcatatcacacaaaacactcaattacttaacataatttTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgc                                               <  1:739159‑M2/54‑1 (MQ=255)

                                                    tgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgc                                               <  2:1401866‑M2/54‑1 (MQ=255)

                                                    tgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTGCAATCTGTTAAAAAGTTTTTcgc                                               <  1:2530274‑M2/54‑1 (MQ=255)

                                                      cgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgcta                                             <  1:391174‑M2/56‑1 (MQ=255)

                                                       gtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctac                                            >  1:1369710‑M2/44‑100 (MQ=255)

                                                         gatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacgc                                          >  2:2440770‑M2/42‑100 (MQ=255)

                                                         gatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacgc                                          <  1:1415915‑M2/59‑1 (MQ=255)

                                                           tgcatagcacacaaaacacttaattacttaacataaatgTATAAGTCATACTTTTGTTTTCGGTGTATTTCAATCTGTTAAAAAGTTTTTcgatacgc                                          <  1:3266983‑M2/59‑1 (MQ=255)

                                                           tgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacgcta                                        >  1:1171669‑M2/40‑100 (MQ=255)

                                                              agatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagca                                     <  2:117346‑M2/64‑1 (MQ=255)

                                                              agatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagca                                     >  1:2388966‑M2/37‑100 (MQ=255)

                                                                atcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCTCTACGCTagcaag                                   >  1:2822743‑M2/35‑100 (MQ=255)

                                                                atcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaag                                   <  1:2667645‑M2/66‑1 (MQ=255)

                                                                 tcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaagc                                  <  1:2005013‑M2/67‑1 (MQ=255)

                                                                  cacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaagca                                 <  1:2599414‑M2/68‑1 (MQ=255)

                                                                   ncacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAgcaagcaa                                >  1:2899789‑M2/32‑100 (MQ=255)

                                                                    cacaaaacactctattacttaacataaatgTATAAGTCATATTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaa                               <  1:361548‑M2/70‑1 (MQ=255)

                                                                      caaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaaa                             >  2:2476873‑M2/29‑100 (MQ=255)

                                                                      caaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaaa                             >  1:3426294‑M2/29‑100 (MQ=255)

                                                                       aaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGcaaaaan                            <  2:2899803‑M2/73‑2 (MQ=255)

                                                                        aaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATg                           <  2:2322370‑M2/74‑1 (MQ=255)

                                                                            actcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAAc                       <  2:3629972‑M2/78‑1 (MQ=255)

                                                                            actcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAAc                       <  1:997359‑M2/78‑1 (MQ=255)

                                                                             ctcaattacttaacataaatgTATAAGTCATACTTTTGTTTTTGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAAc                       <  2:2737446‑M2/78‑1 (MQ=255)

                                                                             ctcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACa                      >  2:3144971‑M2/22‑100 (MQ=255)

                                                                               caattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAgg                    <  2:2479649‑M2/81‑1 (MQ=255)

                                                                                   tacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaata                >  2:2872651‑M2/16‑100 (MQ=255)

                                                                                   tacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaata                <  2:423258‑M2/85‑1 (MQ=255)

                                                                                       taacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCg            >  2:1868699‑M2/12‑100 (MQ=255)

                                                                                       taaaaTAAAGGTATAAGACATACTTTTGTTTTGGGAATATTTCAATCTGTTAAAAAGTTTTTCGCTAAAAAAAAAAAAAAAAATGAAACAGGAATAATc             <  1:1631231/95‑1 (MQ=255)

                                                                                            taaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATg       >  2:1962375‑M2/7‑100 (MQ=255)

                                                                                            taaatgTATAAGTCATACTTTTGTTTTGGGGGTATTTCAATCTGGTAAAAAGTTGTTCGCTACGCTAGCAAGAAAAAATGAAACAGGAATAATCGAAAt        >  1:3750864‑M2/7‑99 (MQ=255)

                                                                                             aaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATgg      >  2:3079320‑M2/6‑100 (MQ=255)

                                                                                              aatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAACATGAAACAGGAATAATCGAAATggg     >  1:213295‑M2/5‑100 (MQ=255)

                                                                                              aatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATggg     <  1:1337500‑M2/96‑1 (MQ=255)

                                                                                              aatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATg       >  2:1490273‑M2/5‑98 (MQ=255)

                                                                                               atgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGa    <  2:2130029‑M2/97‑1 (MQ=255)

                                                                                                tgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCTCTATGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGAt   >  1:3607409‑M2/3‑100 (MQ=255)

                                                                                                tgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGAt   >  1:2455478‑M2/3‑100 (MQ=255)

                                                                                                 gTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATg  >  1:4346‑M2/2‑100 (MQ=255)

GAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATG  >  NC_000913/1979181‑1979377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`