BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,566,544	C→A	100%	S13I (AGC→ATC)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,566,544	0	C	A	98.6%	472.2 / ‑1.7	141	S13I (AGC→ATC)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand): ref base C (2/0); new base A (70/69); total (72/69)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

Only 100 of 141 total aligned reads displayed.

TGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCG  >  NC_000913/3566450‑3566640

 gCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACCTCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTCCTACTATAAGCg                                                                                            <  1:2968511/100‑1 (MQ=255)

   gTTCAGCCATTGATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTGGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTg                                                                                          >  1:3039000/1‑100 (MQ=255)

   gTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTg                                                                                          >  2:3655467/1‑100 (MQ=255)

    ttCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTgg                                                                                         <  1:2812398/100‑1 (MQ=255)

      cAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGc                                                                                       >  2:249363/1‑100 (MQ=255)

      cAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGc                                                                                       >  1:672815/1‑100 (MQ=255)

       aGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCg                                                                                      <  1:1182917/100‑1 (MQ=255)

       aGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCg                                                                                      <  1:2252306/100‑1 (MQ=255)

        gCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCACTCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGa                                                                                     >  2:2627750/1‑100 (MQ=255)

        gCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGa                                                                                     >  2:1624080/1‑100 (MQ=255)

        gCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTATAAGCGATAGAGGGCTTAAGAGCTTCTACGATAAGCGTGGGCGa                                                                                     >  2:226786/1‑100 (MQ=255)

         ccATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGAt                                                                                    <  1:464540/100‑1 (MQ=255)

            ttCATGTTTATTGGCGACGACCGGGTACTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGaa                                                                                 <  1:2921801/100‑1 (MQ=255)

             tCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGaa                                                                                 <  1:538587/99‑1 (MQ=255)

              cATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAata                                                                               <  2:170610/100‑1 (MQ=255)

                tGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATg                                                                             >  2:1120914/1‑100 (MQ=255)

                tGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATg                                                                             <  1:216280/100‑1 (MQ=255)

                tGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATg                                                                             <  2:421631/100‑1 (MQ=255)

                  tttATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTa                                                                           <  2:2312383/100‑1 (MQ=255)

                   ttATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTaa                                                                          >  1:3675835/1‑100 (MQ=255)

                   ttATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTaa                                                                          <  2:1829970/100‑1 (MQ=255)

                   ttATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATAAATATGTaa                                                                          >  1:1734952/1‑100 (MQ=255)

                    tATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTaaa                                                                         >  2:348566/1‑100 (MQ=255)

                     aTTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAAc                                                                        >  2:1803169/1‑100 (MQ=255)

                      ttGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACg                                                                       <  2:3536016/100‑1 (MQ=255)

                      ttGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACg                                                                       <  1:662617/100‑1 (MQ=255)

                        ggCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAATCGTGGGCGATGAATATGTAAACGGa                                                                     >  2:3456765/1‑100 (MQ=255)

                        ggCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGa                                                                     >  2:3590562/1‑100 (MQ=255)

                         gCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAg                                                                    <  2:177201/100‑1 (MQ=255)

                          cgacgaCCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGc                                                                   >  2:3334160/1‑100 (MQ=255)

                          cgacgaCCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGc                                                                   >  2:3774229/1‑100 (MQ=255)

                           gacgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCa                                                                  >  2:879045/1‑100 (MQ=255)

                             cgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCAtt                                                                >  1:2278975/1‑100 (MQ=255)

                             cgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCAtt                                                                >  1:3319623/1‑100 (MQ=255)

                             cgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCAt                                                                 >  1:3319428/1‑99 (MQ=255)

                                ccGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCat                                                             >  2:2412038/1‑100 (MQ=255)

                                ccGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCat                                                             <  2:1122351/100‑1 (MQ=255)

                                ccGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCat                                                             >  1:1175874/1‑100 (MQ=255)

                                 cGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCa                                                              <  1:1863481/98‑1 (MQ=255)

                                  gggTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCatat                                                           >  1:815615/1‑100 (MQ=255)

                                     tCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAgg                                                        <  1:2871273/100‑1 (MQ=255)

                                      ccTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGc                                                       <  2:2892176/100‑1 (MQ=255)

                                      ccTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGc                                                       <  1:1917827/100‑1 (MQ=255)

                                       cTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCg                                                      <  1:842305/100‑1 (MQ=255)

                                         ttCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGtt                                                    <  2:1565495/100‑1 (MQ=255)

                                            cAATCGTAAACATCTGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGCATATGTAAACGGAGCATTCATATAGGCGTTTca                                                 <  1:1867428/100‑2 (MQ=255)

                                            cAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTcc                                                 <  2:1951307/100‑1 (MQ=255)

                                             aaTCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCt                                                <  1:1907040/100‑1 (MQ=255)

                                             aaTCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCt                                                <  1:1641021/100‑1 (MQ=255)

                                             aaTCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCt                                                >  2:3273443/1‑100 (MQ=255)

                                             aaTCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCt                                                >  2:1703167/1‑100 (MQ=255)

                                              aTCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTg                                               >  2:2480713/1‑100 (MQ=255)

                                               tCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGa                                              <  2:341817/100‑1 (MQ=255)

                                                cGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTg                                               <  2:784103/98‑1 (MQ=255)

                                                cGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGaa                                             >  1:3301383/1‑100 (MQ=255)

                                                 gTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGaaa                                            <  2:3497753/100‑1 (MQ=255)

                                                 gTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGaaa                                            >  1:525080/1‑100 (MQ=255)

                                                 gTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGaaa                                            <  1:1527807/100‑1 (MQ=255)

                                                  tAAACATCAGCTTGTAAGCGCTAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGaaaa                                           >  2:2993301/1‑100 (MQ=255)

                                                       aTCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTAtt                                      >  2:2326235/1‑100 (MQ=255)

                                                        tCAGCTTGTAAGCGAGAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGTAGCATTCATATAGGCGTTTCCTGAAAACTAttt                                     <  1:3361473/100‑1 (MQ=255)

                                                          aGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCa                                   <  1:414324/100‑1 (MQ=255)

                                                          aGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCa                                   <  2:823345/100‑1 (MQ=255)

                                                          aGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCa                                   >  2:1976924/1‑100 (MQ=255)

                                                          aGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCa                                   <  1:797641/100‑1 (MQ=255)

                                                          aGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCa                                   <  1:2916911/100‑1 (MQ=255)

                                                           gCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCaa                                  <  1:3406399/100‑1 (MQ=255)

                                                            cTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAg                                 <  2:2124226/100‑1 (MQ=255)

                                                            cTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAg                                 >  2:2168542/1‑100 (MQ=255)

                                                            cTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAg                                 >  2:2741770/1‑100 (MQ=255)

                                                             ttGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGc                                <  2:2630154/100‑1 (MQ=255)

                                                              tGTAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCg                               >  1:3446867/1‑100 (MQ=255)

                                                                tAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGAt                             >  1:20970/1‑100 (MQ=255)

                                                                tAAGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGAt                             <  1:2088404/100‑1 (MQ=255)

                                                                 aaGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATGTAGGCGTTTCCTGAAAACTATTTCAAGCGATa                            >  2:1868752/1‑100 (MQ=255)

                                                                 aaGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATa                            <  2:1273170/100‑1 (MQ=255)

                                                                  aGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAg                           >  2:1515453/1‑100 (MQ=255)

                                                                  aGCGATAGAGTGCTTAAGAGCTTCTACGATAAGCGTGGGCGAGGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAg                           >  1:1125052/1‑100 (MQ=255)

                                                                         gagTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCt                    <  1:1916650/100‑1 (MQ=255)

                                                                         gagTGCTTAAGAGCTTCTACGATAAGCGTGGCCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTACAGc                     >  1:440238/1‑99 (MQ=255)

                                                                          agTGCTCAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTc                   >  2:2257149/1‑100 (MQ=255)

                                                                           gTGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCa                  <  2:1195007/100‑1 (MQ=255)

                                                                            tGCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCAc                 <  2:1753333/100‑1 (MQ=255)

                                                                             gCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACg                >  1:569500/1‑100 (MQ=255)

                                                                             gCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACg                >  2:2171346/1‑100 (MQ=255)

                                                                             gCTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACg                <  1:3364606/100‑1 (MQ=255)

                                                                              cTTAAGATCTTCTACTATAATCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGATAACTATTTCAAGCGATAGTAAAGCTCACgg               <  1:3422397/100‑1 (MQ=255)

                                                                              cTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACgg               <  2:3319447/100‑1 (MQ=255)

                                                                              cTTAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACgg               <  2:3319642/100‑1 (MQ=255)

                                                                               ttAAGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGt              <  1:1471899/100‑1 (MQ=255)

                                                                                 aagcgtttCTACGATCAGAGTAGGCGCTGAATATGTAATCGGTGCATTCATATCGGCGATTCCTGCAAACTATTTCAAGCAATAGTAAAGCt                    >  1:2533231/7‑92 (MQ=255)

                                                                                 aaGAGCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTAc            >  2:3043846/1‑100 (MQ=255)

                                                                                  agagCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACg           >  1:3604527/1‑100 (MQ=255)

                                                                                      cTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACtt       >  1:2608421/1‑100 (MQ=255)

                                                                                        tCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTcg     <  2:515587/100‑1 (MQ=255)

                                                                                         cTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTcgc    >  1:1680794/1‑100 (MQ=255)

                                                                                         cTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTcgc    <  1:640110/100‑1 (MQ=255)

                                                                                          tACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTcgcc   >  2:590938/1‑100 (MQ=255)

                                                                                          tACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGGACGACTTcgc    <  1:2339316/99‑1 (MQ=255)

                                                                                           aCGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTcgccg  >  2:1453278/1‑100 (MQ=255)

TGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCG  >  NC_000913/3566450‑3566640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`