Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,707,321 A→T 15.6% intergenic (‑197/+1) lepA ← / ← rseC back‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,707,3210AT15.6% 460.6 / 65.9 178intergenic (‑197/+1)lepA/rseCback‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component
Reads supporting (aligned to +/- strand):  ref base A (84/66);  new base T (11/17);  total (95/83)
Fisher's exact test for biased strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 182 total aligned reads displayed.

CACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAA  >  NC_000913/2707222‑2707420
                                                                                                   |                                                                                                   
nactacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGaaa                                                                                                     >  2:1196968/2‑100 (MQ=255)
  ctacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATc                                                                                                   >  2:2941968/1‑100 (MQ=255)
  ctacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATc                                                                                                   >  1:3211849/1‑100 (MQ=255)
  ctacaACATTGAGGCGAGGAAATGTTAATACCGTATGGATTGTGGTATGTGGAAACGTCCTCGCATTTGTTATGAAAAATGCAACAAAGCCAGTGAAATc                                                                                                   >  2:689987/1‑100 (MQ=255)
     caacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACt                                                                                                <  1:1296357/100‑1 (MQ=255)
         aTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCt                                                                                            >  2:1758825/1‑100 (MQ=255)
         aTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCt                                                                                            >  2:177511/1‑100 (MQ=255)
          ttGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtt                                                                                           <  1:1758816/100‑2 (MQ=255)
          ttGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtt                                                                                           <  2:3422002/100‑2 (MQ=255)
          ttGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCt                                                                                            <  2:787902/99‑1 (MQ=255)
          ttGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGc                                                                                             <  1:3640832/98‑1 (MQ=255)
           tGAGTCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCttt                                                                                          <  1:3189314/100‑3 (MQ=255)
             aGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTcgcg                                                                                        <  1:418688/100‑1 (MQ=255)
             aGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTcgcg                                                                                        <  2:2163025/100‑1 (MQ=255)
             aGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTcgcg                                                                                        >  2:2023818/1‑100 (MQ=255)
             aGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTcgcg                                                                                        <  1:775021/100‑1 (MQ=255)
              ggCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAACCAGTGAAATCACTGGCTcgcg                                                                                        >  1:1108393/1‑99 (MQ=255)
               gCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGt                                                                                       >  1:483337/1‑99 (MQ=255)
               gCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTc                                                                                      >  2:1557485/1‑100 (MQ=255)
               gCGAGGAAATGTTCATACCGTATGGACTGTGGTATCTGGAAACGTCCTCGCATTTGTGATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttg                                                                                         <  1:2487338/97‑4 (MQ=255)
                cGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACATCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCt                                                                                     >  2:2582505/1‑100 (MQ=255)
                cGAAGAGATGGTCATAACGTATGTTTTGTCGTATCTAAAAACGTTCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgttg                                                                                      <  1:1029194/99‑7 (MQ=19)
                  aGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTc                                                                                   <  2:2752281/100‑1 (MQ=255)
                   ggAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTcc                                                                                  >  2:3797613/1‑100 (MQ=255)
                      aaTGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGaa                                                                               >  2:190436/1‑100 (MQ=255)
                      aaTGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGaa                                                                               <  1:2016646/100‑1 (MQ=255)
                      aaTGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGaa                                                                               <  2:3370522/100‑1 (MQ=255)
                       aTGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAg                                                                              <  2:3746496/100‑1 (MQ=255)
                        tGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGa                                                                             >  1:92334/1‑100 (MQ=255)
                        tGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGa                                                                             <  1:807244/100‑1 (MQ=255)
                         gTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGAt                                                                            >  1:1007611/1‑100 (MQ=255)
                         gTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCCTCTTCCGAAGAt                                                                            >  1:228719/1‑100 (MQ=255)
                            cATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGtt                                                                         >  1:508727/1‑100 (MQ=255)
                            cATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGtt                                                                         >  2:3071269/1‑100 (MQ=255)
                               aCCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCa                                                                      <  2:1727151/100‑1 (MQ=255)
                                  gTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAAt                                                                   >  2:874940/1‑100 (MQ=255)
                                     tGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGc                                                                <  2:2634594/100‑1 (MQ=255)
                                      ggATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGca                                                               <  2:2223166/100‑1 (MQ=255)
                                      ggATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGca                                                               >  1:1985549/1‑100 (MQ=255)
                                       gATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGcac                                                              >  1:981767/1‑100 (MQ=255)
                                         ttGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACaa                                                            >  1:3096612/1‑100 (MQ=255)
                                             ggTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGcc                                                        >  1:1312785/1‑100 (MQ=255)
                                               tATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCAGAAGATCTTTCAAATCGCACAAGGCCAg                                                      >  1:3468282/1‑100 (MQ=255)
                                                aTCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAgg                                                     >  2:581965/1‑100 (MQ=255)
                                                  cTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCGTCCGAAGATGTTTCAAATCGCACAAGGCCAggc                                                    >  1:3805544/1‑99 (MQ=255)
                                                   tGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAggcgg                                                  >  2:4721/1‑100 (MQ=255)
                                                   tGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAggcgg                                                  >  2:17975/1‑100 (MQ=255)
                                                    ggAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGAGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAggcgg                                                  >  1:923334/1‑99 (MQ=255)
                                                         cGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGc                                            <  1:2481636/100‑1 (MQ=255)
                                                          gTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGcc                                           <  2:2308220/100‑1 (MQ=255)
                                                          gTCCTCGCATTCGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGcc                                           >  1:2927671/1‑100 (MQ=255)
                                                            ccTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCAc                                         <  2:3549862/100‑1 (MQ=255)
                                                            ccTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCAc                                         <  1:887441/100‑1 (MQ=255)
                                                             cTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACg                                        >  1:2938535/1‑100 (MQ=255)
                                                             cTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACg                                        >  2:511132/1‑100 (MQ=255)
                                                             cTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACg                                        >  2:1221398/1‑100 (MQ=255)
                                                               cGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCt                                      <  2:1140803/100‑1 (MQ=255)
                                                               cGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCt                                      >  1:2159727/1‑100 (MQ=255)
                                                                 cATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTa                                    >  2:1452792/1‑100 (MQ=255)
                                                                  aTTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGACTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATCCCACGCTTaa                                   >  1:2758897/1‑100 (MQ=255)
                                                                   tttGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAg                                  <  2:3386059/100‑1 (MQ=255)
                                                                    ttGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAga                                 <  2:31805/100‑1 (MQ=255)
                                                                    ttGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAACGCCACGCTTAAga                                 <  1:2350959/100‑1 (MQ=255)
                                                                      gTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAgatg                               <  1:1874392/100‑1 (MQ=255)
                                                                      gTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCCTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAgat                                <  2:2696214/99‑1 (MQ=255)
                                                                       ttATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAgatga                              >  2:1100137/1‑100 (MQ=255)
                                                                        tATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAgatgat                             >  1:3664574/1‑100 (MQ=255)
                                                                         aTGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAgatgat                             >  1:2783655/1‑99 (MQ=255)
                                                                         aTGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATc                            >  1:828781/1‑100 (MQ=255)
                                                                          tGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCg                           >  2:3017306/1‑100 (MQ=255)
                                                                           gCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCgg                          <  1:189257/100‑1 (MQ=255)
                                                                           gCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCgg                          >  2:3311194/1‑100 (MQ=255)
                                                                            cAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGt                         >  2:3696398/1‑100 (MQ=255)
                                                                            cAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGt                         >  1:433045/1‑100 (MQ=255)
                                                                            cAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGt                         <  2:3480124/100‑1 (MQ=255)
                                                                             aaaaTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTGAAATCGCACACGGCCAGGCGGCAATGCCACGCTTACGATGATCg                           >  1:3676342/1‑97 (MQ=255)
                                                                                aTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGcc                     >  2:963118/1‑100 (MQ=255)
                                                                                  gCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCAt                   <  1:2789341/100‑1 (MQ=255)
                                                                                   gacgcgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCGCCGCTTAAGATGATCGGTTGCCAt                   >  1:2206079/7‑99 (MQ=38)
                                                                                   caacaaAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCCCGCTTAAGATGATCGGTTGcc                     >  1:3483732/1‑97 (MQ=255)
                                                                                    aacaaAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTc                 <  1:1507333/100‑1 (MQ=255)
                                                                                    aacaaAGCCAGTGAAAGCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTc                 <  2:3010409/100‑1 (MQ=255)
                                                                                     cgcgaGCCAGTGATTTCACTGGCTAGCATCTACCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAAGCCGACGCTTAACATGAACGGTTGCCATTCt                >  1:1622091/5‑100 (MQ=255)
                                                                                       cgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAATTCGCACAAGGCCAGCCGGCCATGCCGCGCGTAAGATGATCCGTTGCCATTCTGc              >  1:302119/3‑100 (MQ=255)
                                                                                       cgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCGGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGc              >  1:1775388/3‑100 (MQ=255)
                                                                                       cgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGc              >  2:2371798/3‑100 (MQ=255)
                                                                                        gaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGcc             >  2:2993155/2‑100 (MQ=255)
                                                                                        gaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGcc             >  1:3390001/2‑100 (MQ=255)
                                                                                        gaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGcc             >  1:2686971/2‑100 (MQ=255)
                                                                                         aGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGccc            <  2:906336/100‑1 (MQ=255)
                                                                                          gCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCg           <  1:2066194/100‑1 (MQ=255)
                                                                                           cgaGTGACAACACTGCCTCGGGACTTCGGCAGATGTTTCAAAGCGCACAAGGCCAGGCGGCAATGCCACGCTTAATACTATTTGGTGCCATTCTGCCCgg          <  1:2631445/98‑1 (MQ=255)
                                                                                           ccAGTGATTTCACTGGCTTGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCgg          >  1:108222/1‑100 (MQ=255)
                                                                                            cAGTGAAATCACTAGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCggg         >  2:2018225/1‑100 (MQ=255)
                                                                                             aGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGgc        <  2:1312793/100‑1 (MQ=255)
                                                                                              gTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGgca       <  1:1019549/100‑1 (MQ=255)
                                                                                               tGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGgcag      <  2:1985559/100‑1 (MQ=255)
                                                                                               tGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATACCAAGCTTAAGATGATCGGTTGACATTCTGCCTGGgca       >  1:1931002/1‑99 (MQ=255)
                                                                                                gAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGgcagc     >  2:3655945/1‑100 (MQ=255)
                                                                                                   aTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCTCAAGGCCAGGCGGCAATGCTACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCaaa  <  1:2074300/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAA  >  NC_000913/2707222‑2707420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: