Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,391,417 C→G 16.6% W30S (TGG→TCG)  nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,391,4170CG16.6% 309.2 / 52.7 120W30S (TGG→TCG) nuoNNADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base C (49/51);  new base G (7/13);  total (56/64)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.00e-01

Only 100 of 124 total aligned reads displayed.

ACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACG  >  NC_000913/2391318‑2391516
                                                                                                   |                                                                                                   
aCGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGcc                                                                                                     <  2:306030/100‑1 (MQ=255)
  gTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCAc                                                                                                   >  1:2485802/1‑100 (MQ=255)
  gTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCAc                                                                                                   >  1:34305/1‑100 (MQ=255)
     cacaGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGGGGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGc                                                                                                 <  1:544966/96‑1 (MQ=255)
     cATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCa                                                                                                >  2:1167607/1‑100 (MQ=255)
     cATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCa                                                                                                >  2:1681827/1‑100 (MQ=255)
     cATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCa                                                                                                >  2:727874/1‑100 (MQ=255)
     cATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCa                                                                                                <  1:1741580/100‑1 (MQ=255)
         gcgcCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtca                                                                                            <  1:2964893/100‑3 (MQ=255)
         gcgcCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATgg                                                                                            >  1:2972875/1‑100 (MQ=255)
         gcgcCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATgg                                                                                            >  1:2195091/1‑100 (MQ=255)
          cgcccgccTGGCCAACAAACCAGGGCGAAACCAGCGCCGCGTTAAGCCCCATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtca                                                                                            <  1:454148/99‑3 (MQ=255)
          cgcccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcat                                                                                           <  2:892595/100‑4 (MQ=255)
          cgcccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcat                                                                                           <  2:1114740/100‑4 (MQ=255)
          cgcccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcat                                                                                           <  2:3289451/100‑4 (MQ=255)
          cgcccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGga                                                                                           >  2:3005588/1‑100 (MQ=255)
           gcccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatt                                                                                          <  1:3666221/100‑5 (MQ=255)
           gcccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGgag                                                                                          <  1:995643/100‑1 (MQ=255)
             ccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGagag                                                                                        <  2:2149427/100‑1 (MQ=255)
              cgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGc                                                                                       >  2:2023823/1‑100 (MQ=255)
               gccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttcct                                                                                      <  1:2160650/100‑9 (MQ=38)
               gccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCa                                                                                      <  1:530299/100‑1 (MQ=255)
                ccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttcctc                                                                                     <  1:2113695/100‑10 (MQ=38)
                 cTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttcc                                                                                       <  1:2818086/97‑8 (MQ=38)
                 cTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATc                                                                                    <  1:3316920/100‑1 (MQ=255)
                     ccAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACaa                                                                                >  1:1874265/1‑100 (MQ=255)
                      caacaaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTCGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACaa                                                                                >  1:2487952/1‑99 (MQ=255)
                       aacaaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAacc                                                                              <  2:2282854/100‑1 (MQ=255)
                           aaCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAaccacca                                                                          >  1:1306400/1‑100 (MQ=255)
                           aaCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGGTGAGGAAATGATTGCGTCGCCACGCAACGGAGAGCATCACAaccacca                                                                          >  1:2476421/1‑100 (MQ=255)
                            aCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGGAATGAGTGCGTCGCCCCGCAAAGGAGAGCATCACAaccaccac                                                                         >  1:223174/1‑100 (MQ=255)
                            aCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAaccaccac                                                                         >  2:536670/1‑100 (MQ=255)
                              cAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCg                                                                       <  2:2934306/100‑1 (MQ=255)
                              cAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCg                                                                       <  1:553167/100‑1 (MQ=255)
                              cAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCg                                                                       <  2:117813/100‑1 (MQ=255)
                                gagCGAAACCAGCGCCGTGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAAGCACCACCGTc                                                                     >  1:2506539/1‑100 (MQ=255)
                                gagCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTc                                                                     <  2:1525536/100‑1 (MQ=255)
                                   cGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGTGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAg                                                                  <  1:2586279/100‑1 (MQ=255)
                                    gAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGc                                                                 <  1:543267/100‑1 (MQ=255)
                                    gAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGc                                                                 <  1:2548834/100‑1 (MQ=255)
                                     aaaCCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGcc                                                                >  2:3662180/1‑100 (MQ=255)
                                        ccAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcgac                                                             <  2:3684505/100‑1 (MQ=255)
                                        ccAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcgac                                                             <  1:3707764/100‑1 (MQ=255)
                                          aGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcgacga                                                           <  2:3199819/100‑1 (MQ=255)
                                           gcgcCGGGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcgacg                                                            <  1:3011948/98‑1 (MQ=255)
                                             gccgcGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCa                                                        <  1:3296101/100‑1 (MQ=255)
                                             gccgcGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCa                                                        >  1:166438/1‑100 (MQ=255)
                                             gccgcGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCa                                                        <  1:1039668/100‑1 (MQ=255)
                                               cgcgTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCa                                                        <  1:1115636/98‑1 (MQ=255)
                                               cgcgTTAAGCACAATAACCTAGATCGGAGCGTTGAGTAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAGGGCGACGATc                                                         <  1:2654112/97‑1 (MQ=255)
                                                    tAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACgg                                                 >  2:455630/1‑100 (MQ=255)
                                                        cccAATAACCGAGAGCGTCGCGTTGAGGAAATGACTGCGCCGCCACGCACTGGAGAGCAGCACACCCACCACCGTCACGCCGACGATAAGCAACGCTAg                                              >  1:1620110/1‑99 (MQ=255)
                                                        cccAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGc                                             <  1:2001464/100‑1 (MQ=255)
                                                         ccAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGc                                             <  2:3439253/99‑1 (MQ=255)
                                                          cAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGGATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCa                                            <  1:805302/99‑1 (MQ=255)
                                                           aaTAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAACATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGt                                          <  1:1095816/100‑1 (MQ=255)
                                                            aTAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTg                                         <  2:908145/100‑1 (MQ=255)
                                                             tAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGc                                        >  2:1683458/1‑100 (MQ=255)
                                                              aaCCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCg                                       <  2:3197374/100‑1 (MQ=255)
                                                               aCCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGa                                      >  2:1252885/1‑100 (MQ=255)
                                                               aCCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGa                                      >  2:20433/1‑100 (MQ=255)
                                                                 cGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACAGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGa                                      >  1:256554/1‑98 (MQ=255)
                                                                  gagaGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCa                                   <  2:2342817/100‑1 (MQ=255)
                                                                     agcgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGt                                <  2:945497/100‑1 (MQ=255)
                                                                      gcgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGtt                               >  2:1619496/1‑100 (MQ=255)
                                                                      gcgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGtt                               >  1:1917631/1‑100 (MQ=255)
                                                                      gcgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGt                                <  1:423835/99‑1 (MQ=255)
                                                                       cgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCCCCACCGTCAAGCCTACGATCAGAAAAGGTAGAAGTGCGATCAGGttt                              >  2:297070/1‑100 (MQ=255)
                                                                       cgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGttt                              <  2:196901/100‑1 (MQ=255)
                                                                        gtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGtttt                             >  2:3815063/1‑100 (MQ=255)
                                                                          agcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTtgt                           <  2:3320106/100‑1 (MQ=255)
                                                                           gcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTtgtg                          <  2:2662862/100‑1 (MQ=255)
                                                                            cgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTgg                         <  2:277655/100‑1 (MQ=255)
                                                                             gtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGa                        <  1:2620168/100‑1 (MQ=255)
                                                                             gtTGAGGAAAGGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGa                        >  1:2375820/1‑100 (MQ=255)
                                                                              ttGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAg                       <  2:3191612/100‑1 (MQ=255)
                                                                               tGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGt                      <  1:20432/100‑1 (MQ=255)
                                                                               tGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGt                      <  1:1593282/100‑1 (MQ=255)
                                                                                gAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGtt                     >  1:2199839/1‑100 (MQ=255)
                                                                                  ggAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCGGTGCGATCCGGTTTTGTGGAGTTAt                   >  2:3280340/1‑100 (MQ=255)
                                                                                  ggAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTa                    >  1:995094/1‑99 (MQ=255)
                                                                                  ggAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACAACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGGGCGAGCAGGTTTTGTGGAGTTa                    >  1:2384262/1‑99 (MQ=255)
                                                                                    tctccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTg                 >  2:2887766/6‑100 (MQ=255)
                                                                                     aaTGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGt                <  2:3223292/100‑1 (MQ=255)
                                                                                      aTGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTg                 <  2:1652309/98‑1 (MQ=255)
                                                                                      aTGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTc               <  1:2911685/100‑1 (MQ=255)
                                                                                       tGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa              <  1:3676661/100‑1 (MQ=255)
                                                                                       tGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa              >  1:169501/1‑100 (MQ=255)
                                                                                       tGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa              <  2:116138/100‑1 (MQ=255)
                                                                                       ccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCTCCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa              >  2:2692506/3‑100 (MQ=255)
                                                                                       ccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTCGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa              >  2:3376194/3‑100 (MQ=255)
                                                                                       ccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa              >  2:2693242/3‑100 (MQ=255)
                                                                                       acaTTGCGTGCCGACGCAATGGAGAGCATCACAACAACCACCGTCAAGCCGAAGATCAGCAACGGTAGAAGTGCGAGCAGGATTTGTGGAGTTATTGTCa              >  1:91234/3‑100 (MQ=255)
                                                                                        caTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCCAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCAt             >  1:1790666/2‑100 (MQ=255)
                                                                                            gCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCg         >  2:3484274/1‑100 (MQ=255)
                                                                                            gCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCg         <  1:1405885/100‑1 (MQ=255)
                                                                                             cGTCGCCACGCAATGGAGAGCATCACAACCACAACCGTCCAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGa        <  1:2680232/100‑1 (MQ=255)
                                                                                               tCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGAGCAGCAACGGTAGCAGCGCGATCAGGCTTTGTGGAGTTATTGTCATGGcac        >  1:1076378/1‑96 (MQ=255)
                                                                                                 gCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTa    >  2:889805/1‑100 (MQ=255)
                                                                                                   cACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACg  <  2:2171556/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACG  >  NC_000913/2391318‑2391516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: