Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,917,345 G→A G763G (GGG→GGA)  barA → hybrid sensory histidine kinase, in two‑component regulatory system with UvrY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,917,3450GA96.6% 292.8 / ‑1.7 89G763G (GGG→GGA) barAhybrid sensory histidine kinase, in two‑component regulatory system with UvrY
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (44/41);  total (47/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTG  >  NC_000913/2917250‑2917441
                                                                                               |                                                                                                
cTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGa                                                                                              >  1:292686/1‑100 (MQ=255)
  cATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGc                                                                                            >  2:756468/1‑100 (MQ=255)
    tCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGGGCAGGCATGAGCGa                                                                                          >  1:1581181/1‑100 (MQ=255)
     ttccgatcTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGAt                                                                                         <  1:2040379/93‑1 (MQ=255)
     ccaccaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGAt                                                                                         <  2:1029622/100‑1 (MQ=255)
      caccaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGAtt                                                                                        <  2:1801820/100‑1 (MQ=255)
       accaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGCAGCTGCTTGGCGCAGGAATGAGCGATTa                                                                                       >  1:1292028/1‑100 (MQ=255)
       accaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTa                                                                                       <  1:382299/100‑1 (MQ=255)
        ccaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAGCGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTa                                                                                       <  2:566468/99‑1 (MQ=255)
        ccaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTAt                                                                                      >  1:963177/1‑100 (MQ=255)
          aaCTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCt                                                                                    >  1:1465735/1‑100 (MQ=255)
          aaCTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCt                                                                                    >  1:651024/1‑100 (MQ=255)
          aaCTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCt                                                                                    >  1:2181339/1‑100 (MQ=255)
           aCTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTg                                                                                   >  2:1469492/1‑100 (MQ=255)
            cTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTgg                                                                                  <  2:426600/100‑1 (MQ=255)
              cccGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGAtagatcggaa                                                                                >  2:2040364/1‑91 (MQ=255)
              cccGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCg                                                                                >  1:782647/1‑100 (MQ=255)
              cccGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCg                                                                                >  1:476230/1‑100 (MQ=255)
              cccGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCg                                                                                >  2:395133/1‑100 (MQ=255)
               ccGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGa                                                                               >  1:1624193/1‑100 (MQ=255)
                 gCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGaaa                                                                             >  1:1074219/1‑100 (MQ=255)
                     cAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGa                                                                         >  2:1181847/1‑100 (MQ=255)
                     cAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGa                                                                         >  1:1573160/1‑100 (MQ=255)
                     cAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGa                                                                         >  2:67239/1‑100 (MQ=255)
                      aGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGAt                                                                        <  2:1437853/100‑1 (MQ=255)
                      aGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGAt                                                                        <  2:2115425/100‑1 (MQ=255)
                      aGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGAt                                                                        >  1:873680/1‑100 (MQ=255)
                      aGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGAt                                                                        >  2:845699/1‑100 (MQ=255)
                        caacaaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTg                                                                      >  1:1071150/1‑100 (MQ=255)
                          acaaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCAGGCGAAACCGATTgaa                                                                    >  2:1322954/1‑100 (MQ=255)
                           caaACGCCGGTTATCGCGGTAACGGCGCATGCAGTGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTgaag                                                                   >  2:1489092/1‑100 (MQ=255)
                           caaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTgaag                                                                   <  1:933083/100‑1 (MQ=255)
                           caaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTgaag                                                                   >  1:315007/1‑100 (MQ=255)
                           caaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTgaag                                                                   <  2:181400/100‑1 (MQ=255)
                             aaCGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTgaagaa                                                                 >  2:746302/1‑100 (MQ=255)
                              aCGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGaagaag                                                                <  1:1887874/100‑1 (MQ=255)
                                gCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAagag                                                              >  1:488986/1‑100 (MQ=255)
                                gCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAagag                                                              <  2:1984625/100‑1 (MQ=255)
                                gCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAagag                                                              >  2:1333998/1‑100 (MQ=255)
                                 ccGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGc                                                             <  1:845706/100‑1 (MQ=255)
                                 ccGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGc                                                             <  1:1010424/100‑1 (MQ=255)
                                  cGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCg                                                            >  2:259676/1‑100 (MQ=255)
                                   ggTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGa                                                           >  2:2127068/1‑100 (MQ=255)
                                    gTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGAt                                                          <  2:1071143/100‑1 (MQ=255)
                                     ttATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGAtt                                                         <  2:1239790/100‑1 (MQ=255)
                                      tATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTg                                                        >  1:663309/1‑100 (MQ=255)
                                      tATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTg                                                        <  2:1243428/100‑1 (MQ=255)
                                         cgcgGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCAt                                                     <  1:1943198/100‑1 (MQ=255)
                                             gTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAAtt                                                 >  2:2109884/1‑100 (MQ=255)
                                              tAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAAttt                                                <  2:2073646/100‑1 (MQ=255)
                                              tAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAAttt                                                >  2:422872/1‑100 (MQ=255)
                                                aCGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgt                                              <  2:476227/100‑1 (MQ=255)
                                                 cGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgtt                                             <  1:1489101/100‑1 (MQ=255)
                                                 cGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgtt                                             <  2:480413/100‑1 (MQ=255)
                                                   gcgcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgt                                           >  2:2214286/1‑100 (MQ=255)
                                                   gcgcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgt                                           <  1:376202/100‑1 (MQ=255)
                                                     gcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgttg                                         <  2:963170/100‑1 (MQ=255)
                                                     gcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgttg                                         <  1:1181854/100‑1 (MQ=255)
                                                      cATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTgc                                        <  1:1611146/100‑1 (MQ=255)
                                                      cATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTgc                                        <  1:949429/100‑1 (MQ=255)
                                                       aTGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTgcg                                       >  1:33569/1‑100 (MQ=255)
                                                       aTGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTgcg                                       >  1:1719489/1‑100 (MQ=255)
                                                       aTGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTgc                                        <  2:1913249/99‑1 (MQ=255)
                                                        tGCAATGGCCGGGAAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTgcgc                                      >  1:1517237/1‑100 (MQ=255)
                                                          cAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTa                                    >  1:614039/1‑100 (MQ=255)
                                                               gCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGc                               >  1:1632720/1‑100 (MQ=255)
                                                                ccGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGcc                              <  2:1759972/100‑1 (MQ=255)
                                                                  gggCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTg                            >  1:1201088/1‑100 (MQ=255)
                                                                   ggCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGCTTGCATAATTTGTTGTTGCGCTACAAGCCTgg                           >  2:225932/1‑100 (MQ=255)
                                                                      aaaaaGAGAAGCTGCTTGGCGCAGGACTGAGAGATTATCTGGCGAAACCGATTGACGACGAGCGATTGCATAATTTGTTGTTGCGCTCCACGCCTGGCAg                        <  2:1581329/100‑1 (MQ=255)
                                                                       aaaaGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGc                       >  2:1801811/1‑100 (MQ=255)
                                                                       aaaaGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGc                       <  2:2181324/100‑1 (MQ=255)
                                                                         aaGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCgg                     <  1:493/100‑1 (MQ=255)
                                                                         aaGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCgg                     >  1:969368/1‑100 (MQ=255)
                                                                            agaAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTAt                  <  1:875503/100‑1 (MQ=255)
                                                                              aaGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTAttt                <  1:1334007/100‑1 (MQ=255)
                                                                              aaGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTAttt                <  1:67241/100‑1 (MQ=255)
                                                                                gctgctTGGCGCAGGAATGAGCGATTATCTGGCGGAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTcc              <  2:292683/100‑1 (MQ=255)
                                                                                gctgctTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTcc              >  2:1696993/1‑100 (MQ=255)
                                                                                gctgctTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTcc              >  2:1697009/1‑100 (MQ=255)
                                                                                caggctTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTcc              <  1:234380/97‑1 (MQ=255)
                                                                                   gctTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCctct           <  2:33569/100‑1 (MQ=255)
                                                                                   gctTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCctct           >  1:1924191/1‑100 (MQ=255)
                                                                                   gctTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCctct           <  1:756475/100‑1 (MQ=255)
                                                                                      tGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTcgc        <  2:1719480/100‑1 (MQ=255)
                                                                                       ggCGCGGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTcgcg       >  1:1871618/1‑100 (MQ=255)
                                                                                       ggCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTcgcg       <  2:1465726/100‑1 (MQ=255)
                                                                                        gcgcAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGcgt      >  2:1957384/1‑100 (MQ=255)
                                                                                            aGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTg  <  2:663304/100‑1 (MQ=255)
                                                                                               |                                                                                                
CTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTG  >  NC_000913/2917250‑2917441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑