Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,891,506 C→G 17.0% intergenic (+23/‑109) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,891,5060CG17.0% 447.9 / 80.4 171intergenic (+23/‑109)tnaB/mdtLtryptophan transporter of low affinity/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (78/64);  new base G (11/18);  total (89/82)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01

Only 100 of 176 total aligned reads displayed.

TTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGA  >  NC_000913/3891407‑3891603
                                                                                                   |                                                                                                 
ttGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTc                                                                                                   <  2:3078807/100‑1 (MQ=255)
ttGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTc                                                                                                   <  1:662779/100‑1 (MQ=255)
   tCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCt                                                                                                >  1:469496/1‑100 (MQ=255)
   tCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCt                                                                                                <  1:2535466/100‑1 (MQ=255)
    cATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTg                                                                                               >  1:1958700/1‑100 (MQ=255)
    cATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTg                                                                                               >  1:3278048/1‑100 (MQ=255)
     gatctcTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTgg                                                                                              <  1:723895/98‑1 (MQ=255)
     aTTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTgg                                                                                              <  1:212218/100‑1 (MQ=255)
     aTTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTgg                                                                                              <  2:140242/100‑1 (MQ=255)
      ttCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGc                                                                                             <  1:1277046/100‑1 (MQ=255)
       tctctcTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACTTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAAtggctggc                                                                                             <  1:3557342/99‑1 (MQ=255)
          ctTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGcttct                                                                                         <  1:1495028/100‑1 (MQ=255)
          ctTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGcttct                                                                                         <  1:666442/100‑1 (MQ=39)
          ctTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGcttct                                                                                         <  1:3006681/100‑1 (MQ=39)
          ctTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTggagatc                                                                                         >  2:723903/1‑95 (MQ=255)
          ctTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGcttct                                                                                         >  2:2158105/1‑100 (MQ=255)
           tttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGcttctt                                                                                        <  2:1445385/100‑1 (MQ=39)
           tttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGcttctt                                                                                        <  2:2760683/100‑1 (MQ=39)
           tttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGcttctt                                                                                        <  2:3015877/100‑1 (MQ=255)
            ttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGCTTCTTg                                                                                       <  2:620805/100‑1 (MQ=38)
            ttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGCTTCTTg                                                                                       <  2:392699/100‑1 (MQ=38)
            ttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTg                                                                                       <  2:3553469/100‑1 (MQ=255)
             tGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGCTTCTTga                                                                                      <  2:1791439/100‑2 (MQ=38)
             tGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGCTTCTTga                                                                                      <  1:3004894/100‑2 (MQ=38)
             tGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGCTTCTTg                                                                                       <  1:260391/99‑1 (MQ=255)
             tGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGc                                                                                      <  2:41201/100‑1 (MQ=255)
              ggTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGCTTCTTgaa                                                                                     <  1:1132331/100‑3 (MQ=38)
              ggTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGcc                                                                                     >  1:1407702/1‑100 (MQ=255)
               gTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGCTTCTTgaag                                                                                    <  2:3155120/100‑4 (MQ=38)
               gTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCct                                                                                    >  1:1636104/1‑100 (MQ=255)
                tataACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCctc                                                                                   >  2:3345320/1‑100 (MQ=255)
                    aCCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAg                                                                               <  2:1976907/100‑1 (MQ=255)
                     ccGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAgg                                                                              >  1:1714652/1‑100 (MQ=255)
                      cGTGATTTTGTGCTGTTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTGCATGAAGCCAGCGAATGGCTGGCTTCTTgaaggattt                                                                               <  1:3418567/98‑9 (MQ=25)
                      cGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTgggggcttcgg                                                                             >  2:1752323/1‑90 (MQ=255)
                      cGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAACCATTCGCTGGCTTCTTGCCTCTCAGGa                                                                             >  2:3477796/1‑100 (MQ=255)
                          aTTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATc                                                                         >  1:1945664/1‑100 (MQ=255)
                            tttGTGCTGGTTCGGCAACGTCTTTAACGTGTTTCCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCAc                                                                       <  1:1384969/100‑1 (MQ=255)
                            tttGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCAc                                                                       <  2:2333080/100‑1 (MQ=255)
                            tttGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCAc                                                                       <  1:2688984/100‑1 (MQ=255)
                              tgtgCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACtt                                                                     <  1:835141/100‑1 (MQ=255)
                                tgCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTAt                                                                   <  2:67943/100‑1 (MQ=255)
                                 gCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAAtagatcgga                                                                  >  1:3113602/1‑92 (MQ=255)
                                 gCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATg                                                                  >  1:2851477/1‑100 (MQ=255)
                                   tGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCGAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTc                                                                >  1:2444121/1‑100 (MQ=255)
                                    ggTTCGGCAACGTCTTTAACGTGTTACCTAACTTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTcc                                                               >  2:362422/1‑100 (MQ=255)
                                    ggTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGtag                                                               >  1:400111/1‑98 (MQ=255)
                                    ggTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTcc                                                               <  2:3173773/100‑1 (MQ=255)
                                     gTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCa                                                              <  2:3602798/100‑1 (MQ=255)
                                      ttCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCaa                                                             >  1:2253584/1‑100 (MQ=255)
                                        cGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAAt                                                           <  1:1848564/100‑1 (MQ=255)
                                           cAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGc                                                        <  2:687462/100‑1 (MQ=255)
                                           cAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGc                                                        <  1:939380/100‑1 (MQ=255)
                                            aaCGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCa                                                       <  2:1032917/100‑1 (MQ=255)
                                             aCGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCACGAAGCCAGCCATTCGCTCGCTTCTCGCCTCTCAGGAAATCACTTATGTCCAAATGGc                                                        <  1:3038098/98‑1 (MQ=255)
                                              cGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAAc                                                     >  1:2437992/1‑100 (MQ=255)
                                                 cTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGCCCAAATGGCAACTCg                                                  >  2:3754446/1‑100 (MQ=255)
                                                   ttAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGcc                                                <  2:1362851/100‑1 (MQ=255)
                                                       cGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAt                                            >  2:2975678/1‑100 (MQ=255)
                                                         tgtTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcc                                          <  1:1195073/100‑1 (MQ=255)
                                                         tgtTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcc                                          <  1:1012857/100‑1 (MQ=255)
                                                            tACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcctcc                                       >  2:3662953/1‑100 (MQ=255)
                                                            tACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcctcc                                       >  2:3114534/1‑100 (MQ=255)
                                                            tACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcctcc                                       >  2:3118131/1‑100 (MQ=255)
                                                            tACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcctcc                                       <  1:1072033/100‑1 (MQ=255)
                                                                tAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTca                                   >  2:452839/1‑100 (MQ=255)
                                                                    tttGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTcaccac                               >  1:536417/1‑100 (MQ=255)
                                                                       ggCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTa                            >  2:1225013/1‑100 (MQ=255)
                                                                        gCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGGCTCTCAGGCAAACACTTATGTCCACATGGCAACTTGCCTGATGCTCCTTaacccc                               >  1:3523099/1‑94 (MQ=255)
                                                                        gCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTAt                           >  2:2463209/1‑100 (MQ=255)
                                                                         cTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATg                          >  2:2472025/1‑100 (MQ=255)
                                                                          tAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATgn                         <  2:3358198/100‑2 (MQ=255)
                                                                          tAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATg                          <  1:1708511/99‑1 (MQ=255)
                                                                          tAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGc                         <  2:353915/100‑1 (MQ=255)
                                                                          tAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGc                         >  2:1072621/1‑100 (MQ=255)
                                                                           ctgagaggcAAGAGGCCAGCGAATGGCTCGCTTCTTGCCTCTCAAGAAATAACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCt                        >  1:1472649/9‑100 (MQ=255)
                                                                            aaTCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCtt                       >  2:827369/1‑100 (MQ=255)
                                                                             aTCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCttt                      >  1:628369/1‑100 (MQ=255)
                                                                               ccTTCAAGAAGCCAGCCATTCGCTGACTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGc                    >  2:2914022/1‑100 (MQ=255)
                                                                                cTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCCCGTATGCTTTgag                   <  2:2587872/100‑3 (MQ=255)
                                                                                cTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCg                   <  1:1568803/100‑1 (MQ=255)
                                                                                cTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCg                   >  1:462902/1‑100 (MQ=255)
                                                                                 ttCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGt                  <  2:3511656/100‑1 (MQ=255)
                                                                                 tccAAGAAGCGAGCCATTCGGTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCg                   <  1:3242791/97‑1 (MQ=255)
                                                                                  tCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTc                 <  2:2431344/100‑1 (MQ=255)
                                                                                  tCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTc                 <  1:1746315/100‑1 (MQ=255)
                                                                                  gcAAGAAGCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTc                 >  2:1245275/2‑100 (MQ=38)
                                                                                  gcAAGAAGCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATACTCCTTCACCACGTATGCTTTGCGTc                 >  2:2094678/2‑100 (MQ=38)
                                                                                   cAAGAAGCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACGACGTATGCTTTGCGTCa                >  1:1236102/1‑100 (MQ=38)
                                                                                   cAAGAAGCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCa                >  2:2174675/1‑100 (MQ=39)
                                                                                    aagaagCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCAc               >  2:59057/1‑100 (MQ=39)
                                                                                     agaagCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCAcc              >  2:1629936/1‑100 (MQ=39)
                                                                                     agaagCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCAcc              >  2:3310700/1‑100 (MQ=255)
                                                                                         gccagccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTAc          >  1:3227749/1‑100 (MQ=255)
                                                                                         gccagccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTAc          <  1:2724418/100‑1 (MQ=255)
                                                                                           cagccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTa        >  1:2942152/1‑100 (MQ=255)
                                                                                           cagccaTTCGCTGGCTTCTTGCCTCTAAGGAAATCACTTTTGTCCAAATGGCAACTCGCCTGATCCTCCCTCACCACGTATGCTTTGCGTCCACGTACTa        >  1:3719438/1‑100 (MQ=255)
                                                                                             gccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAAGTCGCCTGATCCTCCTGCACCACGTGTGCTTTGCGTCACCTTACTATc      >  1:2574821/1‑100 (MQ=255)
                                                                                             gccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATc      >  2:974129/1‑100 (MQ=255)
                                                                                                 ttCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGa  >  2:3436763/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
TTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGA  >  NC_000913/3891407‑3891603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: