BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,113,473	T→C	16.0%	L61L (TTG→CTG) ‡	uspD →	stress‑induced protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,113,473	0	T	C	16.0%	369.2 / 58.4	158	L61L (TTG→CTG)	uspD	stress‑induced protein
Reads supporting (aligned to +/- strand): ref base T (65/67); new base C (15/10); total (80/78)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 175 total aligned reads displayed.

GACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAA  >  NC_000913/4113375‑4113571

gACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAtt                                                                                                   >  1:3371128/1‑100 (MQ=255)

gACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAtt                                                                                                   >  2:2009049/1‑100 (MQ=255)

gACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAAAAGATATTCTTCAAtt                                                                                                   >  1:2229523/1‑100 (MQ=255)

  cATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAttgt                                                                                                 <  1:1339167/100‑1 (MQ=255)

   aTAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAttgtt                                                                                                <  1:3743944/100‑1 (MQ=255)

    tAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAttgttg                                                                                               >  2:1770768/1‑100 (MQ=255)

    tAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAttgttg                                                                                               <  1:3469240/100‑1 (MQ=255)

    tAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAttgttg                                                                                               >  1:1152445/1‑100 (MQ=255)

    tAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAttgttg                                                                                               >  2:83493/1‑100 (MQ=255)

    tAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAttgttg                                                                                               <  1:2925405/100‑1 (MQ=255)

    tAATGACGCTCACCTGACGTTAATGCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAttgttg                                                                                               <  2:1705162/100‑1 (MQ=255)

       tGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTgaag                                                                                            <  1:2561400/100‑1 (MQ=255)

       tGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATACTCTTCAATTGTTgaag                                                                                            >  1:2227819/1‑100 (MQ=255)

        gACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTgaaga                                                                                           <  2:2129603/100‑1 (MQ=255)

          cGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAACAATTGAAGAAt                                                                                         <  2:3440261/100‑1 (MQ=255)

          cGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGGTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAAt                                                                                         >  2:1657047/1‑100 (MQ=255)

           gCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATa                                                                                        <  1:25196/100‑1 (MQ=255)

           gCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATa                                                                                        <  2:2297651/100‑1 (MQ=255)

           gCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATa                                                                                        <  2:2749319/100‑1 (MQ=255)

              cACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGt                                                                                     >  1:3574723/1‑100 (MQ=255)

              cACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGt                                                                                     <  2:2110385/100‑1 (MQ=255)

              cACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAACAATTGAAGAATatct                                                                                     <  2:3389663/100‑4 (MQ=38)

              cACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAACAATTGAAGAATatct                                                                                     <  2:2227927/100‑4 (MQ=38)

                ccTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAACAATTGAAGAATatcttc                                                                                   <  2:486023/100‑6 (MQ=38)

                ccTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAACAATTGAAGAATatcttc                                                                                   <  1:1188918/100‑6 (MQ=38)

                 cTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCgg                                                                                  <  1:3685537/100‑1 (MQ=255)

                 cTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCgg                                                                                  <  2:2435547/100‑1 (MQ=255)

                 cTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCgg                                                                                  >  2:205100/1‑100 (MQ=255)

                 cTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAACAATTGAAGAATatcttct                                                                                  <  2:2529564/100‑7 (MQ=38)

                    aCGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATa                                                                               <  1:3062113/100‑1 (MQ=255)

                    aCGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTAAAGAATAAGTCGGATa                                                                               <  1:664784/100‑1 (MQ=255)

                     ngTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATaa                                                                              >  2:1196881/2‑100 (MQ=255)

                      gTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATaa                                                                              <  1:1904334/99‑1 (MQ=255)

                          aTTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGc                                                                         >  2:3775583/1‑100 (MQ=255)

                           ttCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCAGAtcggaagag                                                                        >  2:1250561/1‑92 (MQ=255)

                             cATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGt                                                                      >  1:1742736/1‑100 (MQ=255)

                              atatTTATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGAAAAAGAAGATATTCTTCATTTGTTGAAGAATAAGTAGAATAACAAACTGta                                                                     >  1:1235697/1‑100 (MQ=255)

                              atatTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGta                                                                     <  1:2700463/100‑1 (MQ=255)

                                atTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGtata                                                                   >  1:422084/1‑100 (MQ=255)

                                atTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGtata                                                                   <  2:2778049/100‑1 (MQ=255)

                                   gatgatGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAACAAGTCGGATAACCAGCTGTATAAAc                                                                <  2:2508452/100‑1 (MQ=255)

                                    atgatgGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACt                                                               <  2:3103449/100‑1 (MQ=255)

                                     tgatgGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTg                                                              <  1:2535933/100‑1 (MQ=255)

                                     tgatgGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTg                                                              >  1:1046904/1‑100 (MQ=255)

                                       atgGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGAc                                                            <  1:1082223/100‑1 (MQ=255)

                                       atgGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGAc                                                            <  2:279952/100‑1 (MQ=255)

                                       atgGCTTAAGCGAGTTGTATCCGGGTATCGACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGAc                                                            <  1:2656388/100‑1 (MQ=255)

                                        tgGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACg                                                           <  2:3660779/100‑1 (MQ=255)

                                            ttAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGaaaa                                                       <  2:2782090/100‑1 (MQ=255)

                                            ttAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGaaaa                                                       >  2:1889270/1‑100 (MQ=255)

                                               ctcGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAata                                                    <  2:1337958/98‑1 (MQ=255)

                                                 cGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAataag                                                  >  1:1337950/1‑98 (MQ=255)

                                                 cGAGTTGTATCCGGGTATCAACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAATAAGATAACAAGCTGTATAAACTGACGAAAAatat                                                   >  1:474789/1‑99 (MQ=255)

                                                    gTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCaa                                               >  1:1160562/1‑100 (MQ=255)

                                                      tGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAAGAAGCTGTATAAAATGACGAAAAATAGTAAATg                                             >  1:3349585/1‑100 (MQ=255)

                                                       gTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATgg                                            >  2:2597432/1‑100 (MQ=255)

                                                        tATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGc                                           >  2:2134131/1‑100 (MQ=255)

                                                        tATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGc                                           <  2:2149207/100‑1 (MQ=255)

                                                          tCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCg                                         <  2:3574744/100‑1 (MQ=255)

                                                          tCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCg                                         <  1:2795443/100‑1 (MQ=255)

                                                             gggTATCTACTTCCGTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAg                                      <  1:2597419/100‑1 (MQ=255)

                                                                tATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACa                                   >  2:3619410/1‑100 (MQ=255)

                                                                 aTCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACaa                                  <  2:2338563/100‑1 (MQ=255)

                                                                 aTCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAAAAAGTCAGATAACAACCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGAc                                    <  1:2354385/98‑1 (MQ=255)

                                                                 aTCTACTGCCCTGCAACAGAAGATATTCTTCAATTGTTAAAGAATAAGTCGGATAACAAGCTGTATAAAAAAAAGAAAAATATTCAATGGCCGAAGa                                     <  1:353291/97‑1 (MQ=255)

                                                                    tACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAAc                               >  2:3743228/1‑100 (MQ=255)

                                                                      cTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTg                             <  2:3132691/100‑1 (MQ=255)

                                                                       ttCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGc                            >  1:1739836/1‑100 (MQ=255)

                                                                        tCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCg                           <  2:3398705/100‑1 (MQ=255)

                                                                         tccTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGt                          <  1:3647762/99‑1 (MQ=255)

                                                                          ccTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTa                         >  2:187278/1‑100 (MQ=255)

                                                                           cTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTAt                        >  1:964320/1‑100 (MQ=255)

                                                                                aCAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAAc                   <  2:3554164/100‑1 (MQ=255)

                                                                                  agaagaTATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAAcgc                 <  1:863087/100‑1 (MQ=255)

                                                                                  agaagaTATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAAcgc                 >  2:121898/1‑100 (MQ=255)

                                                                                   gaagaTATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAAcgcg                >  1:926452/1‑100 (MQ=255)

                                                                                   gaagaTATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAAcgcg                >  2:2946284/1‑100 (MQ=255)

                                                                                   gaagaTATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAAcgcg                <  2:1142184/100‑1 (MQ=255)

                                                                                   cgacttatTCTTCAACAATTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAAcgcg                >  2:864815/6‑100 (MQ=38)

                                                                                   cgacttatTCTTCAACAATTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAAc                   >  2:1792739/6‑97 (MQ=38)

                                                                                    gacttatTCTTCAACAATTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATAGCCGAAGACAAAACTGCGTATTGAAcgcg                >  1:3395196/5‑99 (MQ=38)

                                                                                    aagaTATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCgg               <  2:1742745/100‑1 (MQ=255)

                                                                                     acttatTCTTCAACAATTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGga              >  2:759689/4‑100 (MQ=38)

                                                                                      cttatTCTTCAACAATTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGgag             >  1:1623408/3‑100 (MQ=255)

                                                                                      cttatTCTTCAACAATTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGgag             >  1:1962379/3‑100 (MQ=255)

                                                                                       ttatTCTTCAACAATTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGgaga            >  2:3004822/2‑100 (MQ=255)

                                                                                        tatTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGaa           >  2:406665/1‑100 (MQ=255)

                                                                                        tatTCTTCAACAATTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGaa           >  1:3265515/1‑100 (MQ=255)

                                                                                        tatTCTTCAACAATTGAAGAACAAGTCGGATAACAAGCCGTATAAACTGACGAAAAACATTCAATGGCCGAAGACAAAACTGCGTAATGAACGCGcaga            >  1:1155309/1‑99 (MQ=255)

                                                                                           tcttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATg        >  2:3416772/1‑100 (MQ=255)

                                                                                           tcttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATg        <  2:2286238/100‑1 (MQ=255)

                                                                                            cttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGc       >  1:1121212/1‑100 (MQ=255)

                                                                                            cttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGc       >  2:141841/1‑100 (MQ=255)

                                                                                             ttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGcc      >  1:3073768/1‑100 (MQ=255)

                                                                                             ttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGcc      >  2:3088062/1‑100 (MQ=255)

                                                                                               cAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCgg    >  1:3138331/1‑100 (MQ=255)

                                                                                               cAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCgg    <  2:1979008/100‑1 (MQ=255)

                                                                                               cAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCgg    <  2:436321/100‑1 (MQ=255)

                                                                                                aaTTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGa   <  1:1447161/100‑1 (MQ=255)

                                                                                                aaTTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAAATGACAAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGa   <  1:2470819/100‑1 (MQ=255)

GACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAA  >  NC_000913/4113375‑4113571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`