Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,391,414 C→G 16.1% R31P (CGA→CCA)  nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,391,4140CG16.1% 326.3 / 51.6 124R31P (CGA→CCA) nuoNNADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base C (51/53);  new base G (7/13);  total (58/66)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

Only 100 of 129 total aligned reads displayed.

GTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGA  >  NC_000913/2391315‑2391510
                                                                                                   |                                                                                                
gTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTc                                                                                                  <  1:999906/100‑1 (MQ=255)
gTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTc                                                                                                  <  1:3109015/100‑1 (MQ=255)
gTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTc                                                                                                  <  2:2795653/100‑1 (MQ=255)
gTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTc                                                                                                  >  2:2599999/1‑100 (MQ=255)
gTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTc                                                                                                  <  2:2595228/100‑1 (MQ=255)
 tAACGTCCATAGCGCCCGCCTGGTCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCg                                                                                                 <  1:543191/100‑1 (MQ=255)
 tAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCg                                                                                                 >  1:2715510/1‑100 (MQ=255)
 tAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCg                                                                                                 >  2:3113926/1‑100 (MQ=255)
   aCGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGcc                                                                                               >  2:3125383/1‑100 (MQ=255)
     gTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCAc                                                                                             >  1:2485802/1‑100 (MQ=255)
     gTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCAc                                                                                             >  1:34305/1‑100 (MQ=255)
        cacaGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGGGGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGc                                                                                           <  1:544966/96‑1 (MQ=255)
        cATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCa                                                                                          >  2:1167607/1‑100 (MQ=255)
        cATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCa                                                                                          <  1:1741580/100‑1 (MQ=255)
        cATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCa                                                                                          >  2:1681827/1‑100 (MQ=255)
        cATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCa                                                                                          >  2:727874/1‑100 (MQ=255)
         aTAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCaa                                                                                         >  2:2620181/1‑100 (MQ=255)
           aGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATg                                                                                       <  1:727866/100‑1 (MQ=255)
            gcgcCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtca                                                                                      <  1:2964893/100‑3 (MQ=255)
            gcgcCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATgg                                                                                      >  1:2195091/1‑100 (MQ=255)
             cgcccgccTGGCCAACAAACCAGGGCGAAACCAGCGCCGCGTTAAGCCCCATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtca                                                                                      <  1:454148/99‑3 (MQ=255)
             cgcccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcat                                                                                     <  2:892595/100‑4 (MQ=255)
             cgcccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcat                                                                                     <  2:1114740/100‑4 (MQ=255)
             cgcccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGga                                                                                     >  2:3005588/1‑100 (MQ=255)
              gcccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatt                                                                                    <  1:3666221/100‑5 (MQ=255)
              gcccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGgag                                                                                    <  1:995643/100‑1 (MQ=255)
               cccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcattt                                                                                   <  1:3133165/100‑6 (MQ=255)
                ccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGagag                                                                                  <  2:2149427/100‑1 (MQ=255)
                 cgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttcc                                                                                 <  1:2150407/100‑8 (MQ=255)
                 cgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGc                                                                                 >  2:2023823/1‑100 (MQ=255)
                  gccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttcct                                                                                <  1:2160650/100‑9 (MQ=38)
                  gccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttcct                                                                                <  1:1912757/100‑9 (MQ=38)
                  gccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCa                                                                                <  1:530299/100‑1 (MQ=255)
                   ccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttcctc                                                                               <  1:2113695/100‑10 (MQ=38)
                    cTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATc                                                                              <  1:3316920/100‑1 (MQ=255)
                         caacaaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTCGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACaa                                                                          >  1:2487952/1‑99 (MQ=255)
                          aacaaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAacc                                                                        <  2:2282854/100‑1 (MQ=255)
                           acaaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAacca                                                                       <  1:2894021/100‑1 (MQ=255)
                              aaCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGGTGAGGAAATGATTGCGTCGCCACGCAACGGAGAGCATCACAaccacca                                                                    >  1:2476421/1‑100 (MQ=255)
                               aCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGGAATGAGTGCGTCGCCCCGCAAAGGAGAGCATCACAaccaccac                                                                   >  1:223174/1‑100 (MQ=255)
                               aCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAaccaccac                                                                   >  2:536670/1‑100 (MQ=255)
                                ccAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAaccaccacc                                                                  >  2:1120619/1‑100 (MQ=255)
                                 cAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCg                                                                 <  2:2934306/100‑1 (MQ=255)
                                 cAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCg                                                                 <  2:117813/100‑1 (MQ=255)
                                   gagCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTc                                                               <  2:1525536/100‑1 (MQ=255)
                                     gCGAAACCAGCGCAGCGTTAAGCCCAATAACCGAGAGCTTAGCGTAGCGGAAACGATCGCGGCCGCACGCAATGGAGAGCCTCACAACCGCCACCGGCaa                                                             >  1:2354808/1‑100 (MQ=255)
                                      cGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGTGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAg                                                            <  1:2586279/100‑1 (MQ=255)
                                       gAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGc                                                           <  1:2548834/100‑1 (MQ=255)
                                       gAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGc                                                           <  1:543267/100‑1 (MQ=255)
                                        aaaCCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGcc                                                          >  2:3662180/1‑100 (MQ=255)
                                           ccAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcgac                                                       <  1:2486982/100‑1 (MQ=255)
                                           ccAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcgac                                                       <  1:3707764/100‑1 (MQ=255)
                                           ccAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcgac                                                       <  2:3684505/100‑1 (MQ=255)
                                             aGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcgacga                                                     <  2:3199819/100‑1 (MQ=255)
                                              gcgcCGGGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcgacg                                                      <  1:3011948/98‑1 (MQ=255)
                                                gccgcGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCa                                                  <  1:1039668/100‑1 (MQ=255)
                                                gccgcGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCa                                                  >  1:166438/1‑100 (MQ=255)
                                                  cgcgTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCa                                                  <  1:1115636/98‑1 (MQ=255)
                                                  cgcgTTAAGCACAATAACCTAGATCGGAGCGTTGAGTAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAGGGCGACGATc                                                   <  1:2654112/97‑1 (MQ=255)
                                                       tAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACgg                                           >  2:455630/1‑100 (MQ=255)
                                                         aGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGACGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGAGCAGCAACGGt                                          >  1:3672883/1‑99 (MQ=255)
                                                           cccAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGc                                       <  1:2001464/100‑1 (MQ=255)
                                                             cAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGGATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCa                                      <  1:805302/99‑1 (MQ=255)
                                                              aaTAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAACATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGt                                    <  1:1095816/100‑1 (MQ=255)
                                                               aTAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTg                                   <  2:908145/100‑1 (MQ=255)
                                                                tAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGc                                  >  2:1683458/1‑100 (MQ=255)
                                                                  aCCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGa                                >  2:1252885/1‑100 (MQ=255)
                                                                    cGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACAGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGa                                >  1:256554/1‑98 (MQ=255)
                                                                        agcgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGt                          <  2:945497/100‑1 (MQ=255)
                                                                         gcgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGtt                         >  1:1917631/1‑100 (MQ=255)
                                                                         gcgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGtt                         >  2:1619496/1‑100 (MQ=255)
                                                                         gcgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGt                          <  1:423835/99‑1 (MQ=255)
                                                                          cgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCCCCACCGTCAAGCCTACGATCAGAAAAGGTAGAAGTGCGATCAGGttt                        >  2:297070/1‑100 (MQ=255)
                                                                          cgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGttt                        <  2:196901/100‑1 (MQ=255)
                                                                             agcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTtgt                     <  2:3320106/100‑1 (MQ=255)
                                                                              gcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTtgtg                    <  2:2662862/100‑1 (MQ=255)
                                                                              gcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACAACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTtgtg                    >  2:3409944/1‑100 (MQ=255)
                                                                               cgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTgg                   <  2:277655/100‑1 (MQ=255)
                                                                               cgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTgg                   <  2:3226094/100‑1 (MQ=255)
                                                                                gtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGa                  <  1:2620168/100‑1 (MQ=255)
                                                                                gtTGAGGAAAGGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGa                  >  1:2375820/1‑100 (MQ=255)
                                                                                 ttGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAg                 <  2:3191612/100‑1 (MQ=255)
                                                                                  tGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGt                <  1:1593282/100‑1 (MQ=255)
                                                                                     ggAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCGGTGCGATCCGGTTTTGTGGAGTTAt             >  2:3280340/1‑100 (MQ=255)
                                                                                       tctccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTg           >  2:2887766/6‑100 (MQ=255)
                                                                                        ctccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGt          >  1:1343447/5‑100 (MQ=255)
                                                                                        aaTGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGt          <  2:3223292/100‑1 (MQ=255)
                                                                                         aTGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTg           <  2:1652309/98‑1 (MQ=255)
                                                                                         aTGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTc         <  1:2911685/100‑1 (MQ=255)
                                                                                          tGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa        >  2:3794770/1‑100 (MQ=255)
                                                                                          tGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa        >  1:169501/1‑100 (MQ=255)
                                                                                          tGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATGGTCa        >  2:2149074/1‑100 (MQ=255)
                                                                                          ccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTCGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa        >  2:3376194/3‑100 (MQ=255)
                                                                                          ccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa        >  2:2693242/3‑100 (MQ=255)
                                                                                          acaTTGCGTGCCGACGCAATGGAGAGCATCACAACAACCACCGTCAAGCCGAAGATCAGCAACGGTAGAAGTGCGAGCAGGATTTGTGGAGTTATTGTCa        >  1:91234/3‑100 (MQ=255)
                                                                                           caTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCCAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCAt       >  1:1790666/2‑100 (MQ=255)
                                                                                               gCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCg   >  2:3484274/1‑100 (MQ=255)
                                                                                               gCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCg   <  1:1405885/100‑1 (MQ=255)
                                                                                                cGTCGCCACGCAATGGAGAGCATCACAACCACAACCGTCCAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGa  <  1:2680232/100‑1 (MQ=255)
                                                                                                  tCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGAGCAGCAACGGTAGCAGCGCGATCAGGCTTTGTGGAGTTATTGTCATGGcac  >  1:1076378/1‑96 (MQ=255)
                                                                                                   |                                                                                                
GTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGA  >  NC_000913/2391315‑2391510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: