Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,298,278 T→A 15.5% intergenic (‑157/‑320) adhE ← / → ychE fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase/UPF0056 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,298,2780TA15.5% 319.2 / 50.5 129intergenic (‑157/‑320)adhE/ychEfused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase/UPF0056 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (55/54);  new base A (10/10);  total (65/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.24e-01

Only 100 of 138 total aligned reads displayed.

TATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGACA  >  NC_000913/1298179‑1298376
                                                                                                   |                                                                                                  
tataGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGt                                                                                                    >  2:2042249/1‑100 (MQ=255)
  taGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGTTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGtta                                                                                                  <  1:1304695/100‑1 (MQ=255)
  taGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGtta                                                                                                  <  1:1307379/100‑1 (MQ=255)
  taGTAAGTGACGTGATCATTTGCTTAACCTATGAAATTCTAAAGCGTACTCGCGCTCACACTCACTGTGATGGATTAAAATAGCTTAAACAGGAGAGtta                                                                                                  >  1:2610874/1‑100 (MQ=255)
     tCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGttatta                                                                                               <  1:2932018/100‑1 (MQ=255)
     tCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGttatta                                                                                               <  2:697019/100‑1 (MQ=255)
       aGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTAtc                                                                                             >  2:64449/1‑100 (MQ=255)
         tGCAGTGATGATTTGCTTAACCTATGAAAAACAAAAACTTACTCGGACTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTAtctc                                                                                           <  1:368172/100‑1 (MQ=255)
          gctgTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATctct                                                                                          <  1:3422751/97‑1 (MQ=255)
          gCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATctct                                                                                          <  2:2778616/100‑1 (MQ=255)
          gCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATctct                                                                                          <  2:1711843/100‑1 (MQ=255)
          gCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATctct                                                                                          <  2:1149965/100‑1 (MQ=255)
            ggTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTaa                                                                                        >  2:2757071/1‑100 (MQ=255)
             gTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAAt                                                                                       >  1:1327354/1‑100 (MQ=255)
             gTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAAt                                                                                       <  1:1441277/100‑1 (MQ=255)
              tgatgaTTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATg                                                                                      >  2:3193268/1‑100 (MQ=255)
               gatgatTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGc                                                                                     >  1:3056424/1‑100 (MQ=255)
                atgatTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGATAATAACTCTAATgtt                                                                                    <  2:3563802/100‑3 (MQ=255)
                  gatTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGt                                                                                   >  1:2252526/1‑99 (MQ=255)
                  gatTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTc                                                                                  >  1:422745/1‑100 (MQ=255)
                   atTTGCTTACCCTATGAAGTTCAAATGCTTACTCGCGCTCACACTCACTGTGATTTACTAAGAGAGTTTAAACATTATAGATAGGAACTCTAATgtttaa                                                                                 <  1:357233/100‑6 (MQ=255)
                    tttGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCAc                                                                                <  2:300696/100‑1 (MQ=255)
                     ttGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGATAATAACTCTAATgtttaaac                                                                               <  2:1836673/100‑8 (MQ=37)
                     ttGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGATAATAACTCTAATgtttaaac                                                                               <  1:1676709/100‑8 (MQ=38)
                     ttGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGATAATAACTCTAATgtttaaac                                                                               <  2:578800/100‑8 (MQ=37)
                     ttGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGATAATAACTCTAATgtttaaac                                                                               <  2:1359079/100‑8 (MQ=38)
                      tGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGATAATAACTCTAATgtttaaact                                                                              <  2:2158454/100‑9 (MQ=37)
                       gCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTc                                                                             >  1:1548292/1‑100 (MQ=255)
                         ttAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCa                                                                           <  1:1187194/100‑1 (MQ=255)
                          tAACCTATGAAAATCAAAAGCTTACTCGCGCTAACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAg                                                                          >  2:3623163/1‑100 (MQ=255)
                           aaCCTACGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAgg                                                                         <  2:173613/100‑1 (MQ=255)
                             ccTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTg                                                                       >  2:1092095/1‑100 (MQ=255)
                              cTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTgg                                                                      <  1:2729050/100‑1 (MQ=255)
                                aTGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCg                                                                    <  2:595584/100‑1 (MQ=255)
                                aTGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCg                                                                    >  2:1804300/1‑100 (MQ=255)
                                 tGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGt                                                                   >  1:1611661/1‑100 (MQ=255)
                                  gAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTa                                                                  <  2:3454085/100‑1 (MQ=255)
                                  gAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTa                                                                  <  2:2448915/100‑1 (MQ=255)
                                   aaaaTCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTaa                                                                 >  1:3402751/1‑100 (MQ=255)
                                   aaaaTCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTaa                                                                 <  1:2082783/100‑1 (MQ=255)
                                    aaaTCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTCAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGACGTacg                                                                >  1:1778545/1‑98 (MQ=255)
                                    aaaTCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAg                                                                <  2:2429695/100‑1 (MQ=255)
                                    aaaTCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAg                                                                <  1:3717370/100‑1 (MQ=255)
                                     aaTCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGc                                                               >  2:2216258/1‑100 (MQ=255)
                                       tCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCaa                                                             >  2:2663320/1‑100 (MQ=255)
                                              cTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACt                                                      <  2:3436425/100‑1 (MQ=255)
                                               ttACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTc                                                     <  1:2249178/100‑1 (MQ=255)
                                               ttACTCGCGCTCAAACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTc                                                     <  1:2461902/100‑1 (MQ=255)
                                                tACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACGTTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCa                                                    <  1:437288/100‑1 (MQ=255)
                                                 aCTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCAc                                                   <  2:276702/100‑1 (MQ=255)
                                                  cTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACt                                                  <  1:2142506/100‑1 (MQ=255)
                                                    cgcgCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTc                                                >  2:604340/1‑100 (MQ=255)
                                                    cgcgCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTc                                                >  1:3132741/1‑100 (MQ=255)
                                                    cgcgCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTc                                                >  1:2901883/1‑100 (MQ=255)
                                                    cgcgCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTACAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTc                                                >  1:212417/1‑100 (MQ=255)
                                                      cgcTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTg                                              >  1:344725/1‑100 (MQ=255)
                                                        cTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTggg                                            <  2:741804/100‑1 (MQ=255)
                                                           acacTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTAc                                         <  2:3699391/100‑1 (MQ=255)
                                                            cactcactGTGATTTACTAACAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACt                                        >  1:2322131/1‑100 (MQ=255)
                                                            cactcactGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACt                                        >  2:1991081/1‑100 (MQ=255)
                                                              ctcactGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGa                                      >  1:18640/1‑100 (MQ=255)
                                                              ctcactGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGa                                      >  1:1194332/1‑100 (MQ=255)
                                                                  ctGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTAc                                  <  2:3666011/100‑1 (MQ=255)
                                                                    gtgATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATACGTCACTTCCAGGTGGCGTAAGAAAGATTACTCACTTCTGGGTACTGATTACg                                 >  1:2456055/1‑99 (MQ=255)
                                                                       aTTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGAt                             >  2:2329675/1‑100 (MQ=255)
                                                                         ttACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATcc                           <  1:256127/100‑1 (MQ=255)
                                                                          tACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCca                          <  2:3559544/100‑1 (MQ=255)
                                                                           aCTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaa                         <  1:1067987/100‑1 (MQ=255)
                                                                            cTAAAAGAGTTTAAACATTAGACTTATCATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGAGTAAGTGATCcaaa                        >  1:2555200/1‑100 (MQ=255)
                                                                             tAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaaat                       <  2:3341351/100‑1 (MQ=255)
                                                                              aaaaGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaaatc                      <  2:1448400/100‑1 (MQ=255)
                                                                              aaaaGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTAAATTCTGGATACTGATTACGTGATAcaaa                        >  1:3132047/1‑98 (MQ=255)
                                                                              aaaaGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGAGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaaatc                      >  2:1154359/1‑100 (MQ=255)
                                                                                 ggaagtgacgcATTAGAGATAATAACTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaaatcaaa                   >  2:600875/11‑100 (MQ=37)
                                                                                    gTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAAttt                >  1:2836417/1‑100 (MQ=255)
                                                                                    gTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAAttt                >  1:1035626/1‑100 (MQ=255)
                                                                                     tttAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAATATTACTCACTTCTGGGTACTGATTACGTGATCCAAATTAAAttt                >  1:941151/1‑99 (MQ=255)
                                                                                      ttAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAAttttt              <  2:1194339/100‑1 (MQ=255)
                                                                                       tAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTCCTGATTACGTGATCCAAATCAAAttttt              >  2:1005056/1‑99 (MQ=255)
                                                                                       tAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAAttttt              <  2:3599512/99‑1 (MQ=255)
                                                                                       tAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTg             <  2:795981/100‑1 (MQ=255)
                                                                                       tAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTg             <  1:805582/100‑1 (MQ=255)
                                                                                       tAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTg             <  2:3690951/100‑1 (MQ=255)
                                                                                       gacgcATTAGAGATAATAACTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTg             >  2:3113661/5‑100 (MQ=38)
                                                                                       gacgcATTAGAGATAATAACTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTg             >  1:1395974/5‑100 (MQ=38)
                                                                                       gacgcATTAGAGATAATAACTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTg             >  1:687604/5‑100 (MQ=38)
                                                                                         cgcATTAGAGATAATAACTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCa           >  2:71588/3‑100 (MQ=255)
                                                                                         cgcATTAGAGATAATAACTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCa           >  1:3560937/3‑100 (MQ=255)
                                                                                         aaCATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCa           <  1:1991071/100‑1 (MQ=255)
                                                                                         aaCATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCa           <  1:686077/100‑1 (MQ=255)
                                                                                         aaCATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACATCTGGGCACTGATTACGTGATCCAAATCAAATTTTTGCa           >  1:2676872/1‑100 (MQ=255)
                                                                                           cATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCaaa         >  2:90017/1‑100 (MQ=255)
                                                                                           cATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCaaa         <  2:1742459/100‑1 (MQ=255)
                                                                                             ttAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGc       <  1:1006383/100‑1 (MQ=255)
                                                                                             ttAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGc       <  2:1790922/100‑1 (MQ=255)
                                                                                               agagTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTg     >  2:2442274/1‑100 (MQ=255)
                                                                                                gagTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGa    >  2:2553152/1‑100 (MQ=255)
                                                                                                 agTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGac   >  1:3637437/1‑100 (MQ=255)
                                                                                                  gTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGaca  >  1:682439/1‑100 (MQ=255)
                                                                                                  gTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGaca  >  2:1035839/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
TATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGACA  >  NC_000913/1298179‑1298376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: