Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,731,073 A→T 15.0% noncoding (85/1542 nt) rrsG ← 16S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,731,0730AT15.0% 384.8 / 52.4 151noncoding (85/1542 nt)rrsG16S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base A (70/58);  new base T (13/10);  total (83/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-01

Only 100 of 164 total aligned reads displayed.

CATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTC  >  NC_000913/2730974‑2731172
                                                                                                   |                                                                                                   
cATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAgcagca                                                                                                     >  2:155314/1‑100 (MQ=40)
 aTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCaa                                                                                                    <  2:1867760/100‑1 (MQ=40)
  ttATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAg                                                                                                   <  1:3810720/100‑1 (MQ=38)
  ttATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAg                                                                                                   <  1:1555759/100‑1 (MQ=38)
   tATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgc                                                                                                  <  2:2885058/100‑1 (MQ=38)
   tATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgc                                                                                                  >  2:323627/1‑100 (MQ=38)
   tATGCGGTATTAGCTACCGTTCCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgc                                                                                                  <  1:3374166/100‑1 (MQ=255)
        ggTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCtt                                                                                             <  1:77223/100‑1 (MQ=255)
         gTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCTTCAGGCAGCTTCCCAGACATTACGCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTc                                                                                            <  1:2166995/100‑1 (MQ=255)
          tATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTcc                                                                                           <  2:772438/100‑1 (MQ=255)
           aTTAGCTGCCGTTTGCAGTAGTTAGCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCCCCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgc                                                                                          <  1:3085089/100‑3 (MQ=14)
           aTTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgc                                                                                          <  1:1967199/100‑3 (MQ=255)
            ttAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgct                                                                                         <  2:3737478/100‑4 (MQ=14)
            ttAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgct                                                                                         <  1:1626582/100‑4 (MQ=14)
              aGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGtt                                                                                       >  2:2216900/1‑100 (MQ=255)
               gCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTa                                                                                      <  2:832113/100‑1 (MQ=255)
                cTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTAc                                                                                     <  1:2052855/100‑1 (MQ=40)
                cTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTAc                                                                                     >  2:2277591/1‑100 (MQ=255)
                  aCCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCg                                                                                   >  1:2373258/1‑100 (MQ=255)
                  aCCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAACGAAGCAGCAAgct                                                                                                 >  1:1171103/1‑86 (MQ=37)
                     gTTTCCAGTAGTTATCCCCCTCTATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTc                                                                                <  1:947874/100‑1 (MQ=255)
                     gTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTc                                                                                <  2:3487532/100‑1 (MQ=255)
                      tttCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCg                                                                               <  2:3032689/100‑1 (MQ=40)
                      tttCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCg                                                                               <  2:3190332/100‑1 (MQ=255)
                       ttCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGa                                                                              >  2:1064833/1‑100 (MQ=255)
                         ccAGTAGTTGTCCCCCTCCATCAGGCACCTTCCCAGACATTCCTCGCCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTc                                                                                <  1:3583984/96‑1 (MQ=14)
                          cAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACtt                                                                           >  2:1230555/1‑100 (MQ=255)
                          cAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACtt                                                                           >  1:2638509/1‑100 (MQ=255)
                            gtagtTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGc                                                                         <  1:3256301/100‑1 (MQ=255)
                             tagtTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCa                                                                        >  2:3464995/1‑100 (MQ=255)
                             tagtTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCGGCTTCCTGTTACCGTTCGACTTGCa                                                                        <  2:1089543/100‑1 (MQ=255)
                              agtTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCAt                                                                       >  2:218471/1‑100 (MQ=255)
                               gtTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCAtg                                                                      >  2:3387326/1‑100 (MQ=255)
                               gtTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCAtg                                                                      >  2:888151/1‑100 (MQ=255)
                                ttATCCCCCCCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCAtg                                                                      <  2:560598/99‑1 (MQ=255)
                                 tATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCAtgtg                                                                    >  1:2868850/1‑100 (MQ=255)
                                    cccccTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTa                                                                 >  1:2555924/1‑100 (MQ=255)
                                      cccTCCATCAGGCAGCTTCCCAGACCTTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAgg                                                               <  2:874547/100‑1 (MQ=255)
                                      cccTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAgg                                                               <  1:2303522/100‑1 (MQ=255)
                                       ccTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGc                                                              <  2:3554016/100‑1 (MQ=255)
                                        cTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGcc                                                             <  1:1764610/100‑1 (MQ=40)
                                           cATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTg                                                           >  1:3355922/1‑99 (MQ=255)
                                            aTCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTgcc                                                         <  2:2506043/100‑1 (MQ=255)
                                            aTCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTgcc                                                         >  2:1767773/1‑100 (MQ=255)
                                              cAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTgccgc                                                       >  1:2873737/1‑100 (MQ=255)
                                                ggCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTCCCTGTTACCGTTCGCCTTGCATGTGTTAGGCCTgccgcc                                                      >  1:1388089/1‑99 (MQ=255)
                                                  cAGCTTCCCAGACACTACTCACCCGTCCTCCACCCGTCCGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAg                                                    <  1:3036760/99‑1 (MQ=14)
                                                    gCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGGAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGAGt                                                 >  1:1758476/1‑100 (MQ=255)
                                                    gCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGt                                                 <  1:911804/100‑1 (MQ=38)
                                                     cTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGtt                                                >  2:2204/1‑100 (MQ=40)
                                                      ttCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTc                                               <  1:2726181/100‑1 (MQ=38)
                                                      ttCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTc                                               <  2:1351162/100‑1 (MQ=38)
                                                      ttCCCAGACATTACTCACCCGTCCGCAACTCGTCAGCGAAGCATCAAGCTGCCTCCATTTAACGTTTTAAATCCATATGTTAGGCCTCCCGCCAGCGTTc                                               >  1:1443180/1‑100 (MQ=2)
                                                       tCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCa                                              <  1:1712893/100‑1 (MQ=35)
                                                         ccAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAAt                                            >  2:1405571/1‑100 (MQ=38)
                                                           aGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCt                                          >  1:215414/1‑100 (MQ=38)
                                                            gACATGACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACGTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTg                                         <  1:2542427/100‑1 (MQ=255)
                                                              cagtACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCAAGCGTTCAATc                                           <  1:1592882/93‑1 (MQ=255)
                                                              cATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAg                                       <  2:456648/100‑1 (MQ=38)
                                                                ttACTCACCCGTCCGCCACTCGTCAGCGAATCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTCCCGCCAGCGTTCAATCTGAg                                       <  1:921710/98‑1 (MQ=12)
                                                                ttACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGcc                                     >  2:57326/1‑100 (MQ=38)
                                                                  aCTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCAt                                   >  2:2994734/1‑100 (MQ=38)
                                                                   cTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATg                                  <  2:3510314/100‑1 (MQ=38)
                                                                    tCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGa                                 <  2:1833/100‑1 (MQ=38)
                                                                     cACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGAt                                >  2:3804992/1‑100 (MQ=38)
                                                                     cACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGAt                                >  2:2739074/1‑100 (MQ=38)
                                                                      aCCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCATGTGTTAATCCTGCCTCCAGCGTTCAATCTGAGCCATGATc                               >  1:314802/1‑100 (MQ=11)
                                                                          gTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAAc                           >  2:1988584/1‑100 (MQ=35)
                                                                            ccGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAActc                         >  1:2693084/1‑100 (MQ=38)
                                                                             cGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAActct                        >  2:1987812/1‑100 (MQ=38)
                                                                              gCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCtt                       >  1:372646/1‑100 (MQ=38)
                                                                               ccACTCGTCAGCGAAGCAGCTAGCTGCGTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTc                      <  1:2492119/100‑1 (MQ=255)
                                                                               ccACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTc                      >  1:3000962/1‑100 (MQ=38)
                                                                                cACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCa                     <  1:888144/100‑1 (MQ=38)
                                                                                cACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCa                     >  1:2255573/1‑100 (MQ=38)
                                                                                  cTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAAt                   <  2:1747420/100‑1 (MQ=38)
                                                                                  cTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAAt                   >  2:3360861/1‑100 (MQ=38)
                                                                                    cGTCAGCGAAGCAGAAAGCTAATACCTGTTACCGTTCTACTTGCATGTGTTAGGCCGGCTGCAAGCGATAAATCTGAGCCATGATCAAACTCTTCAAttt                 >  1:3188455/1‑100 (MQ=11)
                                                                                     gTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGCTCAAACTCTTCAATTTa                <  1:3553668/100‑1 (MQ=38)
                                                                                     gTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTa                >  1:1916067/1‑100 (MQ=38)
                                                                                     gTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTa                >  2:1171586/1‑100 (MQ=38)
                                                                                      tCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTaa               >  2:2085974/1‑100 (MQ=38)
                                                                                       caccGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGAATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTaaa              <  1:2961278/97‑1 (MQ=255)
                                                                                       cAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTaaa              >  2:2700071/1‑100 (MQ=38)
                                                                                        caggAAGCAGCTTGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTaaaa             >  2:1627049/4‑100 (MQ=255)
                                                                                         gccaagaagCAAGCTGCTTCCTGTTACTGTTCGACTTGCAGGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAg            >  1:1044419/4‑100 (MQ=1)
                                                                                         gCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAg            <  2:319423/100‑1 (MQ=38)
                                                                                         gCGAAGCAGAAAGCTGCTTCATGTTACCGTTCGACTTGAATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAg            <  1:1171968/100‑1 (MQ=255)
                                                                                          ggAAGCAGCTTGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGt           >  1:2560264/2‑100 (MQ=255)
                                                                                          ggAAGCAGCTTGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGt           >  1:2630886/2‑100 (MQ=255)
                                                                                          ggAAGCAGCTTGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGt           >  1:3548021/2‑100 (MQ=255)
                                                                                          ggAAGCAGCTTGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGACATGATCAAACTCTTCAATTTAAAAGt           >  2:1519138/2‑100 (MQ=255)
                                                                                             agcagcAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTg        >  2:908246/1‑100 (MQ=35)
                                                                                             agcagcAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTg        >  1:876942/1‑100 (MQ=35)
                                                                                              gcagcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGa       <  2:3104304/100‑1 (MQ=35)
                                                                                               cagcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGAt      >  2:3639004/1‑100 (MQ=33)
                                                                                                agctagctGCTTCCGGGTACCGTTTGACTTTCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATg     <  1:1222581/100‑1 (MQ=11)
                                                                                                agcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATg     >  2:1967743/1‑100 (MQ=33)
                                                                                                agcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATg     >  1:1072312/1‑100 (MQ=33)
                                                                                                  caAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCt   <  1:2764992/100‑1 (MQ=32)
                                                                                                   |                                                                                                   
CATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTC  >  NC_000913/2730974‑2731172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: