Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,068,924 G→A 15.3% intergenic (‑116/+23) argO ← / ← mscS arginine transporter/mechanosensitive channel protein, small conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,068,9240GA15.3% 428.2 / 55.1 170intergenic (‑116/+23)argO/mscSarginine transporter/mechanosensitive channel protein, small conductance
Reads supporting (aligned to +/- strand):  ref base G (81/62);  new base A (15/11);  total (96/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

Only 100 of 179 total aligned reads displayed.

AGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCAT  >  NC_000913/3068827‑3069023
                                                                                                 |                                                                                                   
aGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAAATGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGta                                                                                                   >  1:2565937/1‑100 (MQ=255)
  tATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatt                                                                                                 >  2:467263/1‑100 (MQ=255)
  tATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatt                                                                                                 >  2:2682169/1‑100 (MQ=255)
   aTCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGta                                                                                                   <  1:1258206/97‑1 (MQ=255)
     cTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAAAGtattat                                                                                               >  1:3539623/1‑99 (MQ=255)
       gATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCt                                                                                             >  1:850940/1‑99 (MQ=255)
        aTGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTgc                                                                                           <  1:755203/100‑2 (MQ=255)
         tGCAGAGACTATATCGGGGGATTGTTATTAGCGGAAATTGATAATATTAAGTGGCAGTCAGAAAGACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGc                                                                                          <  1:143251/100‑1 (MQ=255)
         tGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGc                                                                                          >  1:3273623/1‑100 (MQ=255)
         tGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGc                                                                                          <  2:1690466/100‑1 (MQ=255)
         tGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGc                                                                                          >  1:1833799/1‑100 (MQ=255)
          gCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGcc                                                                                         <  1:447131/100‑1 (MQ=255)
          gCAGAGACTATATCGCAGGATTGTTATTAGCGGAAAATTATAATATTAATAGGAAATAAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGcc                                                                                         <  1:2085200/100‑1 (MQ=255)
           cAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCt                                                                                        <  2:1360046/100‑1 (MQ=255)
           cAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCt                                                                                        <  2:1983208/100‑1 (MQ=255)
           cAGAGAATATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCt                                                                                        <  1:2816958/100‑1 (MQ=255)
            agagACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGcc                                                                                         <  1:1292098/98‑1 (MQ=255)
            agagACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCt                                                                                        <  1:493559/99‑1 (MQ=255)
             gagaCTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTct                                                                                      <  2:1514197/100‑2 (MQ=38)
             gagaCTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTct                                                                                      <  1:3399590/100‑2 (MQ=39)
              agaCTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCa                                                                                      <  1:606210/99‑1 (MQ=255)
               gaCTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGc                                                                                    <  2:894355/100‑1 (MQ=255)
                aCTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCg                                                                                   <  2:1572373/100‑1 (MQ=255)
                  tataTCGGGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTg                                                                                <  2:2966017/100‑1 (MQ=255)
                  tataTCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGtt                                                                                 <  2:3436777/100‑1 (MQ=255)
                   atatCGCGGGATTGTTATGAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTg                                                                                <  1:3024140/100‑1 (MQ=255)
                    tatCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTctctcttat                                                                               <  2:1551739/100‑9 (MQ=25)
                      tCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGAtt                                                                             >  2:3035313/1‑100 (MQ=255)
                      tCGCGGGATTGTGATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTctctcttatta                                                                             <  2:1751163/100‑11 (MQ=25)
                        gcgGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTAc                                                                           >  1:1630706/1‑100 (MQ=255)
                        gcgGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCAAGAGAGTATTATCTGGCCTCAGCGTTGATTAc                                                                           >  1:159630/1‑100 (MQ=255)
                         cgGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAACCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACg                                                                          >  1:759794/1‑100 (MQ=255)
                         cgGGATTGTTATTAGCGGAAAATGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATATGGCCTCAGCGTTGATTACg                                                                          >  1:1487186/1‑100 (MQ=255)
                             aTTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGc                                                                      <  1:1177001/100‑1 (MQ=255)
                                gTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCttt                                                                   >  1:1614625/1‑100 (MQ=255)
                                  tattaGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGt                                                                 >  1:1009573/1‑100 (MQ=255)
                                  tattaGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGt                                                                 <  2:2542280/100‑1 (MQ=255)
                                      aGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtcttc                                                             >  1:414199/1‑100 (MQ=255)
                                        cGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTcttctt                                                           >  1:1772572/1‑100 (MQ=255)
                                        cGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTcttctt                                                           <  2:1055337/100‑1 (MQ=255)
                                          gAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTc                                                         <  2:597/100‑1 (MQ=255)
                                             aTTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCAcc                                                      <  1:1517556/100‑1 (MQ=255)
                                             aTTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCAcc                                                      <  1:1222705/100‑1 (MQ=255)
                                                gATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGc                                                   >  2:2400979/1‑100 (MQ=255)
                                                 ataataTTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGcn                                                  <  1:3286470/100‑2 (MQ=255)
                                                     tatTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTaaa                                              >  2:549038/1‑100 (MQ=255)
                                                     tatTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTaaa                                              >  1:100304/1‑100 (MQ=255)
                                                     tatTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTaaa                                              >  1:3071699/1‑100 (MQ=255)
                                                       ttAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGt                                            <  2:2274844/100‑1 (MQ=255)
                                                        tAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGtt                                           >  1:2841471/1‑100 (MQ=255)
                                                        tAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGtt                                           <  1:2063052/100‑1 (MQ=255)
                                                         acatGGCAATAAGCAAATCTACTAAGAGAGGGGCCACAGAGTATTTTCTGGCGTCAGCGTTGAGTACGCAGCTTTGTCTTCTTTCGCCAGCTTAAAGTTc                                          <  1:680597/97‑1 (MQ=255)
                                                         aaTTGGCAATCAGAAAAACTAATAAGAGAGAGGACAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGATTTGTCTTCTTTCACCCGCTTAAAg                                             >  1:2172099/1‑97 (MQ=255)
                                                          aTTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTca                                         <  2:3348441/100‑1 (MQ=255)
                                                           ttGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTcac                                        <  2:772535/100‑1 (MQ=255)
                                                           ttGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTcac                                        >  1:346205/1‑100 (MQ=255)
                                                            tGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTcaca                                       >  2:812627/1‑100 (MQ=255)
                                                            tGGCAATCAGAAAAACTAATAACAGAGAGTCCAGAGAGTATTATCTTGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTcaca                                       <  1:2587127/100‑1 (MQ=255)
                                                              gCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATc                                     >  1:2848787/1‑100 (MQ=255)
                                                                   cAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTCAAGTTCACATCCAttt                                >  2:1437500/1‑100 (MQ=255)
                                                                    aGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTg                               >  2:22142/1‑100 (MQ=255)
                                                                    aGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTg                               >  2:708805/1‑100 (MQ=255)
                                                                    aGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTg                               >  2:2889976/1‑100 (MQ=255)
                                                                     gAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGc                              >  2:3640794/1‑100 (MQ=255)
                                                                     gAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGc                              >  2:3402982/1‑100 (MQ=255)
                                                                     gAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGc                              >  1:3701019/1‑100 (MQ=255)
                                                                     gAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGc                              >  2:2663706/1‑100 (MQ=255)
                                                                      aaaaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGc                              <  1:1129311/99‑1 (MQ=255)
                                                                      aaaaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCg                             >  2:2187050/1‑100 (MQ=255)
                                                                       aaaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCgg                            <  1:3659048/100‑1 (MQ=255)
                                                                       aaaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCgg                            >  2:119582/1‑100 (MQ=255)
                                                                       aaaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCgg                            <  1:2975142/100‑1 (MQ=255)
                                                                          aCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTa                         <  1:2400967/100‑1 (MQ=255)
                                                                           cTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCATCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGTTa                         >  1:190753/1‑99 (MQ=255)
                                                                           cTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTAc                        >  2:351148/1‑100 (MQ=255)
                                                                           cTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTAc                        >  2:2215816/1‑100 (MQ=255)
                                                                                 acgctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAg                  >  2:2274209/6‑100 (MQ=37)
                                                                                 acgctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAg                  >  2:3119669/6‑100 (MQ=37)
                                                                                 acgctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAg                  >  2:141000/6‑100 (MQ=37)
                                                                                   gctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCt                >  1:3681000/4‑100 (MQ=38)
                                                                                   gctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCt                >  1:3686587/4‑100 (MQ=38)
                                                                                   gctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCt                >  2:2733989/4‑100 (MQ=38)
                                                                                   gctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCt                >  2:641397/4‑100 (MQ=38)
                                                                                   agagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCt                <  1:2373865/100‑1 (MQ=255)
                                                                                   agagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCt                <  1:3209500/100‑1 (MQ=255)
                                                                                   agagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCt                <  1:324959/100‑1 (MQ=255)
                                                                                    ctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTg               >  1:519312/3‑100 (MQ=38)
                                                                                    ctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTg               >  2:1931988/3‑100 (MQ=38)
                                                                                    ctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTg               >  2:1598424/3‑100 (MQ=38)
                                                                                      gagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGAt             >  2:430495/1‑100 (MQ=255)
                                                                                       agGCCAGAGAGTATTATCTTGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATa            <  1:3598405/100‑1 (MQ=255)
                                                                                        ggCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAc           >  1:3810961/1‑100 (MQ=255)
                                                                                         gCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAc           <  2:3701042/99‑1 (MQ=255)
                                                                                         gCCAGAGAGTATTATCCGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAcc          <  1:2227944/100‑1 (MQ=255)
                                                                                          ccAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACcg         >  1:2790521/1‑100 (MQ=255)
                                                                                             gagagTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACcggcg      <  2:748217/100‑1 (MQ=255)
                                                                                              agagTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACcggcgg     <  2:3000016/100‑1 (MQ=255)
                                                                                               gagTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCggcggc    <  1:2615289/100‑1 (MQ=255)
                                                                                               gagTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCggcggc    <  1:2479073/100‑1 (MQ=255)
                                                                                                 gTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCAt  >  2:2456543/1‑100 (MQ=255)
                                                                                                 |                                                                                                   
AGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCAT  >  NC_000913/3068827‑3069023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: