Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,376,291 T→C 15.3% intergenic (+26/‑26) efp → / → ecnA polyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,376,2910TC15.3% 437.6 / 64.6 183intergenic (+26/‑26)efp/ecnApolyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B
Reads supporting (aligned to +/- strand):  ref base T (85/70);  new base C (16/12);  total (101/82)
Fisher's exact test for biased strand distribution p-value = 8.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 186 total aligned reads displayed.

CGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTT  >  NC_000913/4376192‑4376390
                                                                                                   |                                                                                                   
  cTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGc                                                                                                   <  2:2561856/100‑1 (MQ=255)
   tGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCa                                                                                                  >  2:298850/1‑100 (MQ=255)
    gTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCAc                                                                                                 <  2:1186435/100‑1 (MQ=255)
    gTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCAc                                                                                                 >  1:459447/1‑100 (MQ=255)
    gTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAc                                                                                                 <  2:16072/100‑1 (MQ=255)
    gTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAACGCGGTTGTGGTGCAGCCTGCAGGCCGCAc                                                                                                 <  1:1858222/100‑1 (MQ=255)
     tttGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAcc                                                                                                <  1:3412577/100‑1 (MQ=255)
      ttGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCa                                                                                               >  2:268437/1‑100 (MQ=255)
      ttGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAcc                                                                                                <  1:2999128/99‑1 (MQ=255)
       tGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCAt                                                                                              <  1:1207047/100‑1 (MQ=255)
       tGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCAt                                                                                              >  2:2332505/1‑100 (MQ=255)
       tGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCACCac                                                                                              <  1:2918774/100‑2 (MQ=255)
        gTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATc                                                                                             <  1:1073693/100‑1 (MQ=255)
        gTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATc                                                                                             >  2:1940063/1‑100 (MQ=255)
        gTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCACCaca                                                                                             <  1:3154358/100‑3 (MQ=255)
        gTACAAAGCGGCGAAGTCATCAAAGTGGATACCCGCGCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATc                                                                                             <  1:1480608/100‑1 (MQ=255)
         tACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                            >  2:3347969/1‑100 (MQ=255)
         tACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                            >  2:664947/1‑100 (MQ=255)
         tACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCAGTTGTGGTGCGGCCTGCAGGCTGCACCATc                                                                                             >  1:2354377/1‑99 (MQ=255)
          aCAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCAc                                                                                           <  2:1738824/100‑1 (MQ=255)
            aaaTCGGCGAAGTCATCAAAGTGGATACCTGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCCCtt                                                                                         >  2:1691441/1‑100 (MQ=255)
                 ggCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCa                                                                                    <  2:3264994/100‑1 (MQ=255)
                 ggCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCa                                                                                    <  2:3437187/100‑1 (MQ=255)
                 ggCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCa                                                                                    >  1:3283660/1‑100 (MQ=255)
                     aaGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTc                                                                                <  1:1224083/100‑1 (MQ=255)
                       gTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAgg                                                                                  <  1:2263378/96‑1 (MQ=255)
                         catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCagag                                                                            >  1:217995/1‑100 (MQ=255)
                         catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCagag                                                                            >  1:1369873/1‑100 (MQ=255)
                         catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCagag                                                                            >  2:2145516/1‑100 (MQ=255)
                          atcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCagag                                                                            <  2:764361/99‑1 (MQ=255)
                           tcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgat                                                                          >  1:2618403/1‑100 (MQ=255)
                            caAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatg                                                                         <  1:2148157/100‑1 (MQ=255)
                             aaaGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatga                                                                        >  2:1247552/1‑100 (MQ=255)
                              aaGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatgat                                                                       <  2:1104922/100‑1 (MQ=255)
                                gTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGAtgatga                                                                     <  2:2033305/100‑1 (MQ=255)
                                  ngATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGaaa                                                                   >  2:813072/2‑100 (MQ=255)
                                     tACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGc                                                                <  2:3707918/100‑1 (MQ=255)
                                      aCCCGCTCTGGTGAATACGTCTCTCGTGTGATGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCGTCATCACTTAGTCAGGTCAGAGATGATGAAACGcc                                                               <  1:765488/100‑1 (MQ=255)
                                       cccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCt                                                              <  1:2391789/100‑1 (MQ=255)
                                          gCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTAt                                                           <  2:2807690/100‑1 (MQ=255)
                                             ctGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGt                                                        <  1:3768544/100‑1 (MQ=255)
                                               ggTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTGAGGTCAGAGATGATGAAACGCCTTATCGTTc                                                      >  1:2307164/1‑100 (MQ=255)
                                               ggTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGCAACGCCTTATCGTTc                                                      >  2:2448376/1‑100 (MQ=255)
                                               ggTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTc                                                      <  2:981214/100‑1 (MQ=255)
                                               ggTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTc                                                      >  1:3066009/1‑100 (MQ=255)
                                                gTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGt                                                        >  1:1252610/1‑97 (MQ=255)
                                                 tGAATATGTCTCTCGCTTGTATTCTTTCTGTTTTAGTTCGGTCTGTACGTTGCATCATCACTTATTCTAGTCAGAGATGATGAAACGCCTTAttattc                                                      <  1:3735630/98‑6 (MQ=255)
                                                  gAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTg                                                   <  1:3097291/100‑1 (MQ=255)
                                                  gAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTg                                                   >  1:3475222/1‑100 (MQ=255)
                                                   aaTACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGt                                                  <  2:66464/100‑1 (MQ=255)
                                                   aaTACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGCTCTTGt                                                  <  1:2767984/100‑1 (MQ=255)
                                                     tACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGttt                                                <  1:104795/100‑1 (MQ=255)
                                                      aCGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtttt                                               <  1:585483/100‑1 (MQ=255)
                                                         tctctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgct                                            >  2:497770/1‑100 (MQ=255)
                                                         tctctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgct                                            <  2:1894474/100‑1 (MQ=255)
                                                          ctctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgctt                                           <  2:1383961/100‑1 (MQ=255)
                                                           tctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttg                                          <  2:3475242/100‑1 (MQ=255)
                                                              cgcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCa                                       >  2:197571/1‑100 (MQ=255)
                                                               gcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAg                                      <  2:2418186/100‑1 (MQ=255)
                                                               gcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAg                                      >  2:68632/1‑100 (MQ=255)
                                                               gcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTACACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAg                                      >  1:2036833/1‑100 (MQ=255)
                                                                 gTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTCTTCAGGTCAGAGATGATGACACGCCTTATCGTTCTTGTTTTGCTTGCCAGCa                                    >  1:1873647/1‑100 (MQ=255)
                                                                 gTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCa                                    >  1:2709509/1‑100 (MQ=255)
                                                                   gAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCAc                                   >  2:2867008/1‑99 (MQ=255)
                                                                    aaGTAATGCGGTTGTGGTGCGGCCTGCAGGCTACACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgc                                 >  2:3618043/1‑100 (MQ=255)
                                                                     aGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgct                                >  2:3172017/1‑100 (MQ=255)
                                                                     aGTAATGCGGTTGTGGTGCCGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGCGATGATGAAACGCCTTATAGTTCTTGTTTTGCTTGCCCGCAc                                   >  1:3615476/1‑97 (MQ=255)
                                                                      gTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctg                               >  1:1103241/1‑100 (MQ=255)
                                                                       tAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctgc                              <  2:2595240/100‑1 (MQ=255)
                                                                        aaTGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctgct                             >  2:1863217/1‑100 (MQ=255)
                                                                        aaTGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctgct                             >  2:772958/1‑100 (MQ=255)
                                                                          tGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCa                           >  2:2048081/1‑100 (MQ=255)
                                                                          tGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCa                           >  2:1664789/1‑100 (MQ=255)
                                                                          tGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCa                           >  2:470813/1‑100 (MQ=255)
                                                                          ataagtgatggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGCTCTTGTTTTGCTTGCCAGCACGCTGCTCa                           >  2:2946672/9‑100 (MQ=37)
                                                                            cagTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACg                         >  2:2579664/3‑100 (MQ=255)
                                                                            cGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACg                         >  2:3014793/1‑100 (MQ=255)
                                                                             agtgatggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACgg                        >  2:1825513/6‑100 (MQ=38)
                                                                              gTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACggg                       >  1:3565054/1‑100 (MQ=255)
                                                                               tgatggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGc                      >  2:902125/4‑100 (MQ=255)
                                                                                natggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTCTCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCt                     >  1:71600/3‑100 (MQ=255)
                                                                                gatggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCt                     >  2:3157779/3‑100 (MQ=255)
                                                                                 gtggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTg                    <  2:740855/100‑1 (MQ=255)
                                                                                 gtggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTg                    <  2:1923971/100‑1 (MQ=255)
                                                                                 gtggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTg                    <  2:1929709/100‑1 (MQ=255)
                                                                                 atggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTg                    >  1:1708612/2‑100 (MQ=255)
                                                                                   ggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTa                  >  2:3074364/1‑100 (MQ=255)
                                                                                   ggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTa                  >  2:293831/1‑100 (MQ=255)
                                                                                   ggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTa                  <  1:3302181/100‑1 (MQ=255)
                                                                                   ggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTa                  >  2:1416438/1‑100 (MQ=255)
                                                                                    gtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTaa                 <  1:2444614/100‑1 (MQ=255)
                                                                                    gtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTaa                 >  1:391/1‑100 (MQ=255)
                                                                                    gtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTaa                 >  2:1306833/1‑100 (MQ=255)
                                                                                        ggCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACAcc             <  1:623797/100‑1 (MQ=255)
                                                                                        ggCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACAcc             <  1:197567/100‑1 (MQ=255)
                                                                                        ggCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACAcc             <  2:2208480/100‑1 (MQ=255)
                                                                                        agcctgcagcctgcaCCATCACTCATTCAGGTCAGAGATGATGAAACGCCTTATCATTCTTGTTTTGCTTGCCAGCCCGCTGCCCACCTGCTGTCACAcc             >  1:1943368/2‑100 (MQ=255)
                                                                                         gCCTGCAGGCTGCACCATCACTTATTTAGGTCAGAGATGATGAAACGCCTTATCGTTCTTTTTTTGCTTGCCAGCACGCTGCCCACGGGCGGTAACACCg            >  1:645702/1‑100 (MQ=255)
                                                                                         gCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTATTGCTGGCCAGCACACTGCTCACGGGCTGTAACACCg            >  1:2572251/1‑100 (MQ=255)
                                                                                             gCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTc         >  1:2659112/1‑99 (MQ=255)
                                                                                                   |                                                                                                   
CGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTT  >  NC_000913/4376192‑4376390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: