Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,984 T→G 35.8% intergenic (‑194/+114) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9840TG35.8% 346.9 / 290.4 242intergenic (‑194/+114)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (78/77);  new base G (74/12);  total (153/89)
Fisher's exact test for biased strand distribution p-value = 1.57e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-02

Only 100 of 246 total aligned reads displayed.

TGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCT  >  NC_000913/3705886‑3706083
                                                                                                  |                                                                                                           
tgctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCtt                                                                                                            <  2:2874235/100‑1 (MQ=255)
 gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                                           >  1:721500/1‑100 (MQ=255)
 gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                                           >  1:2170085/1‑100 (MQ=255)
  ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                                          >  1:1994346/1‑100 (MQ=255)
  ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                                          >  1:1952097/1‑100 (MQ=255)
    gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                                        <  2:830180/100‑1 (MQ=255)
    gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                                        >  2:590897/1‑100 (MQ=255)
     ccGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                                       <  1:3371274/100‑1 (MQ=255)
      cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                                      <  2:2024779/100‑1 (MQ=255)
      cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCgg                                                                                                      <  2:2199478/100‑1 (MQ=37)
       ggATGGCGCTGCACGCAGAGGCACAGCGCTACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGc                                                                                                     <  1:367745/100‑1 (MQ=25)
       ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGc                                                                                                     <  2:654093/100‑1 (MQ=37)
        gATGGCGCGGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTGTGGTTATGCCGGATGCGGCGTTATCGCCTTATCCGGcc                                                                                                    <  1:1279210/100‑1 (MQ=25)
          tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATa                                                                                                  <  1:1804698/100‑1 (MQ=37)
          tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATa                                                                                                  <  2:3423502/100‑1 (MQ=37)
            gcgcTGCACGCAGAGGCACAGCGTCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                               <  1:511058/100‑1 (MQ=255)
            gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCAGCCTACa                                                                                               >  1:363104/1‑100 (MQ=255)
              gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCTGCGTTAACGCCTTATCCGGCCTACaaa                                                                                             >  1:935600/1‑100 (MQ=255)
              gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                                             >  2:3026780/1‑100 (MQ=255)
                 gCACGCAGAGGCACGGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                          >  1:2716316/1‑100 (MQ=255)
                  cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                                         <  2:2958412/100‑1 (MQ=255)
                  cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                                         <  2:573253/100‑1 (MQ=255)
                    cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                       <  2:2630196/100‑1 (MQ=255)
                    cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                       <  1:2450053/100‑1 (MQ=255)
                     gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                      <  2:1254493/100‑1 (MQ=255)
                        gagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCcaa                                                                                  >  1:798010/1‑100 (MQ=255)
                            cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                              >  1:2202194/1‑100 (MQ=255)
                              caGCGTCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                            <  2:1266067/100‑1 (MQ=255)
                               aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                                           <  2:1920368/100‑1 (MQ=255)
                                   ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                       <  2:2182920/100‑1 (MQ=255)
                                   ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGACGGATGCGGCGTTAACGCCTTATTCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                       >  1:1894181/1‑100 (MQ=255)
                                    cACCGGGTATACAGATTGTTGACAAAGTTCGTTTTGTTTATGCCGGATGCGGCGTTAACGCCTTACCCGGCCTACTAAATCGTGCAAATTCCATACAtgg                                                                      <  1:1386834/100‑3 (MQ=17)
                                     aCCGGGTATACACCTTGTTGACAAAGTCCGCTTTGTTTATCTCGGATGCGTCCTTAACGCCTTATCCGTCCTACAAAATCGTGCAAATTCATTACATTGc                                                                     <  1:313699/100‑1 (MQ=17)
                                             taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAAtttt                                                         >  1:2480225/1‑100 (MQ=255)
                                              aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTc                                                        <  1:3467121/100‑1 (MQ=255)
                                               cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑g                                                     <  2:1989021/100‑1 (MQ=255)
                                               cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑g                                                     <  2:2898213/100‑1 (MQ=255)
                                               cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑g                                                     <  1:2800359/100‑1 (MQ=255)
                                               cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑g                                                     <  2:492490/100‑1 (MQ=255)
                                                   ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAgg                                                 <  1:1917468/100‑1 (MQ=255)
                                                   ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAgg                                                 >  1:3510846/1‑100 (MQ=255)
                                                    tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGc                                                <  2:2242086/100‑1 (MQ=255)
                                                     gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGcc                                               >  2:161064/1‑100 (MQ=255)
                                                         aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGAt                                           <  2:1501042/100‑1 (MQ=255)
                                                          cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATa                                          >  1:3196531/1‑100 (MQ=255)
                                                          cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATAGTGCAAGTTCACTACATTGC‑‑AGACACTTC‑‑GTAGGCCTg                                             >  2:3182428/1‑97 (MQ=14)
                                                             aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGc                                       <  1:3751427/100‑1 (MQ=255)
                                                               tGCGCGTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCgg                                     <  1:1307701/100‑1 (MQ=255)
                                                                 cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAg                                   <  1:2709829/100‑1 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGTCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                                  >  1:2611423/1‑100 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGTTAAGCg                                      >  2:2423031/1‑97 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGa                                    <  1:2325865/98‑1 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                                  >  2:2063265/1‑100 (MQ=255)
                                                                     ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCa                               <  2:1240099/100‑1 (MQ=255)
                                                                     ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCa                               <  1:838261/100‑1 (MQ=255)
                                                                      tttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGC‑‑AGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCAt                              >  2:2698219/8‑100 (MQ=21)
                                                                       ttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑‑‑AATCTCCGTGTAGGCCTGATAAGCGTAGCGCATc                             >  2:1720692/7‑100 (MQ=18)
                                                                         ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg                           <  2:2716330/100‑1 (MQ=255)
                                                                           nggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACATACCGCGCAAATTCAATATATTGCGGAGAAAAT‑‑‑‑GTAGGCCTGATAAGCGTAGCGCGTCAGGct                        >  1:2043341/3‑99 (MQ=18)
                                                                           aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                          >  1:1429532/3‑99 (MQ=18)
                                                                           aTGCCGGATGCGGCGTTAACGCCGTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg                          <  1:1470/99‑1 (MQ=255)
                                                                            tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                        <  2:406035/100‑1 (MQ=255)
                                                                            tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                        >  1:1379054/1‑100 (MQ=255)
                                                                            tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                        >  2:1530808/1‑100 (MQ=255)
                                                                            ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGC‑‑AGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  1:386876/2‑100 (MQ=14)
                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGC‑‑AGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  2:1389603/2‑100 (MQ=18)
                                                                             gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                       >  2:1460892/1‑100 (MQ=255)
                                                                             gCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGC‑‑AGAAATCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                       >  2:2975010/1‑99 (MQ=18)
                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGC‑‑AAATCAACATGTAGGCCGGATAAGCGCAGCGCATCAGGCa                        >  1:2571710/1‑100 (MQ=18)
                                                                             gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGC‑‑AGGGACTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCag                       >  1:1217899/1‑99 (MQ=18)
                                                                              ccggataaggcgTTTACGCCGCATCCGGCCTACAAAACCGTGCTAATTCAATATATTGC‑‑AGAAACCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                      >  1:2031860/9‑98 (MQ=17)
                                                                              ccggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGC‑‑AGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                      >  2:3307202/9‑98 (MQ=18)
                                                                              ccggataaggcgTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGA‑‑TCGATCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                      >  2:105779/9‑98 (MQ=18)
                                                                              ccggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                      >  1:1152417/9‑98 (MQ=21)
                                                                              ccggataaggcgTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt                      >  2:1799584/9‑100 (MQ=18)
                                                                              ccGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGA‑‑TCGATCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                      >  1:3438258/1‑98 (MQ=18)
                                                                               cGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGATCGCATCAGGCAtt                      >  2:1094665/1‑99 (MQ=255)
                                                                                ggataaggcgTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGC‑‑GAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttgc                   >  1:1032296/7‑98 (MQ=18)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGC‑‑AGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  1:2235449/7‑100 (MQ=18)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGC‑‑AAGATTTTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCacgct                    >  2:732943/7‑96 (MQ=17)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGC‑‑AGGAGCTGA‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  1:868898/7‑100 (MQ=17)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGC‑‑AGAGATTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaatag                    >  1:2915465/7‑96 (MQ=17)
                                                                                 gataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGC‑‑AGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCCGGCAgttt                    >  1:1774301/6‑99 (MQ=17)
                                                                                  ataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGC‑‑ATGAGCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAGTtttg                  <  2:2145583/96‑1 (MQ=17)
                                                                                  aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                  <  1:2341624/100‑1 (MQ=255)
                                                                                  aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                  <  1:3025232/100‑1 (MQ=255)
                                                                                     cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct               <  1:3742334/100‑1 (MQ=255)
                                                                                      ggcgTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGC‑‑AGAGATCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAatttagcgtt              >  2:881092/1‑94 (MQ=17)
                                                                                      ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctt              >  2:3596102/1‑100 (MQ=255)
                                                                                         gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc           >  2:170255/1‑100 (MQ=255)
                                                                                          ttAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgcn          <  1:281638/100‑2 (MQ=255)
                                                                                            aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAt        >  1:1838723/1‑100 (MQ=255)
                                                                                             aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc       <  2:2451783/100‑1 (MQ=255)
                                                                                             aCGCCTTATCCGGACTACAAAATCGTGAAAATTAAATACATTGC‑‑AGCATTTTA‑‑GTAGGAGTGATAAGAGGAGCGCATCAGGCATTtttgcctttggtatc       >  1:2364465/1‑95 (MQ=18)
                                                                                              ccccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCCTCa      >  2:2576738/3‑100 (MQ=255)
                                                                                              cGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCa      >  1:2086127/1‑100 (MQ=255)
                                                                                              cGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGACTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCa      >  1:3549058/1‑100 (MQ=255)
                                                                                                ccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt    >  2:2224573/1‑100 (MQ=255)
                                                                                                 cTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTc   <  2:1773257/100‑1 (MQ=255)
                                                                                                  ttATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCt  <  2:1311195/100‑1 (MQ=255)
                                                                                                  |                                                                                                           
TGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCT  >  NC_000913/3705886‑3706083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: