Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,999,231 T→A 15.6% T84S (ACT→TCT)  dcyD ← D‑cysteine desulfhydrase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,999,2310TA15.6% 373.6 / 53.7 147T84S (ACT→TCT) dcyDD‑cysteine desulfhydrase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (64/60);  new base A (11/12);  total (75/72)
Fisher's exact test for biased strand distribution p-value = 8.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 151 total aligned reads displayed.

AGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATT  >  NC_000913/1999132‑1999329
                                                                                                   |                                                                                                  
aGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGCTTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGt                                                                                                    >  1:1226982/1‑100 (MQ=255)
aGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGt                                                                                                    >  2:139511/1‑100 (MQ=255)
    aTTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGc                                                                                                <  2:2807203/100‑1 (MQ=255)
     ttGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGACAGCGACTGCGGCAGTCTGcc                                                                                               <  2:399279/100‑1 (MQ=255)
       gCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGc                                                                                             >  2:156834/1‑100 (MQ=255)
        ccGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGca                                                                                            >  2:905015/1‑100 (MQ=255)
          gTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGcaca                                                                                          <  1:1392556/100‑1 (MQ=255)
          gTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGACTGCCGcagt                                                                                          <  1:3599854/100‑3 (MQ=255)
           ttGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACAt                                                                                         <  1:3074374/100‑1 (MQ=255)
           ttGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACAt                                                                                         <  1:203887/100‑1 (MQ=255)
           ttGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGACTGCCGcagtc                                                                                         <  1:2326330/100‑4 (MQ=255)
            tGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGACTGCCGcagtcg                                                                                        <  2:1273190/100‑5 (MQ=255)
            tGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGACTGCCGcagtcg                                                                                        <  2:3434678/100‑5 (MQ=255)
            tGGTTAAATAGTTTTCTGCGGTTGTACCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATg                                                                                        >  2:699927/1‑100 (MQ=255)
             ggTTAAATAGTTTTCTGCGGTTGTGCCAATCGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATgg                                                                                       <  2:2370142/100‑1 (MQ=255)
             ggTTAAATAGTTTTCTGCGGTTGTGCCAATATGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGACTGCCGcagtcgc                                                                                       <  1:3043229/100‑6 (MQ=255)
             ggTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATgg                                                                                       >  1:2951268/1‑100 (MQ=255)
             ggTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATgg                                                                                       <  2:1593539/100‑1 (MQ=255)
              gTTAAATGGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGt                                                                                      >  2:2168633/1‑100 (MQ=255)
              gTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGt                                                                                      >  2:1094980/1‑100 (MQ=255)
              gTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGACTGCCGcagtcgct                                                                                      <  2:64547/100‑7 (MQ=255)
               ttAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGtt                                                                                     <  1:1040052/100‑1 (MQ=255)
               ttAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGtt                                                                                     <  2:1247248/100‑1 (MQ=255)
                 aaaTAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAg                                                                                   >  2:1966992/1‑100 (MQ=255)
                 aaaTAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAg                                                                                   <  1:474956/100‑1 (MQ=255)
                  aaTAGTGTTCTGCGATCATGCCAATAGGATTTTCCAGCAGCGATACGCAGTGCAGACAGAGGGTGGCAGCGACTGCGGGAGTCTGCCGCACATGGTGAg                                                                                   >  1:3131018/1‑99 (MQ=255)
                   aTAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGAc                                                                                 <  2:3812343/100‑1 (MQ=255)
                    tAGTTTTCTGCGGTTGTGCCAATAGGATTTTCGAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACt                                                                                >  1:349711/1‑100 (MQ=255)
                        tttCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGAt                                                                            <  2:2185304/100‑1 (MQ=255)
                          tCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCg                                                                          <  2:2152862/100‑1 (MQ=255)
                          tCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCg                                                                          <  2:1078573/100‑1 (MQ=255)
                           cTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGc                                                                         >  2:1455555/1‑100 (MQ=255)
                           cTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGc                                                                         >  1:928614/1‑100 (MQ=255)
                            tGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGcc                                                                        >  2:2144471/1‑100 (MQ=255)
                             gCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGccc                                                                       <  1:650785/100‑1 (MQ=255)
                              cGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGcccc                                                                      <  2:671428/100‑1 (MQ=255)
                               ggTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCg                                                                     <  2:3508882/100‑1 (MQ=255)
                               ggTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCg                                                                     >  2:357453/1‑100 (MQ=255)
                                 ttGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGc                                                                   <  1:1517942/100‑1 (MQ=255)
                                 ttGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGc                                                                   <  1:778466/100‑1 (MQ=255)
                                  tgtgCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCa                                                                  >  1:2830623/1‑100 (MQ=255)
                                   gtgCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAg                                                                 >  1:639738/1‑100 (MQ=255)
                                    tgCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCATTTTTCCGCTTTTTTTTTTTCTGGATCGCCCCGGCAGt                                                                >  1:1171719/1‑100 (MQ=255)
                                    tgCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGt                                                                <  1:1542541/100‑1 (MQ=255)
                                    tgCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAACGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGt                                                                >  1:1370724/1‑100 (MQ=255)
                                      ccAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTaa                                                              >  1:2264758/1‑100 (MQ=255)
                                      ccAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTaa                                                              <  2:2608317/100‑1 (MQ=255)
                                         aTAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCa                                                           >  1:1720201/1‑100 (MQ=255)
                                          tAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAg                                                          <  2:618939/100‑1 (MQ=255)
                                            ggATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGGGTGCGGCAGTCTGCCGCACAAGGTTAGACTGGAGCGCCCCGGCAGTAGTCGGCg                                                        >  1:450760/1‑100 (MQ=255)
                                            ggATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCg                                                        <  2:862221/100‑1 (MQ=255)
                                            ggATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCCGCCGCACATGGTTAGACTGGATCGCCCCAGCAGTAATCAgtg                                                        <  2:2576184/100‑3 (MQ=255)
                                                 ttCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCg                                                   >  2:1627777/1‑100 (MQ=255)
                                                 ttCCAGCAGCGCCACGCAGTGACGACCGAGTTTGGCAGCGCCTGCGGCAGTCTGCCGAACATGGTTAGACTGGATCGCCCCGGCAGGAATCa                                                           <  1:1605465/92‑1 (MQ=255)
                                                  tCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCgg                                                  >  2:2405753/1‑100 (MQ=255)
                                                    cagcagCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCa                                                <  2:778427/100‑1 (MQ=255)
                                                       cagcGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCt                                             <  2:3235412/100‑1 (MQ=255)
                                                           gcCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCAc                                         >  2:1521754/1‑100 (MQ=255)
                                                           gcCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCAc                                         <  2:271188/100‑1 (MQ=255)
                                                           gcCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCAc                                         >  1:752564/1‑100 (MQ=255)
                                                            ccACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATTGTTAGACTCGATAGCCCCGGCAGTGATCAACGTATCGGAACCtt                                            >  1:3622660/1‑96 (MQ=255)
                                                            ccACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACg                                        <  1:3301158/100‑1 (MQ=255)
                                                            ccACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACg                                        >  2:281250/1‑100 (MQ=255)
                                                            ccACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACg                                        >  1:409109/1‑100 (MQ=255)
                                                             cACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGc                                       >  1:1201892/1‑100 (MQ=255)
                                                              aCGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCa                                      >  1:192953/1‑100 (MQ=255)
                                                                gCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCaga                                    <  1:2082838/100‑1 (MQ=255)
                                                                 cAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCagag                                   <  1:2547214/100‑1 (MQ=255)
                                                                 cAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCagag                                   <  1:1937380/100‑1 (MQ=255)
                                                                 cAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCagag                                   <  2:3026662/100‑1 (MQ=255)
                                                                   gTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCa                                 >  1:2720139/1‑100 (MQ=255)
                                                                   gTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCa                                 >  1:2161190/1‑100 (MQ=255)
                                                                    tGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCAt                                >  2:889323/1‑100 (MQ=255)
                                                                     gCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATc                               >  1:1560868/1‑100 (MQ=255)
                                                                        gACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGc                            >  2:176226/1‑100 (MQ=255)
                                                                         aCCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGcc                           >  2:1600545/1‑100 (MQ=255)
                                                                           cGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCcgc                         >  2:3030443/1‑100 (MQ=255)
                                                                               tttGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCgaga                     <  2:1292575/100‑1 (MQ=255)
                                                                                 tGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGaaa                   >  1:2427481/1‑100 (MQ=255)
                                                                                   taaccatgtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAAtt                 >  1:370248/9‑100 (MQ=255)
                                                                                   gCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGaaact                 >  2:1664780/1‑98 (MQ=255)
                                                                                    cAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCATCGTATCGGCCCCTTCACGCAGAGCATCTGCCGCGAGAAATTc                >  2:1758792/1‑100 (MQ=255)
                                                                                    cAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGCAAtt                 >  1:3516466/1‑99 (MQ=255)
                                                                                    aaccatgtGCGGCAGACCGACGCACATGGTTAGACTCGATCGCCCAGGCAGTCCTCAGCGGATCGGCACCTTCACGCAGAGCATCAGCCCCga                       >  1:762966/8‑93 (MQ=255)
                                                                                     aGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTcc               <  1:799080/100‑1 (MQ=255)
                                                                                      ccatgtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCa              >  2:3228770/6‑100 (MQ=255)
                                                                                      ccatgtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCa              >  2:1048387/6‑100 (MQ=255)
                                                                                       catgtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAg             >  2:535468/5‑100 (MQ=255)
                                                                                       catgtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAg             >  2:627262/5‑100 (MQ=255)
                                                                                       cGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAg             <  2:2279460/100‑1 (MQ=255)
                                                                                       cGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAg             <  2:110603/100‑1 (MQ=255)
                                                                                        gACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGc            <  2:2911646/100‑1 (MQ=255)
                                                                                         tgtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCt           >  1:1646883/3‑100 (MQ=255)
                                                                                         aCTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCt           >  2:1904325/1‑100 (MQ=255)
                                                                                          ctcttccgatCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCtt          <  1:2737273/91‑1 (MQ=255)
                                                                                          cTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCtt          >  2:2907036/1‑100 (MQ=255)
                                                                                           tGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTa         <  2:1011496/100‑1 (MQ=255)
                                                                                            gCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTAc        >  2:928394/1‑100 (MQ=255)
                                                                                              ggCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGt      >  2:3720250/1‑100 (MQ=255)
                                                                                               gCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTa     >  1:2128614/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
AGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATT  >  NC_000913/1999132‑1999329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: